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Methods This study evaluated the sensitivity and specificity of four commercial SARS-CoV-2 lateral flow antibody tests using 213 serum specimens from 90 PCR-positive confirmed COVID-19 patients.

Evaluation of four commercial severe acute respiratory coronavirus 2 antibody tests

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The ability of four commercial SARS-CoV-2 serology platforms to detect previous infection or vaccination were evaluated using a cohort of 53 SARS-CoV-2 PCR-positive patients, 89 SARS-CoV-2-vaccinated healthcare workers (Pfizer or Moderna), and 127 SARS-CoV-2 negative patients.

SARS-CoV-2 Serology Status Detected by Commercialized Platforms Distinguishes Previous Infection and Vaccination Adaptive Immune Responses

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10.1016/j.bioelechem.2021.107982

A commercial SARS-CoV-2 S1 antigen was used to characterize the electrical output of the CNT-FET biosensor.

Carbon nanotube field-effect transistor (CNT-FET)-based biosensor for rapid detection of SARS-CoV-2 (COVID-19) surface spike protein S1

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10.1371/journal.pone.0246346

Commercial SARS-CoV2 IgG kit based on chemiluminescent assay was used for the selection of samples and optimization of the assay.

Development and performance evaluation of a rapid in-house ELISA for retrospective serosurveillance of SARS-CoV-2

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10.1016/j.jiph.2021.07.009

Background One major challenge for detecting the virus that causes COVID-19 is commercial SARS-CoV-2 testing kit or reagent availability.

Development and validation of an in-house, low-cost SARS-CoV-2 detection assay

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10.1016/j.cmi.2021.05.046

At present, the majority of commercial SARS-CoV-2 assays do not list saliva as an acceptable sample type for nucleic acid testing (NAT) and therefore validation studies assessing the use of this sample type are welcome.

Saliva testing for SARS-CoV-2 in children

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10.1038/s41598-021-99807-7

The samples were treated with lysis buffer and analyzed using both an in-house and a pre-commercial SARS-CoV-2 nucleocapsid antigen assay on Simoa.

Quantifying SARS-CoV-2 nucleocapsid antigen in oropharyngeal swabs using single molecule array technology

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10.3390/ijerph18189630

A total of 177 samples collected from COVID-19 suspected cases and 155 negative control sera collected during the pre-COVID-19 period were applied to evaluate the assay’s performance, with the plaque reduction neutralization test and the commercial SARS-CoV-2 spike protein IgG ELISA as gold standards.

Development and Evaluation of Quantitative Immunoglobulin G Enzyme-Linked Immunosorbent Assay for the Diagnosis of Coronavirus Disease 2019 Using Truncated Recombinant Nucleocapsid Protein as Assay Antigen

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10.21203/RS.3.RS-477479/V1

The samples were treated with lysis buffer and analyzed using both an in-house and a pre-commercial SARS-CoV-2 nucleocapsid antigen assay on Simoa.

Quantifying SARS-Cov-2 Nucleocapsid Antigen in Oropharyngeal Swabs Using Single Molecule Array Technology

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10.18231/J.IJPO.2021.004

SARS-CoV-2 IgG Ab titre and immune status ratio was estimated using a commercial SARS-CoV-2 Ig Ab detection ELISA kit.

Suspected SARS-Cov-2 reinfections in health care workers from Assam, India: Are they true reinfections?

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10.1101/2021.08.09.21261723

However, increased global demand has led to a shortage of commercial SARS-CoV-2 sequencing kits, and not every country has the technological capability or the funds for high-throughput sequencing platforms.

In-House, Rapid, and Low-Cost SARS-CoV-2 Spike Gene Sequencing Protocol to Identify Variants of Concern Using Sanger Sequencing

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10.1007/s10096-021-04169-7

Besides an overall discrepancy between the NT and the commercial assays regarding their sensitivity, this study revealed that commercial SARS-CoV-2 spike-based assays are better to predict the neutralization titer than nucleoprotein-based assays are.

Sensitivity of anti-SARS-CoV-2 serological assays in a high-prevalence setting

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What is/are Commercial Sars?

Commercial Sars - A commercial SARS-CoV-2 S1 antigen was used to characterize the electrical output of the CNT-FET biosensor. [1] Commercial SARS-CoV2 IgG kit based on chemiluminescent assay was used for the selection of samples and optimization of the assay. [2] Background One major challenge for detecting the virus that causes COVID-19 is commercial SARS-CoV-2 testing kit or reagent availability. [3] At present, the majority of commercial SARS-CoV-2 assays do not list saliva as an acceptable sample type for nucleic acid testing (NAT) and therefore validation studies assessing the use of this sample type are welcome. [4] The samples were treated with lysis buffer and analyzed using both an in-house and a pre-commercial SARS-CoV-2 nucleocapsid antigen assay on Simoa. [5] A total of 177 samples collected from COVID-19 suspected cases and 155 negative control sera collected during the pre-COVID-19 period were applied to evaluate the assay’s performance, with the plaque reduction neutralization test and the commercial SARS-CoV-2 spike protein IgG ELISA as gold standards. [6] The samples were treated with lysis buffer and analyzed using both an in-house and a pre-commercial SARS-CoV-2 nucleocapsid antigen assay on Simoa. [7] SARS-CoV-2 IgG Ab titre and immune status ratio was estimated using a commercial SARS-CoV-2 Ig Ab detection ELISA kit. [8] However, increased global demand has led to a shortage of commercial SARS-CoV-2 sequencing kits, and not every country has the technological capability or the funds for high-throughput sequencing platforms. [9] Besides an overall discrepancy between the NT and the commercial assays regarding their sensitivity, this study revealed that commercial SARS-CoV-2 spike-based assays are better to predict the neutralization titer than nucleoprotein-based assays are. [10]

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Methods This study evaluated the sensitivity and specificity of four commercial SARS-CoV-2 lateral flow antibody tests using 213 serum specimens from 90 PCR-positive confirmed COVID-19 patients. [1] The ability of four commercial SARS-CoV-2 serology platforms to detect previous infection or vaccination were evaluated using a cohort of 53 SARS-CoV-2 PCR-positive patients, 89 SARS-CoV-2-vaccinated healthcare workers (Pfizer or Moderna), and 127 SARS-CoV-2 negative patients. [2] A total of 1080 individual patient samples (158 positive serology samples from confirmed, predominantly mildly symptomatic COVID-19 patients and 922 serology negative including 496 collected pre-COVID) from four states in Australia were analysed on four commercial SARS-CoV-2 serological assays targeting antibodies to different antigens (Roche Elecsys and Abbott Architect: nucleocapsid; Diasorin Liaison and Euroimmun: spike). [3]