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Multiplex Fluorescent Immunohistochemistry

We characterized 285 early-stage endometrial carcinoma samples for T-cell infiltrates in a tissue microarray format using multiplex fluorescent immunohistochemistry.

Prognostic implications of tumor-infiltrating T cells in early-stage endometrial cancer

Multiplex Fluorescent Immunohistochemistry

In this study, we use material from two patient cohorts of early breast cancer and multiple methodologies (immunohistochemistry, RNA fluorescent in situ hybridization, immunofluorescence, bulk gene expression, and multiplex fluorescent immunohistochemistry) to demonstrate the significant discordance in PD-L1 expression among various methods and between different areas of the same tumor, which hints toward the presence of spatial, intratumoral and biological heterogeneity.

Discordance of PD-L1 Expression at the Protein and RNA Levels in Early Breast Cancer

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Multiplexed Fluorescent Immunohistochemistry

The aim of this proof-of-principle study is to describe immuno-genomic correlates within the tumor microenvironment using newly developed techniques for multiplexed fluorescent immunohistochemistry (mfIHC, Mezheyeuski et al.

Abstract PS18-27: Integrated immuno-genomic analyses in early breast cancer: Results from the Scandinavian breast group 2004-1 (SBG-2004-1) randomized phase II trial

Multiplexed Fluorescent Immunohistochemistry

Data from 40 PDAC patients that underwent surgical resection after NACRT (NACRT group) and 30 PDAC patients that underwent upfront surgery (US group) were analyzed to examine alterations in immune cell counts/distribution using a multiplexed fluorescent immunohistochemistry system.

The immunological impact of preoperative chemoradiotherapy on the tumor microenvironment of pancreatic cancer

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Performed Fluorescent Immunohistochemistry

In this study, we performed fluorescent immunohistochemistry staining to detect the infiltration of ‘anti-tumor’ cytotoxic T lymphocytes (CTLs) and ‘pro-tumor’ regulatory T cells (Tregs) in pre-S2 mutant-positive and -negative HCC patients.

Increased infiltration of regulatory T cells in hepatocellular carcinoma of patients with hepatitis B virus pre-S2 mutant

Performed Fluorescent Immunohistochemistry

To validate these observations, we performed fluorescent immunohistochemistry in the adult mouse hippocampus with either a CYFIP1 or CYFIP2 antibody combined with antibodies for various cell-type-specific markers.

Differential cell-type-expression of CYFIP1 and CYFIP2 in the adult mouse hippocampus

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Using Fluorescent Immunohistochemistry

Using fluorescent immunohistochemistry for c-Fos (neuronal activation), BrdU, and Neuronal Nuclei (NeuN), we investigated whether neurons born during reactive neurogenesis were incorporated into a newly learned MWM neuronal ensemble.

Functional Activation of Newborn Neurons Following Alcohol-Induced Reactive Neurogenesis

Using Fluorescent Immunohistochemistry

Methods Using fluorescent immunohistochemistry, Rab5 and Rab7 placental localization and comparative fluorescence intensity was explored in a cohort of placental tissues from pregnancies affected by maternal COVID-19 disease (“COVID”, n=15) in comparison with contemporary controls (“Control”, n=10).

Differential Expression of Rab5 and Rab7 Small GTPase Proteins in Placental Tissues From Pregnancies Affected by Maternal Coronavirus Disease 2019

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Underwent Fluorescent Immunohistochemistry

Biopsies were collected from 12 volunteers and underwent fluorescent immunohistochemistry (≥2 specimens per marker/structure).

Somatosensory innervation of healthy human oral tissues

Underwent Fluorescent Immunohistochemistry

Oral biopsies were collected from volunteers and underwent fluorescent immunohistochemistry followed by confocal imaging.

