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Whole Cell Biocatalyst sentence examples within one pot reaction

one pot reaction

Concerning the latter, the two whole-cell biocatalysts were coupled with laccase/TEMPO in a one-pot reaction designed to enable catalysis of the three oxidative steps necessary to convert HMF into 2,5-furandicarboxylic acid (FDCA), a compound with immense potential in the production of sustainable and eco-friendly polymers.

Use of filamentous fungi as biocatalysts in the oxidation of 5-(hydroxymethyl)furfural (HMF).

one pot reaction

Finally, an efficient process of recycling and reusing of SO 4 2− /SnO 2 -bentonite catalyst and immobilized whole-cell biocatalyst was developed for the chemoenzymatic synthesis of furfuryl alcohol from biomass in the one-pot reaction system.

Chemoenzymatic Synthesis of Furfuryl Alcohol from Biomass in Tandem Reaction System

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10.1128/AEM.01335-21

Furthermore, the production of l-threo-β-hydroxy-His and l-threo-β-hydroxy-Gln was achieved using Escherichia coli expressing the gene encoding AEP14369 as a whole-cell biocatalyst.

Enzymatic Synthesis of l-threo-β-Hydroxy-α-Amino Acids via Asymmetric Hydroxylation Using 2-Oxoglutarate-Dependent Hydroxylase from Sulfobacillus thermotolerans Strain Y0017

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10.1007/s12275-021-1023-x

YGSMI21 can be regarded as a whole-cell biocatalyst in the production of various (S)-CSO with the chlorine group at a different position.

Isolation of a novel strain, Sphingorhabdus sp. YGSMI21 and characterization of its enantioselective epoxide hydrolase activity.

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10.1016/J.BEJ.2021.108117

After optimizing the gene dose ratio of mbSUS and UGT76G1, which is an effective whole-cell biocatalyst GS115 (U76/mb3:1) was constructed.

Effective synthesis of Rebaudioside A by whole-cell biocatalyst Pichia pastoris

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10.1016/j.ymben.2021.07.007

Escherichia coli-based whole-cell biocatalysts are widely used for the sustainable production of value-added chemicals.

Enhancing acid tolerance of Escherichia coli via viroporin-mediated export of protons and its application for efficient whole-cell biotransformation.

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10.1186/s12896-021-00715-5

A whole-cell biocatalyst expressing the engineered reductase and a yeast formate dehydrogenase for NADH-recycling provided substantial stabilization of the reductase.

Reductive enzymatic dynamic kinetic resolution affording 115 g/L (S)-2-phenylpropanol

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10.3390/catal11070757

The yield of levan produced by these two whole-cell biocatalysts reaches 26 g/L and 34 g/L in 24 h, respectively.

Development and Characterization of Two Types of Surface Displayed Levansucrases for Levan Biosynthesis

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10.21577/0100-4042.20170747

Furthermore, a summary of recent developments pertaining to the potential applications of decoy molecules from the development of a whole-cell biocatalyst to their use in crystallography will be discussed.

ENGANANDO O CITOCROMO P450BM3: CATÁLISE DE VÁRIAS TRANSFORMAÇÕES DE SUBSTRATOS NÃO NATIVOS USANDO MOLÉCULAS-TRAIÇOEIRAS

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10.1186/s12934-021-01646-8

coli strain that expressed GADZ11 was used as a whole-cell biocatalyst for the production of GABA.

Characterization of three glutamate decarboxylases from Bacillus spp. for efficient γ-aminobutyric acid production

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10.1002/chir.23318

In this study, the reductive capacity of Lactobacillus paracasei BD101 was investigated as whole-cell biocatalyst in the enantioselective reduction of 1-tetralone (1).

Biocatalytic asymmetric synthesis of (R)-1-tetralol using Lactobacillus paracasei BD101.

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10.1016/J.CEJ.2021.128665

In this study, a novel whole-cell biocatalyst that can accelerate the degradation of antibiotics was developed by displaying β-lactamase on the cell surface of Aspergillus niger.

Using Aspergillus niger whole-cell biocatalyst mycelial aerobic granular sludge to treat pharmaceutical wastewater containing β-lactam antibiotics

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10.1186/s40643-021-00370-w

The engineered whole-cell biocatalyst was used in four sequential batches to achieve a production of 126 mM ε-caprolactone with a high molar yield of 0.

