Nanopore Technology(나노포어 기술)란 무엇입니까?
Nanopore Technology 나노포어 기술 - Antibiotic susceptibility testing was performed, and the genomes were sequenced by Illumina and Nanopore technology. [1] The Nanopore technology offers a unique opportunity for the study of viral epitranscriptomics. [2] Nanopore technology can analyze single heteropolymer molecules such as DNA by measuring the ionic current flowing through a single nanometer hole made in an electrically insulating membrane. [3] While current proteomics techniques suffer from limitations in sensitivity and/or throughput, nanopore technology has the potential to enable de novo protein identification through single-molecule sequencing. [4] Membrane protein pores have demonstrated applications in nanopore technology. [5] We have provided the first assembled genome based on hybrid sequences from the highly diverse Eucalyptus subgenus Symphyomyrtus, and revealed the value of including long-reads from Nanopore technology for enhancing the contiguity of the assembled genome, as well as for improving its completeness. [6] Nanopore technology enables the detection and analysis of single protein molecules. [7] Isolates were characterised using DNA microarrays and one isolate from the United Arab Emirates was sequenced using Nanopore technology. [8] Here we describe a protocol for enrichment and full-length sequencing of circRNA isoforms using nanopore technology. [9] In recent years, nanopore technology has become increasingly important in the field of life science and biomedical research. [10] Recently, nanopore technology has enabled rapid sequencing with real-time analysis of pathogen DNA. [11] Diagnostics of OIAIs using shotgun metagenomics sequencing are possible within 24 h from biopsy using nanopore technology. [12] We sequenced ruminal samples from 416 Holstein dairy cows in 14 Spanish farms using nanopore technology, to uncover the presence of resistance genes and their potential effect on human, animal and environmental health. [13] These results demonstrated that the unzipping behaviors and DNA-protein interactions of hairpin and dumbbell DNA could be revealed using nanopore technology, and this could be further developed to create sensors for the secondary structures of nucleic acids. [14] Nanopore technology provides a new way to obtain kinetic and morphological aspects of Aβ aggregation at a single-molecule scale without labeling by detecting the electrochemical signal of the peptides when they pass through the hole. [15] Nanopore technology was used for sequencing CHIKV complete genomes. [16] Antibiotic susceptibility testing was performed and the genome was sequenced by Illumina and Nanopore technology. [17] Nanopore technology holds great promise for a wide range of applications such as biomedical sensing, chemical detection, desalination, and energy conversion. [18] This review provides an overview of different aspects and challenges of nanopore technology with a focus on chip-scale integration of solid-state nanopores for biosensing and bioanalytical applications. [19] We believe that our results will lay the foundation for rapid and selective sequencing using nanopore technology and will pave the way for future clinical applications using nanopore sequencing data. [20] In the iScience special issue ‘‘Single Molecule Technology – From Biotechnology to Biomedical Applications’’, guest edited by Amit Meller and Chirlmin Joo (Figure 1), we are highlighting a variety of research on nanopore technology, single molecule fluorescence, and a selection of other ultrasensitive detection methods. [21] Several rapid and cost-effective COVID-19 diagnostic techniques have also been devised based on nanobiosensors, lab-on-a-chip systems, or nanopore technology. [22] Unlike PacBio or the popular short-read sequencers before it, which, as examples