Localization of TRP channels in healthy oral mucosa from human donors

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Fluorescent Immunohistochemistry sentence examples within fluorescent immunohistochemistry method

fluorescent immunohistochemistry method

Endothelial cell markers CD31, CD146, and CD34 were observed using the fluorescent immunohistochemistry method.

Suppression of hypoxia and inflammatory pathways by Phyllanthus niruri extract inhibits angiogenesis in DMBA-induced breast cancer mice

fluorescent immunohistochemistry method

CD3, CD4, CD8, CD45RO, Foxp3, tumor necrosis factor receptor type II (TNFR2), programmed death ligand-1 (PD-L1), CD68, programmed death-1 (PD-1), cytokeratin (CK), and indoleamine 2,3-dioxygenase (IDO) were separated into two panels and stained using multiplex fluorescent immunohistochemistry methods.

Prognostic Value of the Neo-Immunoscore in Renal Cell Carcinoma

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fluorescent immunohistochemistry staining

In this study, we performed fluorescent immunohistochemistry staining to detect the infiltration of ‘anti-tumor’ cytotoxic T lymphocytes (CTLs) and ‘pro-tumor’ regulatory T cells (Tregs) in pre-S2 mutant-positive and -negative HCC patients.

Increased infiltration of regulatory T cells in hepatocellular carcinoma of patients with hepatitis B virus pre-S2 mutant

fluorescent immunohistochemistry staining

At 6 weeks after SCI, sagittal sections at the injured site and axial sections at L 4/5 were evaluated by fluorescent immunohistochemistry staining using S100B and glial fibrillary acidic protein (GFAP) antibodies.

Administration of ONO-2506 suppresses neuropathic pain after spinal cord injury by inhibition of astrocytic activation.

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10.1016/s0090-8258(21)01224-5

Molecular analyses were performed using fluorescent immunohistochemistry, TUNEL assays, and western blotting.

The novel NSAID-derivative, MCI-030, prevents ovarian cancer in the egg-laying hen by increasing tumor cell apoptosis and decreasing beta-catenin and MAPK oncogenic signaling

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10.13702/j.1000-0607.200615

The double-labeled neurons of Fos/vesicular glutamate transporter 2 (VGLUT2) in the VMPFC were detected by using fluorescent immunohistochemistry.

[Effect of electroacupuncture on morphine-conditioned place preference and activation of glutamatergic neurons in the ventromedial prefrontal cortex in rats].

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10.1016/j.ejphar.2021.173924

The mRNA and protein expressions of Kiss1r were measured by RT-PCR and Western blot analysis, while localizations of receptors were defined by fluorescent immunohistochemistry.

The ontogeny of kisspeptin receptor in the uterine contractions in rats: its possible role in the quiescence of non-pregnant and pregnant uteri.

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10.3390/cells10092410

TNFα expression was mapped by fluorescent immunohistochemistry and quantitative immunogold electron microscopy in myenteric ganglia of duodenum, ileum and colon.

Intestinal Region-Specific and Layer-Dependent Induction of TNFα in Rats with Streptozotocin-Induced Diabetes and after Insulin Replacement

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10.3390/biom11081163

By way of electroretinography, optical coherence tomography, funduscopy, fluorescein angiography, and fluorescent immunohistochemistry, we show degenerative and vascular phenotypes, microglial activation, photoreceptor phagocytosis, and upregulation of proinflammatory pathway proteins in the retinas of the human Q344X rhodopsin knock-in mouse.

Proinflammatory Pathways Are Activated in the Human Q344X Rhodopsin Knock-In Mouse Model of Retinitis Pigmentosa

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10.1016/j.biopha.2021.111971

In the present study, we found by fluorescent immunohistochemistry, that 7.

Astrocytes constitute the major TNF-α-producing cell population in the infarct cortex in dMCAO rats receiving intravenous MSC infusion.

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10.1186/s12974-020-02070-2

TNFα and hHSPB1 levels were measured from the supernates by ELISA, and intracellular hHSPB1 expression was analyzed using fluorescent immunohistochemistry.