Tuning a bi-enzymatic cascade reaction in Escherichia coli to facilitate NADPH regeneration for ε-caprolactone production

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10.1007/s00726-021-03067-8

In our previous study, one-step pyruvate and D-alanine production from D,L-alanine by a whole-cell biocatalyst Escherichia coli expressing L-amino acid deaminase (Pm1) derived from Proteus mirabilis was investigated.

Semi-rational design of L-amino acid deaminase for production of pyruvate and D-alanine by Escherichia coli whole-cell biocatalyst.

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10.1016/J.PROCBIO.2021.07.020

In-Cell Crosslinked Enzymes (InCLEs) is a procedure to improve the thermal and operational stability of Bacillus megaterium intracellular decarboxylase activity, allowing to obtain enhanced whole-cell biocatalyst to produce 4-vinyl guaiacol (4VG).

In-Cell Crosslinked Enzymes: Improving Bacillus megaterium whole-cell biocatalyst stability for the decarboxylation of ferulic acid

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10.1186/s12934-021-01528-z

Background The display of recombinant proteins on cell surfaces has a plethora of applications including vaccine development, screening of peptide libraries, whole-cell biocatalysts and biosensor development for diagnostic, industrial or environmental purposes.

Decorating the surface of Escherichia coli with bacterial lipoproteins: a comparative analysis of different display systems

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10.3390/catal11091038

With respect to biotechnological application, Escherichia coli whole-cell biocatalysts were developed and characterized in small-scale biotransformations.

From Cell-Free Protein Synthesis to Whole-Cell Biotransformation: Screening and Identification of Novel α-Ketoglutarate-Dependent Dioxygenases for Preparative-Scale Synthesis of Hydroxy-l-Lysine

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10.1007/s12010-020-03477-6

coli strain was used as a whole-cell biocatalyst to produce trans-cinnamic acid from l-Phe.

Identification and Characterization of an Efficient Phenylalanine Ammonia-Lyase from Photorhabdus luminescens

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10.1007/s13205-021-02885-7

In this study, we aimed to develop a Bacillus megaterium based whole-cell biocatalyst for the bio-production of 3-aminopropionic acid (3-APA).

Combinatorial approach for improved production of whole-cell 3-aminopropionic acid in recombinant Bacillus megaterium: codon optimization, gene duplication and process optimization

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10.21203/RS.3.RS-183141/V1

A whole-cell biocatalyst based on the engineered reductase and a yeast formate dehydrogenase for NADH-recycling provided substantial stabilization of the reductase.

Enzymatic Dynamic Reductive Kinetic Resolution Towards 115 g/L (S)-2-Phenylpropanol

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10.1016/bs.mie.2020.12.030

This chapter deals with the construction and application of a whole-cell biocatalyst by displaying IsPETase on the surface of yeast (Pichia pastoris) cells.

Yeast cell surface display of bacterial PET hydrolase as a sustainable biocatalyst for the degradation of polyethylene terephthalate.

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10.1186/s40643-021-00438-7

In this study, low-cost sugarcane molasses was used to produce whole-cell biocatalysts containing transfructosylating enzymes by Aureobasidium pullulans FRR 5284, followed by FOS production from molasses using the whole-cells of A.

Transformation of sugarcane molasses into fructooligosaccharides with enhanced prebiotic activity using whole-cell biocatalysts from Aureobasidium pullulans FRR 5284 and an invertase-deficient Saccharomyces cerevisiae 1403-7A

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10.1080/10286020.2021.1981875

Quercetin (1) was converted into quercetin 7-O-succinyl glucoside (2) by used Bacillus amyloliquefaciens FJ18 as a solvent-resistant whole-cell biocatalyst.

Biosynthesis and evaluation of a novel highly water-soluble quercetin glycoside derivative.

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10.1002/anie.202111612

We report an OmpF loop deletion mutant, which improves the cellular uptake of external additives into an Escherichia coli whole-cell biocatalyst.

Designer Outer Membrane Protein Facilitates Uptake of Decoy Molecules into a Cytochrome P450BM3-Based Whole-Cell Biocatalyst.