of the second or so-called Next-Generation Sequencing platforms, need to synthesize when sequencing, nanopore technology directly sequences native DNA and RNA molecules. [23] Meanwhile, the whole genome of strain DW-1 was sequenced using nanopore technology. [24] Sequencing quality was comparable and lineage calling consistent for replicate samples processed with full volume Nextera DNA Flex (333 samples) or using nanopore technology (20 samples). [25] Whole genome sequencing was performed on 169 representative isolates with a combination of short and long reads using Illumina and Nanopore technology. [26] However, as nanopore technology is pushed to more and more epigenetic modifications, such complete training data will not be feasible to obtain. [27] This nanopore technology-based device has not been used previously for PAH diagnosis. [28] Nanopore technology was used for whole genome sequencing. [29] Here, we describe simple and rapid decoding of the DNA-computed output for a directed Hamiltonian path problem (HPP) using nanopore technology. [30] RESEARCH QUESTION Full-length 16S rRNA gene sequencing using nanopore technology is a fast alternative to conventional short-read 16S rRNA gene sequencing with low initial investment costs that has been used for various microbiome studies but has not yet been investigated as an alternative approach for endometrial microbiome analysis. [31] The patents describe and claim methods for using nanopore technology to sequence DNA and other nucleic acids. [32] Nanopore technology offers long, accurate sequencing of an DNA or RNA strand via enzymatic ratcheting of the strand through a nanopore in single nucleotide steps, producing stepwise modulations of the nanopore ion current. [33] Twenty-nine nasopharyngeal SARS-CoV-2 RNA positive samples were sequenced by nanopore technology (18 obtained at the initial period of the pandemic and 11 during the second wave) and analyzed them phylogenetically. [34] The accurate MiSeq sequencing data combined with the long reads obtained from the MinION platform of Nanopore technology allows for the resolution of the tested ambiguities. [35] Interest in nanopore technology has been growing due to nanopores' unique capabilities in small molecule sensing, measurement of protein folding, and low-cost DNA and RNA sequencing. [36] To help them better navigate this ever changing field, we discuss a range of software available to analyze sequences obtained using nanopore technology. [37] Even though several technical challenges remain to be addressed to exploit the potential of the nanopore technology fully, our work illustrates that direct RNA sequencing may significantly advance genomic studies of complex virus populations, including predictions on long-range interactions in individual full-length viral RNA haplotypes. [38] The nanopore technology is improving, and the sequencing community is likely to grow accordingly. [39] Conclusions Our analyses show that our method to amplify mtDNA and to sequence it using the nanopore technology is usable for mitochondrial DNA variant analysis. [40] With the development of nanopore technology, various kinds of nanopore sea water desalination methods have been reported, by controlling the pore size and functionalizing the nanopore, the nanopore can effectively reject salt ions. [41] In particular, the application of nanopore technology to real-sample analysis is limited in terms of discrim-inating multiple analytes in a mixture. [42] Nanopore technology was introduced for the study of the dynamic interactions between bovine serum albumin (BSA) and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) phospholipids based on a modified nanopore. [43] We used nanopore technology to generate chromosome-level assemblies for 3 different strains of Drechmeria coniospora, a nematophagous fungus