Illumination Microscopy(조명 현미경)란 무엇입니까?
Illumination Microscopy 조명 현미경 - By combining oblique back-illumination microscopy and a z-splitter prism, we perform phase imaging that is both epi-mode and multifocus, enabling high-speed 3D phase imaging in thick, scattering tissues with a single camera. [1] Nano-Illumination Microscopy (NIM) is a technique that provides compact microscopes but at the present time only setups with limited Field-of-View (FOV) have been presented. [2] By combining oblique back-illumination microscopy and a z-splitter prism, we perform phase imaging that is both epi-mode and multifocus, enabling high-speed 3D phase imaging in thick, scattering tissues with a single camera. [3] This study provides a technique for distinguishing osteochondrosis and osteochondritis and further documents of the value of epi-illumination microscopy in expanding our understanding of bone and joint disease. [4] In this work we present a new microscope based on Nano-illumination microscopy (NIM), i. [5]비스듬한 후면 조명 현미경과 z-분할 프리즘을 결합하여 에피 모드와 다초점 위상 이미징을 수행하여 단일 카메라로 두꺼운 산란 조직에서 고속 3D 위상 이미징을 가능하게 합니다. [1] NIM(Nano-Illumination Microscopy)은 소형 현미경을 제공하는 기술이지만 현재로서는 제한된 시야(FOV)가 있는 설정만 제시되었습니다. [2] 비스듬한 이면조사 현미경과 z-분할 프리즘을 결합하여 에피 모드와 다초점 위상 이미징을 수행하여 단일 카메라로 두꺼운 산란 조직에서 고속 3D 위상 이미징을 가능하게 합니다. [3] 이 연구는 골연골증과 골연골염을 구별하는 기술과 뼈와 관절 질환에 대한 이해를 확장하는 데 에피조명 현미경의 가치에 대한 추가 문서를 제공합니다. [4] 이 작업에서 우리는 나노 조명 현미경(NIM)에 기반한 새로운 현미경을 제시합니다. [5]
super resolution structured 초해상도 구조화
Here, using super resolution structured illumination microscopy, we find that neither CHMP4B nor CHMP2B are increased in ALS neuronal nuclei. [1] In this study, we developed a technique that allows recapitulation of bristle actin module organization using the Drosophila ovary by a combination of confocal microscopy, super-resolution structured illumination microscopy, and correlative light and electron microscope analysis. [2] Super-resolution structured illumination microscopy (SIM) routinely performs image reconstruction in the frequency domain using an approach termed frequency-domain reconstruction (FDR). [3] Here, we use optical sectioning super-resolution structured illumination microscopy (OS-SR-SIM) with a low magnification objective. [4] Super-resolution structured illumination microscopy (SR-SIM) provides an up to two-fold enhanced spatial resolution of fluorescently labeled samples. [5] Here we describe a detailed protocol for both super-resolution structured illumination microscopy (SR-SIM) as well as direct stochastic optical reconstruction microscopy (dSTORM) for the visualization of key proteins associated with the autophagy molecular machinery and cargo. [6] Here we determined by super-resolution structured illumination microscopy that FAP70 is located exclusively in the CA, and show by cryo-electron microscopy that its N-terminus is located at the base of the CA's C2a projection. [7] We collected human inner ear material for nanoscale visualization combining transmission electron microscopy (TEM), super-resolution structured illumination microscopy (SR-SIM), and RNA-scope analysis for the first time. [8] Super-resolution structured illumination microscopy (SIM) has become a widely used method for biological imaging. [9] To investigate whether AGK-BRAF and RET/PTC3 are associated with genomic instability and chromatin modifications, we performed quantitative fluorescence in situ hybridization (Q-FISH) of telomere repeats followed by 3D imaging analysis and 3D super-resolution Structured Illumination Microscopy (3D-SIM) to analyze the DNA structure from the foci. [10] With super-resolution structured illumination microscopy (SIM) revealing axonal structures, here we imaged the lattice structure of completely assembled AIS in APP/PS1 neurons. [11] Super-resolution structured illumination microscopy (SIM) has become a widely used method for biological imaging. [12] Of the commonly used techniques, super-resolution structured illumination microscopy (SIM) affords the unparalleled opportunity to study the localization and expression of individual Nups using conventional antibody-based labeling strategies. [13] Super-resolution structured illumination microscopy (SR-SIM) provides an elegant way of overcoming the diffraction limit in conventional widefield microscope by superimposing a grid pattern generated through interference of diffraction orders on the specimen while capturing images. [14] We detected maize miR2275 by super-resolution structured illumination microscopy and direct stochastic optical reconstruction microscopy. [15] Results from the Super-Resolution Structured Illumination Microscopy (SR-SIM) analysis showed that Vpx puncta alter HeLa cell nuclear envelope assembly. [16] The impact of the different reconstruction parameters in super-resolution structured illumination microscopy (SIM) on image artifacts is carefully analyzed. [17] Super resolution structured illumination microscopy (SIM) was used to identify an association between NP and components of the R2TP complex, which includes RUVBL1, RUVBL2, RPAP3, and PIH1D1, suggesting a potential role for the R2TP complex in capsid formation. [18] We propose a method combining super-resolution structured illumination microscopy (SIM) and the wide-field narrow-bandwidth filtering technique of the tunable filter, using a digital micro mirror device (DMD) to generate structured patterns, and a pair of filters whose cutoff frequency can change as the angle of incidence changes, which allows us to obtain super-resolution images of samples at specific Raman shift peaks. [19]여기에서 초해상도 구조 조명 현미경을 사용하여 ALS 신경 핵에서 CHMP4B와 CHMP2B가 모두 증가하지 않는다는 것을 발견했습니다. [1] 이 연구에서 우리는 공초점 현미경, 초해상도 구조 조명 현미경, 상관광 및 전자 현미경 분석을 조합하여 초파리 난소를 사용하여 강모 액틴 모듈 조직을 요약할 수 있는 기술을 개발했습니다. [2] nan [3] nan [4] nan [5] nan [6] nan [7] nan [8] nan [9] nan [10] nan [11] nan [12] nan [13] nan [14] nan [15] nan [16] nan [17] nan [18] nan [19]
super resolution microscopy 초고해상도 현미경
