Shoot Cultures(拍摄文化)研究综述
Shoot Cultures 拍摄文化 - In the present study, the individual and combined effects of a stress tolerance-inducing (salicylic acid) and a stress-inducing elicitor (polyethylene glycol) were evaluated on regeneration efficiency, antioxidants activity and phytochemical profile of in vitro shoot cultures of ajowan. [1] The effectiveness of different elicitation variants in combination with alarmone application was studied in shoot cultures of Polyscias filicifolia. [2] MATERIALS AND METHODS In vitro established shoot cultures were treated with different doses of gamma irradiations (10-100 Gy). [3] Shoot cultures in MS medium without plant growth regulators were maintained under blue (B), red (R), red plus blue (R2B), and white (CW) light spectra (25°C ± 2°C; 50 µmol m -2 s -1 ) for up to 140 days. [4] The aim of this work was to investigate the impact of the addition of copper oxide (CuO), zinc oxide (ZnO) and iron oxide (Fe3O4) NPs (<100 nm) at different concentrations (1, 5 and 10 mg/L) to the culture media on several morphological, physiological and -biochemical parameters of in vitro shoot cultures of Lavandula viridis L’Hér and Thymus lotocephalus G. [5] Identification and quantification of anthocyanins and soluble sugars by the HPLC method in shoot cultures and ex vitro established plantlets were also performed. [6] , polyethylene glycol (PEG), alginate (ALG), chitosan (CHI), salicylic acid (SA), and yeast extract (YE), were used in in vitro shoot cultures of Stevia rebaudiana for 4 wk to decipher their effect on growth, biomass yield, and accumulation of steviol glycosides (SG), especially rebaudiosides. [7] longifolia shoot cultures. [8] Hyperhydricity of shoots initiated in vitro, poor shoot extension, inability of shoot cultures to maintain good growth over an extended time, and unsuccessful ex vitro rooting have limited the development of a commercial scale micropropagation system for hemp (Cannabis sativa). [9] We also found that gene expression levels of miR156, 159 and 1171 was reduced in salicylic acid treated axenic shoot cultures of C. [10] In this context, in vitro shoot cultures were obtained from germinated mature seeds. [11] Given the increasing interest in natural products of the genus Hypericum, knowledge of the spectrum of phenolic compounds in shoot cultures is a prerequisite for future biotechnological applications. [12] This article reports simple, robust, and species-independent methods for establishing in vitro cell suspension and shoot cultures useful for sustainable bioprospecting of Hypericum, a genus containing several medicinal plants commonly used in treating mild and moderate depression. [13] The present study, evaluates ethyl methane sulphonate (EMS) induced variations, in the fatty acids content of the treated shoot cultures against the in vivo/in vitro fatty acids profile of the shoots. [14] Shoot cultures of this plant were established by adding various concentrations of kinetin (Kn) and benzyl adenine (BA) using nodal explants. [15] Callus and shoot cultures derived through leaf and nodal explants respectively were further qualitatively and quantitatively analyzed for their biosynthetic potential. [16] The overall objective of the current study was to investigate the effect of salt stress (100 mm NaCl) on genetic variation in calli and shoot cultures of S. [17] In vitro shoot cultures of common vervain ( Verbena officinalis L. [18] officinale microshoot cultures in vitro. [19] The increased sucrose concentration enhanced accumulation of xanthones in shoot cultures 2–3-fold compared to the control shoots. [20] The effect of addition of Potential Metabolite Stimulants (PMSs) - casein acid hydrolysate, meat peptone, salicylic acid, copper