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10.1007/s00253-021-11463-z

coli into a whole-cell biocatalyst for hydrogen-driven CO2 reduction to formate without the need of any external co-factors or endogenous enzymes in the reaction process.

Capture of carbon dioxide and hydrogen by engineered Escherichia coli: hydrogen-dependent CO2 reduction to formate

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10.1016/j.biortech.2021.126169

Concerning the latter, the two whole-cell biocatalysts were coupled with laccase/TEMPO in a one-pot reaction designed to enable catalysis of the three oxidative steps necessary to convert HMF into 2,5-furandicarboxylic acid (FDCA), a compound with immense potential in the production of sustainable and eco-friendly polymers.

Use of filamentous fungi as biocatalysts in the oxidation of 5-(hydroxymethyl)furfural (HMF).

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10.1016/j.mcat.2020.111341

In this work, a yeast-like fungus, Aureobasidium subglaciale F134 was discovered as a whole-cell biocatalyst for BHMF synthesis from selective reduction of HMF.

Efficient reduction of 5-hydroxymethylfurfural to 2, 5-bis (hydroxymethyl) furan by a fungal whole-cell biocatalyst

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10.21203/RS.3.RS-348238/V1

coli strain that expressed GADZ11 was used as a whole-cell biocatalyst for the production of GABA.

Characterization of Three GADs from Bacillus spp. for Efficient γ-Aminobutyric Acid Production

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10.1002/chir.23337

In this study, the reductive capacity of whole cell of Leuconostoc mesenteroides N6, Weissella paramesenteroides N7, Weissella cibaria N9, and Leuconostoc pseudomesenteroides N13 was investigated as whole-cell biocatalysts in the enantioselective reduction of (E)-4-phenylbut-3-en-2-one (1).

Regioselective asymmetric bioreduction of trans-4-phenylbut-3-en-2-one by whole-cell of Weissella cibaria N9 biocatalyst.

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10.1021/acs.jafc.1c04873

In this study, we designed a whole-cell biocatalyst to achieve the glycosylation of steviol to Rub in Saccharomyces cerevisiae.

Whole-Cell Biocatalyst for Rubusoside Production in Saccharomyces cerevisiae.

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10.1016/j.ymben.2021.04.005

Robust and efficient enzymes are essential modules for metabolic engineering and synthetic biology strategies across biological systems to engineer whole-cell biocatalysts.

Engineering promiscuity of chloramphenicol acetyltransferase for microbial designer ester biosynthesis.

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10.1002/bit.27938

The obligate aerobic nature of Pseudomonas putida, one of the most prominent whole-cell biocatalysts emerging for industrial bioprocesses, questions its ability to be cultivated in large-scale bioreactors, which exhibit zones of low dissolved oxygen tension.

Pseudomonas putida KT2440 endures temporary oxygen limitations.

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10.1021/acs.jafc.1c03960

coli-based whole-cell biocatalysts for the eco-friendly synthesis of gallic acid from lignin-derived renewable feedstocks.

One-Pot Bioconversion of Lignin-Derived Substrates into Gallic Acid.

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10.1007/s00449-020-02484-5

Due to the unique advantages comparing with traditional free enzymes and chemical catalysis, whole-cell biocatalysts have been widely used to catalyze reactions effectively, simply and environment friendly.

Cell-surface engineering of yeasts for whole-cell biocatalysts

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10.1016/j.tibtech.2020.12.009

Recently, several studies have proven the potential of cyanobacteria as whole-cell biocatalysts for biotransformation.

Biocatalysis in Green and Blue: Cyanobacteria.

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10.1039/D1GC01120K

The preparative biotransformation using a whole-cell biocatalyst in the absence of any exogenous cofactor displayed a space–time yield of 768 g L−1 d−1 and a total turnover number (TTN) of 20 363 for NAD+ recycling.

Design of a self-sufficient hydride-shuttling cascade for concurrent bioproduction of 7,12-dioxolithocholate and l-tert-leucine

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10.1021/acs.jafc.0c07697

In this study, we biosynthesized RK via the catalytic reduction of 4-hydroxybenzylidenacetone using a whole-cell biocatalyst.

Efficient Biosynthesis of Raspberry Ketone by Engineered Escherichia coliCoexpressing Zingerone Synthase and Glucose Dehydrogenase.

