Cowpea Chlorotic(豇豆褪绿)研究综述
Cowpea Chlorotic 豇豆褪绿 - Among nanocarriers that can be used to achieve nucleic acid delivery, virus-like particles (VLPs) obtained from the Cowpea chlorotic mottle virus (CCMV) are an appealing platform, because of their loading capacity, ease of manufacture and amenability for functionalization. [1] In this study, in order to solve the problem of low expression and low assembly efficiency of VLPs, the conditions for the assembly and purification of eight representative VLPs (hepatitis B virus core antigen protein particles, Qbeta phage, MS2 phage, P22 phage, cowpea chlorotic mottle virus, tobacco Mosaic virus, ferritin and encapsulin) expressed in Escherichia coli were optimized. [2] PVENVs have been developed using several plant viruses, such as cowpea chlorotic mottle virus, potato virus X, cowpea mosaic virus, tobacco mosaic virus, and Brome mosaic virus; these plant viruses are widely used to engineer nanomaterials conjugated with a wide range of biocompatible molecules. [3] For this purpose, we investigated the functionalization capacity of the capsid of cowpea chlorotic mottle virus (CCMV), modified at the interior with a stabilizing elastin-like polypeptide (ELP) tag, by employing a combination of protein engineering and bio-orthogonal chemistry. [4] Here we study how the number of labels per nanoparticle influences the total brightness of fluorescently labelled cowpea chlorotic mottle virus (CCMV). [5] We have previously investigated the charge on the unenveloped plant virus Cowpea Chlorotic Mottle Virus (CCMV) by measurements of its electrophoretic mobility. [6] Of all viruses detected, only two viruses, cucumber mosaic virus and a novel bromovirus related to cowpea chlorotic mottle virus and brome mosaic virus, were mechanically transmitted from wild plants to common bean plants. [7] A model virus, cowpea chlorotic mottle virus, was used and spiked to porcine semen samples. [8] Herein, highly stable NDs encapsulated with Cowpea chlorotic mottle virus capsid proteins (ND-CP) are prepared. [9] To overcome the lack of proper internal and external positive controls and the instability of the detection RNA, we developed virus-like particles (VLPs) using bacteriophage Qβ and plant virus cowpea chlorotic mottle virus (CCMV) for the encapsidation of target RNA, namely a so-called SARS-CoV-2 LAMP detection module (SLDM). [10] A heparin-specific binding peptide was conjugated to a cowpea chlorotic mottle virus (CCMV) capsid protein, which was subsequently allowed to encapsulate heparin and form capsid-like protein cages. [11] VLPs