Fluorescent Reagents(荧光试剂)研究综述
Fluorescent Reagents 荧光试剂 - The observed effects are explained by the realization of charge and hydrophobic matching in the interaction of surfactants with the fluorescent reagents. [1] The influence of charge, hydrophobicity, and spatial characteristics on the analytical signal of cationic and anionic surfactants in their association with fluorescent reagents in aqueous solutions is studied. [2] CS uses a proprietary kit containing a ferrofluid-based reagent, that target CD146 to magnetically capture CECs, and the immunofluorescent reagents to stain the CECs, defined as CD146+, CD105-PE+, DAPI+ and CD45-APC-. [3] The introduction of fluorescent reagents for protein labeling (difference in-gel electrophoresis or DIGE) has brought substantial improvement in this field. [4] Ubiquitin-(Ub) and Ub-like protein-based fluorescent reagents are crucial to explore the activity of Deubiquitinase (DUBs) and to screen its specific inhibitors. [5] When used in conjunction with other fluorescent reagents, new labeling technologies, and tagged reporter constructs, these approaches can generate visually appealing and highly informative insights into diverse aspects of vaccinia virus biology. [6]通过在表面活性剂与荧光试剂的相互作用中实现电荷和疏水匹配来解释观察到的效果。 [1] 研究了电荷、疏水性和空间特性对阳离子和阴离子表面活性剂在水溶液中与荧光试剂结合时分析信号的影响。 [2] CS 使用包含基于铁磁流体的试剂的专有试剂盒，该试剂靶向 CD146 以磁性捕获 CEC，以及用于染色 CEC 的免疫荧光试剂，定义为 CD146+、CD105-PE+、DAPI+ 和 CD45-APC-。 [3] 用于蛋白质标记的荧光试剂（差异凝胶电泳或 DIGE）的引入为该领域带来了实质性的改进。 [4] 泛素 (Ub) 和 Ub 样蛋白基荧光试剂对于探索去泛素酶 (DUB) 的活性和筛选其特异性抑制剂至关重要。 [5] 当与其他荧光试剂、新的标记技术和标记的报告结构结合使用时，这些方法可以对牛痘病毒生物学的各个方面产生具有视觉吸引力和信息量丰富的见解。 [6]