Neuroinflammatory processes are augmented in mice overexpressing human heat-shock protein B1 following ethanol-induced brain injury

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10.1016/j.jchemneu.2020.101881

Fluorescent immunohistochemistry was performed on sections of rat brain covering the entire rostro-caudal extent of the SN and DRN with antibodies specific to the 5-HT3A receptor subunit, as well as others targeting the monoaminergic markers tyrosine hydroxylase (TH) and the 5-HT transporter (SERT).

Stereological investigation of 5-HT3 receptors in the substantia nigra and dorsal raphe nucleus in the rat

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10.1016/j.bbi.2020.10.012

Fluorescent immunohistochemistry was used to assess hippocampal parvalbumin cell density, intensity and co-expression with perineuronal nets.

Maternal immune activation targeted to a window of parvalbumin interneuron development improves spatial working memory: Implications for autism

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10.3389/froh.2021.740469

Tissue analysis was based on fluorescent immunohistochemistry (FIHC) and inflammatory mediators were analyzed using a Luminex-based 30-Plex panel.

Characterization of Oral Squamous Cell Carcinoma Associated Inflammation: A Pilot Study

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10.1371/journal.pone.0257847

Inflammatory responses were characterized by quantitative polymerase chain reaction (qPCR), histology, fluorescent immunohistochemistry, enzyme-linked inmmunosorbent assay (ELISA), fluorescent western blotting and blood chemistry analysis.

Direct administration of the non-competitive interleukin-1 receptor antagonist rytvela transiently reduced intrauterine inflammation in an extremely preterm sheep model of chorioamnionitis

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10.1089/can.2020.0004

On day 11, spinal cords were isolated and prepared for fluorescent immunohistochemistry.

Morphine Induces Upregulation of Neuronally Expressed CB2 Receptors in the Spinal Dorsal Horn of Rats.

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10.1016/J.BBMT.2018.12.096

Fluorescent immunohistochemistry (IHC) was used to further analyze zymogen granule protein 16B (ZG16B) in patient labial minor salivary glands (MSG).

Salivary Zymogen Granule Protein 16B Drops at Onset of Chronic Graft-Versus-Host Disease: A Possible Salivary Biomarker for Oral Chronic Graft-Versus-Host Disease

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10.1636/JoA-S-18-081

Protocols for rearing, fixation, fluorescent immunohistochemistry, colorimetric in situ hybridization, and imaging of embryos are detailed.

Embryogenesis in a Colorado population of Aphonopelma hentzi (Girard, 1852) (Araneae: Mygalomorphae: Theraphosidae): establishing a promising system for the study of mygalomorph development

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10.1152/japplphysiol.00624.2019

Various approaches interrogate fiber type at the single cell, but the two most commonly utilized are single muscle fiber sodium dodecyl sulfate polyacrylamide gel electrophoresis (smfSDS-PAGE) and fluorescent immunohistochemistry (IHC).

Cores of Reproducibility in Physiology (CORP): Fiber Typing Human Skeletal Muscle with Fluorescent Immunohistochemistry.

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10.1016/j.yhbeh.2018.11.003

To do this, using fluorescent immunohistochemistry, we examined the extent to which MOR and D1 receptors co‐localize in mPOA neurons and the degree to which photoperiod and the sex‐steroid hormone testosterone alter co‐localization.

Co-localization of mu-opioid and dopamine D1 receptors in the medial preoptic area and bed nucleus of the stria terminalis across seasonal states in male European starlings

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10.1007/s13562-019-00494-3

Most importantly, fluorescent immunohistochemistry of post-infected nodule sections show perceivable co-localization of these two proteins in the nodule symbiosomes.