used extensively in the study of innate immunity in Caenorhabditis elegans. [44] In a seminal work we provided a proof of concept of the novel ARTISAN-PCR amplification method, we adapted this technology for sequencing using Nanopore technology. [45] And in the future, the nanopore technology may be a rapid and label-free way for patients and doctors to evaluate the drugs’ efficiency. [46]항생제 감수성 테스트가 수행되었으며 게놈은 Illumina 및 Nanopore 기술로 시퀀싱되었습니다. [1] Nanopore 기술은 바이러스 epitranscriptomics 연구를 위한 독특한 기회를 제공합니다. [2] 나노포어(Nanopore) 기술은 전기 절연막에 뚫린 나노미터 구멍을 통해 흐르는 이온 전류를 측정해 DNA와 같은 단일 헤테로고분자 분자를 분석할 수 있다. [3] 현재의 proteomics 기술은 감도 및/또는 처리량에 제한이 있는 반면, nanopore 기술은 단일 분자 시퀀싱을 통해 새로운 단백질 식별을 가능하게 할 가능성이 있습니다. [4] 막 단백질 기공은 나노 기공 기술에서의 응용을 입증했습니다. [5] 우리는 매우 다양한 Eucalyptus 아속 Symphyomyrtus의 하이브리드 염기서열을 기반으로 한 최초의 조립된 게놈을 제공했으며, 조립된 게놈의 연속성을 높이고 완성도를 향상시키기 위해 Nanopore 기술의 긴 읽기를 포함하는 가치를 밝혔습니다. [6] Nanopore 기술은 단일 단백질 분자의 검출 및 분석을 가능하게 합니다. [7] 분리주는 DNA 마이크로어레이를 사용하여 특성화되었고 아랍에미리트에서 분리된 하나는 Nanopore 기술을 사용하여 시퀀싱되었습니다. [8] 여기에서 우리는 나노포어 기술을 사용하여 circRNA 이소폼의 농축 및 전체 길이 시퀀싱을 위한 프로토콜을 설명합니다. [9] 최근 몇 년 동안 나노포어 기술은 생명과학 및 생물의학 연구 분야에서 점점 더 중요해지고 있습니다. [10] 최근에는 나노포어 기술을 통해 병원체 DNA의 실시간 분석을 통해 신속한 시퀀싱이 가능해졌습니다. [11] 산탄총 메타게노믹스 시퀀싱을 사용하는 OIAI의 진단은 나노포어 기술을 사용하여 생검에서 24시간 이내에 가능합니다. [12] 우리는 나노포어 기술을 사용하여 14개의 스페인 농장에 있는 416마리의 홀스타인 젖소에서 반추위 샘플을 시퀀싱하여 저항성 유전자의 존재와 인간, 동물 및 환경 건강에 대한 잠재적 영향을 밝혀냈습니다. [13] 이러한 결과는 머리핀과 덤벨 DNA의 압축 풀기 동작과 DNA-단백질 상호작용이 나노포어 기술을 사용하여 밝혀질 수 있음을 보여주었고, 이것은 핵산의 2차 구조에 대한 센서를 만들기 위해 추가로 개발될 수 있습니다. [14] 나노포어 기술은 펩타이드가 구멍을 통과할 때 펩타이드의 전기화학적 신호를 감지함으로써 라벨링 없이 단일 분자 규모에서 Aβ 응집의 동역학 및 형태학적 측면을 얻을 수 있는 새로운 방법을 제공합니다. [15] Nanopore 기술은 CHIKV 완전한 게놈의 시퀀싱에 사용되었습니다. [16] 항생제 감수성 테스트가 수행되었고 게놈은 Illumina 및 Nanopore 기술로 시퀀싱되었습니다. [17] Nanopore 기술은 생물의학 감지, 화학물질 감지, 담수화 및 에너지 변환과 같은 광범위한 응용 분야에서 큰 가능성을 가지고 있습니다. [18] 이 리뷰는 바이오센싱 및 바이오분석 응용을 위한 고체 나노포어의 칩 규모 통합에 초점을 맞춘 나노포어 기술의 다양한 측면과 과제에 대한 개요를 제공합니다. [19] 우리는 우리의 결과가 나노포어 기술을 사용하여 신속하고 선택적인 시퀀싱을 위한 토대를 마련하고 나노포어 시퀀싱 데이터를 사용하는 미래 임상 응용을 위한 길을 닦을 것이라고 믿습니다. [20] Amit Meller와 Chirlmin Joo가 게스트 편집인 iScience 특별호 '단일 분자 기술 - 생명공학에서 생물의학 응용''(그림 1)에서 우리는 나노포어 기술, 단일 분자 형광 및 선택에 대한 다양한 연구를 강조하고 있습니다. 다른 초고감도 검출 방법. [21] 나노바이오센서, 랩온어칩 시스템 또는 나노포어 기술을 기반으로 몇 가지 신속하고 비용 효율적인 COVID-19 진단 기술이 고안되었습니다. [22] 두 번째 또는 소위 차세대 시퀀싱 플랫폼의 예로서 시퀀싱할 때 합성해야 하는 PacBio 또는 이전의 인기 있는 짧은 판독 시퀀서와 달리 나노포어 기술은 기본 DNA 및 RNA 분자를 직접 시퀀싱합니다. [23] 한편, 균주 DW-1의 전체 게놈은 나노포어 기술을 사용하여 시퀀싱되었다. [24] 전체 볼륨 Nextera DNA Flex(샘플 333개) 또는 나노포어 기술(샘플 20개)을 사용하여 처리된 복제 샘플에 대해 시퀀싱 품질이 비슷했으며 계보 호출이 일관되었습니다. [25] 전체 게놈 시퀀싱은 Illumina 및 Nanopore 기술을 사용하여 짧은 판독과 긴 판독의 조합으로 169개의 대표적인 분리주에서 수행되었습니다. [26] 그러나 나노포어 기술이 점점 더 많은 후성유전학적 변형으로 추진됨에 따라 이러한 완전한 훈련 데이터는 얻을 수 없을 것입니다. [27] 이 나노포어 기술 기반 장치는 이전에 PAH 진단에 사용되지 않았습니다. [28] Nanopore 기술은 전체 게놈 시퀀싱에 사용되었습니다. [29] 여기에서 우리는 나노포어 기술을 사용하여 방향성 해밀턴 경로 문제(HPP)에 대한 DNA 계산 출력의 간단하고 신속한 디코딩을 설명합니다. [30] 연구 질문 나노포어 기술을 사용한 전장 16S rRNA 유전자 시퀀싱은 초기 투자 비용이 낮은 기존의 short-read 16S rRNA 유전자 시퀀싱에 대한 빠른 대안으로, 다양한 미생물군집 연구에 사용되었지만 아직 자궁내막 미생물군유전체 분석을 위한 대체 접근 방식으로 조사되지 않았습니다. . [31] 특허는 DNA 및 기타 핵산의 서열을 결정하기 위해 나노포어 기술을 사용하는 방법을 설명하고 청구합니다. [32] 나노포어 기술은 단일 뉴클레오티드 단계에서 나노포어를 통한 가닥의 효소적 래칫팅을 통해 DNA 또는 RNA 가닥의 길고 정확한 시퀀싱을 제공하여 나노포어 이온 전류의 단계적 조절을 생성합니다. [33] 29개의 비인두 SARS-CoV-2 RNA 양성 샘플을 나노포어 기술(대유행 초기에 18개, 2차 파동 동안 11개)로 시퀀싱하고 계통발생학적으로 분석했습니다. [34] nan [35] nan [36] nan [37] nan [38] nan [39] nan [40] nan [41] nan [42] nan [43] nan [44] nan [45] nan [46]
long read sequencing
