Apple Chlorotic(苹果变色)研究综述
Apple Chlorotic 苹果变色 - , 2015) and subjected to reverse transcription (RT)-PCR to detect apple chlorotic leaf spot virus (ACLSV), apple stem pitting virus (ASPV), apple stem grooving virus (ASGV), apple necrotic mosaic virus (ApNMV), apple scar skin viroid (ASSVd), ARWV-2, ARWV-1, ALV, and CiVA, using primers specific to respective viruses (Supplementary Table 1). [1] A total of 98 samples collected from 48 apple cultivars were assessed to detect the occurrence and prevalence of lethal viruses such as Apple stem pitting virus, Apple chlorotic leaf spot virus, Apple stem grooving virus and Tomato ring spot virus. [2] Apple chlorotic leaf spot virus (ACLSV) is an important virus infecting fruit trees. [3] The prevalence of harmful viruses of Apple stem grooving virus (ASGV), Apple stem pitting virus (ASPV), Apple chlorotic leaf spot virus (ACLSV), Apple mosaic virus (ApMV), depending on the location, age, and varietal composition of plantings, was studied. [4] Over the course of developing and applying a new real-time PCR assay for the detection of the newly described apple chlorotic fruit spot viroid (ACFSVd), slight modifications of the reverse transcription (RT) step were found to improve significantly the detection limit of the assay. [5] The 5 apple viruses, apple chlorotic leaf spot virus (ACLSV), apple stem grooving virus (ASGV), apple stem pitting virus (ASPV), apple mosaic virus (ApMV) and prunus necrotic ring spot virus (PNRSV) and 1 viroid, apple scar skin viroid (ASSVd) are known major viral/viroid pathogens of apple in India. [6] In this study, the apple cultivars ‘Ruiyang’, ‘Ruixue’, ‘Rui Xianghong’ and ‘Qinyue’ were used as source materials to eradicate the latent viruses (Apple chlorotic leaf spot virus, ACLSV; Apple stem grooving virus, ASGV; Apple stem pitting virus, ASPV), investigated the different virus eradication effects of combining thermotherapy with shoot tip culture (STC) or cryotherapy. [7] The specificity assay shows no amplification in major peach viruses, apple chlorotic leaf spot virus and prunus necrotic ring spot virus and negative control. [8] The following agents were reported in Uruguay in stone fruit trees: PNRSV (Prunus necrotic ringspot virus), PDV (Prune dwarf virus), ACLSV (Apple chlorotic leafspot virus), ApMV (Apple mosaic virus) and PLMVd (Peach latent mosaic viroid). [9] RT-PCR and high-throughput sequencing (HTS) of symptomatic loquat plants led to first-time detection and characterization of apple stem grooving virus (ASGV), also known as citrus tatter leaf virus (CTLV), and apple chlorotic leaf spot virus (ACLSV) from Spain with description of nearly complete genomic sequences. [10] Virus or viroid infection including apple chlorotic leaf spot virus (ACLSV), apple stem pitting virus (ASPV), apple stem grooving virus (ASGV), apple mosaic virus (ApMV) and apple scar