Oxygen sequestration by Leghemoglobin is positively regulated via its interaction with another late nodulin, Nlj16 of Lotus japonicus

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10.1101/858894

Eight-week old male C57Bl/6 mice were orthotopically inoculated with 1×106 Gl261 cells and tumor morphology, local and systemic immune cell populations, and plasma cytokines/chemokines assessed at Day-0, 1, 3, 7, 14, and 21 post-inoculation by magnetic resonance imaging, chromogenic immunohistochemistry, multiplex immunofluorescent immunohistochemistry, flow cytometry and multiplex immunoassay respectively.

Temporal and spatial modulation of the immune response of the murine Gl261 glioma tumor microenvironment

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10.1101/742270

We establish cell type-specific adult cochlear supporting cell transcriptome profiles, and we validate these expression profiles through a combination of both fluorescent immunohistochemistry and in situ hybridization co-localization and qPCR of adult cochlear supporting cells.

Characterizing Adult cochlear supporting cell transcriptional diversity using single-cell RNA-Seq: Validation in the adult mouse and translational implications for the adult human cochlea

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10.1016/j.ebiom.2019.11.035

Methods hPG80 expression was monitored by fluorescent immunohistochemistry and mRNA expression in tumors from various origins.

The oncogenic and druggable hPG80 (Progastrin) is overexpressed in multiple cancers and detected in the blood of patients

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10.1177/1744806919838659

Fluorescent immunohistochemistry was employed to detect expressions of G6PD and TLR4 and co-location of G6PD with TLR4.

Downregulation of glucose-6-phosphate dehydrogenase contributes to diabetic neuropathic pain through upregulation of toll-like receptor 4 in rats

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10.1186/s13048-019-0586-1

Postmortem, H&E staining was performed on skeletal muscle sections and immunofluorescent immunohistochemistry was performed on skeletal muscle and tumor sections.

Withaferin A ameliorates ovarian cancer-induced cachexia and proinflammatory signaling

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10.3390/ijms20163887

The aim of the current study was to compare the expression of CHEM and its receptor (CHEM system) mRNAs (quantitative real-time PCR) and proteins (Western blotting and fluorescent immunohistochemistry) in the selected areas of the porcine hypothalamus responsible for gonadotropin-releasing hormone production and secretion: the mediobasal hypothalamus, preoptic area and stalk median eminence during the oestrous cycle and early pregnancy.

Expression of Chemerin and Its Receptors in the Porcine Hypothalamus and Plasma Chemerin Levels during the Oestrous Cycle and Early Pregnancy

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10.1016/bs.mcb.2018.09.001

reevesii have wide range of temperature tolerance between 15°C to 30°C and have high transparency, which can be a strong point in live-imaging and fluorescent immunohistochemistry.

Temnopleurus as an emerging echinoderm model.

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10.3892/ol.2019.11045

Immunohistochemistry for GFP, Vimentin, CD11b, CD31 and α-smooth muscle actin (SMA), and double-fluorescent immunohistochemistry for GFP-Vimentin, GFP-CD11b, GFP-CD31 and GFP-α-SMA was subsequently performed.

Differentiation and roles of bone marrow-derived cells on the tumor microenvironment of oral squamous cell carcinoma

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10.1177/1933719118773405

Spinal cords (T13-S1) from female C57BL/6 mice with endometriosis-like lesions (ENDO) were imaged via fluorescent immunohistochemistry for the expression of glial fibrillary acidic protein (GFAP; astrocytes) and CD11b (microglia) in the dorsal horn (n = 5).

Spinal Glial Adaptations Occur in a Minimally Invasive Mouse Model of Endometriosis: Potential Implications for Lesion Etiology and Persistent Pelvic Pain

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10.1089/ACU.2019.1334

Materials and Methods: Cholera toxin subunit B (CTB) was injected into LI 4 and LR 3 in different rats, and CTB neural labeling was examined using fluorescent immunohistochemistry and observed under fluorescent microscopy in the corresponding areas from the peripheral nervous system to the central nervous system, including the dorsal root ganglia (DRG), spinal cord, and brainstem.

