Cucurbit Chlorotic(葫芦科植物)研究综述
Cucurbit Chlorotic 葫芦科植物 - Cucurbit chlorotic yellows virus (CCYV) is a cucurbit-infecting crinivirus. [1] An analysis of the sequences revealed the presence of the criniviruses cucurbit chlorotic yellows virus (CCYV), cucurbit yellow stunting disorder virus (CYSDV), and the begomovirus cucurbit leaf crumple virus (CuLCrV). [2] (2018) revealed several virus-like contigs; among them 23 contigs (206 to 741 nt) shared 98 to 100% nt identities to isolates of cucurbit chlorotic yellows virus (CCYV), genus Crinivirus, family Closteroviridae. [3] In order to identify the cause of the symptoms, RT-PCR was performed using cucurbit chlorotic yellows virus (CCYV) and cucurbit yellow stunting disorder virus (CYSDV) specific primers (Wintermantel et al. [4] 9% sequence identity to distinct cucurbit chlorotic yellows virus (CCYV) isolates (JN641883, RNA1, Taiwan; MH819191, RNA2, China) were also identified. [5] 2008), cucurbit chlorotic yellows virus (CCYV) (Hernandez et al. [6] Two viruses, Rehmannia mosaic virus (ReMV) and cucurbit chlorotic yellows virus (CCYV), were frequently appeared in analyzed six symptomatic samples. [7] In addition, the presence of cucurbit chlorotic yellows virus (CCYV; genus: Crinivirus, family: Closteroviridae) in mixed infection was confirmed in 4/20 samples (3 leaves and 1 fruit) by RT-PCR with primers specific to the CCYV coat protein (CP), heat shock protein 70 homolog (HSP70h) and RNA dependent RNA polymerase (RdRp) designed based on the available CCYV sequences (Sup Table. [8] In 2004, an emergent crinivirus (Cucurbit chlorotic yellows virus, CCYV), inducing symptoms similar to those caused by CYSDV, was described infecting cucurbits in Japan (3). [9] Coinfection of melon by Cucurbit chlorotic yellows virus (CCYV) was recognized in Imperial Valley and neighboring production areas of California and Arizona in 2018, but its importance remains largely unknown. [10] In California, the whitefly-transmitted yellowing viruses, cucurbit yellow stunting disorder virus (CYSDV) and cucurbit chlorotic yellows virus (CCYV), both genus Crinivirus, fam. [11] These foliar symptoms were similar to those caused by the whitefly-transmitted yellowing viruses, cucurbit chlorotic yellows virus (CCYV) and cucurbit yellow stunting disorder virus (CYSDV) (both genus Crinivirus, Closteroviridae). [12] We identified infection by cucurbit chlorotic yellows virus (CCYV) using primers targeting a 953 nt segment of CCYV RNA1 encoding the RNA dependent RNA polymerase gene (RdRp) (CCYV-RDRP-1515F-5'CTCCGAGTAGATCATCCCAAATC3' and CCYV-RDRP-1515R-5'TCACCAGAAACTCCACAATCTC 3') along with other whitefly-transmitted viruses previously reported in Georgia. [13] Cucurbit chlorotic yellows virus (CCYV) has caused serious damage to melon crops in many countries in recent years. [14] BackgroundCucurbit chlorotic yellows virus (CCYV) is a bipartite cucurbit-infecting crinivirus within the family Closteroviridae. [15] Cucurbit yellow stunting disorder virus (CYSDV), Cucurbit chlorotic yellows virus (CCYV) and Beet pseudo-yellows virus (BPYV) are whitefly-transmitted