derived from the plant Bromovirus genus, specifically cowpea chlorotic mottle virus (CCMV), are T = 3 icosahedral particles. [12] In particular, VLPs derived from a virus that infects plants, such as cowpea chlorotic mottle virus (CCMV), in which the capsid protein (CP) encapsidates the dsRNA of 563 bp, are shown to silence the WSSV glycoprotein VP28 (dsRNAvp28). [13] Therefore, we studied the assembly of CPs of the cowpea chlorotic mottle virus (CCMV) templated by polyanionic species (cargo), that is, single-stranded DNA (ssDNA), and polystyrene sulfonate (PSS) using isothermal titration calorimetry. [14] We developed a plant viral siRNA delivery platform making use of self-assembling cowpea chlorotic mottle virus (CCMV). [15] Other emerging plant virus-deduced systems include the usually isometric cowpea chlorotic mottle virus (CCMV) with further strikingly altered structures up to "cherrybombs" with protruding nucleic acids. [16] These nanoreactors are made by encapsulating various different enzymes in the protein capsid of the cowpea chlorotic mottle virus (CCMV). [17] Here we report the assembly of capsid proteins of the cowpea chlorotic mottle virus (CCMV) around DNA into defined structures at neutral pH. [18] Purified capsid protein from the plant virus Cowpea Chlorotic Mottle Virus (CCMV) is used to in vitro assemble monodisperse virus-like particles (VLPs) containing reporter proteins (e. [19] Here, we investigate the effect of point-mutations on previously predicted hot-spots of the icosahedral Cowpea Chlorotic Mottle Virus. [20] Many systematic investigations of this self-assembly process have been carried out using CP from cowpea chlorotic mottle virus, with a wide range of sequences and lengths of single-stranded RNA. [21] Virus like particles obtained from the Cowpea Chlorotic Mottle Virus (CCMV) represent an innovative platform for drug delivery applications. [22]在可用于实现核酸递送的纳米载体中，从豇豆褪绿斑驳病毒 (CCMV) 获得的病毒样颗粒 (VLP) 是一个吸引人的平台，因为它们具有负载能力、易于制造和功能化的顺应性。 [1] 本研究为解决VLPs表达低、组装效率低的问题，对8个具有代表性的VLPs（乙肝病毒核心抗原蛋白颗粒、Qbeta噬菌体、MS2噬菌体、P22噬菌体、豇豆）的组装纯化条件进行了研究。优化了在大肠杆菌中表达的褪绿斑驳病毒、烟草花叶病毒、铁蛋白和包膜蛋白）。 [2] PVENVs 已经使用几种植物病毒开发，例如豇豆褪绿斑驳病毒、马铃薯 X 病毒、豇豆花叶病毒、烟草花叶病毒和雀麦花叶病毒；这些植物病毒被广泛用于设计与多种生物相容性分子结合的纳米材料。 [3] 为此，我们通过结合蛋白质工程和生物正交化学，研究了豇豆褪绿斑驳病毒 (CCMV) 衣壳的功能化能力，该衣壳在内部用稳定的弹性蛋白样多肽 (ELP) 标签进行了修饰。 [4] 在这里，我们研究了每个纳米颗粒的标记数量如何影响荧光标记的豇豆褪绿斑驳病毒 (CCMV) 的总亮度。 [5] 我们之前通过测量其电泳迁移率研究了无包膜植物病毒豇豆褪绿斑驳病毒 (CCMV) 的电荷。 [6] 在检测到的所有病毒中，只有两种病毒，黄瓜花叶病毒和一种与豇豆褪绿斑驳病毒和雀麦花叶病毒有关的新型溴病毒，从野生植物机械传播到普通豆类植物。 [7] 使用模型病毒豇豆褪绿斑驳病毒并将其掺入猪精液样本中。 [8] 本文制备了用豇豆褪绿斑驳病毒衣壳蛋白 (ND-CP) 封装的高度稳定的 ND。 [9] 为了克服缺乏适当的内部和外部阳性对照以及检测 RNA 的不稳定性，我们使用噬菌体 Qβ 和植物病毒豇豆褪绿斑驳病毒 (CCMV) 开发了病毒样颗粒 (VLP)，用于包裹目标 RNA，即所谓的 SARS-CoV-2 LAMP 检测模块 (SLDM)。 [10] 肝素特异性结合肽与豇豆褪绿斑驳病毒 (CCMV) 衣壳蛋白结合，随后允许其包裹肝素并形成衣壳样蛋白笼。 [11] 源自植物溴病毒属的 VLP，特别是豇豆褪绿斑驳病毒 (CCMV)，是 T=3 的二十面体颗粒。 [12] 特别是，源自感染植物的病毒的 VLP，如豇豆褪绿斑驳病毒 (CCMV)，其中衣壳蛋白 (CP) 包裹 563 bp 的 dsRNA，显示可沉默 WSSV 糖蛋白 VP28 (dsRNAvp28)。 [13] 因此，我们使用等温滴定量热法研究了以聚阴离子物种（货物）为模板的豇豆褪绿斑驳病毒（CCMV）的CP，即单链DNA（ssDNA）和聚苯乙烯磺酸盐（PSS）的组装。 [14] 我们开发了一个利用自组装豇豆褪绿斑驳病毒 (CCMV) 的植物病毒 siRNA 递送平台。 [15] 其他新兴的植物病毒推断系统包括通常等距的豇豆褪绿斑驳病毒 (CCMV)，其结构进一步显着改变，直至带有突出核酸的“樱桃炸弹”。 [16] 这些纳米反应器是通过将各种不同的酶封装在豇豆褪绿斑驳病毒 (CCMV) 的蛋白衣壳中制成的。 [17] 在这里，我们报告了 DNA 周围豇豆褪绿斑驳病毒 (CCMV) 的衣壳蛋白在中性 pH 值下组装成确定的结构。 [18] 从植物病毒豇豆褪绿斑驳病毒 (CCMV) 中纯化的衣壳蛋白用于在体外组装含有报告蛋白（例如。 [19] 在这里，我们研究了点突变对先前预测的二十面体豇豆褪绿斑驳病毒热点的影响。 [20] 已经使用来自豇豆褪绿斑驳病毒的 CP 对这种自组装过程进行了许多系统研究，该病毒具有广泛的序列和长度的单链 RNA。 [21] 从豇豆褪绿斑驳病毒 (CCMV) 中获得的病毒样颗粒代表了药物输送应用的创新平台。 [22]
Viru Cowpea Chlorotic 豇豆病毒
We have previously investigated the charge on the unenveloped plant virus Cowpea Chlorotic Mottle Virus (CCMV) by measurements of its electrophoretic mobility. [1] To overcome the lack of proper internal and external positive controls and the instability of the detection RNA, we developed virus-like particles (VLPs) using bacteriophage Qβ and plant virus cowpea chlorotic mottle virus (CCMV) for the encapsidation of target RNA, namely a so-called SARS-CoV-2 LAMP detection module (SLDM). [2] Purified capsid protein from the plant virus Cowpea Chlorotic Mottle Virus (CCMV) is used to in vitro assemble monodisperse virus-like particles (VLPs) containing reporter proteins (e. [3]我们之前通过测量其电泳迁移率研究了无包膜植物病毒豇豆褪绿斑驳病毒 (CCMV) 的电荷。 [1] 为了克服缺乏适当的内部和外部阳性对照以及检测 RNA 的不稳定性，我们使用噬菌体 Qβ 和植物病毒豇豆褪绿斑驳病毒 (CCMV) 开发了病毒样颗粒 (VLP)，用于包裹目标 RNA，即所谓的 SARS-CoV-2 LAMP 检测模块 (SLDM)。 [2] 从植物病毒豇豆褪绿斑驳病毒 (CCMV) 中纯化的衣壳蛋白用于在体外组装含有报告蛋白（例如。 [3]
cowpea chlorotic mottle 豇豆褪绿斑驳
Among nanocarriers that can be used to achieve nucleic acid delivery, virus-like particles (VLPs) obtained from the Cowpea chlorotic mottle virus (CCMV) are an appealing platform, because of their loading capacity, ease of manufacture and amenability for functionalization. [1] In this study, in order to solve the problem of low expression and low assembly efficiency of VLPs, the conditions for the assembly and purification of eight representative VLPs (hepatitis B virus core antigen protein particles, Qbeta phage, MS2 phage, P22 phage, cowpea chlorotic mottle virus, tobacco Mosaic virus, ferritin and encapsulin) expressed in Escherichia coli were optimized. [2] PVENVs have been developed using several plant viruses, such as cowpea chlorotic mottle virus, potato virus X, cowpea