Innervated Properties of Acupuncture Points LI 4 and LR 3 in the Rat: Neural Pathway Tracing with Cholera Toxin Subunit B.

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10.1016/j.bbr.2019.111984

Co-expression of PV and TrkB in the hippocampus was assessed by fluorescent immunohistochemistry and detailed stereology.

Sex-specific spatial memory deficits in mice with a conditional TrkB deletion on parvalbumin interneurons

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Fluorescent Immunohistochemistry
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What is/are Fluorescent Immunohistochemistry?

Fluorescent Immunohistochemistry - Molecular analyses were performed using fluorescent immunohistochemistry, TUNEL assays, and western blotting. [1] The double-labeled neurons of Fos/vesicular glutamate transporter 2 (VGLUT2) in the VMPFC were detected by using fluorescent immunohistochemistry. [2] The mRNA and protein expressions of Kiss1r were measured by RT-PCR and Western blot analysis, while localizations of receptors were defined by fluorescent immunohistochemistry. [3] TNFα expression was mapped by fluorescent immunohistochemistry and quantitative immunogold electron microscopy in myenteric ganglia of duodenum, ileum and colon. [4] By way of electroretinography, optical coherence tomography, funduscopy, fluorescein angiography, and fluorescent immunohistochemistry, we show degenerative and vascular phenotypes, microglial activation, photoreceptor phagocytosis, and upregulation of proinflammatory pathway proteins in the retinas of the human Q344X rhodopsin knock-in mouse. [5] In the present study, we found by fluorescent immunohistochemistry, that 7. [6] TNFα and hHSPB1 levels were measured from the supernates by ELISA, and intracellular hHSPB1 expression was analyzed using fluorescent immunohistochemistry. [7] Fluorescent immunohistochemistry was performed on sections of rat brain covering the entire rostro-caudal extent of the SN and DRN with antibodies specific to the 5-HT3A receptor subunit, as well as others targeting the monoaminergic markers tyrosine hydroxylase (TH) and the 5-HT transporter (SERT). [8] Fluorescent immunohistochemistry was used to assess hippocampal parvalbumin cell density, intensity and co-expression with perineuronal nets. [9] Tissue analysis was based on fluorescent immunohistochemistry (FIHC) and inflammatory mediators were analyzed using a Luminex-based 30-Plex panel. [10] Inflammatory responses were characterized by quantitative polymerase chain reaction (qPCR), histology, fluorescent immunohistochemistry, enzyme-linked inmmunosorbent assay (ELISA), fluorescent western blotting and blood chemistry analysis. [11] On day 11, spinal cords were isolated and prepared for fluorescent immunohistochemistry. [12] Fluorescent immunohistochemistry (IHC) was used to further analyze zymogen granule protein 16B (ZG16B) in patient labial minor salivary glands (MSG). [13] Protocols for rearing, fixation, fluorescent immunohistochemistry, colorimetric in situ hybridization, and imaging of embryos are detailed. [14] Various approaches interrogate fiber type at the single cell, but the two most commonly utilized are single muscle fiber sodium dodecyl sulfate polyacrylamide gel electrophoresis (smfSDS-PAGE) and fluorescent immunohistochemistry (IHC). [15] To do this, using fluorescent immunohistochemistry, we examined the extent to which MOR and D1 receptors co‐localize in mPOA neurons and the degree to which photoperiod and the sex‐steroid hormone testosterone alter co‐localization. [16] Most importantly, fluorescent immunohistochemistry of post-infected nodule sections show perceivable co-localization of these two proteins in the nodule symbiosomes. [17] Eight-week old male C57Bl/6 mice were orthotopically inoculated with 1×106 Gl261 cells and tumor morphology, local and systemic immune cell populations, and plasma cytokines/chemokines assessed at Day-0, 1, 3, 7, 14, and 21 post-inoculation by magnetic resonance imaging, chromogenic immunohistochemistry, multiplex immunofluorescent immunohistochemistry, flow cytometry and multiplex immunoassay respectively. [18] We establish cell type-specific adult cochlear supporting cell transcriptome profiles, and we validate these expression profiles through a combination of both fluorescent immunohistochemistry and in situ hybridization co-localization and qPCR of adult cochlear supporting cells. [19] Methods hPG80 expression was monitored by fluorescent immunohistochemistry and mRNA expression in tumors from various origins. [20] Fluorescent immunohistochemistry was employed to detect expressions of G6PD and TLR4 and co-location of G6PD with TLR4. [21] Postmortem, H&E staining was performed on skeletal muscle sections and immunofluorescent immunohistochemistry was performed on skeletal muscle and tumor sections. [22] The aim of the current study was to compare the expression of CHEM and its receptor (CHEM system) mRNAs (quantitative real-time PCR) and proteins (Western blotting and fluorescent immunohistochemistry) in the selected areas of the porcine hypothalamus responsible for gonadotropin-releasing hormone production and secretion: the mediobasal hypothalamus, preoptic area and stalk median eminence during the oestrous cycle and early pregnancy. [23] reevesii have wide range of temperature tolerance between 15°C to 30°C and have high transparency, which can be a strong point in live-imaging and fluorescent immunohistochemistry. [24] Immunohistochemistry for GFP, Vimentin, CD11b, CD31 and α-smooth muscle actin (SMA), and double-fluorescent immunohistochemistry for GFP-Vimentin, GFP-CD11b, GFP-CD31 and GFP-α-SMA was subsequently performed. [25] Spinal cords (T13-S1) from female C57BL/6 mice with endometriosis-like lesions (ENDO) were imaged via fluorescent immunohistochemistry for the expression of glial fibrillary acidic protein (GFAP; astrocytes) and CD11b (microglia) in the dorsal horn (n = 5). [26] Materials and Methods: Cholera toxin subunit B (CTB) was injected into LI 4 and LR 3 in different rats, and CTB neural labeling was examined using fluorescent immunohistochemistry and observed under fluorescent microscopy in the corresponding areas from the peripheral nervous system to the central nervous system, including the dorsal root ganglia (DRG), spinal cord, and brainstem. [27] Co-expression of PV and TrkB in the hippocampus was assessed by fluorescent immunohistochemistry and detailed stereology. [28]

