Fret Sensor(品丝传感器)研究综述
Fret Sensor 品丝传感器 - To better understand how these cells repeatedly transit from a resting to active state without undergoing metabolic stress, we monitored their early metabolic response to neurotransmission using ion-sensitive probes and FRET sensors in vitro and in vivo. [1] These hetero-FRET sensors also enable us to develop new spectroscopic approaches for quantifying the energy transfer efficiency and the donor-acceptor distance as a means of elucidating the underlying mechanisms for environmental sensing. [2] 173, a Zn2+ FRET sensor. [3] Here, we investigated the excited-state dynamics of recently developed hetero-FRET sensors (mCerulean3-linker-mCitrine) in homogeneous and heterogeneous environments using time-resolved fluorescence measurements, which are compatible with fluorescence lifetime imaging microscopy (FLIM). [4] FRET sensors hardly achieve visualization of spatiotemporal dynamics of protein activity in vivo. [5] We systematically assessed Arch(D95N), Archon, QuasAr, and the eFRET sensors MacQ-mCitrine and QuasAr-mOrange, in the nematode Caenorhabditis elegans. [6] FRET sensors specific for energy substrates, such as glucose, lactate and pyruvate have been developed and successfully used in vitro and in vivo. [7] Results from experiments with RhoA- and PKA-FRET sensors targeted to different subcellular compartments indicate that AMPH-elicited PKA activation occurs throughout the cell, whereas G13-mediated RhoA activation is concentrated near the endoplasmic reticulum. [8] We systematically assessed Arch(D95N), Archon, and QuasAr, as well as the eFRET sensors MacQ-mCitrine and QuasAr-mOrange, in C. [9] Here we have characterized cell lactate and pyruvate dynamics using the FRET sensors Laconic and Pyronic. [10]为了更好地了解这些细胞如何在不经历代谢压力的情况下反复从静止状态转变为活动状态，我们在体外和体内使用离子敏感探针和 FRET 传感器监测了它们对神经传递的早期代谢反应。 [1] 这些异质 FRET 传感器还使我们能够开发新的光谱方法来量化能量转移效率和供体-受体距离，作为阐明环境传感潜在机制的一种手段。 [2] 173，一个 Zn2+ FRET 传感器。 [3] 在这里，我们使用与荧光寿命成像显微镜 (FLIM) 兼容的时间分辨荧光测量方法研究了最近开发的异质 FRET 传感器 (mCerulean3-linker-mCitrine) 在均质和异质环境中的激发态动力学。 [4] FRET 传感器很难实现体内蛋白质活性时空动态的可视化。 [5] 我们系统地评估了秀丽隐杆线虫中的 Arch(D95N)、Archon、QuasAr 和 eFRET 传感器 MacQ-mCitrine 和 QuasAr-mOrange。 [6] 专门针对葡萄糖、乳酸和丙酮酸等能量底物的 FRET 传感器已被开发并在体外和体内成功使用。 [7] 针对不同亚细胞区室的 RhoA 和 PKA-FRET 传感器的实验结果表明，AMPH 引发的 PKA 激活发生在整个细胞中，而 G13 介导的 RhoA 激活集中在内质网附近。 [8] 我们系统地评估了 C 中的 Arch(D95N)、Archon 和 QuasAr，以及 eFRET 传感器 MacQ-mCitrine 和 QuasAr-mOrange。 [9] 在这里，我们使用 FRET 传感器 Laconic 和 Pyronic 表征了细胞乳酸和丙酮酸动力学。 [10]
Metabolic Fret Sensor 代谢品传感器
We have developed methods to utilize a toolset of metabolic FRET sensors for assessing lactate, pyruvate and 2-oxoglutarate levels of Drosophila tissues in vivo by imaging techniques. [1] We have developed methods to utilize a toolset of metabolic FRET sensors for assessing lactate, pyruvate and 2-oxoglutarate levels of Drosophila tissues in vivo by imaging techniques. [2]我们开发了利用代谢 FRET 传感器工具集通过成像技术评估体内果蝇组织的乳酸、丙酮酸和 2-酮戊二酸水平的方法。 [1] 我们开发了利用代谢 FRET 传感器工具集通过成像技术评估体内果蝇组织的乳酸、丙酮酸和 2-酮戊二酸水平的方法。 [2]
Encoded Fret Sensor 编码音品传感器
Here we use Drosophila expressing genetically-encoded FRET sensors to provide an ex vivo characterization of the MC-T in motor neurons and glial cells from the ventral nerve cord. [1] Here, we use Drosophila expressing genetically encoded FRET sensors to provide an ex vivo characterization of the transport of monocarboxylates in motor neurons and glial cells from the larval ventral nerve cord. [2]在这里，我们使用果蝇表达基因编码的 FRET 传感器来提供来自腹神经索的运动神经元和神经胶质细胞中 MC-T 的离体表征。 [1] 在这里，我们使用果蝇表达基因编码的 FRET 传感器来提供来自幼虫腹神经索的运动神经元和神经胶质细胞中单羧酸盐转运的离体表征。 [2]