criniviruses that cause foliar interveinal yellowing symptoms and result in high economic losses for cucurbit production. [16] Here, we identified p22 as a silencing suppressor of cucurbit chlorotic yellows crinivirus and showed that p22 interacts with CsSKP1LB1, a Cucumis sativus ortholog of S-phase kinase-associated protein 1 (SKP1). [17] Cucurbit chlorotic yellows virus (CCYV) (genus Crinivirus) is an emergent RNA plant virus and is transmitted specifically by biotypes B and Q of tobacco whitefly, Bemisia tabaci (Gennadius), in a semipersistent manner. [18] Importantly, regardless of temporal and spatial effects, the crinivirus Cucurbit chlorotic yellows virus, which was new to Israeli landscape, was detected in all the tested watermelon plants. [19] In this study, we investigated the RNA silencing suppression activity of several proteins encoded by the RNA1 (RdRp, p22) and RNA2 (CP, CPm and p26) of cucurbit chlorotic yellows virus (CCYV) using co-agroinfiltration assays on Nicotiana benthamiana plants. [20]葫芦科褪绿黄化病毒（CCYV）是一种感染葫芦科的crinivirus。 [1] 对序列的分析显示存在葫芦病毒葫芦褪绿黄化病毒 (CCYV)、葫芦黄色发育迟缓病病毒 (CYSDV) 和贝戈莫病毒葫芦叶皱缩病毒 (CuLCrV)。 [2] (2018) 揭示了几种类似病毒的重叠群；其中 23 个 contigs（206 到 741 nt）与葫芦科黄化病毒（CCYV）、Crinivirus 属、Closteroviridae 家族的分离株具有 98% 到 100% 的 nt 同一性。 [3] 为了确定症状的原因，使用葫芦黄萎黄病毒 (CCYV) 和葫芦黄发育迟缓病病毒 (CYSDV) 特异性引物进行 RT-PCR (Wintermantel et al. [4] nan [5] 2008), 葫芦褪绿黄化病毒 (CCYV) (Hernandez et al. [6] 两种病毒，地黄花叶病毒 (ReMV) 和葫芦褪绿黄化病毒 (CCYV)，经常出现在分析的六个有症状样本中。 [7] 此外，通过使用 CCYV 外壳蛋白特异性引物的 RT-PCR，在 4/20 个样品（3 个叶子和 1 个果实）中证实了混合感染中葫芦科褪绿黄化病毒（CCYV；属：Crinivirus，科：Closteroviridae）的存在(CP)、热休克蛋白 70 同源物 (HSP70h) 和 RNA 依赖性 RNA 聚合酶 (RdRp) 基于可用的 CCYV 序列设计 (Sup Table. [8] 2004 年，一种新出现的 crinivirus（Cucurbit chlorotic yellows virus，CCYV）在日本被描述为感染葫芦科植物（3），该病毒引起的症状与 CYSDV 引起的症状相似。 [9] 2018 年，在帝王谷以及加利福尼亚和亚利桑那州的邻近产区发现了葫芦科褪绿黄病毒 (CCYV) 对甜瓜的共同感染，但其重要性在很大程度上仍然未知。 [10] nan [11] nan [12] 我们使用针对编码 RNA 依赖性 RNA 聚合酶基因 (RdRp) (CCYV-RDRP-1515F-5'CTCCGAGTAGATCATCCCAAATC3' 和 CCYV-RDRP-1515R-5 'TCACCAGAAACTCCACAATCTC 3') 以及之前在乔治亚州报道的其他粉虱传播的病毒。 [13] 近年来，葫芦科黄化病毒（CCYV）对许多国家的甜瓜作物造成了严重破坏。 [14] 背景葫芦褪绿黄化病毒 (CCYV) 是梭状病毒科中的一种二分体感染葫芦科的crinivirus。 [15] 葫芦黄发育迟缓病病毒（CYSDV）、葫芦褪绿黄病毒（CCYV）和甜菜假黄病毒（BPYV）是粉虱传播的克氏病毒，可引起叶脉间黄化症状，给葫芦生产造成高经济损失。 [16] 在这里，我们将 p22 鉴定为葫芦褪绿黄色crinivirus 的沉默抑制因子，并表明 p22 与 CsSKP1LB1 相互作用，CsSKP1LB1 是 S 期激酶相关蛋白 1 (SKP1) 的黄瓜直系同源物。 [17] 葫芦褪绿黄化病毒 (CCYV) (Crinivirus 属) 是一种新兴的 RNA 植物病毒，通过烟草粉虱 Bemisia tabaci (Gennadius) 的生物型 B 和 Q 以半持续性方式特异性传播。 [18] 重要的是，无论时间和空间影响如何，在所有测试的西瓜植物中都检测到了以色列景观中新出现的 crinivirus 葫芦科黄化病毒。 [19] 在这项研究中，我们在本氏烟草植物上使用农用浸润测定法研究了由葫芦科褪绿黄化病毒 (CCYV) 的 RNA1 (RdRp、p22) 和 RNA2 (CP、CPm 和 p26) 编码的几种蛋白质的 RNA 沉默抑制活性。 [20]
cucurbit yellow stunting 葫芦黄色发育迟缓
An analysis of the sequences revealed the presence of the criniviruses cucurbit chlorotic yellows virus (CCYV), cucurbit yellow stunting disorder virus (CYSDV), and the begomovirus cucurbit leaf crumple virus (CuLCrV). [1] In order to identify the cause of the symptoms, RT-PCR was performed using cucurbit chlorotic yellows virus (CCYV) and cucurbit yellow stunting disorder virus (CYSDV) specific primers (Wintermantel et al. [2] In California, the whitefly-transmitted yellowing viruses, cucurbit yellow stunting disorder virus (CYSDV) and cucurbit chlorotic yellows virus (CCYV), both genus Crinivirus, fam. [3] These foliar symptoms were similar to those caused by the whitefly-transmitted yellowing viruses, cucurbit chlorotic yellows virus (CCYV) and cucurbit yellow stunting disorder virus (CYSDV) (both genus Crinivirus, Closteroviridae). [4]对序列的分析显示存在葫芦病毒葫芦褪绿黄化病毒 (CCYV)、葫芦黄色发育迟缓病病毒 (CYSDV) 和贝戈莫病毒葫芦叶皱缩病毒 (CuLCrV)。 [1] 为了确定症状的原因，使用葫芦黄萎黄病毒 (CCYV) 和葫芦黄发育迟缓病病毒 (CYSDV) 特异性引物进行 RT-PCR (Wintermantel et al. [2] nan [3] nan [4]
cucurbit chlorotic yellow 葫芦科褪绿黄
Cucurbit chlorotic yellows virus (CCYV) is a cucurbit-infecting crinivirus. [1] An analysis of the sequences revealed the presence of the criniviruses cucurbit chlorotic yellows virus (CCYV), cucurbit yellow stunting disorder virus (CYSDV), and the begomovirus cucurbit leaf crumple virus (CuLCrV). [2] (2018) revealed several virus-like contigs; among them 23 contigs (206 to 741 nt) shared 98 to 100% nt identities to isolates of cucurbit chlorotic yellows virus (CCYV), genus Crinivirus, family Closteroviridae. [3] In order to identify the cause of the symptoms, RT-PCR was performed using cucurbit chlorotic yellows virus (CCYV) and cucurbit yellow stunting disorder virus (CYSDV) specific primers (Wintermantel et al. [4] 9% sequence identity to distinct cucurbit chlorotic yellows virus (CCYV) isolates (JN641883, RNA1, Taiwan; MH819191, RNA2, China) were also identified. [5] 2008), cucurbit chlorotic yellows virus (CCYV) (Hernandez et al. [6] Two viruses, Rehmannia mosaic virus (ReMV) and cucurbit chlorotic yellows virus (CCYV), were frequently appeared in analyzed six symptomatic samples. [7] In addition, the presence of cucurbit chlorotic yellows virus (CCYV; genus: Crinivirus, family: Closteroviridae) in mixed infection was confirmed in 4/20 samples (3 leaves and 1 fruit) by RT-PCR with primers specific to the CCYV coat protein (CP), heat shock protein 70 homolog (HSP70h) and RNA dependent RNA polymerase (RdRp) designed based on the available CCYV sequences (Sup Table. [8] In 2004, an emergent crinivirus (Cucurbit chlorotic yellows virus, CCYV), inducing symptoms similar to those caused by CYSDV, was described infecting cucurbits in Japan (3). [9] Coinfection of melon by Cucurbit chlorotic yellows virus (CCYV) was recognized in Imperial Valley and neighboring production areas of California and Arizona in 2018, but its importance remains largely unknown. [10] In California, the whitefly-transmitted yellowing viruses, cucurbit yellow stunting disorder virus (CYSDV) and cucurbit chlorotic yellows virus (CCYV), both genus Crinivirus, fam. [11] These foliar symptoms were similar to those caused by the whitefly-transmitted yellowing viruses, cucurbit chlorotic yellows virus (CCYV) and cucurbit yellow stunting disorder virus (CYSDV) (both genus Crinivirus, Closteroviridae). [12] We identified infection by cucurbit chlorotic yellows virus (CCYV) using primers targeting a 953 nt segment of CCYV RNA1 encoding the RNA dependent RNA polymerase gene (RdRp) (CCYV-RDRP-1515F-5'CTCCGAGTAGATCATCCCAAATC3' and