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10.3390/ijms22147600

This study aimed to examine the utility of whole-cell biocatalysts for obtaining 3-butyl-3-hydroxyphthalide, which is the metabolite formulated during mammalian metabolism of 3-n-butylidenephthalide.

Microbial Synthesis and Evaluation of Fungistatic Activity of 3-Butyl-3-hydroxyphthalide, the Mammalian Metabolite of 3-n-Butylidenephthalide

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10.3389/fctls.2021.683248

Furthermore, the whole-cell biocatalyst was evaluated in a stirred-tank bioreactor setup with a continuous cyclohexane supply via the gas phase.

Conversion of Cyclohexane to 6-Hydroxyhexanoic Acid Using Recombinant Pseudomonas taiwanensis in a Stirred-Tank Bioreactor

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10.1016/j.enzmictec.2021.109811

The whole-cell biocatalyst could be reused over four times at an average of 97 % conversion when supplied half of fresh cells in the reaction.

Whole-cell biocatalyst for cadaverine production using stable, constitutive and high expression of lysine decarboxylase in recombinant Escherichia coli W3110.

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10.1186/s12934-020-01506-x

coli pET-21b-MBP- laad /pET-28a- dapdh - fdh whole-cell biocatalyst asymmetrically catalyzed the stereoinversion of 150 mM l -Phe to d -Phe, with quantitative yields of over 99% ee in 24 h, by the addition of 15 mM NADP + and 300 mM ammonium formate.

Highly selective synthesis of d-amino acids via stereoinversion of corresponding counterpart by an in vivo cascade cell factory

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10.1002/biot.201800615

This study shows that molybdenum hydroxylase-based whole-cell biocatalysts enable completely anaerobic carbon oxyfunctionalization, when coupled to alternative respiration schemes such as nitrate respiration.

Anaerobic C-H oxyfunctionalization - coupling of nitrate reduction and quinoline hydroxylation in recombinant Pseudomonas putida.

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10.1007/s00253-019-09725-y

The strain was immobilised in calcium alginate beads and repeatedly used as a whole-cell biocatalyst.

Haloferax volcanii as immobilised whole cell biocatalyst: new applications for halophilic systems

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10.1007/s00253-019-09667-5

The development of arming yeast strains as whole-cell biocatalysts involves a selection of effective anchoring proteins to display enzymes and proteins on yeast cell surface.

Identification and evaluation of novel anchoring proteins for cell surface display on Saccharomyces cerevisiae

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10.1002/biot.201800724

Oxygenase-containing cyanobacteria constitute promising whole-cell biocatalysts for oxyfunctionalization reactions.

Light-Dependent and Aeration-Independent Gram-Scale Hydroxylation of Cyclohexane to Cyclohexanol by CYP450 Harboring Synechocystis sp. PCC 6803.

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10.3390/molecules24142550

coli whole-cell biocatalysts were used in batch operated stirred-tank reactors on a 0.

Asymmetric Whole-Cell Bio-Reductions of (R)-Carvone Using Optimized Ene Reductases

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10.1016/j.jbiotec.2019.09.005

Ethanol and longer-chain alcohols are supposed to play a key role since they are relatively easy to produce, using microorganisms as whole-cell biocatalysts.

The emerging role of aldehyde:ferredoxin oxidoreductases in microbially-catalyzed alcohol production.

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10.1021/acssynbio.8b00519

Because these problems are associated with the mechanism of fatty acid transport through membranes, a whole-cell biocatalyst that can form caveolae-like structures was generated to promote substrate endocytosis.

Endocytosing Escherichia coli as a Whole-Cell Biocatalyst of Fatty Acids.

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10.3390/CATAL9020164

Moreover, a multilayer biofilm with cytosolic-expressed LbADH served as a whole-cell biocatalyst.

Self-Immobilizing Biocatalysts Maximize Space–Time Yields in Flow Reactors

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10.1016/j.jbiotec.2019.05.017

In the current work we present the application of a novel short chain dehydrogenase from Bacillus megaterium in an in vivo whole-cell biocatalyst system for the conversion of the intermediate nootkatol into the industrially valuable (+)-nootkatone.

A novel short chain dehydrogenase from Bacillus megaterium for the conversion of the sesquiterpene nootkatol to (+)-nootkatone.

