Fret Probe(音品探头)研究综述
Fret Probe 音品探头 - The suitability as FRET probes of two bichromophoric 1-deoxydihydroceramides containing a labelled spisulosine derivative as a sphingoid base and two differently ω-labelled fluorescent palmitic acids has been evaluated. [1] Its superior biological stability is attributed to the large steric hindrance of the compact and rigid frame of the SC-FRET probe, which helps prevent intracellular degradation and provides a powerful tool for biomedical research. [2] The FRET probe was very stable under extracellular conditions and it exclusively underwent lysosomal esterase enzymatic biodegradation at the intracellular compartments to release RB. [3] We propose that the fast phase measured with our FRET probes represents the macroscopic rate constant associated with actin-activated rotation of the lever arm during the power stroke in M2β-S1. [4] Recently, FRET probes for acid sphingomyelinase (ASM) have enabled the observation of enzyme activity in intact cells for the first time. [5] In addition, the NBC structure was used as a fluorescent donor for FRET probes for the first time, which expanded the diversity of donors. [6] From this, we designed and synthesized two glycan-based FRET probes, which we used to discover antibodies with innate glycosidase activity and analyze their enzyme kinetics, including mAb 2H1, the most efficient identified to date. [7] The wavelength-dependent studies suggest that the 425-nm excitation and the 475-nm emission of the donor are best suited for quantitative assessment of the energy transfer efficiency and the donor-acceptor distance of these FRET probes. [8] Two types of FRET probes, the Cy3 probe and Cy5 probe, were synthesized using gold nanoparticles (AuNPs) as the quencher and a double-stranded DNA as the bridge between fluorophores and AuNPs. [9] In this review, we provide a first-hand account of the concept and development of cFRET, starting from its place in the broader context of FRET probes and assemblies. [10] The model was tested on wavelength-dependent time-resolved fluorescence anisotropy of hetero-FRET probes (mCerulean3-linker-mCitrine) with variable linkers in both crowded (Ficoll-70) and viscous (glycerol) solutions at room temperature. [11] Here, we report a modular disulfide-linked TAMRA-BODIPY based FRET probe that can be readily synthesized, modified, and conjugated to a cysteine-containing biomolecule to enable real-time monitoring of disulfide cleavage during receptor-mediated endocytosis in cells. [12] To test this hypothesis, here we dissected SOD1 into a set of peptides end-labeled with FRET probes to model the local behavior of the corresponding sequences in the unfolded ensemble. [13] A semisynthetic fluorescent protein assembly-based FRET probe (sFPAP) was proposed for cell membrane protease function assay. [14] When the FRET probe was used in in situ activated sludge, M. [15] The FRET probe was successfully utilized in monitoring ATP hydrolysis by apyrase in aqueous solution. [16] The GO based FRET probe is composed of GO and a self-assembled complex of tryptase-specific recognition peptide chains labeled with isothiocyanate fluorescein. [17]已经评估了两种双发色 1-脱氧二氢神经酰胺作为鞘氨醇碱基的标记 spisulosine 衍生物和两种不同 ω 标记的荧光棕榈酸的 FRET 探针的适用性。 [1] 其卓越的生物稳定性归因于 SC-FRET 探针紧凑和刚性框架的大空间位阻，有助于防止细胞内降解，并为生物医学研究提供了强大的工具。 [2] FRET 探针在细胞外条件下非常稳定，并且它仅在细胞内隔室进行溶酶体酯酶酶促生物降解以释放 RB。 [3] 我们建议用我们的 FRET 探针测量的快相代表与 M2β-S1 动力冲程期间杠杆臂的肌动蛋白激活旋转相关的宏观速率常数。 [4] 最近，酸性鞘磷脂酶 (ASM) 的 FRET 探针首次能够观察完整细胞中的酶活性。 [5] 此外，NBC结构首次用作FRET探针的荧光供体，扩大了供体的多样性。 [6] 由此，我们设计并合成了两种基于聚糖的 FRET 探针，我们用它们来发现具有先天糖苷酶活性的抗体并分析它们的酶动力学，包括迄今为止鉴定出的最有效的 mAb 2H1。 [7] 波长相关的研究表明，供体的 425 nm 激发和 475 nm 发射最适合定量评估这些 FRET 探针的能量转移效率和供体-受体距离。 [8] 使用金纳米粒子 (AuNP) 作为猝灭剂和双链 DNA 作为荧光团和 AuNP 之间的桥梁，合成了两种类型的 FRET 探针，即 Cy3 探针和 Cy5 探针。 [9] 在这篇综述中，我们提供了关于 cFRET 概念和发展的第一手资料，从它在 FRET 探针和组件的更广泛背景中的位置开始。 [10] 该模型在室温下在拥挤的 (Ficoll-70) 和粘性 (甘油) 溶液中对具有可变接头的异质 FRET 探针 (mCerulean3-linker-mCitrine) 的波长依赖性时间分辨荧光各向异性进行了测试。 [11] 在这里，我们报告了一种基于模块化二硫键连接的 TAMRA-BODIPY 的 FRET 探针，该探针可以很容易地合成、修饰并与含有半胱氨酸的生物分子结合，从而能够在受体介导的细胞内吞过程中实时监测二硫键的切割。 [12] 为了验证这一假设，我们将 SOD1 分解为一组末端标记有 FRET 探针的肽，以模拟展开集合中相应序列的局部行为。 [13] 提出了一种基于半合成荧光蛋白组装的 FRET 探针 (sFPAP) 用于细胞膜蛋白酶功能测定。 [14] 当 FRET 探针用于原位活性污泥时，M. [15] FRET 探针成功地用于监测水溶液中腺苷三磷酸双磷酸酶的 ATP 水解。 [16] 基于 GO 的 FRET 探针由 GO 和用异硫氰酸酯荧光素标记的类胰蛋白酶特异性识别肽链的自组装复合物组成。 [17]