skin viroid (ASSVd) have been also reported in Korea. [11] Stone fruits are an important crop in most parts of the world and are heavily challenged by several viruses including Plum pox virus (PPV), Prune dwarf virus (PDV), Prunus necrotic ringspot virus (PNRSV), and Apple chlorotic leaf spot virus (ACLSV). [12] This study evaluated the effectiveness of a droplet-vitrification cryopreservation technique in eradicating latent viruses: Apple chlorotic leaf spot virus (ACLSV), Apple stem grooving virus (ASGV) and Apple stem pitting virus (ASPV) from in vitro axillary shoot tips excised from ‘SC417 Monalisa’ apple cultivar shoot tips. [13] Prunus necrotic ring spot ilarvirus (PNRSV) and Apple chlorotic leaf spot trichovirus (ACLSV) are common in plum orchards. [14] The high-throughput RNA sequencing generated contigs derived from two recently discovered viruses (apple luteovirus 1 [ALV-1] and apple rubbery wood virus 1 [ARWV-1]) not reported in Korea, as well as five viruses (apple chlorotic leaf spot virus, apple green crinkle associated virus, apple stem grooving virus, apple stem pitting virus, and apricot latent virus [Cho et al. [15] We attempted to develop a deconstructed vector based on the genome of grapevine virus A (GVA) by replacing the coat protein gene of GVA to the coat protein gene (aCP) of ACLSV (apple chlorotic leaf spot virus). [16] Illumina RNA-sequencing of double-stranded RNAs from leaves of diseased cherry trees (S1, S2, S3, and S4) indicated that all trees were infected with at least six known viruses, LChV-1, LChV-2, CNRMV, CGRMV, CVA, prune dwarf virus (PDV), and/or apple chlorotic leaf spot virus. [17] Based on our results and the demarcation criteria for viroids, the tentatively named “apple chlorotic fruit spot viroid” should be considered a putative new member of the genus Apscaviroid. [18] These three apple varieties also harbored apple chlorotic leaf spot virus (ACLSV), apple stem grooving virus (ASGV), and apple stem pitting virus (ASPV). [19] Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV), Apple stem grooving virus (ASGV), and Apple scar skin viroid (ASSVd) are economically important viruses that infect pear tree species worldwide. [20] Samples were tested for the presence of apple chlorotic leaf spot virus (ACLSV), apple stem pitting virus (ASPV), apple stem grooving virus (ASGV), and apple mosaic virus (ApMV). [21] A highly sensitive nested multiplex reverse transcription-polymerase chain reaction (nmRT-PCR) assay was developed for the simultaneous detection of Apple chlorotic leaf spot virus (ACLSV), Apple stem grooving virus (ASGV) and Apple stem pitting virus (ASPV) infecting pear trees. [22] Apple chlorotic leaf spot virus (ACLSV), also known as apricot pseudo pox, “viruela” (in Spain) and “butteratura” (in Italy), has had a significant impact on the production of Prunus species, including