Long-read sequencing platforms recently developed by Oxford Nanopore Technology and Pacific Biosciences promise to overcome these limitations to deliver enhanced diagnosis of repeat expansion disorders in a rapid and cost-effective fashion. [1] However, with newly developed long-read sequencing technologies, such as Oxford Nanopore Technology Direct RNA sequencing (ONT DRS), combined with whole-genome bisulfite sequencing, we were able to precisely analyze the relationship between DNA methylation and pre-mRNA transcriptional initiation and processing using DNA methylation-related mutants. [2] Oxford Nanopore Technology (ONT) long-read sequencing has become a popular platform for microbial researchers; however, easy and automated construction of high-quality bacterial genomes remains challenging. [3] Results We sequenced, assembled, and annotated high-quality genomes for two Amoebophrya strains (A25 and A120), using a combination of Illumina paired-end short-read and Oxford Nanopore Technology (ONT) MinION long-read sequencing approaches. [4] In this study, we demonstrate the power of long-read sequencing for transcriptome annotation by coupling Oxford Nanopore Technology (ONT) with large-scale multiplexing of 93 samples, comprising 32 tissues collected from adult male and female Hereford cattle. [5] Background Oxford Nanopore Technology (ONT) long-read sequencing has become a popular platform for microbial researchers due to the accessibility and affordability of its devices. [6] To examine the global exon composition of circRNAs, we performed long-read sequencing of single molecules using nanopore technology for human and mouse brain-derived RNA. [7] The Oxford Nanopore Technology (ONT) GridION X5 was used for long-read sequencing with Illumina short-read data used to refine and generate high-quality, consensus assemblies. [8]Oxford Nanopore Technology와 Pacific Biosciences가 최근에 개발한 Long-read 시퀀싱 플랫폼은 이러한 한계를 극복하여 신속하고 비용 효율적인 방식으로 반복 확장 장애의 향상된 진단을 제공할 것을 약속합니다. [1] 그러나 Oxford Nanopore Technology Direct RNA sequencing(ONT DRS)과 같은 새로 개발된 long-read sequencing 기술과 whole-genome bisulfite sequencing을 결합하여 DNA 메틸화와 pre-mRNA 전사 개시 및 DNA 메틸화 관련 돌연변이를 사용한 처리. [2] nan [3] nan [4] nan [5] nan [6] nan [7] nan [8]
third generation long
With the advent of third generation long read sequencing technologies, including Oxford Nanopore Technology (ONT) and PacBio platforms, this problem is potentially surmountable. [1] Besides, because of its relevance, we also assess the best performing tool in third-generation long noisy read WGS data obtained with nanopore technology for a subset of the donors. [2] We also assessed the best performing tool in third-generation long noisy read WGS data obtained with nanopore technology for a subset of the donors. [3]Oxford Nanopore Technology(ONT) 및 PacBio 플랫폼을 포함한 3세대 긴 읽기 시퀀싱 기술의 출현으로 이 문제는 잠재적으로 극복할 수 있습니다. [1] 게다가, 관련성 때문에 우리는 기증자의 하위 집합에 대해 나노포어 기술로 얻은 3세대 긴 노이즈 읽기 WGS 데이터에서 최고의 성능 도구를 평가합니다. [2] nan [3]
Oxford Nanopore Technology
In this study, we isolated five NAD-independent strains from Korea and assembled their genomes using sequencing reads obtained from Illumina and Oxford Nanopore Technology platforms. [1] With the advent of third generation long read sequencing technologies, including Oxford Nanopore Technology (ONT) and PacBio platforms, this problem is potentially surmountable. [2] Results Updated Oxford Nanopore Technology software supported improved assemblies. [3] The viral sequencing was performed through oxford nanopore technology MinION platform. [4] Long-read sequencing platforms recently developed by Oxford Nanopore Technology and Pacific Biosciences promise to overcome these limitations to deliver enhanced diagnosis of repeat expansion disorders in a rapid and cost-effective fashion. [5] This protocol will enable producing longer Oxford Nanopore Technology reads for more plant samples and ushering forage grasses into modern genomics-assisted breeding programs. [6] These strains were further subjected to WGS using oxford nanopore technology MinION. [7] Here, we report on an optimized procedure based on long-reads produced on the ONT (Oxford Nanopore Technology) PromethION device to assemble the genome of the French bread wheat cultivar