sulphate, and silver nitrate, on the concentrations of these saponins and transcript levels of associated genes encoding important biosynthetic enzymes, was assessed in axenic shoot cultures of C. [21] This research was aimed to accelerate growth of taro shoot cultures by increasing of the vitamin levels added on MS liquid medium using three different ploidy levels of taro. [22] Shoot cultures of Bulgarian accessions of the plant were developed and an experiment for elucidation of the combined effect of vitamins and auxin and cytokinin on their biomass formation, physiological status and secondary metabolite productivity was conducted. [23] malaccensis in vitro shoot cultures and could be used to manipulate the accumulation of different products in culture. [24] The study demonstrated the effects of precursor feeding on the production of glucosinolates (GSLs), flavonoids, polyphenols, saccharides, and photosynthetic pigments in Nasturtium officinale microshoot cultures grown in Plantform bioreactors. [25] Shoot cultures of selected six Musa spp. [26] The present work was aimed at studying the potential of elicitation on the accumulation of phenolic compounds in in vitro shoot cultures of Eryngium alpinum L. [27] The current study involved the induction of agitated micro-shoot cultures with the aim to investigate the growth-promoting as well as phytochemicals enhancement role of yeast extract (YE) and pectin (PE). [28] In this study, in vitro shoot cultures were derived from a single mature embryo (open pollinated) of ‘Selah’ sweet cherry. [29] Shoot cultures of Bacopa were cultivated in two different modified benchtop bioreactors: glass bottle bioreactor and balloon type bubble bioreactor and compared with those grown in traditional Erlenmeyer agitated flask. [30] The extracts from both shoot cultures and the leaves from field-grown plants revealed antioxidant activity and they exhibited moderate antimicrobial activity. [31] This study evaluated the effects of application of ABA (0, 5, 25, 50, and 100 µM) to shoot cultures of lemon balm (Melissa officinalis L. [32] Désirée) shoot cultures and checked for possible connections between the rhythmicity of endogenous phytohormones and phototropic bending capacity of the same cultures. [33] Although it is possible to produce a huge number of plants through tissue culture via embryogenesis or multiple shoot cultures, but their delivery is quite inconvenient. [34] In the present work, we studied responses of in vitro shoot cultures to salt stress (0 (control), 100, 200 and 300 mM NaCl) and salt stress-induced accumulation of 20-hydroxyecdysone (20E). [35] rotundifolia shoot cultures. [36] In the presented research, diverse in vitro cultures of this taxon were developed to obtain the uniform material capable of producing ecdysteroids, including micropropagated plantlets, shoot cultures, liquid agitated whole plant cultures with fast-growing roots, and callus. [37] In this study, the effect of abiotic elicitor (chitosan) at various concentrations on rosmarinic acid (RA) and total phenolic accumulation in shoot cultures of lemon balm was investigated. [38] Therefore, shoot cultures of tested species grown on medium enriched simultaneously with 0. [39] asiatica agitated shoot cultures were established to study the influence of ethephon, methyl jasmonate, L‐phenylalanine (Eth 50 µM, MeJa 50 µM, L‐Phe 2. [40] Here we describe procedures for transient and stable transformation of leaf mesophyll protoplasts obtained from axenic shoot cultures of canola (Brassica napus). [41] The influence of bioreactor type on the accumulation of phenolic acids and flavonoids in microshoot cultures of Schisandra chinensis was proven and optimized. [42] The latter phenomenon was suppressed