apricot (P. [23] Apple chlorotic leaf spot virus (ACLSV) is one of the most common viruses infecting apple and pear trees, and Iran is among the top ten apple-producing countries in the world. [24] Ninety sweet and sour cherry leaf samples showing suspicious virus symptoms were collected and screened with virus-specific primers: Little cherry virus 1 (LChV1), Cherry necrotic rusty mottle virus (CNRMV), Prune dwarf virus (PDV), Prune necrotic ring spot virus (PNRSV), Apple mosaic virus (ApMV), Cherry green ring mottle virus (CGRMV), Cherry leaf roll virus (CLRV), Cherry mottle leaf virus (CMLV), Plum bark necrotic stem pitting associated virus (PBNSPaV), Cherry twisted leaf virus (CTLV), Apple stem grooving virus (ASGV), Little cherry virus 2 (LChV2), Cherry rusty leaf virus (CRLV), Apple stem pitting virus (ASPV), Apple chlorotic leaf spot virus (ACLSV). [25], 2015) 并通过逆转录 (RT)-PCR 检测苹果褪绿叶斑病毒 (ACLSV)、苹果茎点蚀病毒 (ASPV)、苹果茎沟病毒 (ASGV)、苹果坏死花叶病毒 (ApNMV)、苹果疤痕皮肤类病毒 (ASSVd)、ARWV-2、ARWV-1、ALV 和 CiVA，使用特定于各自病毒的引物（补充表 1）。 [1] 对从48个苹果品种采集的98份样本进行了评估，以检测苹果茎坑病毒、苹果褪绿叶斑病毒、苹果茎沟病毒和番茄环斑病毒等致死病毒的发生和流行情况。 [2] 苹果褪绿叶斑病毒（ACLSV）是一种感染果树的重要病毒。 [3] 苹果茎沟病毒（ASGV）、苹果茎点蚀病毒（ASPV）、苹果褪绿叶斑病毒（ACLSV）、苹果花叶病毒（ApMV）等有害病毒的流行率，取决于种植地点、年龄和品种组成, 进行了研究。 [4] nan [5] 5种苹果病毒，苹果褪绿叶斑病毒（ACLSV）、苹果茎沟病毒（ASGV）、苹果茎点蚀病毒（ASPV）、苹果花叶病毒（ApMV）和李坏环斑病毒（PNRSV）和1个类病毒，苹果疤痕皮肤类病毒 (ASSVd) 是印度已知的苹果主要病毒/类病毒病原体。 [6] nan [7] 特异性测定显示在主要桃病毒、苹果褪绿叶斑病毒和李坏环斑病毒和阴性对照中没有扩增。 [8] 乌拉圭在核果树中报告了以下病原体：PNRSV（李属坏死环斑病毒）、PDV（李属矮化病毒）、ACLSV（苹果褪绿叶斑病毒）、ApMV（苹果花叶病毒）和PLMVd（桃潜伏花叶病毒）。 [9] nan [10] 韩国也报告了病毒或类病毒感染，包括苹果褪绿叶斑病毒（ACLSV）、苹果茎点蚀病毒（ASPV）、苹果茎沟病毒（ASGV）、苹果花叶病毒（ApMV）和苹果疤痕皮肤病毒（ASSVd） . [11] 核果是世界大部分地区的重要作物，受到多种病毒的严重挑战，包括李痘病毒 (PPV)、李子矮化病毒 (PDV)、李子坏死环斑病毒 (PNRSV) 和苹果褪绿叶斑病毒 (ACLSV) ）。 [12] 这项研究评估了液滴玻璃化冷冻保存技术在根除潜伏病毒方面的有效性：从 ' SC417 蒙娜丽莎苹果品种的枝条提示。 [13] 李子坏死环斑病毒 (PNRSV) 和苹果褪绿叶斑毛滴虫病毒 (ACLSV) 在李园中很常见。 [14] 高通量 RNA 测序产生的 contigs 来源于最近发现的两种病毒（苹果黄体病毒 1 [ALV-1] 和苹果橡胶木病毒 1 [ARWV-1]）以及五种病毒（苹果褪绿叶斑病）病毒、苹果青皱相关病毒、苹果茎沟病毒、苹果茎点蚀病毒和杏潜伏病毒 [Cho et al. [15] 我们试图通过将 GVA 的外壳蛋白基因替换为 ACLSV（苹果褪绿叶斑病毒）的外壳蛋白基因（aCP）来开发基于葡萄病毒 A（GVA）基因组的解构载体。 [16] 来自患病樱桃树（S1、S2、S3 和 S4）叶子的双链 RNA 的 Illumina RNA 测序表明，所有树木都感染了至少六种已知病毒，LChV-1、LChV-2、CNRMV、CGRMV、 CVA、修剪矮化病毒 (PDV) 和/或苹果褪绿叶斑病毒。 [17] 根据我们的研究结果和类病毒的划分标准，暂定名为“苹果褪绿果斑类病毒”应该被认为是拟拟病毒属的新成员。 [18] 这三个苹果品种还含有苹果褪绿叶斑病毒（ACLSV）、苹果茎沟病毒（ASGV）和苹果茎点蚀病毒（ASPV）。 [19] 苹果褪绿叶斑病毒 (ACLSV)、苹果茎点蚀病毒 (ASPV)、苹果茎沟病毒 (ASGV) 和苹果疤痕皮肤病毒 (ASSVd) 是在全球范围内感染梨树种的重要经济病毒。 [20] 检测样品中是否存在苹果褪绿叶斑病毒 (ACLSV)、苹果茎点蚀病毒 (ASPV)、苹果茎沟病毒 (ASGV) 和苹果花叶病毒 (ApMV)。 [21] 开发了一种高度灵敏的嵌套多重逆转录聚合酶链反应 (nmRT-PCR) 测定法，用于同时检测感染梨的苹果褪绿叶斑病毒 (ACLSV)、苹果茎沟病毒 (ASGV) 和苹果茎点蚀病毒 (ASPV)树木。 [22] 苹果褪绿叶斑病毒（ACLSV），也称为杏假痘、“viruela”（在西班牙）和“butteratura”（在意大利），对包括杏（P. [23] 苹果褪绿叶斑病毒（ACLSV）是感染苹果和梨树最常见的病毒之一，伊朗是世界十大苹果生产国之一。 [24] 收集了 90 个显示可疑病毒症状的糖醋樱桃叶样本，并使用病毒特异性引物进行筛选：小樱桃病毒 1 (LChV1)、樱桃坏死锈斑病毒 (CNRMV)、李子矮化病毒 (PDV)、李子坏死环斑病毒(PNRSV)、苹果花叶病毒 (ApMV)、樱桃绿环斑驳病毒 (CGRMV)、樱桃卷叶病毒 (CLRV)、樱桃斑驳叶病毒 (CMLV)、李树皮坏死茎点蚀相关病毒 (PBNSPaV)、樱桃扭叶病毒病毒 (CTLV)、苹果茎沟病毒 (ASGV)、小樱桃病毒 2 (LChV2)、樱桃锈叶病毒 (CRLV)、苹果茎点蚀病毒 (ASPV)、苹果褪绿叶斑病毒 (ACLSV)。 [25]
