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quantitative dot blot

Quantitative Dot Blot method (QDB) is proposed here to measure EGFR protein levels objectively and quantitatively.

Objective quantitation of EGFR protein levels using Quantitative Dot Blot (QDB) method for prognosis of gastric cancer patients

quantitative dot blot

Methods: The Ki67 protein levels were assessed using both IHC and Quantitative Dot Blot (QDB) methods respectively in 253 specimens.

Improving Prognosis of Surrogate Assay for Breast Cancer Patients by Absolute Quantitation of Ki67 Protein Levels using Quantitative Dot Blot (QDB) Method

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10.1101/2021.04.12.21255321

Quantitative Dot Blot method (QDB) is proposed here to measure EGFR protein levels objectively and quantitatively.

Objective quantitation of EGFR protein levels using Quantitative Dot Blot (QDB) method for prognosis of gastric cancer patients

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10.21203/RS.3.RS-437768/V1

Methods: The Ki67 protein levels were assessed using both IHC and Quantitative Dot Blot (QDB) methods respectively in 253 specimens.

Improving Prognosis of Surrogate Assay for Breast Cancer Patients by Absolute Quantitation of Ki67 Protein Levels using Quantitative Dot Blot (QDB) Method

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10.3389/fonc.2021.737781

Materials and Methods The Ki67 protein levels in a cohort of 253 specimens were assessed with IHC and quantitative dot blot (QDB) methods, respectively, and used to assign these specimens into Luminal A-like and Luminal B-like subtypes accordingly.

Improving Prognosis of Surrogate Assay for Breast Cancer Patients by Absolute Quantitation of Ki67 Protein Levels Using Quantitative Dot Blot (QDB) Method

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10.1093/ejcts/ezaa393

METHODS A semi-quantitative dot blot analysis was established to screen for preformed recipient antibodies using secondary anti-human antibodies.

Residual immune response towards decellularized homografts may be highly individual

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10.1101/584615

In this retrospective study, Quantitative Dot Blot (QDB) method was adopted to measure Her2 levels absolutely and quantitatively at protein level using 2X15 μm FFPE breast cancer tissue slices collected sequentially and non-selectively from local hospital.

Developing a routine lab test for absolute quantification of Her2 in Formalin Fixed Paraffin Embedded (FFPE) breast cancer tissues using Quantitative Dot Blot (QDB) method

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10.1101/584599

In this study, we explored the feasibility of Quantitative Dot Blot (QDB) method as a universal platform to quantitate tissue biomarkers as absolute and continuous variables in FFPE samples by measuring unprecedentedly the protein levels of Estrogen Receptor (ER), Progesterone Receptor (PR), Her2 and Ki67 simultaneously in 1048 FFPE samples.

3D association of ER, PR and Her2 at protein levels to aid sub-grouping of breast cancer patients in routine clinical practice

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10.1158/1538-7445.AM2019-3155

Methods: Quantitative Dot Blot (QDB) method was used to measure several biomarkers including ER, PR, Ki67, Her2, p53, PCNA in 1059 FFPE breast cancer tissues.

Abstract 3155: Multiple biomarker quantification as absolute and continuous variables in 1059 FFPE breast cancer specimens using QDB method

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10.1136/annrheumdis-2019-eular.6365

Anti-Ro/SSA, and anti-La/SSB antibodies were detected by semiquantitative dot blot method.

FRI0245 RHEUMATOID FACTOR IN IGA, IGG AND IGM IMMUNOGLOBULIN CLASES IN PRIMARY SJöGREN’S SYNDROME

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10.1186/s12014-019-9232-6

To perform the rapid identification of biomarkers, we adopted a high-throughput protein identification technique of Quantitative Dot Blot (QDB).

Identification of prothymosin alpha (PTMA) as a biomarker for esophageal squamous cell carcinoma (ESCC) by label-free quantitative proteomics and Quantitative Dot Blot (QDB)

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10.1016/j.ab.2019.04.003

To circumvent the limitations associated with sandwich ELISA for tissue biomarker quantitation, Quantitative Dot Blot method (QDB) was proposed using antibodies clinically validated for immunohistochemistry (IHC), as this method requires only one primary antibody in the analysis.