Structured illumination microscopy (SIM) imaging on live Bacillus cereus confirmed the suitability of the probe for super-resolution microscopy. [1] In both PLP1 patients' and healthy fibroblasts , we measured mitochondrial respiration with a Seahorse XF Extracellular Analyzer and examined the interactions between the ER and mitochondria with super-resolution microscopy (spinning-disc pinhole-based structured illumination microscopy, SD-SIM). [2] We also used super resolution microscopy (Structured Illumination microscopy or SIM) in conjunction with immunohistochemistry to assess changes in the number and organization of "osteocyte mechanosomes" - complexes of Panx1 channels, P2X7 receptors and CaV3 voltage-gated Ca2+ channels clustered around αvβ3 integrin foci on osteocyte processes. [3] Various advanced super-resolution microscopy techniques, such as stimulated emission depletion (STED), structured illumination microscopy (SIM), and single-molecule localization microscopy (SMLM), bypass the diffraction limit and provide a sub-diffraction-limit resolving power, ranging from 10 to 100 nm. [4] Structured illumination microscopy (SIM) imaging on live Bacillus cereus confirmed the suitability of the probe for super-resolution microscopy. [5] Linear structured illumination microscopy (SIM) is a super-resolution microscopy technique that does not impose photophysics requirements on fluorescent samples. [6] While structured illumination microscopy (SIM) is a widely used super-resolution microscopy technique for densely labelled samples, we apply a modified version of the matrix pencil method to single molecule imaging. [7] In both PLP1 patients’ and healthy fibroblasts, we measured mitochondrial respiration with a Seahorse XF Extracellular Analyzer and examined the interactions between the ER and mitochondria with super-resolution microscopy (spinning-disc pinhole-based structured illumination microscopy, SD-SIM). [8] Structured illumination microscopy (SIM) is an essential super-resolution microscopy technique that enhances resolution. [9] Meanwhile, super-resolution microscopy, such as structured illumination microscopy (SIM), is being applied more and more to overcome this restriction. [10] In super-resolution microscopy applications that use light modulation, most notably structured illumination microscopy (SIM), the coherent nature of the excitation light becomes a requirement to achieve optimal interference pattern contrast. [11] Structured illumination microscopy (SIM) uses a relatively low illumination light power compared with other super-resolution microscopies and has great potential to meet the demands of live-cell imaging. [12] In the context of various approaches to super-resolution microscopy, structured illumination microscopy (SIM) offers several advantages: it needs rather low light doses (with a low risk of phototoxicity or photobleaching), is comparably fast and flexible concerning the use of microscopes, objective lenses and cameras, and has potential for 3D imaging. [13]살아있는 Bacillus cereus에 대한 SIM(Structured Illumination Microscopy) 이미징은 초해상도 현미경에 대한 프로브의 적합성을 확인했습니다. [1] PLP1 환자와 건강한 섬유아세포 모두에서 Seahorse XF 세포외 분석기로 미토콘드리아 호흡을 측정하고 초고해상도 현미경(회전 디스크 핀홀 기반 구조 조명 현미경, SD-SIM)으로 ER과 미토콘드리아 사이의 상호 작용을 조사했습니다. [2] nan [3] nan [4] nan [5] nan [6] nan [7] nan [8] nan [9] nan [10] nan [11] nan [12] nan [13]
live cell super 라이브 셀 슈퍼
Despite its wide application in live-cell super-resolution (SR) imaging, structured illumination microscopy (SIM) suffers from aberrations caused by various sources. [1] Structured illumination microscopy (SIM) has emerged as an essential technique for three-dimensional (3D) and live-cell super-resolution imaging. [2] Despite its wide application in live-cell super-resolution (SR) imaging, structured illumination microscopy (SIM) suffers from aberrations caused by various sources. [3] describe polarization structured illumination microscopy (pSIM), which combines the benefits of structured illumination for live-cell super-resolution imaging with detailed orientation mapping, giving richer insight into the organization of labeled structures. [4] Structured illumination microscopy (SIM) enables live-cell super-resolution imaging of subcellular structures at high speeds. [5] Using live-cell super-resolution structured illumination microscopy and photobleaching perturbations, we reveal that MRGs undergo fusion and rapidly exchange components, consistent with liquid-liquid phase separation (LLPS). [6]라이브 셀 초해상도(SR) 이미징에 광범위하게 적용되고 있음에도 불구하고 SIM(Structured Illumination Microscopy)은 다양한 소스로 인한 수차로 어려움을 겪고 있습니다. [1] 구조 조명 현미경(SIM)은 3차원(3D) 및 라이브 셀 초해상도 이미징을 위한 필수 기술로 등장했습니다. [2] nan [3] nan [4] nan [5] nan [6]
super resolution technique 초해상도 기술
Structured illumination microscopy (SIM) is another promising super-resolution technique. [1] Images were obtained using three super resolution techniques - atomic force microscopy (AFM), scanning electron microscopy (SEM), and structured illumination microscopy (SIM). [2] Among the various super resolution techniques, Structured Illumination Microscopy (SIM) improve resolution by employing multiple illumination patterns to be deconvolved with a dedicated software. [3] Here, we show that structured illumination microscopy (SIM), a super-resolution technique, can be used to perform super-resolution MFM. [4] Using human fibroblasts as an example, we will highlight several characteristics of telomeres that can be investigated using three different microscopy systems, including wide-field microscopy, and the two super-resolution techniques called 3D Structured Illumination Microscopy (3D-SIM) and direct Stochastic Optical Reconstruction Microscopy (dSTORM). [5] Structured illumination microscopy (SIM) is a promising super-resolution technique for imaging subcellular structures and dynamics due to its compatibility with most commonly used fluorescent label. [6]구조 조명 현미경(SIM)은 또 다른 유망한 초해상도 기술입니다. [1] 이미지는 원자간력현미경(AFM), 주사전자현미경(SEM), 구조조명현미경(SIM)의 세 가지 초해상도 기술을 사용하여 얻었습니다. [2] nan [3] nan [4] nan [5] nan [6]
total internal reflection 내부 전반사
