Crispr Technologies(Crispr 技术)研究综述
Crispr Technologies Crispr 技术 - It shows promise for functional genomics and the application of CRISPR technologies for the precise improvement of commercial varieties. [1] Timely advancement of the application of CRISPR technologies in plant engineering is crucial for our ability to feed and sustain the growing human population under a changing global climate. [2] Unbiased gene perturbations through RNAi and CRISPR technologies are invaluable tools to establish genotype-to-phenotype relationships at the genome scale. [3] The ever-expanding set of CRISPR technologies and their programmable RNA-guided nucleases exhibit remarkable flexibility in DNA targeting. [4] TALEN have since been largely replaced by the CRISPR technologies which are somewhat easier to build, much easier to multiplex, and have spawned multiple derived techniques. [5] Base editing using CRISPR technologies is an invaluable tool for crop breeding. [6] The aim of this review is to inform future directions in the application of CRISPR technologies in toxicological studies. [7] CRISPR technologies can be harnessed to create rapid, low-cost diagnostic systems, as well as to identify drug-resistance genes. [8] To this aim, we combined optogenetics and CRISPR technologies to activate or knock-down RNA of target genes, at single-cell resolution and in programmable spatial patterns. [9] Despite tremendous pressure on the technology transfer teams, several startups and spin-out companies are already using CRISPR technologies for commercial applications. [10] The Cas9 nuclease from Streptococcus pyogenes (SpCas9) is the most popular enzyme for CRISPR technologies. [11] CRISPR technologies have become standard laboratory tools for genetic manipulations across all kingdoms of life. [12] Our experiments demonstrate that Cpf1 can be efficiently used in Aspergilli for gene editing thereby expanding the range of genomic DNA sequences that can be targeted by CRISPR technologies. [13] CRISPR technologies are nowadays widely used for targeted knockout of numerous protein-coding genes and for the study of various processes and metabolic pathways in human cells. [14] Recent advances in CRISPR technologies have provided new programmable platforms for modifying cell function and behavior. [15] In this review we give an overview of recent advances in identifying novel therapeutic vulnerabilities of AML using CRISPR-Cas9 and discuss possible future applications of CRISPR technologies in this field. [16] Recent developments in CRISPR technologies have opened new possibilities for improving genome editing tools dedicated to the Clostridium genus. [17] Objective: Gather relevant findings from studies that have used CRISPR technologies in zebrafish to explore basic neuronal function and model human diseases. [18] CRISPR-Cas systems naturally rely on CRISPR arrays to achieve immunity against multiple foreign invaders, where these arrays are also being utilized for multiplexed targeting as part of CRISPR technologies. [19] Here we introduce the principles and recent advances of these CRISPR technologies, their delivery vectors and review their applications in stem cell engineering and regenerative medicine. [20] These proteins block different steps of CRISPR-based immunity and, as some inhibit Cas nucleases, can offer tight control over CRISPR technologies. [21]它显示了功能基因组学和 CRISPR 技术在商业品种精确改良方面的应用前景。 [1] 及时推进 CRISPR 技术在植物工程中的应用对于我们在不断变化的全球气候下养活和维持不断增长的人口的能力至关重要。 [2] 通过 RNAi 和 CRISPR 技术进行的无偏基因扰动是在基因组规模上建立基因型与表型关系的宝贵工具。 [3] 不断扩展的 CRISPR 技术及其可编程 RNA 引导的核酸酶在 DNA 靶向方面表现出非凡的灵活性。 [4] 此后，TALEN 已在很大程度上被 CRISPR 技术所取代，这些技术在某种程度上更容易构建，更容易复用，并产生了多种衍生技术。 [5] 使用 CRISPR 技术进行碱基编辑是作物育种的宝贵工具。 [6] 本综述的目的是为 CRISPR 技术在毒理学研究中应用的未来方向提供信息。 [7] CRISPR技术可用于创建快速、低成本的诊断系统，以及识别耐药基因。 [8] 为此，我们将光遗传学和 CRISPR 技术相结合，以单细胞分辨率和可编程空间模式激活或敲低靶基因的 RNA。 [9] 尽管技术转让团队面临巨大压力，但一些初创公司和衍生公司已经将 CRISPR 技术用于商业应用。 [10] 化脓性链球菌 (SpCas9) 中的 Cas9 核酸酶是 CRISPR 技术中最流行的酶。 [11] CRISPR 技术已成为所有生命王国中基因操作的标准实验室工具。 [12] 我们的实验表明，Cpf1 可以有效地用于曲霉进行基因编辑，从而扩大了 CRISPR 技术可以靶向的基因组 DNA 序列的范围。 [13] 如今，CRISPR 技术广泛用于靶向敲除众多蛋白质编码基因以及研究人体细胞中的各种过程和代谢途径。 [14] CRISPR 技术的最新进展为修改细胞功能和行为提供了新的可编程平台。 [15] 在这篇综述中，我们概述了使用 CRISPR-Cas9 识别 AML 新治疗漏洞的最新进展，并讨论了 CRISPR 技术在该领域未来可能的应用。 [16] CRISPR 技术的最新发展为改进梭状芽胞杆菌属的基因组编辑工具开辟了新的可能性。 [17] 目的：从在斑马鱼中使用 CRISPR 技术探索基本神经元功能和模拟人类疾病的研究中收集相关结果。 [18] CRISPR-Cas 系统自然依赖 CRISPR 阵列来实现对多种外来入侵者的免疫，这些阵列也被用于作为 CRISPR 技术的一部分的多重靶向。 [19] 在这里，我们介绍了这些 CRISPR 技术的原理和最新进展、它们的传递载体，并回顾了它们在干细胞工程和再生医学中的应用。 [20] 这些蛋白质阻断基于 CRISPR 的免疫的不同步骤，并且由于一些抑制 Cas 核酸酶，可以对 CRISPR 技术进行严格控制。 [21]
Emerging Crispr Technologies
Future perspectives are opened by emerging CRISPR technologies that could enable genome engineering at larger scale. [1] Emerging CRISPR technologies are enabling powerful new approaches to control mammalian cell functions, yet the lack of spatially-defined, noninvasive modalities to direct their function limit their potential as biological tools and pose a major challenge for clinical translation. [2]新兴的 CRISPR 技术打开了未来的前景，这些技术可以实现更大规模的基因组工程。 [1] 新兴的 CRISPR 技术使控制哺乳动物细胞功能的强大新方法成为可能，但缺乏空间定义的非侵入性方式来指导其功能限制了它们作为生物学工具的潜力，并对临床转化构成重大挑战。 [2]