Renan. [8] To assemble a complete genome, we obtained short reads from Illumina sequencing and long reads from Oxford Nanopore Technology sequencing. [9] Here, RNA/cDNA-targeted sequencing (meta-transcriptomics using NGS (mtNGS)) is established to reduce the host nucleotide percentage in clinic samples and by combining with Oxford Nanopore Technology (ONT) platforms (meta-transcriptomics using third-generation sequencing, mtTGS) to improve the sequencing time. [10] Background One of the main advantages of the Oxford Nanopore Technology (ONT) is the possibility of real-time sequencing. [11] meleagridis were recruited using Oxford Nanopore Technology (ONT) and Illumina platforms, which were combined to generate megabase-sized contigs with high base-level accuracy. [12] However, with newly developed long-read sequencing technologies, such as Oxford Nanopore Technology Direct RNA sequencing (ONT DRS), combined with whole-genome bisulfite sequencing, we were able to precisely analyze the relationship between DNA methylation and pre-mRNA transcriptional initiation and processing using DNA methylation-related mutants. [13] Oxford Nanopore Technology (ONT) long-read sequencing has become a popular platform for microbial researchers; however, easy and automated construction of high-quality bacterial genomes remains challenging. [14] Hybrid assemblies of long (Oxford Nanopore Technology) and short (Illumina) sequence reads enabled the generation of 31 complete and 16 draft high quality genome sequences. [15] We explored the use of this locus as a molecular barcoding tool via the Oxford Nanopore Technology (ONT) sequencing platform MinION. [16] Summary MinoTour offers a LIMS system for Oxford Nanopore Technology (ONT) sequencers, with real-time metrics and analysis available permanently for review. [17] Assuming that the proteome is closely related to the transcriptome scaffold, quantitative PCR, Illumina HiSeq, and Oxford Nanopore Technology were applied to characterize the liver samples of three male donors in comparison with the HepG2 cell line. [18] Here we describe a simple method that achieves greater than tenfold enrichment of Arabidopsis thaliana 45S rRNA gene sequences among ultra-long Oxford Nanopore Technology sequencing reads. [19] The promising technology for reliable assessment of compound mutations in the KD of BCRABL1 are Oxford Nanopore technology or SMRT PacBio technology. [20] Here, we report a high-quality and almost complete Col-0 genome assembly with two gaps (Col-XJTU) using combination of Oxford Nanopore Technology ultra-long reads, PacBio high-fidelity long reads, and Hi-C data. [21] In this study, epitranscriptomic modifications caused by ammonium nutrition were monitored in maritime pine roots through direct RNA sequencing using Oxford Nanopore Technology. [22] METHODS Hybrid sequence of the study isolate was generated by using Illumina Hiseq and Oxford Nanopore Technology platforms. [23] graminearum isolate FG-12, which was isolated from the roots of maize seedlings exhibiting typical symptoms of blight growing in the Gansu province, China, using Oxford Nanopore Technology (ONT). [24] We aimed to develop a viral enrichment protocol for unbiased whole-genome sequencing of respiratory syncytial virus (RSV) from nasopharyngeal samples using the Oxford Nanopore Technology (ONT) MinION platform. [25] 37,266 contigs are preliminary assembled with Oxford Nanopore Technology (ONT) sequencing, totaling 804 Mb and GC content was 34. [26] In support of our hypothesis, we show Vulcan improves the alignments for Oxford Nanopore Technology (ONT) long-reads for both simulated and real datasets. [27] To contribute to the public health response and understand the diffusion of SARS-Cov-2 strains in Reunion Island, we established in-house genome sequencing capability in Reunion using Oxford