Here, we present Supercritical Illumination Microscopy Photometric z-Localization with Enhanced Resolution (SIMPLER), a photometric method to decode the axial position of single molecules in a total internal reflection fluorescence microscope. [1] In this review, we describe and provide examples of applications of a vast gamut of microscopy techniques, such as widefield fluorescence, total internal reflection fluorescence, laser scanning confocal microscopy, multipoint/slit confocal microscopy, two-photon excited fluorescence (TPEF), second and third harmonic generation (SHG, THG), coherent anti-Stokes Raman scattering (CARS), fluorescence lifetime imaging microscopy (FLIM), structured illumination microscopy (SIM), stimulated emission depletion microscopy (STED), ground-state depletion microscopy (GSD), and photoactivated localization microscopy (PALM/fPALM), as well as their main advantages, limitations. [2] From this point of view, Structured Illumination Microscopy (SIM), Axial Tomography, Total Internal Reflection Fluorescence Microscopy (TIRFM) and often a combination of these methods are used. [3] Here, we improve >2-fold spatially and >10-fold temporally the resolution of planar cellular force probing compared to its related conventional modalities by combining fast two-dimensional total internal reflection fluorescence super-resolution structured illumination microscopy and traction force microscopy. [4] Here, we present three novel TFM approaches that, in combination with total internal reflection, structured illumination microscopy and astigmatism, improve the spatial and temporal performance in either two-dimensional or three-dimensional mechanical force quantification, while maintaining low illumination powers. [5] Our method was also verified by super-resolving the diffraction-limited total internal reflection fluorescence (TIRF) microscopy images, matching the resolution of TIRF-SIM (structured illumination microscopy) images of the same samples, which revealed endocytic protein dynamics in SUM159 cells and amnioserosa tissues of a Drosophila embryo. [6]여기에서는 내부 전반사 형광 현미경에서 단일 분자의 축 위치를 해독하는 측광 방법인 향상된 해상도(SIMPLER)가 있는 초임계 조명 현미경 광도 z-국소화를 제시합니다. [1] 이 리뷰에서 우리는 광시야 형광, 내부 전반사 형광, 레이저 스캐닝 공초점 현미경, 다점/슬릿 공초점 현미경, 2광자 여기 형광(TPEF), 두 번째와 같은 광범위한 현미경 기술의 적용 사례를 설명하고 제공합니다. 및 3차 고조파 발생(SHG, THG), 간섭성 안티-스토크스 라만 산란(CARS), 형광 수명 이미징 현미경(FLIM), 구조 조명 현미경(SIM), 유도 방출 공핍 현미경(STED), 기저 상태 공핍 현미경(GSD) ), 광활성화 국소화 현미경(PALM/fPALM) 및 주요 장점, 한계. [2] nan [3] nan [4] nan [5] nan [6]
three dimensional structured 입체 구조
Three-dimensional structured illumination microscopy (3D-SIM) is an essential tool for volumetric fluorescence imaging, which improves both axial and lateral resolution by down-modulating high-frequency information of the sample into the passband of optical transfer function (OTF). [1] Sequential pulsing with different fluorescent HaloTag ligands in living cells (mouse XX embryonic stem cells engineered with doxycycline-inducible Xist RNA) enabled us to image temporally resolved single Xist RNA molecules using super-resolution three-dimensional structured illumination microscopy (3D-SIM). [2] The three-dimensional structured illumination microscopy (3D-SIM) analyses revealed substantial increases in the number of presented PD-L1 molecules on the cell surface after high LET carbon-ion irradiation compared with X-ray irradiation. [3] Using three-dimensional structured illumination microscopy, we observe that the mitochondrial protein Mitofusin-2 (Mfn2) co-localizes at the plasma membrane with VE-cadherin and β-catenin in endothelial cells during homeostasis. [4] Super-resolution three-dimensional Structured Illumination Microscopy (3D-SIM) is a promising tool to overcome this hurdle and reveal the molecular details of the process of germination of Bacillus subtilis (B. [5]3차원 구조 조명 현미경(3D-SIM)은 시료의 고주파 정보를 광학 전달 함수(OTF)의 통과 대역으로 하향 변조하여 축 및 측면 분해능을 모두 향상시키는 체적 형광 이미징을 위한 필수 도구입니다. [1] 살아있는 세포(doxycycline-inducible Xist RNA로 조작된 마우스 XX 배아 줄기 세포)에서 다른 형광 HaloTag 리간드를 사용한 순차적 펄스를 통해 초고해상도 3차원 구조 조명 현미경(3D-SIM)을 사용하여 일시적으로 해결된 단일 Xist RNA 분자를 이미지화할 수 있었습니다. [2] nan [3] nan [4] nan [5]
live cell imaging 살아있는 세포 이미징
Existing structured illumination microscopy (SIM) allows super-resolution live-cell imaging in few color channels that provide merely morphological information but cannot acquire the sample spectrum that is strongly relevant to the underlying physicochemical property. [1] In this study, we performed live cell imaging with wide-field fluorescence microscopy as well as 3D structured illumination microscopy (3D-SIM) of the core components of LinEs (Rec10, Rec25, Rec27, Mug20) and a linE-binding protein Hop1. [2] Structured illumination microscopy (SIM) has attracted considerable interest in super-resolution, live-cell imaging because of its low light dose and high imaging speed. [3] Supplemental document Multi-color structured illumination microscopy for live cell imaging based on the enhanced image recombination transform algorithm. [4] Using 3D-Structured Illumination Microscopy (3D-SIM) and live cell imaging we show that in fly neural stem cells (neuroblasts) the mitotic kinase Polo and its centriolar protein substrate Centrobin (Cnb) dynamically relocalize from the mother to the daughter centriole during mitosis. [5]기존의 구조적 조명 현미경(SIM)은 형태학적 정보만 제공하지만 근본적인 물리화학적 특성과 밀접한 관련이 있는 샘플 스펙트럼을 획득할 수 없는 소수의 색상 채널에서 초해상도 라이브 셀 이미징을 허용합니다. [1] 이 연구에서 우리는 LineE(Rec10, Rec25, Rec27, Mug20)의 핵심 구성 요소와 lineE 결합 단백질 Hop1의 광시야 형광 현미경과 3D 구조 조명 현미경(3D-SIM)을 사용하여 라이브 세포 이미징을 수행했습니다. [2] nan [3] nan [4] nan [5]
super resolution imaging 초고해상도 이미징
Current mainstream super-resolution imaging technologies can be classified into three types: structured illumination microscopy (SIM), stimulated emission depletion (STED), and single-molecule localization microscopy (SMLM). [1] DTZ-TPA-DCN could be used for the super-resolution imaging of LDs with the structured illumination microscopy. [2] Because structured illumination microscopy (SIM) has the advantages of wide-field, rapid imaging, and biocompatibility, it is widely used for super-resolution imaging of living cells. [3] Recently, super-resolution imaging techniques such as N-SIM (Nikon, Structured Illumination Microscopy) are widely used in cell biology study, allowing cell biologists to obtain unattainable details and relationships of cell structures and functions by conventional confocal imaging. [4] We achieve these gains via an integrated, four-pronged approach: 1) developing compact line-scanners that enable sensitive, rapid, diffraction-limited imaging over large areas; 2) combining line-scanning with multiview imaging, developing reconstruction algorithms that improve resolution isotropy and recover signal otherwise lost to scattering; 3) adapting techniques from structured illumination microscopy, achieving super-resolution imaging in densely labeled, thick samples; 4) synergizing deep learning with these advances, further improving imaging speed, resolution and duration. [5]현재 주류 초해상도 영상 기술은 구조 조명 현미경(SIM), 유도 방출 공핍(STED) 및 단일 분자 국소화 현미경(SMLM)의 세 가지 유형으로 분류할 수 있습니다. [1] DTZ-TPA-DCN은 구조 조명 현미경으로 LD의 초고해상도 이미징에 사용할 수 있습니다. [2] nan [3] nan [4] nan [5]
quantitative oblique back 양적 경사 등
