Replication Dynamics(复制动态)研究综述
Replication Dynamics 复制动态 - The ability to monitor DNA replication fork directionality at the genome-wide scale is paramount for a greater understanding of how genetic and environmental perturbations can impact replication dynamics in human cells. [1] Although much is known about IAV replication, the regulation of IAV replication dynamics is not completely understood. [2] Whether this wave contributes to replication dynamics remained unknown. [3] Although oncogene activation significantly alters DNA replication dynamics, causing replication stress and genome instability, little is known about DNA polymerase expression and regulation in response to oncogene activation. [4] We can readily integrate the immune response components of our model into other modeling and simulation frameworks of viral infection dynamics that do detailed modeling of other mechanisms like viral internalization and intracellular viral replication dynamics, which are not explicitly represented in the ODE model. [5] Replication dynamics can hence be observed in genome sequence data by analyzing DNA copy number along chromosomes while accounting for other sources of sequence coverage variation. [6] Eight of these DNAm sites occurred in genes linked to HIV-1 replication dynamics including (SEPSECS, cg19113954), (MALT1, cg15968021), (CPT1C, cg14318858), (CRTAM, cg10977115), (B4GALNT4, cg04663285), (IL10, cg16284789), (TFPI2, cg19645693), and (LIFR, cg26437306); with the remaining sites at intergenic regions containing regulatory elements. [7] To characterize viral replication dynamics in mice, 501Y. [8] In this article, we attempted to take an in-depth look at the dynamics of regeneration from the effects of replication inhibition and cell cycle checkpoint overriding causing premature chromosome condensation (PCC) in terms of DNA damage repair and changes in replication dynamics. [9] Homeostasis in adult tissues relies on the replication dynamics of stem cells, their progenitors and the spatial balance between them. [10] Differences in clinical signs, pulmonary infiltrates, and virus replication dynamics were limited between age cohorts. [11] Resistance mutations to monoclonal antibody (mAb) therapy has been reported, but in the non-immunosuppressed population, it is unclear if in vivo emergence of SARS-CoV-2 resistance mutations alters either viral replication dynamics or therapeutic efficacy. [12] We can readily integrate the immune response components of our model into other modeling and simulation frameworks of viral infection dynamics that do detailed modeling of other mechanisms like viral internalization and intracellular viral replication dynamics, which are not explicitly represented in the ODE model. [13] While CV3-13 did not alter the replication dynamics of SARS-CoV-2 in a K18-hACE2 transgenic mouse model, an Fc-enhanced CV3-13 significantly delayed neuroinvasion and death in prophylactic settings. [14] Similarly, the factors that regulate DNA replication dynamics are poorly understood. [15] Using DNA combing and single cell sequencing, we show that replication forks are unstable, perturbing DNA replication dynamics and generating under- and over-replicated regions at the end of S-phase. [16] However, these usually concentrate on positioning