Introduction to Fluorescent Microscopy

We examined the effect of SW on lysosomal function using western blotting, transmission electron microscopy, fluorescent microscopy, and flow cytometry.

Dynamic light scattering (DLS), cryogenic transmission electron microscopy (cryo-TEM), optical and fluorescent microscopy are applied to study the stability and the insertion of the silicone oil into the softener.

Finally, the deposition of the new formulation on cotton fabrics is examined through scanning electron microscopy and a new protocol based on fluorescent microscopy.

Additionally, microplastics near a thin layer of water-air interphase were investigated using scanning electron microscopy, fluorescent microscopy, flow cytometry, and particle analyzers.

The transfection efficiency of PBMC cells was examined through evaluation of green fluorescent protein (GFP) expression using fluorescent microscopy.

The transfection method using cationic polymer was optimized based on N/P ratio, cell cytotoxicity, polyplex size, zeta potential and the green fluorescent protein (GFP) expression by fluorescent microscopy and flowcytometry.

The intracellular Ca2+ handling and contractile properties were also monitored using confocal fluorescent microscopy and atomic force microscopy.

Confocal fluorescent microscopy revealed that the Fggy-L variants appear to localize evenly throughout the cytoplasm, while the Fggy-S variants produce a more punctuate cytoplasmic localization pattern in proliferating muscle cells.

Here, to understand the spatial distribution and the potential sequestration of parasites in the whole-brain microvessels during a mouse model of ECM, we utilized the new tissue-clearing method CUBIC (Clear, Unobstructed, Brain/Body Imaging Cocktails and Computational analysis) with light sheet fluorescent microscopy (LSFM), and reconstructed images in three-dimensions (3D).

Therefore, light sheet fluorescent microscopy (LSFM) has become the method of choice for imaging cleared samples.

Using fluorescent microscopy and qPCR, we demonstrated that RZ2MS9-GFP successfully colonizes maize’s roots and leaves endophytically.

Using fluorescent microscopy, we tested whether acetazolamide directly inhibited store-operated calcium entry or calcium release from the sarcoplasmic reticulum, two well-documented sources of hypoxia-induced increases in [Ca2+]i in PASMCs.

In this study, we applied a viral vector-mediated expression of a reporter protein based on a UPR transcription factor, ATF4, and time-lapse fluorescent microscopy to elucidate how mouse primary neurons respond to pharmacological and genetic perturbations to neuronal proteostasis.

Using time-lapse fluorescent microscopy, we showed that fluorescently tagged Lsr2 forms large and dynamic nucleoprotein complexes, and that the N-terminal oligomerization domain of Lsr2 is indispensable for the formation of nucleoprotein complexes in vivo.

Fluorescent microscopy revealed that the functional Gmn2-EGFP fusion protein is mainly localized in the Golgi membrane.

A series of histochemical reactions and fluorescent microscopy revealed the various substances in the chemical composition of GTs.

In this study, we examined the performance of sparsity- and deep learning-based algorithms in reconstructing super-resolution images using simulated fluorescent microscopy images.

In addition, fluorescent microscopy images revealed that the ITO NPs emit strong fluorescence that could be used to reveal their location.

Current fluorescent microscopy techniques provide great tools to measure the recruitment kinetics of different repair proteins after DNA damage induction.

The amount of damage caused to their DNA was detected using the comet assay and fluorescent microscopy techniques.

The fluorescent microscopy results also indicated the increased apoptosis in magnetic field-assisted samples.

MTT assay on SK-BR-3 cell line (as breast cancer cells) and fluorescent microscopy results indicate that this modification decreases significantly drug toxicity and increases its selectivity.

Besides, biological assays such as flow cytometry and fluorescent microscopy confirmed the capacity of the secreted sf GFP-ANXV to detect PS exposure on apoptotic cells.

Caspase 3,7 activation, DNA damage, and fluorescent microscopy confirmed the apoptotic breast cancer response caused by targeted anti-CD71-CPSNPs encapsulated with gemcitabine monophosphate, the active metabolite of the chemotherapeutic gemcitabine used to treat cancers including breast and ovarian.

Analysis by immunofluorescent microscopy showed significantly impaired peroxisomal targeting signal 1- and peroxisomal targeting signal 2-mediated matrix protein import in both patient fibroblasts and PEX6 knockout cells.

Fluorescent microscopy showed that these nanocarriers were adsorbed on HeLa cells, unlike L929 cells.

Fluorescent microscopy observation and differential scanning calorimetry analysis disclosed that these ceramide analogues formed ceramide-rich phases in sphingomyelin bilayers, although their thermal stability was slightly inferior to that of normal ceramides.

Results obtained from autofluorescence spectroscopy of benign and malignant lower part gastrointestinal tract (GIT) lesions from freshly excised tissues during surgical removal of the lesions in 18 patients (22 lesions), were compared with the spectral measurements obtained during confocal fluorescent microscopy observations of unstained tissue slides using 405 nm excitation.

Briefly, through combining the photo-voltage approach with conventional fluorescent microscopy method, this work demonstrates new ideas on the time and membrane actions of polymer surfactants which should be taken into account for their biomedical applications.

The film formation was evaluated by fluorescent microscopy method.

We performed biochemical, molecular biology and fluorescent microscopy imaging experiments using gain- and loss-of-function mutants of tropomodulin 3 (Tmod3).

The warhead is easily synthesized from commercial starting materials and leads to potent probes which can be used for fluorescent in-gel protease detection and fluorescent microscopy imaging experiments.

Fluorescent microscopy indicates that expression of motB in vivo, at levels like that during T4 infection, yields a significantly compacted nucleoid containing MotB and H-NS.

The NMR measurements confirm binding to PEX14 in solution, while immunofluorescent microscopy indicates disruption of protein import into the glycosomes, indicating that the PEX14-PEX5 protein-protein interface was successfully disrupted.

The MTT assessment and fluorescent microscopy performed on fibroblasts and osteoblasts cells indicated a suitable cytocompatibility of the prepared alloys.

Fluorescent microscopy performed on in vitro and in vivo agglutination assays show that PCPP were entrapping ExPEC in a web-like network, thus demonstrating agglutination of ExPEC.

Fluorescent microscopy analysis showed apoptosis.

HrcQ complex formation was previously shown by fluorescent microscopy analysis and depends on the presence of the T3S system.