Centrifugal Partition
원심 분할

Centrifugal Partition(원심 분할)란 무엇입니까?

Centrifugal Partition 원심 분할 - The present work describes the use of Centrifugal Partition Chromatography (CPC) for the bio-guided isolation of repellent active volatile compounds from essential oils. ^[1] In this study, a centrifugal partition chromatography (CPC) separation was applied to identify antioxidant-responsive element (ARE) induction molecules from the crude extract of Lindera strychnifolia roots. ^[2] The application of centrifugal partition chromatography helped isolate BDP, which was identified by 1H NMR and MS analyses. ^[3] This method is an optimized version of a well-established dereplication procedure and consists of a fractionation by centrifugal partition chromatography followed by 13C nuclear magnetic resonance analysis and dedicated data processing. ^[4] Recently, advanced analytical methods including thin layer chromatography, high-performance thin layer chromatography, gas chromatography, high-performance liquid chromatography, ultra-high performance liquid chromatography with tandem ultraviolet, diode array detector, evaporative light scattering detector, and mass detector, two-dimensional high-performance liquid chromatography, high speed counter-current chromatography, high speed centrifugal partition chromatography, micellar electrokinetic chromatography, high-performance anion-exchange chromatography, ambient ionization mass spectrometry, molecularly imprinted polymer, enzyme immunoassay, 1H-NMR, and infrared spectroscopy have been used to identify and evaluate chemical constituents in Panax species. ^[5] The isolation of two bioactive phenolic compounds verbascoside and forsythoside B from Buddleja globosa (Buddlejaceae) was successfully achieved by centrifugal partition chromatography (CPC). ^[6] A single-step hydrostatic CCS methodology (Centrifugal Partition Chromatography [CPC]) was developed that fractionated the isoflavonoids with a hexanes-ethyl acetate-methanol-water (HEMWat) 5. ^[7] A new extract with a purity of 99% in stilbenes was obtained by centrifugal partition chromatography. ^[8] High-purity BBR was isolated by centrifugal partition chromatography from a methanolic extract from B. ^[9] Separation of the n-BuOH-soluble fraction by centrifugal partition chromatography, followed by preparative and semipreparative HPLC, afforded a series of bidesmosidic glycosides of echinocystic acid bearing a 3,16-dihydroxy-10-oxo-hexadecanoic acid residue attached to the glycosidic moiety at C-28. ^[10] The purpose of this work is to integrate the extraction of anthocyanins from grape pomace with a sequential purification process using an aqueous two-phase system (ATPS), and scaled-up in centrifugal partition chromatography (CPC) using as a common solvent the protic ionic liquids based on ethanolammonium and sulfuric acid. ^[11] 5 wt% Na2SO4 was chosen and applied on the fast centrifugal partition chromatography (FCPC). ^[12] The lab-scale liquid-liquid chromatography separation performed in a countercurrent chromatography (CCC) column was successfully transferred to a semi-preparative centrifugal partition chromatography (CPC) column, which enabled the isolation of artemisia ketone, artemisia alcohol, α-bisabolone oxide A, and (E)-en-yn-dicycloether. ^[13] pinnatum and its closely related species for health purposes, the target metabolite was isolated by a combination of centrifugal partition chromatography in elution/extrusion mode and MPLC in order to confirm its structure. ^[14] Extracts were fractionated by solvent partitioning, high-performance centrifugal partition chromatography (HPCPC), and preparative high-performance liquid chromatography (prep-HPLC). ^[15] This study provides an efficient pilot-scale liquid-liquid extraction process for the recovery of total phenolic fraction (TPF) from extra virgin olive oil (EVOO) and the further purification of the major TPF constituents (oleocanthal, oleacein, monoaldehydic form of oleuropein aglycone, monoaldehydic form of ligstroside aglycone and hydroxytyrosol) using successively centrifugal partition chromatography and reversed phase high performance liquid chromatography. ^[16] The article revisits the discrete recurrence method to model the instruments of liquid–liquid partition chromatography as counter-current chromatography (CCC) and centrifugal partition chromatography (CPC). ^[17] Centrifugal partition chromatography was used to fractionate crude resulting irradiated materials using a biphasic solvent system composed of tert-butyl alcohol - ethyl acetate - water (0. ^[18] For this purpose, a gentle strategy combining pH-zone-refining centrifugal partition chromatography (pH-ZRCPC) and preparative reversed-phase liquid chromatography (Prep-RPLC) was developed to recover one hundred milligrams of a high purity apple flavan-3-ol fraction. ^[19] This system was further employed for the resolution of R/S-PRP in centrifugal partition chromatography, to assess its scalability potential, being shown that it was possible to increase the purity of R-PRP from 59% to 75%. ^[20] To achieve an efficient management of these hydrocarbons, a detailed semi-quantitative description of PAHs contained in VGOs was provided by a three-dimensional off-line analytical approach based on centrifugal partition chromatography (CPC), supercritical fluid chromatography (SFC) and high-resolution mass spectrometry (HRMS). ^[21] The fatty acids released were then recovered by two different methods: one using a centrifugal partition extractor device and