apple stem grooving 苹果茎开槽
The prevalence of harmful viruses of Apple stem grooving virus (ASGV), Apple stem pitting virus (ASPV), Apple chlorotic leaf spot virus (ACLSV), Apple mosaic virus (ApMV), depending on the location, age, and varietal composition of plantings, was studied. [1] The 5 apple viruses, apple chlorotic leaf spot virus (ACLSV), apple stem grooving virus (ASGV), apple stem pitting virus (ASPV), apple mosaic virus (ApMV) and prunus necrotic ring spot virus (PNRSV) and 1 viroid, apple scar skin viroid (ASSVd) are known major viral/viroid pathogens of apple in India. [2] In this study, the apple cultivars ‘Ruiyang’, ‘Ruixue’, ‘Rui Xianghong’ and ‘Qinyue’ were used as source materials to eradicate the latent viruses (Apple chlorotic leaf spot virus, ACLSV; Apple stem grooving virus, ASGV; Apple stem pitting virus, ASPV), investigated the different virus eradication effects of combining thermotherapy with shoot tip culture (STC) or cryotherapy. [3] RT-PCR and high-throughput sequencing (HTS) of symptomatic loquat plants led to first-time detection and characterization of apple stem grooving virus (ASGV), also known as citrus tatter leaf virus (CTLV), and apple chlorotic leaf spot virus (ACLSV) from Spain with description of nearly complete genomic sequences. [4] This study evaluated the effectiveness of a droplet-vitrification cryopreservation technique in eradicating latent viruses: Apple chlorotic leaf spot virus (ACLSV), Apple stem grooving virus (ASGV) and Apple stem pitting virus (ASPV) from in vitro axillary shoot tips excised from ‘SC417 Monalisa’ apple cultivar shoot tips. [5] The high-throughput RNA sequencing generated contigs derived from two recently discovered viruses (apple luteovirus 1 [ALV-1] and apple rubbery wood virus 1 [ARWV-1]) not reported in Korea, as well as five viruses (apple chlorotic leaf spot virus, apple green crinkle associated virus, apple stem grooving virus, apple stem pitting virus, and apricot latent virus [Cho et al. [6] These three apple varieties also harbored apple chlorotic leaf spot virus (ACLSV), apple stem grooving virus (ASGV), and apple stem pitting virus (ASPV). [7] A highly sensitive nested multiplex reverse transcription-polymerase chain reaction (nmRT-PCR) assay was developed for the simultaneous detection of Apple chlorotic leaf spot virus (ACLSV), Apple stem grooving virus (ASGV) and Apple stem pitting virus (ASPV) infecting pear trees. [8] Ninety sweet and sour cherry leaf samples showing suspicious virus symptoms were collected and screened with virus-specific primers: Little cherry virus 1 (LChV1), Cherry necrotic rusty mottle virus (CNRMV), Prune dwarf virus (PDV), Prune necrotic ring spot virus (PNRSV), Apple mosaic virus (ApMV), Cherry green ring mottle virus (CGRMV), Cherry leaf roll virus (CLRV), Cherry mottle leaf virus (CMLV), Plum bark necrotic stem pitting associated virus (PBNSPaV), Cherry twisted leaf virus (CTLV), Apple stem grooving virus (ASGV), Little cherry virus 2 (LChV2), Cherry rusty leaf virus (CRLV), Apple stem pitting virus (ASPV), Apple chlorotic leaf spot virus (ACLSV). [9]苹果茎沟病毒（ASGV）、苹果茎点蚀病毒（ASPV）、苹果褪绿叶斑病毒（ACLSV）、苹果花叶病毒（ApMV）等有害病毒的流行率，取决于种植地点、年龄和品种组成, 进行了研究。 [1] 5种苹果病毒，苹果褪绿叶斑病毒（ACLSV）、苹果茎沟病毒（ASGV）、苹果茎点蚀病毒（ASPV）、苹果花叶病毒（ApMV）和李坏环斑病毒（PNRSV）和1个类病毒，苹果疤痕皮肤类病毒 (ASSVd) 是印度已知的苹果主要病毒/类病毒病原体。 [2] nan [3] nan [4] 这项研究评估了液滴玻璃化冷冻保存技术在根除潜伏病毒方面的有效性：从 ' SC417 蒙娜丽莎苹果品种的枝条提示。 [5] 高通量 RNA 测序产生的 contigs 来源于最近发现的两种病毒（苹果黄体病毒 1 [ALV-1] 和苹果橡胶木病毒 1 [ARWV-1]）以及五种病毒（苹果褪绿叶斑病）病毒、苹果青皱相关病毒、苹果茎沟病毒、苹果茎点蚀病毒和杏潜伏病毒 [Cho et al. [6] 这三个苹果品种还含有苹果褪绿叶斑病毒（ACLSV）、苹果茎沟病毒（ASGV）和苹果茎点蚀病毒（ASPV）。 [7] 开发了一种高度灵敏的嵌套多重逆转录聚合酶链反应 (nmRT-PCR) 测定法，用于同时检测感染梨的苹果褪绿叶斑病毒 (ACLSV)、苹果茎沟病毒 (ASGV) 和苹果茎点蚀病毒 (ASPV)树木。 [8] 收集了 90 个显示可疑病毒症状的糖醋樱桃叶样本，并使用病毒特异性引物进行筛选：小樱桃病毒 1 (LChV1)、樱桃坏死锈斑病毒 (CNRMV)、李子矮化病毒 (PDV)、李子坏死环斑病毒(PNRSV)、苹果花叶病毒 (ApMV)、樱桃绿环斑驳病毒 (CGRMV)、樱桃卷叶病毒 (CLRV)、樱桃斑驳叶病毒 (CMLV)、李树皮坏死茎点蚀相关病毒 (PBNSPaV)、樱桃扭叶病毒病毒 (CTLV)、苹果茎沟病毒 (ASGV)、小樱桃病毒 2 (LChV2)、樱桃锈叶病毒 (CRLV)、苹果茎点蚀病毒 (ASPV)、苹果褪绿叶斑病毒 (ACLSV)。 [9]
apple stem pitting 苹果茎点蚀
, 2015) and subjected to reverse transcription (RT)-PCR to detect apple chlorotic leaf spot virus (ACLSV), apple stem pitting virus (ASPV), apple stem grooving virus (ASGV), apple necrotic mosaic virus (ApNMV), apple scar skin viroid (ASSVd), ARWV-2, ARWV-1, ALV, and CiVA, using primers specific to respective viruses (Supplementary Table 1). [1] A total of 98 samples collected from 48 apple cultivars were assessed to detect the occurrence and prevalence of lethal viruses such as Apple stem pitting virus, Apple chlorotic leaf spot virus, Apple stem grooving virus and Tomato ring spot virus. [2] Virus or viroid infection including apple chlorotic leaf spot virus (ACLSV), apple stem pitting virus (ASPV), apple stem grooving virus (ASGV), apple mosaic virus (ApMV) and apple scar skin viroid (ASSVd) have been also reported in Korea. [3] Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV), Apple stem grooving virus (ASGV), and Apple scar skin viroid (ASSVd) are economically important viruses that infect pear tree species worldwide. [4] Samples were tested for the presence of apple chlorotic leaf spot virus (ACLSV), apple stem pitting virus (ASPV), apple stem grooving virus (ASGV), and apple mosaic virus (ApMV). [5], 2015) 并通过逆转录 (RT)-PCR 检测苹果褪绿叶斑病毒 (ACLSV)、苹果茎点蚀病毒 (ASPV)、苹果茎沟病毒 (ASGV)、苹果坏死花叶病毒 (ApNMV)、苹果疤痕皮肤类病毒 (ASSVd)、ARWV-2、ARWV-1、ALV 和 CiVA，使用特定于各自病毒的引物（补充表 1）。 [1] 对从48个苹果品种采集的98份样本进行了评估，以检测苹果茎坑病毒、苹果褪绿叶斑病毒、苹果茎沟病毒和番茄环斑病毒等致死病毒的发生和流行情况。 [2] 韩国也报告了病毒或类病毒感染，包括苹果褪绿叶斑病毒（ACLSV）、苹果茎点蚀病毒（ASPV）、苹果茎沟病毒（ASGV）、苹果花叶病毒（ApMV）和苹果疤痕皮肤病毒（ASSVd） . [3] 苹果褪绿叶斑病毒 (ACLSV)、苹果茎点蚀病毒 (ASPV)、苹果茎沟病毒 (ASGV) 和苹果疤痕皮肤病毒 (ASSVd) 是在全球范围内感染梨树种的重要经济病毒。 [4] 检测样品中是否存在苹果褪绿叶斑病毒 (ACLSV)、苹果茎点蚀病毒 (ASPV)、苹果茎沟病毒 (ASGV) 和苹果花叶病毒 (ApMV)。 [5]