Here we demonstrate that quantitative oblique back illumination microscopy (qOBM)-a novel label-free optical imaging technique that achieves tomographic quantitative phase imaging in thick scattering samples-clearly differentiates between healthy brain tissue and tumor, including infiltrative disease. [1] In this work, we show that quantitative oblique back illumination microscopy (qOBM), a novel label-free optical imaging technique that achieves tomographic quantitative phase imaging (QPI) in thick scattering samples, clearly differentiates between tumor and healthy tissue. [2] Here we describe quantitative oblique back illumination microscopy (qOBM), which overcomes this significant limitation and achieves epiillumination quantitative phase imaging and 3D RI tomography in thick samples, including intact thick tissues. [3] Our approach is based on quantitative oblique back-illumination microscopy (qOBM), which keeps the advantages of QPI—label-free and non-destructive with nanometer-scale sensitivity—while also delivering tomographic sectioning capabilities in thick scattering samples using epi-illumination. [4] This approach leverages a solution to the inverse scattering problem via the general non-paraxial 3D optical transfer function of our quantitative oblique back-illumination microscopy (qOBM) optical system. [5]여기에서 우리는 두꺼운 산란 샘플에서 단층 촬영 정량 위상 이미징을 달성하는 새로운 라벨 없는 광학 이미징 기술인 양적 경사 역광 현미경(qOBM)이 침윤성 질환을 포함하여 건강한 뇌 조직과 종양을 명확하게 구별한다는 것을 보여줍니다. [1] 이 작업에서 우리는 두꺼운 산란 샘플에서 단층 정량 위상 이미징(QPI)을 달성하는 새로운 라벨 없는 광학 이미징 기술인 정량적 경사 역광 현미경(qOBM)이 종양과 건강한 조직을 명확하게 구별한다는 것을 보여줍니다. [2] nan [3] nan [4] nan [5]
confocal laser scanning 공초점 레이저 스캐닝
Slides were imaged using confocal laser scanning, as well as 3D structured illumination microscopy. [1] Functional assays, such as high-content imaging, 3D-structured illumination microscopy (3D-SIM) imaging, flow cytometry, and confocal laser scanning microscopy, were performed to examine the proliferation and lung cancer cell apoptosis. [2] Given the essential role of the cortical microtubules in cell elongation, their organization and dynamics were characterized under the conditions of altered strigolactone signaling using fluorescence microscopy methods with different spatiotemporal capacities, such as confocal laser scanning microscopy (CLSM) and structured illumination microscopy (SIM). [3] As a first application, we super-resolve spectral images of retinal tissue imaged with confocal laser scanning microscopy, by using spatial information from structured illumination microscopy. [4]슬라이드는 공초점 레이저 스캐닝과 3D 구조 조명 현미경을 사용하여 이미지화되었습니다. [1] 고함량 이미징, 3D-SIM(3D-structured illumination microscopy) 이미징, 유세포 분석 및 공초점 레이저 스캐닝 현미경과 같은 기능 분석을 수행하여 증식 및 폐암 세포 사멸을 조사했습니다. [2] nan [3] nan [4]
optical sectioning capability 광학 절편 기능
With sub-diffraction resolution in three dimensions and good optical sectioning capability, three-dimensional superresolution structured illumination microscopy (3D-SRSIM) can provide eight-fold more information than conventional widefield microscopy. [1] In addition, by applying post-processing for structured illumination microscopy to TM-ML-TF microscopy, the optical sectioning capability and the signal-to-background ratio were further enhanced by factors of 1. [2] Structured illumination microscopy (SIM), is a wide-field, minimally-invasive super-resolution optical imaging approach with optical sectioning capability, and it has been extensively applied to many different fields. [3] Optically-sectioned structured illumination microscopy (OS-SIM) is broadly used for biological imaging and engineering surface measurement owing to its simple, low-cost, scanning-free experimental setup and excellent optical sectioning capability. [4]3차원의 하위 회절 분해능과 우수한 광학 단면 기능을 갖춘 3차원 초해상도 구조 조명 현미경(3D-SRSIM)은 기존의 광시야 현미경보다 8배 더 많은 정보를 제공할 수 있습니다. [1] 또한, TM-ML-TF 현미경에 구조 조명 현미경을 위한 후처리를 적용하여 광학 단면 기능과 신호 대 배경 비율을 1배 더 향상시켰습니다. [2] nan [3] nan [4]
stimulated emission depletion 유도 방출 고갈
3D structured illumination microscopy (SIM) imaging of isolated egg cortices demonstrated the graded distribution of Dsh in the VCD, whereas higher resolution stimulated emission depletion (STED) imaging revealed that some individual Dsh puncta consisted of more than one fluorescent source. [1] Within the last decade, several approaches, such as structured illumination microscopy (SIM), stimulated emission depletion STED and (direct) stochastic optical reconstruction microscopy (d)STORM have been established to bypass the diffraction limit. [2] In this thesis, I will present several ExM variants I developed which show the combination of ExM with confocal microscopy, SIM (Structured Illumination Microscopy), STED (STimulated Emission Depletion) and dSTORM. [3] We cover experiment planning and specimen preparation and explain structured illumination microscopy, super-resolution radial fluctuations, stimulated emission depletion microscopy, single-molecule localization microscopy, and super-resolution imaging by pixel reassignment. [4]격리된 계란 피질의 3D 구조화 조명 현미경 검사법(SIM) 이미징은 VCD에서 Dsh의 등급 분포를 시연한 반면, 고해상도 STED(Stimulated Emission Depletion) 이미징은 일부 개별 Dsh puncta가 둘 이상의 형광 소스로 구성되어 있음을 보여주었습니다. [1] 지난 10년 동안 구조 조명 현미경(SIM), 유도 방출 고갈 STED 및 (직접) 확률론적 광학 재구성 현미경(d)STORM과 같은 여러 접근 방식이 회절 한계를 우회하기 위해 확립되었습니다. [2] nan [3] nan [4]
light sheet microscopy 가벼운 시트 현미경
We show that cells can be directly grown on BIO-133 substrates without the need for surface passivation and use this capability to perform extended time-lapse volumetric imaging of cellular dynamics 1) at isotropic resolution using dual-view light-sheet microscopy, and 2) at super-resolution using instant structured illumination microscopy. [1] A combination of fluorescently labeled Fab probes synthesized from these antibodies and light-sheet microscopy, such as dual-view inverted selective plane illumination microscopy (diSPIM), reveal rapid turnover of espin within long-lived F-actin cores of inner-ear sensory hair cell stereocilia, demonstrating that fast-dissociating specific antibodies can identify novel biological phenomena. [2] NEW METHOD New methods are introduced for traumatizing neurons before imaging them with high speed structured illumination microscopy or lattice light sheet microscopy. [3]우리는 세포가 표면 패시베이션 없이 BIO-133 기판에서 직접 성장할 수 있음을 보여주고 이 기능을 사용하여 세포 역학 1) 듀얼 뷰 라이트 시트 현미경을 사용하여 등방성 해상도에서 확장된 시간 경과 체적 이미징을 수행하고 2 ) 즉석 구조 조명 현미경을 사용하여 초해상도에서. [1] 이중 보기 역선택 평면 조명 현미경(diSPIM)과 같은 광시트 현미경과 이러한 항체에서 합성된 형광 표지된 Fab 프로브의 조합은 내이 감각 모발의 수명이 긴 F-액틴 코어 내에서 에스핀의 빠른 회전율을 나타냅니다. 세포 입체 섬모, 빠르게 해리되는 특정 항체가 새로운 생물학적 현상을 식별할 수 있음을 보여줍니다. [2] nan [3]
stochastic optical reconstruction 확률적 광학 재구성