CCYV-RDRP-1515R-5'TCACCAGAAACTCCACAATCTC 3') along with other whitefly-transmitted viruses previously reported in Georgia. [13] Cucurbit chlorotic yellows virus (CCYV) has caused serious damage to melon crops in many countries in recent years. [14] BackgroundCucurbit chlorotic yellows virus (CCYV) is a bipartite cucurbit-infecting crinivirus within the family Closteroviridae. [15] Cucurbit yellow stunting disorder virus (CYSDV), Cucurbit chlorotic yellows virus (CCYV) and Beet pseudo-yellows virus (BPYV) are whitefly-transmitted criniviruses that cause foliar interveinal yellowing symptoms and result in high economic losses for cucurbit production. [16] Here, we identified p22 as a silencing suppressor of cucurbit chlorotic yellows crinivirus and showed that p22 interacts with CsSKP1LB1, a Cucumis sativus ortholog of S-phase kinase-associated protein 1 (SKP1). [17] Cucurbit chlorotic yellows virus (CCYV) (genus Crinivirus) is an emergent RNA plant virus and is transmitted specifically by biotypes B and Q of tobacco whitefly, Bemisia tabaci (Gennadius), in a semipersistent manner. [18] Importantly, regardless of temporal and spatial effects, the crinivirus Cucurbit chlorotic yellows virus, which was new to Israeli landscape, was detected in all the tested watermelon plants. [19] In this study, we investigated the RNA silencing suppression activity of several proteins encoded by the RNA1 (RdRp, p22) and RNA2 (CP, CPm and p26) of cucurbit chlorotic yellows virus (CCYV) using co-agroinfiltration assays on Nicotiana benthamiana plants. [20]葫芦科褪绿黄化病毒（CCYV）是一种感染葫芦科的crinivirus。 [1] 对序列的分析显示存在葫芦病毒葫芦褪绿黄化病毒 (CCYV)、葫芦黄色发育迟缓病病毒 (CYSDV) 和贝戈莫病毒葫芦叶皱缩病毒 (CuLCrV)。 [2] (2018) 揭示了几种类似病毒的重叠群；其中 23 个 contigs（206 到 741 nt）与葫芦科黄化病毒（CCYV）、Crinivirus 属、Closteroviridae 家族的分离株具有 98% 到 100% 的 nt 同一性。 [3] 为了确定症状的原因，使用葫芦黄萎黄病毒 (CCYV) 和葫芦黄发育迟缓病病毒 (CYSDV) 特异性引物进行 RT-PCR (Wintermantel et al. [4] nan [5] 2008), 葫芦褪绿黄化病毒 (CCYV) (Hernandez et al. [6] 两种病毒，地黄花叶病毒 (ReMV) 和葫芦褪绿黄化病毒 (CCYV)，经常出现在分析的六个有症状样本中。 [7] 此外，通过使用 CCYV 外壳蛋白特异性引物的 RT-PCR，在 4/20 个样品（3 个叶子和 1 个果实）中证实了混合感染中葫芦科褪绿黄化病毒（CCYV；属：Crinivirus，科：Closteroviridae）的存在(CP)、热休克蛋白 70 同源物 (HSP70h) 和 RNA 依赖性 RNA 聚合酶 (RdRp) 基于可用的 CCYV 序列设计 (Sup Table. [8] 2004 年，一种新出现的 crinivirus（Cucurbit chlorotic yellows virus，CCYV）在日本被描述为感染葫芦科植物（3），该病毒引起的症状与 CYSDV 引起的症状相似。 [9] 2018 年，在帝王谷以及加利福尼亚和亚利桑那州的邻近产区发现了葫芦科褪绿黄病毒 (CCYV) 对甜瓜的共同感染，但其重要性在很大程度上仍然未知。 [10] nan [11] nan [12] 我们使用针对编码 RNA 依赖性 RNA 聚合酶基因 (RdRp) (CCYV-RDRP-1515F-5'CTCCGAGTAGATCATCCCAAATC3' 和 CCYV-RDRP-1515R-5 'TCACCAGAAACTCCACAATCTC 3') 以及之前在乔治亚州报道的其他粉虱传播的病毒。 [13] 近年来，葫芦科黄化病毒（CCYV）对许多国家的甜瓜作物造成了严重破坏。 [14] 背景葫芦褪绿黄化病毒 (CCYV) 是梭状病毒科中的一种二分体感染葫芦科的crinivirus。 [15] 葫芦黄发育迟缓病病毒（CYSDV）、葫芦褪绿黄病毒（CCYV）和甜菜假黄病毒（BPYV）是粉虱传播的克氏病毒，可引起叶脉间黄化症状，给葫芦生产造成高经济损失。 [16] 在这里，我们将 p22 鉴定为葫芦褪绿黄色crinivirus 的沉默抑制因子，并表明 p22 与 CsSKP1LB1 相互作用，CsSKP1LB1 是 S 期激酶相关蛋白 1 (SKP1) 的黄瓜直系同源物。 [17] 葫芦褪绿黄化病毒 (CCYV) (Crinivirus 属) 是一种新兴的 RNA 植物病毒，通过烟草粉虱 Bemisia tabaci (Gennadius) 的生物型 B 和 Q 以半持续性方式特异性传播。 [18] 重要的是，无论时间和空间影响如何，在所有测试的西瓜植物中都检测到了以色列景观中新出现的 crinivirus 葫芦科黄化病毒。 [19] 在这项研究中，我们在本氏烟草植物上使用农用浸润测定法研究了由葫芦科褪绿黄化病毒 (CCYV) 的 RNA1 (RdRp、p22) 和 RNA2 (CP、CPm 和 p26) 编码的几种蛋白质的 RNA 沉默抑制活性。 [20]