## Bladder Acellular(膀胱无细胞)研究综述

Bladder Acellular 膀胱无细胞 - Bladder acellular matrix (BAM) with inherent bioactive factors, a natural extracellular matrix (ECM) derived biomaterial, has been widely used as a scaffold to facilitate the repair and reconstruction of urinary tissues.^{[1]}In order to address the disadvantage of rapid degradation and serious immune response of bladder acellular matrix (BAM) tissues in clinical application, in this study, oxidized carboxymethyl cellulose (DCMC) was developed to replace glutaraldehyde (GA), a most common synthetic crosslinking reagent in clinical practice, to fix BAM tissues for lower cytotoxicity.

^{[2]}In this study, adipose tissue-derived SVFs were introduced as an angiogenic cell source and seeded into the bladder acellular matrix (BAM) to generate a SVF-BAM complex for bladder reconstruction.

^{[3]}Consequently, inspired from the native urethra, bacterial cellulose (BC) and bladder acellular matrix (BAM) were combined to design a three dimensional (3D) biomimetic scaffold.

^{[4]}Rats were randomly divided into the following four groups: hp-ADEPC, ADEPC, bladder acellular matrix (BAM), and cystotomy groups.

^{[5]}We have pioneered the development of a Tissue Engineered Muscle Repair (TEMR) technology created by seeding muscle progenitor cells (MPCs) onto a porcine derived bladder acellular matrix (BAM) followed by cyclic stretch preconditioning prior to implantation.

^{[6]}MethodsRat urinary bladders were reconstructed with bladder acellular matrix (BAM) (n = 52) or BAM seeded with adipose tissue-derived mesenchymal stromal cells (ASCs) (n = 52).

^{[7]}

具有固有生物活性因子的膀胱脱细胞基质（BAM）是一种天然的细胞外基质（ECM）衍生的生物材料，已被广泛用作促进泌尿组织修复和重建的支架。

^{[1]}为了解决膀胱脱细胞基质（BAM）组织在临床应用中降解快、免疫反应严重的缺点，本研究开发了氧化羧甲基纤维素（DCMC）来替代最常见的合成交联剂戊二醛（GA）。在临床实践中，固定 BAM 组织以降低细胞毒性。

^{[2]}在这项研究中，脂肪组织衍生的 SVF 作为血管生成细胞源被引入并接种到膀胱脱细胞基质 (BAM) 中，以生成用于膀胱重建的 SVF-BAM 复合物。

^{[3]}因此，受天然尿道的启发，将细菌纤维素 (BC) 和膀胱脱细胞基质 (BAM) 结合起来设计了一个三维 (3D) 仿生支架。

^{[4]}将大鼠随机分为以下四组：hp-ADEPC、ADEPC、膀胱无细胞基质（BAM）和膀胱切开术组。

^{[5]}我们率先开发了组织工程肌肉修复 (TEMR) 技术，该技术通过将肌肉祖细胞 (MPC) 接种到猪衍生的膀胱脱细胞基质 (BAM) 上，然后在植入前进行循环拉伸预处理。

^{[6]}方法用膀胱脱细胞基质（BAM）（n = 52）或接种脂肪组织来源的间充质基质细胞（ASCs）的BAM（n = 52）重建大鼠膀胱。

^{[7]}

## bladder acellular matrix 膀胱脱细胞基质

Bladder acellular matrix (BAM) with inherent bioactive factors, a natural extracellular matrix (ECM) derived biomaterial, has been widely used as a scaffold to facilitate the repair and reconstruction of urinary tissues.^{[1]}In order to address the disadvantage of rapid degradation and serious immune response of bladder acellular matrix (BAM) tissues in clinical application, in this study, oxidized carboxymethyl cellulose (DCMC) was developed to replace glutaraldehyde (GA), a most common synthetic crosslinking reagent in clinical practice, to fix BAM tissues for lower cytotoxicity.

^{[2]}In this study, adipose tissue-derived SVFs were introduced as an angiogenic cell source and seeded into the bladder acellular matrix (BAM) to generate a SVF-BAM complex for bladder reconstruction.

^{[3]}Consequently, inspired from the native urethra, bacterial cellulose (BC) and bladder acellular matrix (BAM) were combined to design a three dimensional (3D) biomimetic scaffold.

^{[4]}Rats were randomly divided into the following four groups: hp-ADEPC, ADEPC, bladder acellular matrix (BAM), and cystotomy groups.

^{[5]}We have pioneered the development of a Tissue Engineered Muscle Repair (TEMR) technology created by seeding muscle progenitor cells (MPCs) onto a porcine derived bladder acellular matrix (BAM) followed by cyclic stretch preconditioning prior to implantation.

^{[6]}MethodsRat urinary bladders were reconstructed with bladder acellular matrix (BAM) (n = 52) or BAM seeded with adipose tissue-derived mesenchymal stromal cells (ASCs) (n = 52).

^{[7]}

具有固有生物活性因子的膀胱脱细胞基质（BAM）是一种天然的细胞外基质（ECM）衍生的生物材料，已被广泛用作促进泌尿组织修复和重建的支架。

^{[1]}为了解决膀胱脱细胞基质（BAM）组织在临床应用中降解快、免疫反应严重的缺点，本研究开发了氧化羧甲基纤维素（DCMC）来替代最常见的合成交联剂戊二醛（GA）。在临床实践中，固定 BAM 组织以降低细胞毒性。

^{[2]}在这项研究中，脂肪组织衍生的 SVF 作为血管生成细胞源被引入并接种到膀胱脱细胞基质 (BAM) 中，以生成用于膀胱重建的 SVF-BAM 复合物。

^{[3]}因此，受天然尿道的启发，将细菌纤维素 (BC) 和膀胱脱细胞基质 (BAM) 结合起来设计了一个三维 (3D) 仿生支架。

^{[4]}将大鼠随机分为以下四组：hp-ADEPC、ADEPC、膀胱无细胞基质（BAM）和膀胱切开术组。

^{[5]}我们率先开发了组织工程肌肉修复 (TEMR) 技术，该技术通过将肌肉祖细胞 (MPC) 接种到猪衍生的膀胱脱细胞基质 (BAM) 上，然后在植入前进行循环拉伸预处理。

^{[6]}方法用膀胱脱细胞基质（BAM）（n = 52）或接种脂肪组织来源的间充质基质细胞（ASCs）的BAM（n = 52）重建大鼠膀胱。

^{[7]}