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10.1039/c9ra06301c

In addition, the cascade reaction system was also used to stereoinvert a variety of aromatic and aliphatic L-amino acids to the corresponding D-amino acids by combining the PmLAAD whole-cell biocatalyst with the StDAPDH variant.

Highly selective synthesis of d -amino acids from readily available l -amino acids by a one-pot biocatalytic stereoinversion cascade

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10.1038/s41929-019-0321-8

In this study, we have developed a quantitative approach to whole-cell biocatalyst characterization, enabling a comprehensive study of how yeast-surface displayed multi-enzyme assemblies form.

Elucidating structure-performance relationships in whole-cell cooperative enzyme catalysis

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10.1007/s12010-019-03154-3

Finally, an efficient process of recycling and reusing of SO 4 2− /SnO 2 -bentonite catalyst and immobilized whole-cell biocatalyst was developed for the chemoenzymatic synthesis of furfuryl alcohol from biomass in the one-pot reaction system.

Chemoenzymatic Synthesis of Furfuryl Alcohol from Biomass in Tandem Reaction System

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10.1186/s13068-019-1374-2

Consolidated bio-saccharification (CBS) is considered a promising process for lignocellulose solubilization depending on whole-cell biocatalysts that simultaneously perform effective cellulase production and hydrolysis.

Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization

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10.3390/d11110204

In recent years, the use of whole-cell biocatalysts and biocatalytic enzymes in biotechnological applications originating from the genus Pseudomonas has greatly increased.

Comparative genomic analysis of the biotechnological potential of the novel species Pseudomonas wadenswilerensis CCOS 864T and Pseudomonas reidholzensis CCOS 865T

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10.1007/s11696-019-00758-1

Gluconobacter oxydans can be used as a whole-cell biocatalyst in many industrial processes focused on production of carbonyl and carboxylic compounds by oxidation of respective alcohols.

2-Phenylethanol biooxidation by Gluconobacter oxydans: influence of cultivation conditions on biomass production and biocatalytic activity of cells

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10.3389/fbioe.2019.00444

Recombinant whole-cell biocatalysts are widely used for biotransformation of valuable products.

Plasmid Display for Stabilization of Enzymes Inside the Cell to Improve Whole-Cell Biotransformation Efficiency

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10.1021/ACS.IECR.8B04617

Recently, the nonionic surfactant ROKAnol NL5 was identified as a suitable surfactant for this purpose, since it forms an upper micellar phase, enabling an easy separation of whole-cell biocatalysts.

Long-Chain Alcohol-Modified Micellar Systems and Their Application in a Continuous Extraction Process

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10.1021/acs.nanolett.9b00066

Baker's yeast was used as an exemplary live whole-cell biocatalyst, and the printed structures displayed high resolution, large scale, high catalytic activity and long-term viability.

Direct Writing of Tunable Living Inks for Bioprocess Intensification.

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10.1186/s12934-019-1124-y

In this present study, we aimed at constitutive expression of a mannanase from Bacillus licheniformis DSM13, which was subsequently displayed on the cell surface of Lactobacillus plantarum WCFS1, for use as whole-cell biocatalyst in oligosaccharide production.

Constitutive expression and cell-surface display of a bacterial β-mannanase in Lactobacillus plantarum

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10.1016/J.PROCBIO.2019.01.005

Cell surface display involves the anchoring of enzymes on the surface of cells to be used as whole-cell biocatalysts.

Expression of xylanase on Escherichia coli using a truncated ice nucleation protein of Erwinia ananas (InaA)

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10.3390/ijms20194787

The application of purified enzymes as well as whole-cell biocatalysts in synthetic organic chemistry is becoming more and more popular, and both academia and industry are keen on finding and developing novel enzymes capable of performing otherwise impossible or challenging reactions.

Rhodococcus as a Versatile Biocatalyst in Organic Synthesis

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10.1007/s10295-019-02184-4

The selection of a small solubility-enhancing protein tag and exploitation of the T7 polymerase-induction system allowed the formation of increased levels of functional recombinant protein, thereby improving the performance of the whole-cell biocatalyst.

Improving an Escherichia coli-based biocatalyst for terpenol glycosylation by variation of the expression system

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10.1039/c9gc02499a

Finally, efficient recycling and reuse of the Sn-sepiolite catalyst and immobilized TS whole-cell biocatalyst were developed for the synthesis of furoic acid from rice straw in the one-pot reaction system.