individual events, which only gives an overview of the replication dynamics. [17] Inspired by this observation, we aimed to determine whether similarly increased Polδ levels influence replication dynamics in vivo using the fission yeast Schizosaccharomyces pombe as a model system. [18] Background Measuring DNA replication dynamics with high throughput and single-molecule resolution is critical for understanding both the basic biology behind how cells replicate their DNA and how DNA replication can be used as a therapeutic target for diseases like cancer. [19] Here, we use polymerase usage sequencing to visualize in vivo replication dynamics of HR-restarted forks at an S. [20] Here we report unexpected molecular effects of cATRi on replication dynamics. [21] Here, we developed a single-cell-resolution microfluidic mycobacterial culture device that allows time-lapse microscopy-based long-term phenotypic visualization of the live replication dynamics of mycobacteria. [22] Our approach has the potential to elucidate replication dynamics in E. [23] However, information on viral replication dynamics obtained from traditional bulk assays with host cell populations is inherently limited as the data represent averages over a multitude of unsynchronized replication cycles. [24] Using experimental data on the fission yeast genome, model simulations show how different regions respond to re-replication and permit insight into the key mechanisms affecting re-replication dynamics. [25] However, we understand that further studies are needed to investigate the relationship between viroplasm-like structures and ZIKV replication dynamics. [26] However, BTV replication dynamics in KC cells were not suitable for predicting the replication ability of these virus strains in Culicoides midges. [27] Inspired by this observation, we aimed to determine whether similarly increased Polδ levels influence replication dynamics in vivo using the fission yeast Schizosaccharomyces pombe as a model system. [28] While decades of research have elucidated many steps of the alphavirus lifecycle, the earliest replication dynamics have remained unclear. [29] These effects correlate with altered transcription and replication dynamics in ARID1A knockout cells and to reduced TOP2A binding at R-loop sites. [30] Any obstacle that slows down replication forks or perturbs replication dynamics is generally considered to be a form of replication stress, and the past decade has seen numerous advances in our understanding of how cells respond to and resolve such challenges. [31] • The DP can induce variation in DNA replication dynamics compared to PDP. [32] ALI-PTEC and PBEC were differentiated for 3, 4 or 5 weeks, and next infected with SARS-CoV-2 to assess the impact of differentiation status on replication dynamics. [33] Together, these data reveal novel insight into RV-C15 replication dynamics and resulting cytopathic effects in the primary target cells for infection, thereby furthering our understanding of the pathogenesis of RV-C. [34] To examine replication dynamics displayed by viruses faced with this variable landscape, we have developed a method for fitting a stochastic mechanistic model of viral infection to time-lapse imaging data from high-throughput single-cell poliovirus infection experiments. [35] The