mosaic virus, tobacco mosaic virus, and Brome mosaic virus; these plant viruses are widely used to engineer nanomaterials conjugated with a wide range of biocompatible molecules. [3] For this purpose, we investigated the functionalization capacity of the capsid of cowpea chlorotic mottle virus (CCMV), modified at the interior with a stabilizing elastin-like polypeptide (ELP) tag, by employing a combination of protein engineering and bio-orthogonal chemistry. [4] Here we study how the number of labels per nanoparticle influences the total brightness of fluorescently labelled cowpea chlorotic mottle virus (CCMV). [5] We have previously investigated the charge on the unenveloped plant virus Cowpea Chlorotic Mottle Virus (CCMV) by measurements of its electrophoretic mobility. [6] Of all viruses detected, only two viruses, cucumber mosaic virus and a novel bromovirus related to cowpea chlorotic mottle virus and brome mosaic virus, were mechanically transmitted from wild plants to common bean plants. [7] A model virus, cowpea chlorotic mottle virus, was used and spiked to porcine semen samples. [8] Herein, highly stable NDs encapsulated with Cowpea chlorotic mottle virus capsid proteins (ND-CP) are prepared. [9] To overcome the lack of proper internal and external positive controls and the instability of the detection RNA, we developed virus-like particles (VLPs) using bacteriophage Qβ and plant virus cowpea chlorotic mottle virus (CCMV) for the encapsidation of target RNA, namely a so-called SARS-CoV-2 LAMP detection module (SLDM). [10] A heparin-specific binding peptide was conjugated to a cowpea chlorotic mottle virus (CCMV) capsid protein, which was subsequently allowed to encapsulate heparin and form capsid-like protein cages. [11] VLPs derived from the plant Bromovirus genus, specifically cowpea chlorotic mottle virus (CCMV), are T = 3 icosahedral particles. [12] In particular, VLPs derived from a virus that infects plants, such as cowpea chlorotic mottle virus (CCMV), in which the capsid protein (CP) encapsidates the dsRNA of 563 bp, are shown to silence the WSSV glycoprotein VP28 (dsRNAvp28). [13] Therefore, we studied the assembly of CPs of the cowpea chlorotic mottle virus (CCMV) templated by polyanionic species (cargo), that is, single-stranded DNA (ssDNA), and polystyrene sulfonate (PSS) using isothermal titration calorimetry. [14] We developed a plant viral siRNA delivery platform making use of self-assembling cowpea chlorotic mottle virus (CCMV). [15] Other emerging plant virus-deduced systems include the usually isometric cowpea chlorotic mottle