Plane Microscopy(平面显微镜)研究综述
Plane Microscopy 平面显微镜 - We introduce a novel 3D microrheology system that combines for the first time Optical Tweezers with Integrated Multiplane Microscopy (OpTIMuM). [1] We introduce recent major roles of volume holography in different imaging modalities, including large-capacity spatial-spectral multiplane microscopy, digital holographic microscopy, and structured Talbot (or speckle) illumination fluorescence imaging. [2] We introduce single-molecule oblique-plane microscopy (obSTORM), which by directly imaging oblique sections of samples with oblique light-sheet illumination offers a deep and volumetric SMLM platform that is convenient for standard tissue samples and small intact animals. [3]我们介绍了一种新颖的 3D 微流变系统，该系统首次将光学镊子与集成多平面显微镜 (OpTIMuM) 相结合。 [1] 我们介绍了体积全息术在不同成像模式中的最新主要作用，包括大容量空间光谱多平面显微镜、数字全息显微镜和结构化 Talbot（或散斑）照明荧光成像。 [2] 我们介绍了单分子斜面显微镜 (obSTORM)，它通过使用斜光片照明直接对样品的斜切片进行成像，提供了一个深度和体积 SMLM 平台，便于标准组织样本和小型完整动物。 [3]
Oblique Plane Microscopy 斜平面显微镜
We present an open access scanned oblique plane microscopy platform Crossbill. [1] The recent development of oblique plane microscopy (OPM) simplifies LSFM design with only one single objective lens, by using off-axis excitation and remote focusing. [2] Oblique plane microscopy (OPM) enables high speed, volumetric fluorescence imaging through a single-objective geometry. [3] Most reports on oblique plane microscopy (OPM) have a limited microscopic field of view (FOV), typically within 1x1 mm2. [4] We developed an approach to study cell shape in 2D versus 3D by measuring cell shape at different depths in collagen using stage-scanning oblique plane microscopy (ssOPM). [5] Oblique plane microscopy (OPM), a variant of LSM, circumvents this limitation by exciting oblique planes and detecting the image through the same microscope objective lens. [6] Here we present scattering oblique plane microscopy as a means of imaging moving scattering particles within a turbid medium with the goal of eventually imaging and characterizing blood cells in vivo flowing in superficial capillaries. [7]我们提出了一个开放存取的扫描斜平面显微镜平台 Crossbill。 [1] 斜平面显微镜 (OPM) 的最新发展通过使用离轴激发和远程聚焦，简化了 LSFM 设计，只需一个物镜。 [2] 斜平面显微镜 (OPM) 通过单物镜几何结构实现高速体积荧光成像。 [3] 大多数关于斜平面显微镜 (OPM) 的报告都具有有限的显微视场 (FOV)，通常在 1x1 mm2 以内。 [4] 我们开发了一种方法来研究 2D 与 3D 中的细胞形状，方法是使用阶段扫描斜平面显微镜 (ssOPM) 测量胶原蛋白中不同深度的细胞形状。 [5] 斜平面显微镜 (OPM) 是 LSM 的一种变体，它通过激发斜平面并通过相同的显微镜物镜检测图像来规避这一限制。 [6] 在这里，我们提出散射斜平面显微镜作为一种在混浊介质中对移动的散射粒子进行成像的手段，目的是最终成像和表征体内在表面毛细血管中流动的血细胞。 [7]