To observe the specific structure and dynamical processes of life activity within living cells more clearly, some SR fluorescence microscopic imaging methods were proposed in the early 21st century, including photoactivated localization microscopy, stochastic optical reconstruction microscopy, stimulated emission depletion microscopy, and structured illumination microscopy (SIM) [10–13] These SR imaging methods extend the resolution limits of microscopy, allowing observation of delicate structures such as the clathrin-coated pits, cytoskeleton of actin and microtubulin, and so forth. [1] The gmSRRF algorithm resolves finer structures and compensates for the loss of resolution caused by artifacts in SRRF images using relatively high-density stochastic optical reconstruction microscopy (STORM) data and conventional widefield, confocal, or structured illumination microscopy (SIM) imaging sequences. [2] ProMyelocytic Leukemia Nuclear Bodies (PML NBs) are distinct dynamic nuclear substructures (approx� 1 micron in diameter) implicated in different physiological and pathological cellular processes, including virus infection� While large viruses, e�g� herpesviruses cause their disruption, smaller DNA viruses, as papillomaor polyomaviruses, realize parts of the reproduction cycle in their close proximity� Previously, we found that Mouse polyomavirus (MPyV) infection causes multiplication and enlargement of PML NBs� During late phases of infection, the integrity and morphology of PML NBs are visibly altered� In addition, we observed the accumulation of MPyV virions around and inside of PML NBs� The aims of our research are: 1� To find whether replication of MPyV genomes itself or rather assembly of virions is responsible for altering the integrity and morphology of PML NBs� 2� To reveal the process of multiplication of PML NBs in infected cells� 3� To visualize the interaction of viral structural and regulatory proteins with PML NBs� We found that replication of mutated MPyV, capable of genome replication and production of all regulatory proteins, is sufficient to alter the morphology of PML NBs, althought it lacks ability to produce structural proteins� Live cell microscopy revealed that in infected cells, PML NBs are highly dynamic structures that assemble from soluble PML NBs’ proteins as well as by fusion or fission of pre-existing nuclear bodies� Using structured illumination microscopy (SIM) and stochastic optical reconstruction microscopy (STORM), we observed the major structural protein of MPyV VP1, to be located inside PML NBs, while the regulatory large T antigen ( bound to replicating MPyV genomes) was located by the surface of PML NBs�. [3]살아있는 세포 내에서 생명 활동의 특정 구조와 역학 과정을 보다 명확하게 관찰하기 위해 21세기 초에 광활성화 국소화 현미경, 확률론적 광학 재구성 현미경, 유도 방출 공핍 현미경 및 구조 조명 현미경을 포함한 일부 SR 형광 현미경 이미징 방법이 제안되었습니다. (SIM) [10-13] 이러한 SR 이미징 방법은 현미경의 해상도 한계를 확장하여 클라트린으로 코팅된 구덩이, 액틴 및 미세소관의 세포골격 등과 같은 섬세한 구조를 관찰할 수 있습니다. [1] gmSRRF 알고리즘은 비교적 고밀도의 확률론적 광학 재구성 현미경(STORM) 데이터와 기존의 광시야, 공초점 또는 구조화된 조명 현미경(SIM) 이미징 시퀀스를 사용하여 SRRF 이미지의 아티팩트로 인해 더 미세한 구조를 해결하고 해상도 손실을 보상합니다. [2] nan [3]
optical super resolution 광학 초해상도
The structured illumination microscopy (SIM) uses standing-wave illumination to reach optical super-resolution. [1] Structured illumination microscopy (SIM) is one of the most powerful and versatile optical super-resolution techniques. [2] Structured illumination microscopy (SIM) has become an important technique for optical super-resolution imaging because it allows a doubling of image resolution at speeds compatible with live-cell imaging. [3]구조 조명 현미경(SIM)은 광학적 초해상도에 도달하기 위해 정상파 조명을 사용합니다. [1] 구조 조명 현미경(SIM)은 가장 강력하고 다양한 광학 초해상도 기술 중 하나입니다. [2] nan [3]
meeting issue ‘ 회의 문제 '
This article is part of the Theo Murphy meeting issue ‘Super-resolution structured illumination microscopy (part 1)'. [1] This article is part of the Theo Murphy meeting issue ‘Super-resolution structured illumination microscopy (part 1)’. [2] This article is part of the Theo Murphy meeting issue ‘Super-resolution structured illumination microscopy (part 1)’. [3]이 기사는 Theo Murphy 회의 호 '초고해상도 구조 조명 현미경(1부)'의 일부입니다. [1] 이 기사는 Theo Murphy 회의 호 '초고해상도 구조 조명 현미경(파트 1)'의 일부입니다. [2] nan [3]
scanning confocal microscopy 주사 공초점 현미경
Laser-scanning confocal microscopy extends the resolution to the nanoscale, allowing us to ultimately image individual liver sinusoidal endothelial cells and their fenestrations by super-resolution structured illumination microscopy. [1] The immunofluorescence assay was imaged using laser scanning confocal microscopy and super-resolution structured illumination microscopy. [2]레이저 스캐닝 공초점 현미경은 해상도를 나노 스케일로 확장하여 궁극적으로 초고해상도 구조 조명 현미경으로 개별 간 정현파 내피 세포와 그 구멍을 이미지화할 수 있습니다. [1] nan [2]
scanning electron microscopy 주사 전자 현미경
To investigate these, we use transgenic tagging in Drosophila flies, 3D-structured illumination microscopy (SIM), and focused ion beam scanning electron microscopy (FIB-SEM) to characterize ERES-Golgi units in collagen-producing fat body, imaginal discs, and imaginal discs overexpressing ERES determinant Tango1. [1] We provide a dynamic 3D view of early secretory compartments in mammalian cells with isotropic resolution and precise protein localization using whole-cell, focused ion beam scanning electron microscopy with cryo-structured illumination microscopy and live-cell synchronized cargo release approaches. [2]이를 조사하기 위해 우리는 초파리 파리, 3D 구조 조명 현미경(SIM) 및 집속 이온빔 주사 전자 현미경(FIB-SEM)에서 유전자 변형 태깅을 사용하여 콜라겐 생성 지방체, 상상 디스크 및 ERES 결정 인자 Tango1을 과발현하는 가상 디스크. [1] 우리는 극저온 구조 조명 현미경 및 라이브 셀 동기화 화물 릴리스 접근 방식을 사용하여 전체 세포, 집속 이온 빔 주사 전자 현미경을 사용하여 등방성 해상도 및 정확한 단백질 위치를 가진 포유동물 세포의 초기 분비 구획의 동적 3D 보기를 제공합니다. [2]
direct stochastic optical
We have used direct stochastic optical reconstruction microscopy (dSTORM) and structured illumination microscopy (SIM) to visualize directly the effect of cellular prion protein (PrP) and two other putative receptors on Ab aggregation process. [1]single molecule localization
We review quantitative approaches to analyze the imaging data of the nuclear lamina as acquired by structured illumination microscopy (SIM) and single molecule localization microscopy (SMLM), as well as the requisite cell preparation techniques. [1]laser scanning microscopy 레이저 스캐닝 현미경
Methods: RPE flatmounts of fifteen human donors were examined using high-resolution structured illumination microscopy (HR-SIM) and laser scanning microscopy (LSM). [1]방법: 15명의 인간 기증자의 RPE 플랫마운트를 고해상도 구조 조명 현미경(HR-SIM)과 레이저 주사 현미경(LSM)을 사용하여 검사했습니다. [1]
