Spectroscopy Assay(光谱分析)研究综述
Spectroscopy Assay 光谱分析 - OUTCOMES We measured plasma and urine concentrations of nine endogenous secretory solutes using a targeted liquid chromatography mass-spectroscopy assay. [1] Materials and Methods Cur@PLGA-NPs was synthesized using Electrospinning process and characterized by different analysis including Scanning Electron Microscope (SEM), Transmission Electron Microscope (TEM), and Fourier Transform Infrared (FTIR) spectroscopy assays. [2] NIRS-based predictions of concentrations both inside the feed frame and in tablets were compared to ultraviolet (UV) spectroscopy assays of temporally stratified tablet samples. [3] Thus, with single-walled carbon nanotube (SWCNT) treated as model nanomaterials, a combination of in vivo experiments, spectroscopy assay and molecular dynamic modeling was applied to help us unravel some important issues on the binding characterization between SWCNT and the ligand binding domain (LBD) of ER alpha (ERα). [4]结果 我们使用靶向液相色谱质谱测定法测量了九种内源性分泌溶质的血浆和尿液浓度。 [1] 材料和方法 Cur@PLGA-NPs 是使用静电纺丝工艺合成的，并通过不同的分析进行表征，包括扫描电子显微镜 (SEM)、透射电子显微镜 (TEM) 和傅里叶变换红外 (FTIR) 光谱分析。 [2] 将基于 NIRS 的饲料框架内和片剂浓度预测与时间分层片剂样品的紫外 (UV) 光谱分析进行比较。 [3] 因此，将单壁碳纳米管（SWCNT）作为模型纳米材料，结合体内实验、光谱分析和分子动力学建模，帮助我们解开SWCNT与配体结合域之间结合表征的一些重要问题。 LBD) 的 ER α (ERα)。 [4]
Force Spectroscopy Assay
AFM is able to not only visualize the surface topography of the specimens, but also can quantify the mechanical properties of the specimens by force spectroscopy assay. [1] We apply single-molecule force spectroscopy assays to investigate the insertion and folding process of single lactose permease (LacY) precursors assisted by YidC and SecYEG. [2] To expand the single-molecule toolbox, we have developed a surface-free force spectroscopy assay based on a high-speed hydrodynamic trap capable of applying extremely high tensions for long periods of time. [3] Here, we demonstrate a magnetic tweezers force spectroscopy assay that resolves the dynamics of Cpa thioester bond under force. [4]AFM 不仅能够可视化样品的表面形貌，还可以通过力谱分析来量化样品的机械性能。 [1] 我们应用单分子力光谱分析来研究由 YidC 和 SecYEG 辅助的单个乳糖通透酶 (LacY) 前体的插入和折叠过程。 [2] 为了扩展单分子工具箱，我们开发了一种基于能够长时间施加极高张力的高速流体动力学陷阱的无表面力光谱分析。 [3] 在这里，我们展示了一种磁性镊子力谱测定法，可解析 Cpa 硫酯键在力作用下的动力学。 [4]
Mas Spectroscopy Assay 质谱分析
Serum samples were collected weekly for one month after injection and assayed with a liquid chromatography-mass spectroscopy assay. [1] We functionally tested all 64 rare missense mutations in Western Blot and Mass Spectroscopy assays for proatrial natriuretic peptide cleavage. [2] A mass spectroscopy assay to sensitively measure mature frataxin (reduction of which is the root cause of disease) is being developed. [3]在注射后的一个月内每周收集血清样品，并用液相色谱-质谱分析法进行分析。 [1] 我们在蛋白质印迹和质谱分析中对所有 64 种罕见的错义突变进行了功能性测试，用于前心房利钠肽裂解。 [2] 正在开发一种质谱分析法来灵敏地测量成熟的 frataxin（其减少是疾病的根本原因）。 [3]
Fluorescence Spectroscopy Assay
Here, a total of 21 known hGH naturally-occurring missence mutations that have been clinically observed to be involved in IGHD disorder are collected and confirmed by original literature; they effects on the conformation, energetics and dynamics of hGH-hGHbp recognition and interaction are dissected at molecular level by using atomistic dynamics simulations, binding energy calculations and fluorescence spectroscopy assays. [1] Finally, piroxicam was chosen as the repurposing drug to become a new DPP-4 inhibitor and validated experimentally using fluorescence spectroscopy assay. [2]在这里，总共收集了 21 个已知的 hGH 自然发生的错失突变，这些突变已被临床观察到与 IGHD 障碍有关，并得到原始文献的证实；通过使用原子动力学模拟、结合能计算和荧光光谱分析，在分子水平上剖析了它们对 hGH-hGHbp 识别和相互作用的构象、能量学和动力学的影响。 [1] 最后，吡罗昔康被选为再利用药物，成为一种新的 DPP-4 抑制剂，并使用荧光光谱法进行了实验验证。 [2]
Multiple Spectroscopy Assay
This is the first study to combine multiple spectroscopy assays, imaging, and rheology measurements of SA and to demonstrate a resolution on a nanoscale structural toward better resolution of the conformational changes of glycated SA, oligomerization, and protein aggregations under physiological conditions. [1] Furthermore, the DNA-binding behaviours of these complexes were investigated by a multiple spectroscopy assay and viscosity study. [2]这是第一项将 SA 的多种光谱分析、成像和流变测量相结合的研究，并展示了纳米级结构的分辨率，以更好地分辨生理条件下糖化 SA、寡聚化和蛋白质聚集的构象变化。 [1] 此外，通过多重光谱分析和粘度研究研究了这些复合物的 DNA 结合行为。 [2]
Emission Spectroscopy Assay 发射光谱分析
For dilute samples of Fab fragment, a dye fluorescence emission spectroscopy assay was developed to quantitate the number of cocaine (and other high affinity cocaine metabolites) binding sites via the ligand-induced decrease in fluorescence emission of both of these extrinsic dyes. [1] The optical emission spectroscopy assay proved that the LTAP produced by an arc discharge reactor contains reactive oxygen and nitrogen species. [2]对于 Fab 片段的稀释样品，开发了一种染料荧光发射光谱测定法，以通过配体诱导的这两种外在染料的荧光发射减少来量化可卡因（和其他高亲和力可卡因代谢物）结合位点的数量。 [1] 光发射光谱分析证明，由电弧放电反应器产生的 LTAP 含有活性氧和氮物质。 [2]