Vitro Diabetic(体外糖尿病)研究综述
Vitro Diabetic 体外糖尿病 - Method: We utilize the previously established in vitro diabetic cardiomyopathy model of human induced pluripotent stem cell–derived human cardiomyocytes to perform long non-coding RNA and mRNA expression analysis on glucose (11 mM), endothelin-1 (10 nM) and cortisol (1 µM) stimulated human induced pluripotent stem cell–derived human cardiomyocytes to interrogate diabetic cardiomyopathy associated RNA expression profile. [1] Then, we scrutinized the impact of MSC-CM treatment on fibroblast cellular behavior by culturing human dermal fibroblasts (HDFs) in a high-glucose (HG) medium, as an in vitro diabetic model. [2] In an in vitro diabetic apoptosis cell model, we evaluated the function of long noncoding RNA, insulin growth factor 2 antisense (IGF2‐AS) in high‐glucose‐injured human retinal pigment epithelial cells. [3] Here, we investigated whether ginseng total saponin (GTS) had a protective role in the changes of podocyte CD2AP protein and podocyte apoptosis under in vitro diabetic conditions. [4] The successful in vitro and in vivo delivery of siRNA, as well as targeted gene knockdown potential, was demonstrated by HDAC4 inhibition in vitro diabetic nephropathy (DN) podocyte model as well as in vivo DN C57BL/6 mice model. [5]方法：我们利用先前建立的人诱导多能干细胞衍生的人心肌细胞的体外糖尿病心肌病模型，对葡萄糖（11 mM）、内皮素-1（10 nM）和皮质醇进行长链非编码RNA和mRNA表达分析。 1 µM) 刺激人诱导的多能干细胞衍生的人心肌细胞以询问糖尿病心肌病相关的 RNA 表达谱。 [1] 然后，我们通过在高葡萄糖 (HG) 培养基中培养人真皮成纤维细胞 (HDF) 作为体外糖尿病模型，仔细研究了 MSC-CM 治疗对成纤维细胞行为的影响。 [2] 在体外糖尿病凋亡细胞模型中，我们评估了长链非编码 RNA、胰岛素生长因子 2 反义物 (IGF2-AS) 在高葡萄糖损伤的人视网膜色素上皮细胞中的功能。 [3] 在这里，我们研究了人参总皂苷（GTS）是否在体外糖尿病条件下对足细胞CD2AP蛋白和足细胞凋亡的变化具有保护作用。 [4] 通过体外糖尿病肾病 (DN) 足细胞模型和体内 DN C57BL/6 小鼠模型中的 HDAC4 抑制，证明了 siRNA 的成功体外和体内递送以及靶向基因敲低潜力。 [5]