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fluorescent polarization assay

Here we report a robust protein-based fluorescent polarization assay, that mimicking the formation of the six-helix bundle (6-HB) process during the membrane fusion, for the evaluation and screening of SARS-CoV-2 fusion Inhibitors.

A robust high-throughput fluorescent polarization assay for the evaluation and screening of SARS-CoV-2 fusion inhibitors

fluorescent polarization assay

Here we report a robust protein-based fluorescent polarization assay, that mimicking the formation of the six-helix bundle (6-HB) process during the membrane fusion, for the evaluation and screening of SARS-CoV-2 fusion Inhibitors.

A Robust High-throughput Fluorescent Polarization Assay for the Evaluation and Screening of SARS-CoV-2 Fusion Inhibitors

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10.1039/d0cb00187b

Here, we investigate STING binding by various fluorescein-labeled c-di-GMP, c-di-AMP and cGAMP, using fluorescent polarization (FP).

A STING-based fluorescent polarization assay for monitoring activities of cyclic dinucleotide metabolizing enzymes

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10.32008/geolinks2021/b1/v3/13

Cannabinoids may be detected by many and different analytical methods, including immunoassays (EMIT®, Elisa, fluorescent polarization, radioimmunotest), techniques of flat chromatography: classic thin layer chromatography (TLC), optimum performance laminar chromatography (OPLC) and multiple development automatization (AMD), gas chromatography-mass spectrometry (GC-MS), high-performance liquid chromatography-mass spectrometry (HPLC-MS).

METHODS FOR QUANTIFICATION OF THE MAIN CANNABINOIDS IN CBD OIL

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10.3390/ph12040168

In fact, advances in techniques such as fluorescence resonance energy transfer (FRET) and fluorescent polarization, as well as new applications in flow cytometry and different fluorescence-based microscopic techniques, are at the origin of the extensive research of new fluorescent ligands for these receptors.

Chemical Probes for the Adenosine Receptors

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10.1002/cbic.201900671

High‐throughput small‐molecule screening in drug discovery processes commonly rely on fluorescence‐based methods including fluorescent polarization and fluorescence/Förster resonance energy transfer.

Deploying Fluorescent Nucleoside Analogues for High‐Throughput Inhibitor Screening

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10.1186/s12964-019-0404-9

Western blotting, fluorescent polarization, computer simulation, and animal experiment were performed to investigate the molecular mechanism how CAPE reduces the stability of AR.

Caffeic acid phenethyl ester suppresses androgen receptor signaling and stability via inhibition of phosphorylation on Ser81 and Ser213

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10.1158/1538-7445.AM2019-4712

A fluorescent polarization (FP) competitive binding assay to the PBD of PLK1 was used to determine binding specificity.

Abstract 4712: Selective polo-like kinase 1 PBD inhibitor

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10.1158/1538-7445.AM2019-2754

The PPIs between Bcl-2 proteins and Hsp70 proteins were further validated by fluorescent polarization (FP), isothermal titration calorimetry (ITC) and Co-immunoprecipitation (Co-IP) experiments.

Abstract 2754: Affinity-based probe reveal Bim negatively regulates the chaperone functions of Hsp70, a non-Bcl-2 BH3 receptor

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What is/are Fluorescent Polarization?

Fluorescent Polarization - Here, we investigate STING binding by various fluorescein-labeled c-di-GMP, c-di-AMP and cGAMP, using fluorescent polarization (FP). [1] Cannabinoids may be detected by many and different analytical methods, including immunoassays (EMIT®, Elisa, fluorescent polarization, radioimmunotest), techniques of flat chromatography: classic thin layer chromatography (TLC), optimum performance laminar chromatography (OPLC) and multiple development automatization (AMD), gas chromatography-mass spectrometry (GC-MS), high-performance liquid chromatography-mass spectrometry (HPLC-MS). [2] In fact, advances in techniques such as fluorescence resonance energy transfer (FRET) and fluorescent polarization, as well as new applications in flow cytometry and different fluorescence-based microscopic techniques, are at the origin of the extensive research of new fluorescent ligands for these receptors. [3] High‐throughput small‐molecule screening in drug discovery processes commonly rely on fluorescence‐based methods including fluorescent polarization and fluorescence/Förster resonance energy transfer. [4] Western blotting, fluorescent polarization, computer simulation, and animal experiment were performed to investigate the molecular mechanism how CAPE reduces the stability of AR. [5] A fluorescent polarization (FP) competitive binding assay to the PBD of PLK1 was used to determine binding specificity. [6] The PPIs between Bcl-2 proteins and Hsp70 proteins were further validated by fluorescent polarization (FP), isothermal titration calorimetry (ITC) and Co-immunoprecipitation (Co-IP) experiments. [7]

fluorescent polarization assay

Here we report a robust protein-based fluorescent polarization assay, that mimicking the formation of the six-helix bundle (6-HB) process during the membrane fusion, for the evaluation and screening of SARS-CoV-2 fusion Inhibitors. [1] Here we report a robust protein-based fluorescent polarization assay, that mimicking the formation of the six-helix bundle (6-HB) process during the membrane fusion, for the evaluation and screening of SARS-CoV-2 fusion Inhibitors. [2] Serum from all the 3 boars, 16/22 sows and 9/25 gilts was obtained for serological examination by Buffered Plate Agglutination Test (BPAT), Rose Bengal Test (RBT) and Fluorescent Polarization Assay (FPA). [3] Rb1 competitively bound to ER-β, which was determined by the high sensitive fluorescent polarization assay. [4]