An effective hybrid strategy for converting rice straw to furoic acid by tandem catalysis via Sn-sepiolite combined with recombinant E. coli whole cells harboring horse liver alcohol dehydrogenase

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10.1186/s13036-019-0154-7

coli strains harboring StGAD, SsGAD and ScGAD were used as whole-cell biocatalysts for production of GABA from L-Glu.

Identification of new glutamate decarboxylases from Streptomyces for efficient production of γ-aminobutyric acid in engineered Escherichia coli

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10.1038/s41929-019-0412-6

Specifically, the combination of the whole-cell biocatalysts with the chemical metathesis catalyst allowed the production of cyclopentene, cyclohexene and cycloheptene in one pot from bio-based resources.

Cycloalkenes from plant oil in one pot

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What is/are Whole Cell Biocatalyst?

Whole Cell Biocatalyst - Furthermore, the production of l-threo-β-hydroxy-His and l-threo-β-hydroxy-Gln was achieved using Escherichia coli expressing the gene encoding AEP14369 as a whole-cell biocatalyst. [1] YGSMI21 can be regarded as a whole-cell biocatalyst in the production of various (S)-CSO with the chlorine group at a different position. [2] After optimizing the gene dose ratio of mbSUS and UGT76G1, which is an effective whole-cell biocatalyst GS115 (U76/mb3:1) was constructed. [3] Escherichia coli-based whole-cell biocatalysts are widely used for the sustainable production of value-added chemicals. [4] A whole-cell biocatalyst expressing the engineered reductase and a yeast formate dehydrogenase for NADH-recycling provided substantial stabilization of the reductase. [5] The yield of levan produced by these two whole-cell biocatalysts reaches 26 g/L and 34 g/L in 24 h, respectively. [6] Furthermore, a summary of recent developments pertaining to the potential applications of decoy molecules from the development of a whole-cell biocatalyst to their use in crystallography will be discussed. [7] coli strain that expressed GADZ11 was used as a whole-cell biocatalyst for the production of GABA. [8] In this study, the reductive capacity of Lactobacillus paracasei BD101 was investigated as whole-cell biocatalyst in the enantioselective reduction of 1-tetralone (1). [9] In this study, a novel whole-cell biocatalyst that can accelerate the degradation of antibiotics was developed by displaying β-lactamase on the cell surface of Aspergillus niger. [10] The engineered whole-cell biocatalyst was used in four sequential batches to achieve a production of 126 mM ε-caprolactone with a high molar yield of 0. [11] In our previous study, one-step pyruvate and D-alanine production from D,L-alanine by a whole-cell biocatalyst Escherichia coli expressing L-amino acid deaminase (Pm1) derived from Proteus mirabilis was investigated. [12] In-Cell Crosslinked Enzymes (InCLEs) is a procedure to improve the thermal and operational stability of Bacillus megaterium intracellular decarboxylase activity, allowing to obtain enhanced whole-cell biocatalyst to produce 4-vinyl guaiacol (4VG). [13] Background The display of recombinant proteins on cell surfaces has a plethora of applications including vaccine development, screening of peptide libraries, whole-cell biocatalysts and biosensor development for diagnostic, industrial or environmental purposes. [14] With respect to biotechnological application, Escherichia coli whole-cell biocatalysts were developed and characterized in small-scale biotransformations. [15] coli strain was used as a whole-cell biocatalyst to produce trans-cinnamic acid from l-Phe. [16] In this study, we aimed to develop a Bacillus megaterium based whole-cell biocatalyst for the bio-production of 3-aminopropionic acid (3-APA). [17] A whole-cell biocatalyst based on the engineered reductase and a yeast formate dehydrogenase for NADH-recycling provided substantial stabilization of the reductase. [18] This chapter deals with the construction and application of a whole-cell biocatalyst by displaying IsPETase on the surface of yeast (Pichia pastoris) cells. [19] In this study, low-cost sugarcane molasses was used to produce whole-cell biocatalysts containing transfructosylating enzymes by Aureobasidium pullulans FRR 5284, followed by FOS production from molasses using