using the auxin antagonist α-(2-oxo-2-phenylethyl)-1H-indole-3-acetic acid (PEO-IAA), and multiple shoot cultures were established from isolated apical meristems using media containing the novel synthetic cytokinin derivative 6-benzylamino-9-(tetrahydroxypyranyl)purin (BAP9THP). [43] malaccensis shoot cultures were propagated in Murashige and Skoog (MS) semisolid medium and then pre-conditioned in thin layer culture before bioreactor cultivation. [44] A high performance liquid chromatography analysis showed that elicitation with 100 µM concentration of methyl jasmonate enhanced asiaticoside content by 69 fold in callus culture and 39 fold in shoot cultures whereas asiatic acid was enhanced by 1. [45] in vitro shoot cultures were used to study influence of various factors on polyphenol profile using Ultra Performance Liquid Chromatography coupled to high resolution mass spectrometry (UPLC-ESI-qTOF-MS). [46] Compared to the wild grown species, in vitro shoot cultures accumulated double the amount of the main saponin (225. [47] However, the multiple shoot cultures do provide the required cellular complexity to complete the entire TIAs pathway. [48] The aim of our work was to evaluate the effect of selected antibiotics on the growth of potato shoot cultures in the Gene Bank of the Slovak Republic collection and to determine the type and dose that may be used to treat potato cultures endangered by endophytic bacteria. [49] Depending on the hormonal supplementation, the biomass from the shoot cultures accumulated from 11. [50]在本研究中，评估了应激耐受诱导剂（水杨酸）和应激诱导剂（聚乙二醇）对 ajowan 体外芽培养的再生效率、抗氧化活性和植物化学特征的单独和组合影响。 [1] 在 Polyscias filicifolia 的枝条培养中研究了不同诱导变体与警报酮应用相结合的有效性。 [2] 材料和方法 用不同剂量的伽马辐射 (10-100 Gy) 处理体外建立的芽培养物。 [3] 在蓝色 (B)、红色 (R)、红色加蓝色 (R2B) 和白色 (CW) 光谱 (25°C ± 2°C; 50 µmol m -2) 下，在不含植物生长调节剂的 MS 培养基中培养芽s -1 ) 最长 140 天。 [4] 这项工作的目的是研究添加不同浓度（1、5 和 10 mg/L）的氧化铜 (CuO)、氧化锌 (ZnO) 和氧化铁 (Fe3O4) NPs (<100 nm) 的影响关于 Lavandula viridis L'Hér 和 Thymus lotocephalus G 体外芽培养的几个形态学、生理学和生化参数的培养基。 [5] 还通过 HPLC 方法在芽培养和体外建立的小植物中鉴定和定量花青素和可溶性糖。 [6] 、聚乙二醇 (PEG)、海藻酸盐 (ALG)、壳聚糖 (CHI)、水杨酸 (SA) 和酵母提取物 (YE)，用于甜叶菊的体外枝条培养 4 周，以破译它们对生长的影响，生物量产量和甜菊糖苷（SG）的积累，尤其是莱鲍迪苷。 [7] 长叶芽培养。 [8] 体外启动的枝条过度含水、枝条延伸不良、枝条培养物无法在较长时间内保持良好的生长以及体外生根不成功，这些都限制了大麻（大麻）商业规模微繁殖系统的发展。 [9] 我们还发现 miR156、159 和 1171 的基因表达水平在水杨酸处理的 C. [10] 在这种情况下，体外芽培养物是从发芽的成熟种子中获得的。 [11] 鉴于对金丝桃属天然产物的兴趣日益增加，了解枝条培养物中酚类化合物的光谱是未来生物技术应用的先决条件。 [12] 本文报告了建立体外细胞悬浮和芽培养的简单、稳健和不依赖物种的方法，这些方法可用于金丝桃属植物的可持续生物勘探，金丝桃属植物含有几种通常用于治疗轻度和中度抑郁症的药用植物。 [13] 本研究评估了甲基磺酸乙酯 (EMS) 诱导的变化，在处理过的枝条培养物中的脂肪酸含量与枝条的体内/体外脂肪酸谱的对比。 [14] 通过使用节外植体添加不同浓度的激动素 (Kn) 和苄基腺嘌呤 (BA) 来建立这种植物的枝条培养物。 [15] 分别通过叶和节外植体获得的愈伤组织和芽培养物进一步定性和定量分析它们的生物合成潜力。 [16] 本研究的总体目标是研究盐胁迫（100 mm NaCl）对 S. 愈伤组织和芽培养物遗传变异的影响。 [17] 普通马鞭草 (Verbena officinalis L. [18] officinale microshoot 体外培养。 [19] 与对照枝条相比，增加的蔗糖浓度增加了枝条培养物中呫吨酮的积累 2-3 倍。 [20] 在无菌条件下评估了添加潜在代谢物兴奋剂 (PMS) - 酪蛋白酸水解物、肉蛋白胨、水杨酸、硫酸铜和硝酸银对这些皂苷浓度和编码重要生物合成酶的相关基因转录水平的影响C. 拍摄文化 [21] 本研究旨在通过使用三种不同倍性水平的芋头增加 MS 液体培养基中添加的维生素水平来加速芋头培养物的生长。 [22] 开发了保加利亚植物种质的枝条培养物，并进行了一项实验，以阐明维生素、生长素和细胞分裂素对其生物量形成、生理状态和次生代谢产物生产力的综合影响。 [23] malaccensis 体外芽培养，可用于控制培养中不同产物的积累。 [24] 该研究证明了前体喂养对在 Plantform 生物反应器中生长的金莲花微芽培养物中硫代葡萄糖苷 (GSL)、类黄酮、多酚、糖类和光合色素产生的影响。 [25] 选择六种Musa spp的射击文化。 [26] 目前的工作旨在研究激发刺参体外芽培养中酚类化合物积累的潜力。 [27] 目前的研究涉及搅拌微芽培养物的诱导，旨在研究酵母提取物 (YE) 和果胶 (PE) 的生长促进作用和植物化学物质增强作用。 [28] nan [29] nan [30] nan [31] nan [32] nan [33] nan [34] nan [35] nan [36] nan [37] nan [38] nan [39] nan [40] nan [41] nan [42] nan [43] nan [44] nan [45] nan [46] nan [47] nan [48] nan [49] nan [50]
Vitro Shoot Cultures