Structured Illumination Microscopy 구조 조명 현미경
Three-dimensional structured illumination microscopy (3D-SIM) is an essential tool for volumetric fluorescence imaging, which improves both axial and lateral resolution by down-modulating high-frequency information of the sample into the passband of optical transfer function (OTF). [1] The distinct fluorescent nature of various cNDI monomers aids the spectroscopic probing of the seeded growth process and the microscopic visualization of resultant supramolecular BCPs using Structured Illumination Microscopy (SIM). [2] In this study, we applied for the first time structured illumination microscopy (SIM) to PIAS ligases to investigate the co-localization of PIAS1 and PIAS3 with synaptic markers in hippocampal and cortical murine neurons. [3] To overcome this problem, structured illumination microscopy (SIM) was proposed as a wide-field, optical-sectioning technique, which needs multiple raw images for image reconstruction and thus has a lower imaging speed. [4] Here, we applied Structured Illumination Microscopy (SIM), which improves resolution two-fold over confocal or widefield imaging, to explore the dynamic behaviors of CESA particles in living plant cells. [5] Current mainstream super-resolution imaging technologies can be classified into three types: structured illumination microscopy (SIM), stimulated emission depletion (STED), and single-molecule localization microscopy (SMLM). [6] To observe the specific structure and dynamical processes of life activity within living cells more clearly, some SR fluorescence microscopic imaging methods were proposed in the early 21st century, including photoactivated localization microscopy, stochastic optical reconstruction microscopy, stimulated emission depletion microscopy, and structured illumination microscopy (SIM) [10–13] These SR imaging methods extend the resolution limits of microscopy, allowing observation of delicate structures such as the clathrin-coated pits, cytoskeleton of actin and microtubulin, and so forth. [7] Specifically, this includes whole cell and tissue imaging using a 4Pi single molecule localisation microscope, which uses dual opposing objective lenses and two deformable mirrors for improved z resolution; structured illumination microscopy, with adaptive illumination and aberration correction; and STED-fluorescence correlation spectroscopy in living cells. [8] Structured Illumination Microscopy (SIM) can be used to generate three-dimensional super-resolution (SR) imaging of chromatin by changing in phase and in orientation a periodic line illumination pattern. [9] Here, using super resolution structured illumination microscopy, we find that neither CHMP4B nor CHMP2B are increased in ALS neuronal nuclei. [10] In this study, we developed a technique that allows recapitulation of bristle actin module organization using the Drosophila ovary by a combination of confocal microscopy, super-resolution structured illumination microscopy, and correlative light and electron microscope analysis. [11] Structured illumination microscopy (SIM) imaging on live Bacillus cereus confirmed the suitability of the probe for super-resolution microscopy. [12] Using super‐resolution 3D‐structured illumination microscopy, we observed a spatially restricted up‐regulation of the tight junction protein claudin‐5 (CLDN5) in areas where podocyte processes of patients suffering from minimal change disease (MCD), focal and segmental glomerulosclerosis (FSGS) as well as in murine nephrotoxic serum (NTS) nephritis and uninephrectomy DOCA‐salt hypertension models, were locally injured. [13] Polyplexes consisting of labeled block copolymer with 20 kg mol-1 of P(MEO9 MA) and pDNA are incubated in Hela cells and investigated through structured illumination microscopy (SIM). [14] Super-resolution structured illumination microscopy (SIM) routinely performs image reconstruction in the frequency domain using an approach termed frequency-domain reconstruction (FDR). [15] This article presents answers to the questions on superresolution and structured illumination microscopy as raised in the editorial of a recent publication [K. [16] Structured illumination microscopy (SIM) is another promising super-resolution technique. [17] Here, we utilized the Lattice structured illumination microscopy (Lattice SIM) to visualize Best1 expression at the perisynaptic junctions of the tripartite synapses in CA1 of mouse hippocampus. [18] With sub-diffraction resolution in three dimensions and good optical sectioning capability, three-dimensional superresolution structured illumination microscopy (3D-SRSIM) can provide eight-fold more information than conventional widefield microscopy. [19] Structured Illumination microscopy (SIM) and spectroscopic analyses provide structural characterization of these supramolecular BCPs, which offers various possibilities as axial organic heterostructures. [20] Using flow cytometry, structured illumination microscopy and electron microscopy, we showed that circulating SCD red blood cells abnormally retained their mitochondria, and thus likely to be the source of the elevated cf-mtDNA in SCD patients. [21] Here, we use optical sectioning super-resolution structured illumination microscopy (OS-SR-SIM) with a low magnification objective. [22] By adding consumer-grade available open-source hardware such as digital mirror devices (DMD) and laser projectors we demonstrate a compact 3D multimodal setup that combines image scanning microscopy (ISM) and structured illumination microscopy (SIM). [23] Structured Illumination Microscopy (SIM) is a widespread methodology to image live and fixed biological structures smaller than the diffraction limits of conventional optical microscopy. [24] Our method generates images with a significantly improved SNR, compared to wide-field structured illumination microscopy (WF-SIM), without residual modulation artifacts. [25] We present a structured illumination microscopy system that projects a hexagonal pattern by the interference among three coherent beams, suitable for implementation in a light-sheet geometry. [26] In this chapter, we describe a method for live imaging of mitochondria and nucleoids in differentiated SH-SY5Y cells by instant structured illumination microscopy (iSIM). [27] In this paper, tilt illumination mode is introduced to structured illumination microscopy (SIM) for enhancing lateral resolution. [28] Existing