nanopore technology (MinION) as an inexpensive option for genomic typing of SARS-CoV-2 lineages on the island, and cross-validated typing results between viral isolation methods and different sequencing technologies. [28] The entire (~ 1500 bp) 16S rRNA bacterial gene was fully amplified and sequenced using Oxford Nanopore technology. [29] Based on these results and a real-time sequencing experiment in which we demonstrated the feasibility of achieving sample-to-turnaround times of <30 hours with the Oxford Nanopore technology, we discuss the potential benefits of routine ultra-fast sequencing of all detected infections for contact tracing, infection cluster detection, and, ultimately, improved management of the SARS-CoV-2 pandemic. [30] One of the aforesaid technologies named ONT (Oxford Nanopore Technology) has attracted researchers in undertaking testings and experiments due to its affordability and accessibility. [31] Results Updated Oxford Nanopore Technology software supported improved assemblies. [32] Whole genome sequences employing Oxford nanopore technology were generated for 26 SARS-CoV-2 circulating in 10 different districts in Madhya Pradesh state of India. [33] WGS was performed using Oxford Nanopore Technology and protocols from the ARTIC network. [34] Oxford Nanopore Technology Direct RNA Sequencing (ONT DRS) also revealed that hda6 mutants with H3K18ac accumulation and CHG hypomethylation were shown to have transcriptionally active pericentromeric DNA. [35] Results We sequenced, assembled, and annotated high-quality genomes for two Amoebophrya strains (A25 and A120), using a combination of Illumina paired-end short-read and Oxford Nanopore Technology (ONT) MinION long-read sequencing approaches. [36] Here, we present a long read chromosome-level draft genome assembly and related plastid sequences using the Oxford Nanopore Technology PromethION platform and R10. [37] In addition, we demonstrated the use of the pocket-sized sequencer of Oxford Nanopore Technology to detect copy-number variants in HCC tissues that could be applied for onsite clinical detection/monitoring of HCC. [38] We report the complete genome sequence and annotation of “Candidatus Nardonella dryophthoridicola” strain NardRF, obtained by sequencing its host bacteriome, Rhynchophorus ferrugineus, using Oxford Nanopore technology. [39] A streaming assembly pipeline utilising real-time Oxford Nanopore Technology (ONT) sequencing data is important for saving sequencing resources and reducing time-to-result. [40] Reverse transcriptase polymerase chain reaction (RT-PCR) was used to amplify the whole norovirus genome, followed by next-generation sequencing using Oxford Nanopore technology. [41] We employed Oxford Nanopore technology to sequence genomic DNAs from a pool of adult worms of the carcinogenic parasite, Schistosoma haematobium, and used an informatic workflow to define the complete mt non-coding region(s). [42] Here, we describe our methodological approach to using Oxford Nanopore Technology (ONT) sequencing data to quantify genetic relatedness and to look for microevolutionary events in the core and accessory genomes to assess the within-outbreak variation of four genetically and epidemiologically linked isolates. [43] Using Oxford Nanopore Technology (ONT), Nottingham-Indonesia Collaboration for Clinical Research and Training (NICCRAT) initiative has facilitated collaboration between the University of Nottingham and a team in Research Centre for Biotechnology, Indonesian Institute of Sciences (Lembaga Ilmu Pengetahuan Indonesia/LIPI) to carry out a small number of SARS-CoV-2 WGS in Indonesia. [44] However, Oxford Nanopore Technology (ONT) provides significant improvements in accessibility, turnaround time and portability. [45] Recent advances in next-generation sequencing technologies like Oxford Nanopore