Multiplex Fluorescent Immunohistochemistry

We characterized 285 early-stage endometrial carcinoma samples for T-cell infiltrates in a tissue microarray format using multiplex fluorescent immunohistochemistry. [1] In this study, we use material from two patient cohorts of early breast cancer and multiple methodologies (immunohistochemistry, RNA fluorescent in situ hybridization, immunofluorescence, bulk gene expression, and multiplex fluorescent immunohistochemistry) to demonstrate the significant discordance in PD-L1 expression among various methods and between different areas of the same tumor, which hints toward the presence of spatial, intratumoral and biological heterogeneity. [2] Biopsies were collected from 12 volunteers and underwent multiplex fluorescent immunohistochemistry (≥2 specimens per marker/structure). [3] Immune cell subsets (CD8+ T cell, macrophage, NK cell) in tumor and stromal were measured by multiplex fluorescent immunohistochemistry. [4] We obtained tumor tissues from 279 patients after esophagectomy and characterized the TME in intraepithelial and stromal regions using multiplex fluorescent immunohistochemistry (mfIHC). [5] CD3, CD4, CD8, CD45RO, Foxp3, tumor necrosis factor receptor type II (TNFR2), programmed death ligand-1 (PD-L1), CD68, programmed death-1 (PD-1), cytokeratin (CK), and indoleamine 2,3-dioxygenase (IDO) were separated into two panels and stained using multiplex fluorescent immunohistochemistry methods. [6] Background: Advances in multiplex fluorescent immunohistochemistry (mfIHC) techniques and digital pathology platforms allow the quantification of multiple proteins in the same tissue section and produce continuous data. [7] Multiplex fluorescent immunohistochemistry (mfIHC) bridges these technologies allowing for multi-epitope cellular phenotyping in formalin fixed paraffin embedded (FFPE) tissue while preserving the overall microenvironment architecture and spatial relationship of cells within intact undisrupted tissue. [8]