virus (CCMV) with further strikingly altered structures up to "cherrybombs" with protruding nucleic acids. [16] These nanoreactors are made by encapsulating various different enzymes in the protein capsid of the cowpea chlorotic mottle virus (CCMV). [17] Here we report the assembly of capsid proteins of the cowpea chlorotic mottle virus (CCMV) around DNA into defined structures at neutral pH. [18] Purified capsid protein from the plant virus Cowpea Chlorotic Mottle Virus (CCMV) is used to in vitro assemble monodisperse virus-like particles (VLPs) containing reporter proteins (e. [19] Here, we investigate the effect of point-mutations on previously predicted hot-spots of the icosahedral Cowpea Chlorotic Mottle Virus. [20] Many systematic investigations of this self-assembly process have been carried out using CP from cowpea chlorotic mottle virus, with a wide range of sequences and lengths of single-stranded RNA. [21] Virus like particles obtained from the Cowpea Chlorotic Mottle Virus (CCMV) represent an innovative platform for drug delivery applications. [22]在可用于实现核酸递送的纳米载体中，从豇豆褪绿斑驳病毒 (CCMV) 获得的病毒样颗粒 (VLP) 是一个吸引人的平台，因为它们具有负载能力、易于制造和功能化的顺应性。 [1] 本研究为解决VLPs表达低、组装效率低的问题，对8个具有代表性的VLPs（乙肝病毒核心抗原蛋白颗粒、Qbeta噬菌体、MS2噬菌体、P22噬菌体、豇豆）的组装纯化条件进行了研究。优化了在大肠杆菌中表达的褪绿斑驳病毒、烟草花叶病毒、铁蛋白和包膜蛋白）。 [2] PVENVs 已经使用几种植物病毒开发，例如豇豆褪绿斑驳病毒、马铃薯 X 病毒、豇豆花叶病毒、烟草花叶病毒和雀麦花叶病毒；这些植物病毒被广泛用于设计与多种生物相容性分子结合的纳米材料。 [3] 为此，我们通过结合蛋白质工程和生物正交化学，研究了豇豆褪绿斑驳病毒 (CCMV) 衣壳的功能化能力，该衣壳在内部用稳定的弹性蛋白样多肽 (ELP) 标签进行了修饰。 [4] 在这里，我们研究了每个纳米颗粒的标记数量如何影响荧光标记的豇豆褪绿斑驳病毒 (CCMV) 的总亮度。 [5] 我们之前通过测量其电泳迁移率研究了无包膜植物病毒豇豆褪绿斑驳病毒 (CCMV) 的电荷。 [6] 在检测到的所有病毒中，只有两种病毒，黄瓜花叶病毒和一种与豇豆褪绿斑驳病毒和雀麦花叶病毒有关的新型溴病毒，从野生植物机械传播到普通豆类植物。 [7] 使用模型病毒豇豆褪绿斑驳病毒并将其掺入猪精液样本中。 [8] 本文制备了用豇豆褪绿斑驳病毒衣壳蛋白 (ND-CP) 封装的高度稳定的 ND。 [9] 为了克服缺乏适当的内部和外部阳性对照以及检测 RNA 的不稳定性，我们使用噬菌体 Qβ 和植物病毒豇豆褪绿斑驳病毒 (CCMV) 开发了病毒样颗粒 (VLP)，用于包裹目标 RNA，即所谓的 SARS-CoV-2 LAMP 检测模块 (SLDM)。 [10] 肝素特异性结合肽与豇豆褪绿斑驳病毒 (CCMV) 衣壳蛋白结合，随后允许其包裹肝素并形成衣壳样蛋白笼。 [11] 源自植物溴病毒属的 VLP，特别是豇豆褪绿斑驳病毒 (CCMV)，是 T=3 的二十面体颗粒。 [12] 特别是，源自感染植物的病毒的 VLP，如豇豆褪绿斑驳病毒 (CCMV)，其中衣壳蛋白 (CP) 包裹 563 bp 的 dsRNA，显示可沉默 WSSV 糖蛋白 VP28 (dsRNAvp28)。 [13] 因此，我们使用等温滴定量热法研究了以聚阴离子物种（货物）为模板的豇豆褪绿斑驳病毒（CCMV）的CP，即单链DNA（ssDNA）和聚苯乙烯磺酸盐（PSS）的组装。 [14] 我们开发了一个利用自组装豇豆褪绿斑驳病毒 (CCMV) 的植物病毒 siRNA 递送平台。 [15] 其他新兴的植物病毒推断系统包括通常等距的豇豆褪绿斑驳病毒 (CCMV)，其结构进一步显着改变，直至带有突出核酸的“樱桃炸弹”。 [16] 这些纳米反应器是通过将各种不同的酶封装在豇豆褪绿斑驳病毒 (CCMV) 的蛋白衣壳中制成的。 [17] 在这里，我们报告了 DNA 周围豇豆褪绿斑驳病毒 (CCMV) 的衣壳蛋白在中性 pH 值下组装成确定的结构。 [18] 从植物病毒豇豆褪绿斑驳病毒 (CCMV) 中纯化的衣壳蛋白用于在体外组装含有报告蛋白（例如。 [19] 在这里，我们研究了点突变对先前预测的二十面体豇豆褪绿斑驳病毒热点的影响。 [20] 已经使用来自豇豆褪绿斑驳病毒的 CP 对这种自组装过程进行了许多系统研究，该病毒具有广泛的序列和长度的单链 RNA。 [21] 从豇豆褪绿斑驳病毒 (CCMV) 中获得的病毒样颗粒代表了药物输送应用的创新平台。 [22]