Technology (ONT) have helped in the generation of longer reads of DNA in a shorter duration with lower cost. [46] In this study, we demonstrate the power of long-read sequencing for transcriptome annotation by coupling Oxford Nanopore Technology (ONT) with large-scale multiplexing of 93 samples, comprising 32 tissues collected from adult male and female Hereford cattle. [47] SARS-CoV-2 viral sequencing was performed using Oxford Nanopore Technology. [48] The sequences generated by PacBio or Oxford Nanopore Technology (ONT) be assembled de novo before further analyses. [49] A long read amplicon sequencing approach based on the Oxford Nanopore technology, targeting the spike protein, was applied to detect SARS-CoV-2 variants in sewage samples collected in central Italy on April 2021. [50]이 연구에서 우리는 한국에서 5개의 NAD 독립 균주를 분리하고 Illumina 및 Oxford Nanopore Technology 플랫폼에서 얻은 시퀀싱 읽기를 사용하여 게놈을 조립했습니다. [1] Oxford Nanopore Technology(ONT) 및 PacBio 플랫폼을 포함한 3세대 긴 읽기 시퀀싱 기술의 출현으로 이 문제는 잠재적으로 극복할 수 있습니다. [2] 결과 업데이트된 Oxford Nanopore Technology 소프트웨어는 향상된 어셈블리를 지원했습니다. [3] 바이러스 시퀀싱은 옥스포드 나노포어 기술인 MinION 플랫폼을 통해 수행되었습니다. [4] Oxford Nanopore Technology와 Pacific Biosciences가 최근에 개발한 Long-read 시퀀싱 플랫폼은 이러한 한계를 극복하여 신속하고 비용 효율적인 방식으로 반복 확장 장애의 향상된 진단을 제공할 것을 약속합니다. [5] 이 프로토콜은 더 많은 식물 샘플에 대해 더 긴 Oxford Nanopore Technology 판독값을 생성하고 마초 풀을 현대 유전체학 지원 육종 프로그램으로 안내합니다. [6] 이러한 균주는 옥스포드 나노포어 기술인 MinION을 사용하여 WGS에 추가로 적용되었습니다. [7] nan [8] 완전한 게놈을 조립하기 위해 Illumina 시퀀싱에서 짧은 읽기를 얻었고 Oxford Nanopore Technology 시퀀싱에서 긴 읽기를 얻었습니다. [9] nan [10] nan [11] nan [12] 그러나 Oxford Nanopore Technology Direct RNA sequencing(ONT DRS)과 같은 새로 개발된 long-read sequencing 기술과 whole-genome bisulfite sequencing을 결합하여 DNA 메틸화와 pre-mRNA 전사 개시 및 DNA 메틸화 관련 돌연변이를 사용한 처리. [13] nan [14] nan [15] nan [16] nan [17] nan [18] 여기에서 우리는 매우 긴 Oxford Nanopore Technology 시퀀싱 읽기 중에서 Arabidopsis thaliana 45S rRNA 유전자 서열의 10배 이상의 농축을 달성하는 간단한 방법을 설명합니다. [19] nan [20] 여기에서 우리는 Oxford Nanopore Technology의 초장기 판독, PacBio 고충실도 긴 판독 및 Hi-C 데이터의 조합을 사용하여 2개의 간격(Col-XJTU)이 있는 고품질의 거의 완전한 Col-0 게놈 어셈블리를 보고합니다. [21] nan [22] 행동 양식 연구 분리주의 하이브리드 시퀀스는 Illumina Hiseq 및 Oxford Nanopore Technology 플랫폼을 사용하여 생성되었습니다. [23] nan [24] nan [25] nan [26] nan [27] nan [28] nan [29] nan [30] nan [31] 결과 업데이트된 Oxford Nanopore Technology 소프트웨어는 향상된 어셈블리를 지원했습니다. [32] nan [33] nan [34] Oxford Nanopore Technology Direct RNA Sequencing(ONT DRS)은 또한 H3K18ac 축적 및 CHG 저메틸화를 갖는 hda6 돌연변이체가 전사적으로 활성인 중심체 중심체 DNA를 갖는 것으로 나타났음을 밝혔습니다. [35] nan [36] nan [37] nan [38] nan [39] nan [40] nan [41] nan [42] nan [43] nan [44] nan [45] nan [46] nan [47] nan [48] nan [49] nan [50]
State Nanopore Technology
Solid-state nanopore technology delivers single-molecule resolution information, and the quality of the deliverables hinges on the capability of the analysis platform to extract maximum possible events and fit them appropriately. [1] Solid-state nanopore technology presents an emerging single-molecule-based analytical tool for the separation and analysis of nanoparticles. [2]고체 상태 나노포어 기술은 단일 분자 분해능 정보를 제공하며, 결과물의 품질은 가능한 최대 이벤트를 추출하고 적절하게 맞추는 분석 플랫폼의 기능에 달려 있습니다. [1] nan [2]
nanopore technology sequencing
To assemble a complete genome, we obtained short reads from Illumina sequencing and long reads from Oxford Nanopore Technology sequencing. [1] Here we describe a simple method that achieves greater than tenfold enrichment of Arabidopsis thaliana 45S rRNA gene sequences among ultra-long Oxford Nanopore Technology sequencing reads. [2] In order to assemble a complete genome we obtained short-reads from Illumina sequencing as well as long-reads from Oxford Nanopore Technology sequencing. [3]완전한 게놈을 조립하기 위해 Illumina 시퀀싱에서 짧은 읽기를 얻었고 Oxford Nanopore Technology 시퀀싱에서 긴 읽기를 얻었습니다. [1] 여기에서 우리는 매우 긴 Oxford Nanopore Technology 시퀀싱 읽기 중에서 Arabidopsis thaliana 45S rRNA 유전자 서열의 10배 이상의 농축을 달성하는 간단한 방법을 설명합니다. [2] nan [3]