structured illumination microscopy (SIM) allows super-resolution live-cell imaging in few color channels that provide merely morphological information but cannot acquire the sample spectrum that is strongly relevant to the underlying physicochemical property. [29] Through high-resolution 3D-structured illumination microscopy and functional analyses, we report multiple biological processes associated with the meiosis-specific cohesin components, REC8 and STAG3, and the distinct loss of function of meiotic cohesin during the cell cycle of embryonic stem cells (ESCs). [30] In this chapter, we describe a single-molecule microscopy approach that combines fluorescent RNA in situ hybridization (smFISH) and structured illumination microscopy (SIM ) and allows to measure different aspects of RNP organization in cells, including distances between different regions within individual mRNAs, as well as the overall compaction state of RNAs in different subcellular compartments and environmental conditions. [31] We tested our methods on four increasingly challenging samples including tissue, in which case results were comparable to the ones obtained by structured illumination microscopy in terms of contrast. [32] To understand how NPC density and organization is controlled, we analyzed NPC number and distribution in the fission yeast Schizosaccharomyces pombe using structured illumination microscopy. [33] Methods Immunocytochemistry including structured illumination microscopy and immunoblotting was used to determine expression levels of contactin-1 and/or sodium channels after long-term exposure to autoantibodies from 3 seropositive patients. [34] Images were obtained using three super resolution techniques - atomic force microscopy (AFM), scanning electron microscopy (SEM), and structured illumination microscopy (SIM). [35] Furthermore, structured illumination microscopy (SIM) offers a unique possibility to go below the optical diffraction limit while simultaneously operating and acquiring AFM images. [36] Despite its wide application in live-cell super-resolution (SR) imaging, structured illumination microscopy (SIM) suffers from aberrations caused by various sources. [37] DTZ-TPA-DCN could be used for the super-resolution imaging of LDs with the structured illumination microscopy. [38] Structured illumination microscopy (SIM) is a widely used imaging technique that doubles the effective resolution of widefield microscopes. [39] We have shown using kinetics and structured illumination microscopy that: (a) efflux active P-gp is controlled by microvilli morphology; (b) there are apical (AT) and basolateral (BT) uptake transporters for P-gp substrates in most, if not all, P-gp expressing cell lines used in the pharmaceutical industry, which exist, but which remain unidentified; (c) the lab-to-lab variability in P-gp IC50 values observed in the P-gp IC50 initiative was due to the conflated inhibition of P-gp and the basolateral digoxin uptake transporters by all 15 P-gp substrates tested in that study; (d) even the IC50 values for P-gp inhibition alone do not obey the Cheng-Prusoff relationship; (e) the fitted elementary rate constants and the molecular dissociation constant Ki for this kinetic model are system independent; and (f) the time dependence of product formation for these confluent cell monolayers is correlated with the P-gp Vmax/Km, when defined by its fitted elementary rate constants and uptake transporter clearances, without any steady-state assumptions. [40] Three-dimensional (3D) structured illumination microscopy (SIM) plays an important role in biological volumetric imaging with the capabilities of doubling the lateral and axial resolution and optical sectioning. [41] Using super-resolution 3D structured illumination microscopy, we observed a spatially restricted up-regulation of the tight junction protein claudin 5 (CLDN5) in areas where podocyte processes of patients suffering from minimal change disease (MCD), focal and segmental glomerulosclerosis (FSGS) as well as in murine nephrotoxic serum (NTS) nephritis and uninephrectomy DOCA-salt hypertension models, were locally injured. [42] We significantly improve signal to noise ratio and throughput compared to wild-filed structured illumination microscopy. [43] Herein, we propose a “landmine warfare strategy”, probe L-1 evenly “buries” in the cellular matrix as a “landmine” (no fluorescence), when ONOO– “stepped on” the probe L-l, causing the “landmine explosion” to release fluorophore, thus capturing ONOO– particles, under structured illumination microscopy (SIM). [44] Applying instantaneous structured illumination microscopy and stimulated emission double depletion microscopy to pluripotent zebrafish embryos, we find recruited Pol II associated with large clusters, and elongating Pol II with dispersed clusters. [45] Laser-scanning confocal microscopy extends the resolution to the nanoscale, allowing us to ultimately image individual liver sinusoidal endothelial cells and their fenestrations by super-resolution structured illumination microscopy. [46] In a tunable 3D structured illumination microscopy (3D-SIM) based on an illumination system comprised by a multi-slit array and a Fresnel biprism, the 3D structured illumination (SI) pattern depends on the design of the slit array. [47] 3D structured illumination microscopy (SIM) imaging of isolated egg cortices demonstrated the graded distribution of Dsh in the VCD, whereas higher resolution stimulated emission depletion (STED) imaging revealed that some individual Dsh puncta consisted of more than one fluorescent source. [48] Herein, we report a probe LD-FG for imaging lipid droplet (LD) dynamics using structured illumination microscopy (SIM). [49] This identification is reinforced by colocalization of pairwise combinations of DSB-1, DSB-2, and DSB-3 foci in structured illumination microscopy images of spread nuclei. [50]3차원 구조 조명 현미경(3D-SIM)은 시료의 고주파 정보를 광학 전달 함수(OTF)의 통과 대역으로 하향 변조하여 축 및 측면 분해능을 모두 향상시키는 체적 형광 이미징을 위한 필수 도구입니다. [1] 다양한 cNDI 단량체의 독특한 형광 특성은 시드 성장 과정의 분광학적 조사와 구조적 조명 현미경(SIM)을 사용하여 생성된 초분자 BCP의 현미경 시각화를 돕습니다. [2] nan [3] nan [4] nan [5] 현재 주류 초해상도 영상 기술은 구조 조명 현미경(SIM), 유도 방출 공핍(STED) 및 단일 분자 국소화 현미경(SMLM)의 세 가지 유형으로 분류할 수 있습니다. [6] 살아있는 세포 내에서 생명 활동의 특정 구조와 역학 과정을 보다 명확하게 관찰하기 위해 21세기 초에 광활성화 국소화 현미경, 확률론적 광학 재구성 현미경, 유도 방출 공핍 현미경 및 구조 조명 현미경을 포함한 일부 SR 형광 현미경 이미징 방법이 제안되었습니다. (SIM) [10-13] 이러한 SR 이미징 방법은 현미경의 해상도 한계를 확장하여 클라트린으로 코팅된 구덩이, 액틴 및 미세소관의 세포골격 등과 