Quantitative Dot Blot (QDB) as a universal platform for absolute quantification of tissue biomarkers.

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Quantitative Dot - Quantitative Dot Blot method (QDB) is proposed here to measure EGFR protein levels objectively and quantitatively. [1] Methods: The Ki67 protein levels were assessed using both IHC and Quantitative Dot Blot (QDB) methods respectively in 253 specimens. [2] Materials and Methods The Ki67 protein levels in a cohort of 253 specimens were assessed with IHC and quantitative dot blot (QDB) methods, respectively, and used to assign these specimens into Luminal A-like and Luminal B-like subtypes accordingly. [3] METHODS A semi-quantitative dot blot analysis was established to screen for preformed recipient antibodies using secondary anti-human antibodies. [4] In this retrospective study, Quantitative Dot Blot (QDB) method was adopted to measure Her2 levels absolutely and quantitatively at protein level using 2X15 μm FFPE breast cancer tissue slices collected sequentially and non-selectively from local hospital. [5] In this study, we explored the feasibility of Quantitative Dot Blot (QDB) method as a universal platform to quantitate tissue biomarkers as absolute and continuous variables in FFPE samples by measuring unprecedentedly the protein levels of Estrogen Receptor (ER), Progesterone Receptor (PR), Her2 and Ki67 simultaneously in 1048 FFPE samples. [6] Methods: Quantitative Dot Blot (QDB) method was used to measure several biomarkers including ER, PR, Ki67, Her2, p53, PCNA in 1059 FFPE breast cancer tissues. [7] Anti-Ro/SSA, and anti-La/SSB antibodies were detected by semiquantitative dot blot method. [8] To perform the rapid identification of biomarkers, we adopted a high-throughput protein identification technique of Quantitative Dot Blot (QDB). [9] To circumvent the limitations associated with sandwich ELISA for tissue biomarker quantitation, Quantitative Dot Blot method (QDB) was proposed using antibodies clinically validated for immunohistochemistry (IHC), as this method requires only one primary antibody in the analysis. [10]

quantitative dot blot

Quantitative Dot Blot method (QDB) is proposed here to measure EGFR protein levels objectively and quantitatively. [1] Methods: The Ki67 protein levels were assessed using both IHC and Quantitative Dot Blot (QDB) methods respectively in 253 specimens. [2] Materials and Methods The Ki67 protein levels in a cohort of 253 specimens were assessed with IHC and quantitative dot blot (QDB) methods, respectively, and used to assign these specimens into Luminal A-like and Luminal B-like subtypes accordingly. [3] METHODS A semi-quantitative dot blot analysis was established to screen for preformed recipient antibodies using secondary anti-human antibodies. [4] In this retrospective study, Quantitative Dot Blot (QDB) method was adopted to measure Her2 levels absolutely and quantitatively at protein level using 2X15 μm FFPE breast cancer tissue slices collected sequentially and non-selectively from local hospital. [5] In this study, we explored the feasibility of Quantitative Dot Blot (QDB) method as a universal platform to quantitate tissue biomarkers as absolute and continuous variables in FFPE samples by measuring unprecedentedly the protein levels of Estrogen Receptor (ER), Progesterone Receptor (PR), Her2 and Ki67 simultaneously in 1048 FFPE samples. [6] Methods: Quantitative Dot Blot (QDB) method was used to measure several biomarkers including ER, PR, Ki67, Her2, p53, PCNA in 1059 FFPE breast cancer tissues. [7] Anti-Ro/SSA, and anti-La/SSB antibodies were detected by semiquantitative dot blot method. [8] To perform the rapid identification of biomarkers, we adopted a high-throughput protein identification technique of Quantitative Dot Blot (QDB). [9] To circumvent the limitations associated with sandwich ELISA for tissue biomarker quantitation, Quantitative Dot Blot method (QDB) was proposed using antibodies clinically validated for immunohistochemistry (IHC), as this method requires only one primary antibody in the analysis. [10]