In the present study, the individual and combined effects of a stress tolerance-inducing (salicylic acid) and a stress-inducing elicitor (polyethylene glycol) were evaluated on regeneration efficiency, antioxidants activity and phytochemical profile of in vitro shoot cultures of ajowan. [1] The aim of this work was to investigate the impact of the addition of copper oxide (CuO), zinc oxide (ZnO) and iron oxide (Fe3O4) NPs (<100 nm) at different concentrations (1, 5 and 10 mg/L) to the culture media on several morphological, physiological and -biochemical parameters of in vitro shoot cultures of Lavandula viridis L’Hér and Thymus lotocephalus G. [2] , polyethylene glycol (PEG), alginate (ALG), chitosan (CHI), salicylic acid (SA), and yeast extract (YE), were used in in vitro shoot cultures of Stevia rebaudiana for 4 wk to decipher their effect on growth, biomass yield, and accumulation of steviol glycosides (SG), especially rebaudiosides. [3] In this context, in vitro shoot cultures were obtained from germinated mature seeds. [4] In vitro shoot cultures of common vervain ( Verbena officinalis L. [5] malaccensis in vitro shoot cultures and could be used to manipulate the accumulation of different products in culture. [6] The present work was aimed at studying the potential of elicitation on the accumulation of phenolic compounds in in vitro shoot cultures of Eryngium alpinum L. [7] In this study, in vitro shoot cultures were derived from a single mature embryo (open pollinated) of ‘Selah’ sweet cherry. [8] In the present work, we studied responses of in vitro shoot cultures to salt stress (0 (control), 100, 200 and 300 mM NaCl) and salt stress-induced accumulation of 20-hydroxyecdysone (20E). [9] in vitro shoot cultures were used to study influence of various factors on polyphenol profile using Ultra Performance Liquid Chromatography coupled to high resolution mass spectrometry (UPLC-ESI-qTOF-MS). [10] Compared to the wild grown species, in vitro shoot cultures accumulated double the amount of the main saponin (225. [11]在本研究中，评估了应激耐受诱导剂（水杨酸）和应激诱导剂（聚乙二醇）对 ajowan 体外芽培养的再生效率、抗氧化活性和植物化学特征的单独和组合影响。 [1] 这项工作的目的是研究添加不同浓度（1、5 和 10 mg/L）的氧化铜 (CuO)、氧化锌 (ZnO) 和氧化铁 (Fe3O4) NPs (<100 nm) 的影响关于 Lavandula viridis L'Hér 和 Thymus lotocephalus G 体外芽培养的几个形态学、生理学和生化参数的培养基。 [2] 、聚乙二醇 (PEG)、海藻酸盐 (ALG)、壳聚糖 (CHI)、水杨酸 (SA) 和酵母提取物 (YE)，用于甜叶菊的体外枝条培养 4 周，以破译它们对生长的影响，生物量产量和甜菊糖苷（SG）的积累，尤其是莱鲍迪苷。 [3] 在这种情况下，体外芽培养物是从发芽的成熟种子中获得的。 [4] 普通马鞭草 (Verbena officinalis L. [5] malaccensis 体外芽培养，可用于控制培养中不同产物的积累。 [6] 目前的工作旨在研究激发刺参体外芽培养中酚类化合物积累的潜力。 [7] nan [8] nan [9] nan [10] nan [11]
Multiple Shoot Cultures
Although it is possible to produce a huge number of plants through tissue culture via embryogenesis or multiple shoot cultures, but their delivery is quite inconvenient. [1] The latter phenomenon was suppressed using the auxin antagonist α-(2-oxo-2-phenylethyl)-1H-indole-3-acetic acid (PEO-IAA), and multiple shoot cultures were established from isolated apical meristems using media containing the novel synthetic cytokinin derivative 6-benzylamino-9-(tetrahydroxypyranyl)purin (BAP9THP). [2] However, the multiple shoot cultures do provide the required cellular complexity to complete the entire TIAs pathway. [3]Axenic Shoot Cultures
We also found that gene expression levels of miR156, 159 and 1171 was reduced in salicylic acid treated axenic shoot cultures of C. [1] The effect of addition of Potential Metabolite Stimulants (PMSs) - casein acid hydrolysate, meat peptone, salicylic acid, copper sulphate, and silver nitrate, on the concentrations of these saponins and transcript levels of associated genes encoding important biosynthetic enzymes, was assessed in axenic shoot cultures of C. [2] Here we describe procedures for transient and stable transformation of leaf mesophyll protoplasts obtained from axenic shoot cultures of canola (Brassica napus). [3]我们还发现 miR156、159 和 1171 的基因表达水平在水杨酸处理的 C. [1] 在无菌条件下评估了添加潜在代谢物兴奋剂 (PMS) - 酪蛋白酸水解物、肉蛋白胨、水杨酸、硫酸铜和硝酸银对这些皂苷浓度和编码重要生物合成酶的相关基因转录水平的影响C. 拍摄文化 [2] nan [3]