같은 섬세한 구조를 관찰할 수 있습니다. [7] nan [8] nan [9] 여기에서 초해상도 구조 조명 현미경을 사용하여 ALS 신경 핵에서 CHMP4B와 CHMP2B가 모두 증가하지 않는다는 것을 발견했습니다. [10] 이 연구에서 우리는 공초점 현미경, 초해상도 구조 조명 현미경, 상관광 및 전자 현미경 분석을 조합하여 초파리 난소를 사용하여 강모 액틴 모듈 조직을 요약할 수 있는 기술을 개발했습니다. [11] 살아있는 Bacillus cereus에 대한 SIM(Structured Illumination Microscopy) 이미징은 초해상도 현미경에 대한 프로브의 적합성을 확인했습니다. [12] nan [13] nan [14] nan [15] nan [16] 구조 조명 현미경(SIM)은 또 다른 유망한 초해상도 기술입니다. [17] nan [18] 3차원의 하위 회절 분해능과 우수한 광학 단면 기능을 갖춘 3차원 초해상도 구조 조명 현미경(3D-SRSIM)은 기존의 광시야 현미경보다 8배 더 많은 정보를 제공할 수 있습니다. [19] nan [20] nan [21] nan [22] nan [23] nan [24] nan [25] 우리는 3개의 간섭성 빔 사이의 간섭에 의해 육각형 패턴을 투영하는 구조화된 조명 현미경 시스템을 제시하며, 이는 라이트 시트 형상에서 구현하기에 적합합니다. [26] nan [27] nan [28] 기존의 구조적 조명 현미경(SIM)은 형태학적 정보만 제공하지만 근본적인 물리화학적 특성과 밀접한 관련이 있는 샘플 스펙트럼을 획득할 수 없는 소수의 색상 채널에서 초해상도 라이브 셀 이미징을 허용합니다. [29] nan [30] nan [31] nan [32] nan [33] nan [34] 이미지는 원자간력현미경(AFM), 주사전자현미경(SEM), 구조조명현미경(SIM)의 세 가지 초해상도 기술을 사용하여 얻었습니다. [35] nan [36] 라이브 셀 초해상도(SR) 이미징에 광범위하게 적용되고 있음에도 불구하고 SIM(Structured Illumination Microscopy)은 다양한 소스로 인한 수차로 어려움을 겪고 있습니다. [37] DTZ-TPA-DCN은 구조 조명 현미경으로 LD의 초고해상도 이미징에 사용할 수 있습니다. [38] nan [39] nan [40] nan [41] nan [42] nan [43] nan [44] nan [45] 레이저 스캐닝 공초점 현미경은 해상도를 나노 스케일로 확장하여 궁극적으로 초고해상도 구조 조명 현미경으로 개별 간 정현파 내피 세포와 그 구멍을 이미지화할 수 있습니다. [46] nan [47] 격리된 계란 피질의 3D 구조화 조명 현미경 검사법(SIM) 이미징은 VCD에서 Dsh의 등급 분포를 시연한 반면, 고해상도 STED(Stimulated Emission Depletion) 이미징은 일부 개별 Dsh puncta가 둘 이상의 형광 소스로 구성되어 있음을 보여주었습니다. [48] nan [49] 이 식별은 확산 핵의 구조화 조명 현미경 이미지에서 DSB-1, DSB-2 및 DSB-3 초점의 쌍별 조합의 colocalization에 의해 강화됩니다. [50]
Plane Illumination Microscopy 평면 조명 현미경
We call this method local-delivery selective-plane illumination microscopy (ldSPIM). [1] Background Conventional light sheet fluorescence microscopy (LSFM), or selective plane illumination microscopy (SPIM), enables high-resolution 3D imaging over a large volume by using two orthogonally aligned objective lenses to decouple excitation and emission. [2] In this study, we employ a single-color fluorescence anisotropy reporter (FLARE), Venus FLARE-Cameleon, and polarization inverted selective-plane illumination microscopy (piSPIM) to measure rhythmic changes in cytosolic Ca2+ in SCN neurons. [3] Light sheet or selective plane illumination microscopy (SPIM) is ideally suited for in toto imaging of living specimens at high temporal-spatial resolution. [4] This is evident, for example, in selective plane illumination microscopy where acquisition rates of about 1–4 GB/s sustained over several days have redefined the scale of I/O bandwidth required by image analysis tools. [5] We also measured the ventricular volume and monitored the opening/closing activity of the AV and VB valves using 4D selective plane illumination microscopy (SPIM). [6] Highlights • μSPIM Toolset is open-source software for Selective Plane Illumination Microscopy. [7] The invention of tissue clearing, advances in immunohistochemistry and development of selective plane illumination microscopy (SPIM) now make it possible to acquire whole mouse brain images at submicron spatial resolution with a vast array of cell specific markers [10] [11] [12] [13]. [8] We hereby coupled LSFM, also known as selective plane illumination microscopy, with topological quantification, to characterize the retinal vascular plexuses undergoing preferential obliteration. [9] One workflow is based on tissue clearing and selective plane illumination microscopy, whereas the other workflow is based on serial block-face two-photon microscopy. [10] A combination of fluorescently labeled Fab probes synthesized from these antibodies and light-sheet microscopy, such as dual-view inverted selective plane illumination microscopy (diSPIM), reveal rapid turnover of espin within long-lived F-actin cores of inner-ear sensory hair cell stereocilia, demonstrating that fast-dissociating specific antibodies can identify novel biological phenomena. [11] Light sheet or selective plane illumination microscopy (SPIM) is ideally suited for in toto imaging of living specimens at high temporal-spatial resolution. [12] Over the last few years, techniques such as intravital, optoacoustic and magnetic resonance imaging, optical projection tomography, and selective plane illumination microscopy developed promising potential for gaining insights into host-pathogen interactions by allowing different visualization forms in vivo and ex vivo. [13] Hydroxyapatite-stained GG-SPD samples were imaged with Optical Projection Tomography (OPT) and Selective Plane Illumination Microscopy (SPIM) in OM and BaG OM at 21 d. [14] Single Plane Illumination Microscopy (SPIM) revolutionized time lapse imaging of live cells and organisms due to its high speed and reduced photodamage. [15] Tiling light sheet selective plane illumination microscopy (TLS-SPIM) improves the 3D imaging ability of SPIM by using real-time optimized tiling light sheets. [16]우리는 이 방법을 국부 전달 선택적 평면 조명 현미경(ldSPIM)이라고 부릅니다. [1] 배경 기존의 광 시트 형광 현미경(LSFM) 또는 선택적 평면 조명 현미경(SPIM)은 여기와 방출을 분리하기 위해 두 개의 직교 대물 렌즈를 사용하여 대용량에 대한 고해상도 3D 이미징을 가능하게 합니다. [2] nan [3] nan [4] nan [5] nan [6] nan [7] nan [8] nan [9] nan [10] 이중 보기 역선택 평면 조명 현미경(diSPIM)과 같은 광시트 현미경과 이러한 항체에서 합성된 형광 표지된 Fab 프로브의 조합은 내이 감각 모발의 수명이 긴 F-액틴 코어 내에서 에스핀의 빠른 회전율을 나타냅니다. 세포 입체 섬모, 빠르게 해리되는 특정 항체가 새로운 생물학적 현상을 식별할 수 있음을 보여줍니다. [11] nan [12] nan [13] nan [14] nan [15] nan [16]
Back Illumination Microscopy 후면 조명 현미경
Here we demonstrate that quantitative oblique back illumination microscopy (qOBM)-a novel label-free optical imaging technique that achieves tomographic quantitative phase imaging in thick scattering samples-clearly differentiates between healthy brain tissue and tumor, including infiltrative disease. [1] In this work, we show that quantitative oblique back illumination microscopy (qOBM), a novel label-free optical imaging technique that achieves tomographic quantitative phase imaging (QPI) in thick scattering samples, clearly differentiates between tumor and healthy tissue. [2] Here we describe quantitative oblique back illumination microscopy (qOBM), which overcomes this significant limitation and achieves epiillumination quantitative phase imaging and 3D RI tomography in thick samples, including intact thick tissues. [3]여기에서 우리는 두꺼운 산란 샘플에서 단층 촬영 정량 위상 이미징을 달성하는 새로운 라벨 없는 광학 이미징 기술인 양적 경사 역광 현미경(qOBM)이 침윤성 질환을 포함하여 건강한 뇌 조직과 종양을 명확하게 구별한다는 것을 보여줍니다. [1] 이 작업에서 우리는 두꺼운 산란 샘플에서 단층 정량 위상 이미징(QPI)을 달성하는 새로운 라벨 없는 광학 이미징 기술인 정량적 경사 역광 현미경(qOBM)이 종양과 건강한 조직을 명확하게 구별한다는 것을 보여줍니다. [2] nan [3]