necrotic ring spot 坏死环斑
The specificity assay shows no amplification in major peach viruses, apple chlorotic leaf spot virus and prunus necrotic ring spot virus and negative control. [1]特异性测定显示在主要桃病毒、苹果褪绿叶斑病毒和李坏环斑病毒和阴性对照中没有扩增。 [1]
apple chlorotic leaf 苹果褪绿叶
, 2015) and subjected to reverse transcription (RT)-PCR to detect apple chlorotic leaf spot virus (ACLSV), apple stem pitting virus (ASPV), apple stem grooving virus (ASGV), apple necrotic mosaic virus (ApNMV), apple scar skin viroid (ASSVd), ARWV-2, ARWV-1, ALV, and CiVA, using primers specific to respective viruses (Supplementary Table 1). [1] A total of 98 samples collected from 48 apple cultivars were assessed to detect the occurrence and prevalence of lethal viruses such as Apple stem pitting virus, Apple chlorotic leaf spot virus, Apple stem grooving virus and Tomato ring spot virus. [2] Apple chlorotic leaf spot virus (ACLSV) is an important virus infecting fruit trees. [3] The prevalence of harmful viruses of Apple stem grooving virus (ASGV), Apple stem pitting virus (ASPV), Apple chlorotic leaf spot virus (ACLSV), Apple mosaic virus (ApMV), depending on the location, age, and varietal composition of plantings, was studied. [4] The 5 apple viruses, apple chlorotic leaf spot virus (ACLSV), apple stem grooving virus (ASGV), apple stem pitting virus (ASPV), apple mosaic virus (ApMV) and prunus necrotic ring spot virus (PNRSV) and 1 viroid, apple scar skin viroid (ASSVd) are known major viral/viroid pathogens of apple in India. [5] In this study, the apple cultivars ‘Ruiyang’, ‘Ruixue’, ‘Rui Xianghong’ and ‘Qinyue’ were used as source materials to eradicate the latent viruses (Apple chlorotic leaf spot virus, ACLSV; Apple stem grooving virus, ASGV; Apple stem pitting virus, ASPV), investigated the different virus eradication effects of combining thermotherapy with shoot tip culture (STC) or cryotherapy. [6] The specificity assay shows no amplification in major peach viruses, apple chlorotic leaf spot virus and prunus necrotic ring spot virus and negative control. [7] RT-PCR and high-throughput sequencing (HTS) of symptomatic loquat plants led to first-time detection and characterization of apple stem grooving virus (ASGV), also known as citrus tatter leaf virus (CTLV), and apple chlorotic leaf spot virus (ACLSV) from Spain with description of nearly complete genomic sequences. [8] Virus or viroid infection including apple chlorotic leaf spot virus (ACLSV), apple stem pitting virus (ASPV), apple stem grooving virus (ASGV), apple mosaic virus (ApMV) and apple scar skin viroid (ASSVd) have been also reported in Korea. [9] Stone fruits are an important crop in most parts of the world and are heavily challenged by several viruses including Plum pox virus (PPV), Prune dwarf virus (PDV), Prunus necrotic ringspot virus (PNRSV), and Apple chlorotic