the other using a continuous centrifugal extractor device. ^[22] Fractionation of grape seed macerates was achieved by liquid-liquid extraction, centrifugal partition chromatography (CPC) and preparative HPLC. ^[23] This is the first extensive review describing the application of CCS for purification of iridoids and PhGs, based on the research papers from the last 20 years (1998–2018) that used countercurrent chromatography and centrifugal partition chromatography technologies. ^[24] The present study aims to develop a bio-guided fractionation using the Fast-Centrifugal Partition Chromatography (FCPC) to isolate and identify bactericidal compounds against V. ^[25] Centrifugal partition chromatography (CPC) is a well-established technology for natural compound separation. ^[26] The methanolic extract of aerial parts of this plant was processed using fast centrifugal partition chromatography, resulting in isolation of four archetypal isoflavones (genistein, daidzein, isoprunetin, 8-C-β-D-glucopyranosyl-genistein) and ten derivatives thereof. ^[27] In the present study, purification of the six major compounds from a methanol extract of the plant's subaerial parts was achieved by centrifugal partition chromatography. ^[28] Antisperill; Lamiaceae) by centrifugal partition chromatography (CPC). ^[29] The performance of the Centrifugal Partition Chromatography (CPC) as a liquid-liquid chromatographic technique depends strongly on the two-phase solvent system used. ^[30] The extraction of SO phenolic content was studied using two different extraction techniques: Annular centrifugal extraction (ACE) and centrifugal partition extraction (CPE). ^[31] In this study, an innovative centrifugal partition chromatography (CPC) protocol was developed to fractionate aqueous biomass samples with a MTBE-water solvent system, by mixing elution, displacement and extrusion modes in the same run. ^[32] To optimise the method, numerous biphasic solvent systems (chloroform-, acetone- and acetic acid-based systems) were tested and evaluated in terms of their effectiveness at partitioning aflatoxins; the toluene/acetic acid/water (30:24:50, v/v/v/%) system was found to be the most efficient for application in centrifugal partition chromatographic instrument. ^[33] Bioassay-guided fractionation was performed by Fast Centrifugal Partition Chromatography and preparative HPLC, followed by LC-MS and NMR investigations for structure elucidation. ^[34] In the countercurrent chromatography and centrifugal partition chromatography, separation method transfer and scale-up is often described as an easy and straightforward procedure. ^[35] Herein, the effective use of aqueous biphasic systems (ABS) in Fast Centrifugal Partition Chromatography (FCPC) for the purification of PEGylated cytochrome c conjugates is shown. ^[36] The obtained results were further confronted with a classic microdilution antimicrobial assay on the isolates, purified from the crude extracts by centrifugal partition chromatography in the following solvent system: heptane-chloroform-methanol-water (5:6:3:2) (v/v/v/v). ^[37] To isolate original natural products directly from Jacobaea gigantea crude polar extracts, centrifugal partition chromatography (CPC) was used. ^[38] In order to rapidly isolate larger quantities for biological testing, a fast, one‐step isolation method by centrifugal partition chromatography was developed within this study. ^[39] Scale up is possible via centrifugal partition chromatography (CPC) configurations. ^[40] The method development of liquid-liquid chromatography, either countercurrent chromatography or centrifugal partition chromatography, is slowed down by the selection of the biphasic solvent system that constitutes its column. ^[41] To facilitate the screening and the identification of active compounds, the present study describes their targeting by combining the dereplication information obtained by means of Molecular Networks and a crude extract fractionation by Centrifugal Partition Chromatography to obtain and test simplified fractions. ^[42] LLC, also known as countercurrent chromatography (CCC) or centrifugal partition chromatography (CPC), offers the user a variety of operating modes, many of which have no direct equivalent in conventional preparative liquid-solid chromatography. ^[43] This study aimed to establish an efficient process for the extraction of glaucolide B (1) from Lepidaploa chamissonis leaves and to develop a simple and fast method for its purification by using centrifugal partition chromatography (CPC), as well as to investigate in vitro the anti-inflammatory effects of glaucolide B. ^[44] countercurrent chromatography and centrifugal partition chromatography. ^[45] The presentations in the scientific program demonstrated the progress in the field of countercurrent chromatography (CCC) and centrifugal partition chromatography (CPC) in recent years and numerous applications have impressively proven the potential of this all-liquid separation technique not only for academic research but also for industry. ^[46] Recently the Centrifugal Partition Chromatography (CPC) device, called Centrifugal Partition Reactor (CPR), was proposed to operate biocatalytic reactions in two phase systems in continuous mode. ^[47] Five flavones have been isolated by fast centrifugal partition chromatography, followed by preparative HPLC. ^[48] A hydrostatic CCS (also known as centrifugal partition chromatography, CPC) technique was used to enrich and deplete selected bioactive isoflavones of RCE extracts. ^[49]