different replication dynamics of the virus between the two host species reflect their variation in terms of susceptibility to the virus infection, posing a potential threat to the health of the Western honey bee. [36] Here we report unexpected molecular effects of cATRi on replication dynamics. [37] This chapter outlines the use of the DNA fiber spreading technique for the analysis of replication dynamics and replication structures. [38] Yet infectivity studies in New World primates indigenous to Zika-endemic regions including host range, susceptibility, replication dynamics, tissue tropism and virus persistence are currently lacking. [39] A plaque assay performed with infected mosquito homogenates revealed that CHIKV replication dynamics was similar in Aedes sp. [40] To distinguish between three types of Siniperca chuatsi rhabdovirus (SCRV) viral RNA (vRNA, cRNA, and mRNA) and investigate SCRV transcription and replication dynamics in Chinese perch brain CPB cells, a novel, strand-specific, reverse transcriptase quantitative real-time PCR (RT-qPCR) assay was established. [41] We found that increases in the number of mosquito bites delivered resulted in significantly different virus replication dynamics with higher, earlier peak titers. [42] Replication of eukaryotic genomes is highly stochastic, making it difficult to determine the replication dynamics of individual molecules with existing methods. [43] IL-15 inhibition does not alter SIV replication dynamics or CD4+ T cell depletion. [44] Single-cell analysis of the impact of the viral load on replication dynamics demonstrates the utility of this times-tamping method for tracking the infection cycle. [45] In conclusion, co-infection of chicks with AIV and IBV, simultaneously or sequentially, affected the clinical signs, the virus replication dynamics as well as the internal organ integrity. [46] Deep-sequencing revealed that S10 relocation altered chromosomal replication dynamics and genome-wide transcription. [47] Over the past 50 years or so, studies involving DNA autoradiography in bacterial cells have led to sophisticated DNA tract analyses in human cells to characterize replication dynamics at the single-molecule level. [48] This system allows head-to-head comparisons of STV and DIP replication dynamics over extended time periods. [49] Our findings suggest that transcription has influenced evolution of the binding properties of ORC and replication dynamics in eukaryotes. [50]在全基因组范围内监测 DNA 复制叉方向性的能力对于更好地了解遗传和环境扰动如何影响人类细胞中的复制动态至关重要。 [1] 尽管对 IAV 复制了解很多，但对 IAV 复制动态的调节还没有完全了解。 [2] 这一波是否有助于复制动态仍然未知。 [3] 尽管癌基因激活显着改变了 DNA 复制动力学，导致复制压力和基因组不稳定，但对于 DNA 聚合酶的表达和响应癌基因激活的调节知之甚少。 [4] 我们可以很容易地将我们模型的免疫反应组件集成到病毒感染动力学的其他建模和模拟框架中，这些框架对病毒内化和细胞内病毒复制动力学等其他机制进行详细建模，这些机制在 ODE 模型中没有明确表示。 [5] 因此，通过分析染色体上的 DNA 拷贝数，同时考虑其他序列覆盖变化的来源，可以在基因组序列数据中观察复制动态。 [6] 这些 DNAm 位点中有 8 个发生在与 HIV-1 复制动力学相关的基因中，包括 (SEPSECS, cg19113954), (MALT1, cg15968021), (CPT1C, cg14318858), (CRTAM, cg10977115), (B4GALNT4, cg04663285), (IL10, cg16284789) ), (TFPI2, cg19645693), 和 (LIFR, cg26437306);其余位点位于含有调控元件的基因间区域。 [7] 为了表征小鼠中的病毒复制动力学，501Y。 [8] 在本文中，我们试图从 DNA 损伤修复和复制动态变化方面的复制抑制和细胞周期检查点覆盖导致过早染色体凝聚 (PCC) 的影响，深入研究再生动态。 [9] 成体组织的稳态依赖于干细胞、它们的祖细胞的复制动态以及它们之间的空间平衡。 [10] 不同年龄组之间临床体征、肺部浸润和病毒复制动力学的差异有限。 [11] 已经报道了对单克隆抗体 (mAb) 治疗的耐药性突变，但在非免疫抑制人群中，尚不清楚体内出现的 SARS-CoV-2 耐药性突变是否会改变病毒复制动力学或治疗效果。 [12] 我们可以很容易地将我们模型的免疫反应组件集成到病毒感染动力学的其他建模和模拟框架中，这些框架对病毒内化和细胞内病毒复制动力学等其他机制进行详细建模，这些机制在 ODE 模型中没有明确表示。 [13] 虽然 CV3-13 在 K18-hACE2 转基因小鼠模型中没有改变 SARS-CoV-2 的复制动力学，但 Fc 增强的 CV3-13 在预防性设置中显着延迟了神经侵袭和死亡。 [14] 同样，人们对调节 DNA 复制动力学的因素知之甚少。 [15] 使用 DNA 梳理和单细胞测序，我们表明复制叉是不稳定的，会扰乱 DNA 复制动力学，并在 S 期结束时产生复制不足和过度复制的区域。 [16] 然而，这些通常集中于定位单个事件，这仅给出了复制动态的概述。 [17] 受此观察的启发，我们旨在使用裂殖酵母粟酒裂殖酵母作为模型系统来确定类似增加的 Polδ 水平是否会影响体内复制动力学。 [18] 背景 以高通量和单分子分辨率测量 DNA 复制动力学对于了解细胞如何复制其 DNA 背后的基本生物学以及 DNA 复制如何用作癌症等疾病的治疗靶标至关重要。 [19] 在这里，我们使用聚合酶使用测序来可视化 HR 重新启动的叉在 S 处的体内复制动态。 [20] 在这里，我们报告了 cATRi 对复制动力学的意外分子效应。 [21] 在这里，我们开发了一种单细胞分辨率微流体分枝杆菌培养装置，该装置允许基于延时显微镜的长期表型可视化分枝杆菌的活复制动力学。 [22] 我们的方法有可能阐明大肠杆菌中的复制动态。 [23] 然而，从传统的宿主细胞群批量检测中获得的病毒复制动力学信息本质上是有限的，因为数据代表了多个不同步复制周期的平均值。 [24] 使用裂变酵母基因组的实验数据，模型模拟显示了不同区域如何响应再复制并允许深入了解影响再复制动力学的关键机制。 [25] 然而，我们知道需要进一步的研究来研究病毒质样结构与 ZIKV 复制动力学之间的关系。 [26] 然而，KC 细胞中的 BTV 复制动力学不适合预测这些病毒株在库蠓蠓中的复制能力。 [27] 受此观察的启发，我们旨在使用裂殖酵母粟酒裂殖酵母作为模型系统来确定类似增加的 Polδ 水平是否会影响体内复制动力学。 [28] 虽然几十年的研究已经阐明了甲病毒生命周期的许多步骤，但最早的复制动态仍不清楚。 [29] 这些效应与 ARID1A 敲除细胞中转录和复制动力学的改变以及 R 环位点的 TOP2A 结合减少有关。 [30] 任何减慢复制叉或扰乱复制动态的障碍通常被认为是复制压力的一种形式，在过去的十年中，我们对细胞如何应对和解决这些挑战的理解取得了许多进展。 [31] • 与PDP 相比，DP 可以诱导DNA 复制动力学的变化。 [32] ALI-PTEC 和 PBEC 分化 3、4 或 5 周，然后感染 SARS-CoV-2，以评估分化状态对复制动态的影响。 [33] 总之，这些数据揭示了对 RV-C15 复制动力学和在感染的主要靶细胞中产生的细胞病变效应的新见解，从而进一步了解了 RV-C 的发病机制。 [34] 为了检查面对这种多变环境的病毒所显示的复制动态，我们开发了一种方法，用于将病毒感染的随机机制模型拟合到来自高通量单细胞脊髓灰质炎病毒感染实验的延时成像数据。 [35] 两种宿主物种之间病毒的不同复制动态反映了它们在病毒感染易感性方面的差异，对西方蜜蜂的健康构成潜在威胁。 [36] 在这里，我们报告了 cATRi 对复制动力学的意外分子效应。 [37] 本章概述了 DNA 纤维扩散技术在复制动力学和复制结构分析中的应用。 [38] nan [39] nan [40] nan [41] nan [42] nan [43] nan [44] nan [45] nan [46] nan [47] nan [48] nan [49] nan [50]
Dna Replication Dynamics
Although oncogene activation significantly alters DNA replication dynamics, causing replication stress and genome instability, little is known about DNA polymerase expression and regulation in response to oncogene activation. [1] Similarly, the factors that regulate DNA replication dynamics are poorly understood. [2] Using DNA combing and single cell sequencing, we show that replication forks are unstable, perturbing DNA replication dynamics and generating under- and over-replicated regions at the end of S-phase. [3] Background Measuring DNA replication dynamics with high throughput and single-molecule resolution is critical for understanding both the basic biology behind how cells replicate their DNA and how DNA replication can be used as a therapeutic target for diseases like cancer. [4] • The DP can induce variation in DNA replication dynamics compared to PDP. [5] We show that the nanopore digital counting approach could capture the DNA replication dynamics in the LAMP reaction and has the potential to be used in a qualitative test as well as in a quantitative test. [6] The ability to measure DNA replication dynamics at genome scale has proven crucial for understanding the mechanisms and cellular consequences of DNA replication timing. [7] The DNA composition and transcriptional activity of these heterochromatin blocks have been studied, but little is known about their DNA replication dynamics and epigenetic composition. [8]尽管癌基因激活显着改变了 DNA 复制动力学，导致复制压力和基因组不稳定，但对于 DNA 聚合酶的表达和响应癌基因激活的调节知之甚少。 [1] 同样，人们对调节 DNA 复制动力学的因素知之甚少。 [2] 使用 DNA 梳理和单细胞测序，我们表明复制叉是不稳定的，会扰乱 DNA 复制动力学，并在 S 期结束时产生复制不足和过度复制的区域。 [3] 背景 以高通量和单分子分辨率测量 DNA 复制动力学对于了解细胞如何复制其 DNA 背后的基本生物学以及 DNA 复制如何用作癌症等疾病的治疗靶标至关重要。 [4] • 与PDP 相比，DP 可以诱导DNA 复制动力学的变化。 [5] nan [6] nan [7] nan [8]