nanopore technology read
This protocol will enable producing longer Oxford Nanopore Technology reads for more plant samples and ushering forage grasses into modern genomics-assisted breeding programs. [1] The combination of Illumina paired-end, Illumina mate-pair and Oxford Nanopore Technology reads greatly improved the assembly of the H. [2]이 프로토콜은 더 많은 식물 샘플에 대해 더 긴 Oxford Nanopore Technology 판독값을 생성하고 마초 풀을 현대 유전체학 지원 육종 프로그램으로 안내합니다. [1] nan [2]
nanopore technology platform
In this study, we isolated five NAD-independent strains from Korea and assembled their genomes using sequencing reads obtained from Illumina and Oxford Nanopore Technology platforms. [1] METHODS Hybrid sequence of the study isolate was generated by using Illumina Hiseq and Oxford Nanopore Technology platforms. [2]이 연구에서 우리는 한국에서 5개의 NAD 독립 균주를 분리하고 Illumina 및 Oxford Nanopore Technology 플랫폼에서 얻은 시퀀싱 읽기를 사용하여 게놈을 조립했습니다. [1] 행동 양식 연구 분리주의 하이브리드 시퀀스는 Illumina Hiseq 및 Oxford Nanopore Technology 플랫폼을 사용하여 생성되었습니다. [2]
nanopore technology offer
The Nanopore technology offers a unique opportunity for the study of viral epitranscriptomics. [1] Nanopore technology offers long, accurate sequencing of an DNA or RNA strand via enzymatic ratcheting of the strand through a nanopore in single nucleotide steps, producing stepwise modulations of the nanopore ion current. [2]Nanopore 기술은 바이러스 epitranscriptomics 연구를 위한 독특한 기회를 제공합니다. [1] 나노포어 기술은 단일 뉴클레오티드 단계에서 나노포어를 통한 가닥의 효소적 래칫팅을 통해 DNA 또는 RNA 가닥의 길고 정확한 시퀀싱을 제공하여 나노포어 이온 전류의 단계적 조절을 생성합니다. [2]
nanopore technology software
Results Updated Oxford Nanopore Technology software supported improved assemblies. [1] Results Updated Oxford Nanopore Technology software supported improved assemblies. [2]결과 업데이트된 Oxford Nanopore Technology 소프트웨어는 향상된 어셈블리를 지원했습니다. [1] 결과 업데이트된 Oxford Nanopore Technology 소프트웨어는 향상된 어셈블리를 지원했습니다. [2]
nanopore technology minion
The viral sequencing was performed through oxford nanopore technology MinION platform. [1] These strains were further subjected to WGS using oxford nanopore technology MinION. [2]바이러스 시퀀싱은 옥스포드 나노포어 기술인 MinION 플랫폼을 통해 수행되었습니다. [1] 이러한 균주는 옥스포드 나노포어 기술인 MinION을 사용하여 WGS에 추가로 적용되었습니다. [2]
nanopore technology direct
However, with newly developed long-read sequencing technologies, such as Oxford Nanopore Technology Direct RNA sequencing (ONT DRS), combined with whole-genome bisulfite sequencing, we were able to precisely analyze the relationship between DNA methylation and pre-mRNA transcriptional initiation and processing using DNA methylation-related mutants. [1] Oxford Nanopore Technology Direct RNA Sequencing (ONT DRS) also revealed that hda6 mutants with H3K18ac accumulation and CHG hypomethylation were shown to have transcriptionally active pericentromeric DNA. [2]그러나 Oxford Nanopore Technology Direct RNA sequencing(ONT DRS)과 같은 새로 개발된 long-read sequencing 기술과 whole-genome bisulfite sequencing을 결합하여 DNA 메틸화와 pre-mRNA 전사 개시 및 DNA 메틸화 관련 돌연변이를 사용한 처리. [1] Oxford Nanopore Technology Direct RNA Sequencing(ONT DRS)은 또한 H3K18ac 축적 및 CHG 저메틸화를 갖는 hda6 돌연변이체가 전사적으로 활성인 중심체 중심체 DNA를 갖는 것으로 나타났음을 밝혔습니다. [2]
nanopore technology ultra
Here, we report a high-quality and almost complete Col-0 genome assembly with two gaps (Col-XJTU) using combination of Oxford Nanopore Technology ultra-long reads, PacBio high-fidelity long reads, and Hi-C data. [1] Here, we report a high-quality and almost complete Col-0 genome assembly with two gaps (Col-XJTU) using combination of Oxford Nanopore Technology ultra-long reads, PacBio high-fidelity long reads, and Hi-C data. [2]여기에서 우리는 Oxford Nanopore Technology의 초장기 판독, PacBio 고충실도 긴 판독 및 Hi-C 데이터의 조합을 사용하여 2개의 간격(Col-XJTU)이 있는 고품질의 거의 완전한 Col-0 게놈 어셈블리를 보고합니다. [1] 여기에서 우리는 Oxford Nanopore Technology의 초장기 판독, PacBio 고충실도 긴 판독 및 Hi-C 데이터의 조합을 사용하여 2개의 간격(Col-XJTU)이 있는 고품질의 거의 완전한 Col-0 게놈 어셈블리를 보고합니다. [2]