Multiplexed Fluorescent Immunohistochemistry

The aim of this proof-of-principle study is to describe immuno-genomic correlates within the tumor microenvironment using newly developed techniques for multiplexed fluorescent immunohistochemistry (mfIHC, Mezheyeuski et al. [1] Data from 40 PDAC patients that underwent surgical resection after NACRT (NACRT group) and 30 PDAC patients that underwent upfront surgery (US group) were analyzed to examine alterations in immune cell counts/distribution using a multiplexed fluorescent immunohistochemistry system. [2] We use multiplexed fluorescent immunohistochemistry (fIHC) to evaluate the co-localization of MYC, PLK1 and Ki67 to study their association with proliferation in DLBCL. [3] Multiplexed fluorescent immunohistochemistry for Ki67 (proliferation marker), cleaved PARP (apoptosis marker) and CAM 5. [4]

Performed Fluorescent Immunohistochemistry

In this study, we performed fluorescent immunohistochemistry staining to detect the infiltration of ‘anti-tumor’ cytotoxic T lymphocytes (CTLs) and ‘pro-tumor’ regulatory T cells (Tregs) in pre-S2 mutant-positive and -negative HCC patients. [1] To validate these observations, we performed fluorescent immunohistochemistry in the adult mouse hippocampus with either a CYFIP1 or CYFIP2 antibody combined with antibodies for various cell-type-specific markers. [2]

Using Fluorescent Immunohistochemistry

Using fluorescent immunohistochemistry for c-Fos (neuronal activation), BrdU, and Neuronal Nuclei (NeuN), we investigated whether neurons born during reactive neurogenesis were incorporated into a newly learned MWM neuronal ensemble. [1] Methods Using fluorescent immunohistochemistry, Rab5 and Rab7 placental localization and comparative fluorescence intensity was explored in a cohort of placental tissues from pregnancies affected by maternal COVID-19 disease (“COVID”, n=15) in comparison with contemporary controls (“Control”, n=10). [2]

Underwent Fluorescent Immunohistochemistry

Biopsies were collected from 12 volunteers and underwent fluorescent immunohistochemistry (≥2 specimens per marker/structure). [1] Oral biopsies were collected from volunteers and underwent fluorescent immunohistochemistry followed by confocal imaging. [2]

fluorescent immunohistochemistry method

Endothelial cell markers CD31, CD146, and CD34 were observed using the fluorescent immunohistochemistry method. [1] CD3, CD4, CD8, CD45RO, Foxp3, tumor necrosis factor receptor type II (TNFR2), programmed death ligand-1 (PD-L1), CD68, programmed death-1 (PD-1), cytokeratin (CK), and indoleamine 2,3-dioxygenase (IDO) were separated into two panels and stained using multiplex fluorescent immunohistochemistry methods. [2] The neurite outgrowth of DRG neurons was observed by fluorescent immunohistochemistry method. [3]

fluorescent immunohistochemistry staining

In this study, we performed fluorescent immunohistochemistry staining to detect the infiltration of ‘anti-tumor’ cytotoxic T lymphocytes (CTLs) and ‘pro-tumor’ regulatory T cells (Tregs) in pre-S2 mutant-positive and -negative HCC patients. [1] At 6 weeks after SCI, sagittal sections at the injured site and axial sections at L 4/5 were evaluated by fluorescent immunohistochemistry staining using S100B and glial fibrillary acidic protein (GFAP) antibodies. [2]