Viral Replication Dynamics
We can readily integrate the immune response components of our model into other modeling and simulation frameworks of viral infection dynamics that do detailed modeling of other mechanisms like viral internalization and intracellular viral replication dynamics, which are not explicitly represented in the ODE model. [1] To characterize viral replication dynamics in mice, 501Y. [2] Resistance mutations to monoclonal antibody (mAb) therapy has been reported, but in the non-immunosuppressed population, it is unclear if in vivo emergence of SARS-CoV-2 resistance mutations alters either viral replication dynamics or therapeutic efficacy. [3] We can readily integrate the immune response components of our model into other modeling and simulation frameworks of viral infection dynamics that do detailed modeling of other mechanisms like viral internalization and intracellular viral replication dynamics, which are not explicitly represented in the ODE model. [4] However, information on viral replication dynamics obtained from traditional bulk assays with host cell populations is inherently limited as the data represent averages over a multitude of unsynchronized replication cycles. [5] CH505 recapitulates the early viral replication dynamics and immunopathogenesis of HIV-1 infection of humans and thus can serve as a new model for HIV-1 pathogenesis, treatment, and prevention research. [6] Finally, after ART interruption, we demonstrate the rapid and, compared to peripheral LNs, earlier seeding of SIV in spleen and mesenteric LNs, thereby emphasizing the importance of these two anatomical sites for viral replication dynamics. [7]我们可以很容易地将我们模型的免疫反应组件集成到病毒感染动力学的其他建模和模拟框架中，这些框架对病毒内化和细胞内病毒复制动力学等其他机制进行详细建模，这些机制在 ODE 模型中没有明确表示。 [1] 为了表征小鼠中的病毒复制动力学，501Y。 [2] 已经报道了对单克隆抗体 (mAb) 治疗的耐药性突变，但在非免疫抑制人群中，尚不清楚体内出现的 SARS-CoV-2 耐药性突变是否会改变病毒复制动力学或治疗效果。 [3] 我们可以很容易地将我们模型的免疫反应组件集成到病毒感染动力学的其他建模和模拟框架中，这些框架对病毒内化和细胞内病毒复制动力学等其他机制进行详细建模，这些机制在 ODE 模型中没有明确表示。 [4] 然而，从传统的宿主细胞群批量检测中获得的病毒复制动力学信息本质上是有限的，因为数据代表了多个不同步复制周期的平均值。 [5] nan [6] nan [7]
Viru Replication Dynamics
Differences in clinical signs, pulmonary infiltrates, and virus replication dynamics were limited between age cohorts. [1] We found that increases in the number of mosquito bites delivered resulted in significantly different virus replication dynamics with higher, earlier peak titers. [2] In conclusion, co-infection of chicks with AIV and IBV, simultaneously or sequentially, affected the clinical signs, the virus replication dynamics as well as the internal organ integrity. [3]不同年龄组之间临床体征、肺部浸润和病毒复制动力学的差异有限。 [1] nan [2] nan [3]
Influence Replication Dynamics
Inspired by this observation, we aimed to determine whether similarly increased Polδ levels influence replication dynamics in vivo using the fission yeast Schizosaccharomyces pombe as a model system. [1] Inspired by this observation, we aimed to determine whether similarly increased Polδ levels influence replication dynamics in vivo using the fission yeast Schizosaccharomyces pombe as a model system. [2]受此观察的启发，我们旨在使用裂殖酵母粟酒裂殖酵母作为模型系统来确定类似增加的 Polδ 水平是否会影响体内复制动力学。 [1] 受此观察的启发，我们旨在使用裂殖酵母粟酒裂殖酵母作为模型系统来确定类似增加的 Polδ 水平是否会影响体内复制动力学。 [2]