leaf spot virus (ACLSV). [10] This study evaluated the effectiveness of a droplet-vitrification cryopreservation technique in eradicating latent viruses: Apple chlorotic leaf spot virus (ACLSV), Apple stem grooving virus (ASGV) and Apple stem pitting virus (ASPV) from in vitro axillary shoot tips excised from ‘SC417 Monalisa’ apple cultivar shoot tips. [11] Prunus necrotic ring spot ilarvirus (PNRSV) and Apple chlorotic leaf spot trichovirus (ACLSV) are common in plum orchards. [12] The high-throughput RNA sequencing generated contigs derived from two recently discovered viruses (apple luteovirus 1 [ALV-1] and apple rubbery wood virus 1 [ARWV-1]) not reported in Korea, as well as five viruses (apple chlorotic leaf spot virus, apple green crinkle associated virus, apple stem grooving virus, apple stem pitting virus, and apricot latent virus [Cho et al. [13] We attempted to develop a deconstructed vector based on the genome of grapevine virus A (GVA) by replacing the coat protein gene of GVA to the coat protein gene (aCP) of ACLSV (apple chlorotic leaf spot virus). [14] Illumina RNA-sequencing of double-stranded RNAs from leaves of diseased cherry trees (S1, S2, S3, and S4) indicated that all trees were infected with at least six known viruses, LChV-1, LChV-2, CNRMV, CGRMV, CVA, prune dwarf virus (PDV), and/or apple chlorotic leaf spot virus. [15] These three apple varieties also harbored apple chlorotic leaf spot virus (ACLSV), apple stem grooving virus (ASGV), and apple stem pitting virus (ASPV). [16] Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV), Apple stem grooving virus (ASGV), and Apple scar skin viroid (ASSVd) are economically important viruses that infect pear tree species worldwide. [17] Samples were tested for the presence of apple chlorotic leaf spot virus (ACLSV), apple stem pitting virus (ASPV), apple stem grooving virus (ASGV), and apple mosaic virus (ApMV). [18] A highly sensitive nested multiplex reverse transcription-polymerase chain reaction (nmRT-PCR) assay was developed for the simultaneous detection of Apple chlorotic leaf spot virus (ACLSV), Apple stem grooving virus (ASGV) and Apple stem pitting virus (ASPV) infecting pear trees. [19] Apple chlorotic leaf spot virus (ACLSV), also known as apricot pseudo pox, “viruela” (in Spain) and “butteratura” (in Italy), has had a significant impact on the production of Prunus species, including apricot (P. [20] Apple chlorotic leaf spot virus (ACLSV) is one of the most common viruses infecting apple and pear trees, and Iran is among the top ten apple-producing countries in the world. [21] Ninety sweet and sour cherry leaf samples showing suspicious virus symptoms were collected and screened with virus-specific primers: Little cherry virus 1 (LChV1), Cherry necrotic rusty mottle virus (CNRMV), Prune dwarf virus (PDV), Prune necrotic ring spot virus (PNRSV), Apple mosaic virus (ApMV), Cherry green ring mottle virus (CGRMV), Cherry leaf roll virus (CLRV), Cherry mottle leaf virus (CMLV), Plum bark necrotic stem pitting associated virus (PBNSPaV), Cherry twisted leaf virus (CTLV), Apple stem grooving virus (ASGV), Little cherry virus 2 (LChV2), Cherry rusty leaf virus (CRLV), Apple stem pitting virus (ASPV), Apple chlorotic leaf spot virus (ACLSV). [22], 2015) 并通过逆转录 (RT)-PCR 检测苹果褪绿叶斑病毒 (ACLSV)、苹果茎点蚀病毒 (ASPV)、苹果茎沟病毒 (ASGV)、苹果坏死花叶病毒 (ApNMV)、苹果疤痕皮肤类病毒 (ASSVd)、ARWV-2、ARWV-1、ALV 和 CiVA，使用特定于各自病毒的引物（补充表 1）。 [1] 对从48个苹果品种采集的98份样本进行了评估，以检测苹果茎坑病毒、苹果褪绿叶斑病毒、苹果茎沟病毒和番茄环斑病毒等致死病毒的发生和流行情况。 [2] 苹果褪绿叶斑病毒（ACLSV）是一种感染果树的重要病毒。 [3] 苹果茎沟病毒（ASGV）、苹果茎点蚀病毒（ASPV）、苹果褪绿叶斑病毒（ACLSV）、苹果花叶病毒（ApMV）等有害病毒的流行率，取决于种植地点、年龄和品种组成, 进行了研究。 [4] 5种苹果病毒，苹果褪绿叶斑病毒（ACLSV）、苹果茎沟病毒（ASGV）、苹果茎点蚀病毒（ASPV）、苹果花叶病毒（ApMV）和李坏环斑病毒（PNRSV）和1个类病毒，苹果疤痕皮肤类病毒 (ASSVd) 是印度已知的苹果主要病毒/类病毒病原体。 [5] nan [6] 特异性测定显示在主要桃病毒、苹果褪绿叶斑病毒和李坏环斑病毒和阴性对照中没有扩增。 [7] nan [8] 韩国也报告了病毒或类病毒感染，包括苹果褪绿叶斑病毒（ACLSV）、苹果茎点蚀病毒（ASPV）、苹果茎沟病毒（ASGV）、苹果花叶病毒（ApMV）和苹果疤痕皮肤病毒（ASSVd） . [9] 核果是世界大部分地区的重要作物，受到多种病毒的严重挑战，包括李痘病毒 (PPV)、李子矮化病毒 (PDV)、李子坏死环斑病毒 (PNRSV) 和苹果褪绿叶斑病毒 (ACLSV) ）。 [10] 这项研究评估了液滴玻璃化冷冻保存技术在根除潜伏病毒方面的有效性：从 ' SC417 蒙娜丽莎苹果品种的枝条提示。 [11] 李子坏死环斑病毒 (PNRSV) 和苹果褪绿叶斑毛滴虫病毒 (ACLSV) 在李园中很常见。 [12] 高通量 RNA 测序产生的 contigs 来源于最近发现的两种病毒（苹果黄体病毒 1 [ALV-1] 和苹果橡胶木病毒 1 [ARWV-1]）以及五种病毒（苹果褪绿叶斑病）病毒、苹果青皱相关病毒、苹果茎沟病毒、苹果茎点蚀病毒和杏潜伏病毒 [Cho et al. [13] 我们试图通过将 GVA 的外壳蛋白基因替换为 ACLSV（苹果褪绿叶斑病毒）的外壳蛋白基因（aCP）来开发基于葡萄病毒 A（GVA）基因组的解构载体。 [14] 来自患病樱桃树（S1、S2、S3 和 S4）叶子的双链 RNA 的 Illumina RNA 测序表明，所有树木都感染了至少六种已知病毒，LChV-1、LChV-2、CNRMV、CGRMV、 CVA、修剪矮化病毒 (PDV) 和/或苹果褪绿叶斑病毒。 [15] 这三个苹果品种还含有苹果褪绿叶斑病毒（ACLSV）、苹果茎沟病毒（ASGV）和苹果茎点蚀病毒（ASPV）。 [16] 苹果褪绿叶斑病毒 (ACLSV)、苹果茎点蚀病毒 (ASPV)、苹果茎沟病毒 (ASGV) 和苹果疤痕皮肤病毒 (ASSVd) 是在全球范围内感染梨树种的重要经济病毒。 [17] 检测样品中是否存在苹果褪绿叶斑病毒 (ACLSV)、苹果茎点蚀病毒 (ASPV)、苹果茎沟病毒 (ASGV) 和苹果花叶病毒 (ApMV)。 [18] 开发了一种高度灵敏的嵌套多重逆转录聚合酶链反应 (nmRT-PCR) 测定法，用于同时检测感染梨的苹果褪绿叶斑病毒 (ACLSV)、苹果茎沟病毒 (ASGV) 和苹果茎点蚀病毒 (ASPV)树木。 [19] 苹果褪绿叶斑病毒（ACLSV），也称为杏假痘、“viruela”（在西班牙）和“butteratura”（在意大利），对包括杏（P. [20] 苹果褪绿叶斑病毒（ACLSV）是感染苹果和梨树最常见的病毒之一，伊朗是世界十大苹果生产国之一。 [21] 收集了 90 个显示可疑病毒症状的糖醋樱桃叶样本，并使用病毒特异性引物进行筛选：小樱桃病毒 1 (LChV1)、樱桃坏死锈斑病毒 (CNRMV)、李子矮化病毒 (PDV)、李子坏死环斑病毒(PNRSV)、苹果花叶病毒 (ApMV)、樱桃绿环斑驳病毒 (CGRMV)、樱桃卷叶病毒 (CLRV)、樱桃斑驳叶病毒 (CMLV)、李树皮坏死茎点蚀相关病毒 (PBNSPaV)、樱桃扭叶病毒病毒 (CTLV)、苹果茎沟病毒 (ASGV)、小樱桃病毒 2 (LChV2)、樱桃锈叶病毒 (CRLV)、苹果茎点蚀病毒 (ASPV)、苹果褪绿叶斑病毒 (ACLSV)。 [22]
apple chlorotic fruit
Over the course of developing and applying a new real-time PCR assay for the detection of the newly described apple chlorotic fruit spot viroid (ACFSVd), slight modifications of the reverse transcription (RT) step were found to improve significantly the detection limit of the assay. [1] Based on our results and the demarcation criteria for viroids, the tentatively named “apple chlorotic fruit spot viroid” should be considered a putative new member of the genus Apscaviroid. [2]nan [1] 根据我们的研究结果和类病毒的划分标准，暂定名为“苹果褪绿果斑类病毒”应该被认为是拟拟病毒属的新成员。 [2]