the whole-cells of A. [20] Quercetin (1) was converted into quercetin 7-O-succinyl glucoside (2) by used Bacillus amyloliquefaciens FJ18 as a solvent-resistant whole-cell biocatalyst. [21] We report an OmpF loop deletion mutant, which improves the cellular uptake of external additives into an Escherichia coli whole-cell biocatalyst. [22] coli into a whole-cell biocatalyst for hydrogen-driven CO2 reduction to formate without the need of any external co-factors or endogenous enzymes in the reaction process. [23] Concerning the latter, the two whole-cell biocatalysts were coupled with laccase/TEMPO in a one-pot reaction designed to enable catalysis of the three oxidative steps necessary to convert HMF into 2,5-furandicarboxylic acid (FDCA), a compound with immense potential in the production of sustainable and eco-friendly polymers. [24] In this work, a yeast-like fungus, Aureobasidium subglaciale F134 was discovered as a whole-cell biocatalyst for BHMF synthesis from selective reduction of HMF. [25] coli strain that expressed GADZ11 was used as a whole-cell biocatalyst for the production of GABA. [26] In this study, the reductive capacity of whole cell of Leuconostoc mesenteroides N6, Weissella paramesenteroides N7, Weissella cibaria N9, and Leuconostoc pseudomesenteroides N13 was investigated as whole-cell biocatalysts in the enantioselective reduction of (E)-4-phenylbut-3-en-2-one (1). [27] In this study, we designed a whole-cell biocatalyst to achieve the glycosylation of steviol to Rub in Saccharomyces cerevisiae. [28] Robust and efficient enzymes are essential modules for metabolic engineering and synthetic biology strategies across biological systems to engineer whole-cell biocatalysts. [29] The obligate aerobic nature of Pseudomonas putida, one of the most prominent whole-cell biocatalysts emerging for industrial bioprocesses, questions its ability to be cultivated in large-scale bioreactors, which exhibit zones of low dissolved oxygen tension. [30] coli-based whole-cell biocatalysts for the eco-friendly synthesis of gallic acid from lignin-derived renewable feedstocks. [31] Due to the unique advantages comparing with traditional free enzymes and chemical catalysis, whole-cell biocatalysts have been widely used to catalyze reactions effectively, simply and environment friendly. [32] Recently, several studies have proven the potential of cyanobacteria as whole-cell biocatalysts for biotransformation. [33] The preparative biotransformation using a whole-cell biocatalyst in the absence of any exogenous cofactor displayed a space–time yield of 768 g L−1 d−1 and a total turnover number (TTN) of 20 363 for NAD+ recycling. [34] In this study, we biosynthesized RK via the catalytic reduction of 4-hydroxybenzylidenacetone using a whole-cell biocatalyst. [35] This study aimed to examine the utility of whole-cell biocatalysts for obtaining 3-butyl-3-hydroxyphthalide, which is the metabolite formulated during mammalian metabolism of 3-n-butylidenephthalide. [36] Furthermore, the whole-cell biocatalyst was evaluated in a stirred-tank bioreactor setup with a continuous cyclohexane supply via the gas phase. [37] The whole-cell biocatalyst could be reused over four times at an average of 97 % conversion when supplied half of fresh cells in the reaction. [38] coli pET-21b-MBP- laad /pET-28a- dapdh - fdh whole-cell biocatalyst asymmetrically catalyzed the stereoinversion of 150 mM l -Phe to d -Phe, with quantitative yields of over 99% ee in 24 h, by the addition of 15 mM NADP + and 300 mM ammonium formate. [39] This study shows that molybdenum hydroxylase-based whole-cell biocatalysts enable completely anaerobic carbon oxyfunctionalization, when coupled to alternative respiration schemes such as nitrate respiration. [40] The strain was immobilised in calcium alginate beads and repeatedly used as a whole-cell biocatalyst. [41] The development of arming yeast strains as whole-cell biocatalysts involves a selection of effective anchoring proteins to display enzymes and proteins on yeast cell surface. [42] Oxygenase-containing cyanobacteria constitute promising whole-cell biocatalysts for oxyfunctionalization reactions. [43] coli whole-cell biocatalysts were used in batch operated stirred-tank reactors on a 0. [44] Ethanol and longer-chain alcohols are supposed to play a key role since they are relatively easy to produce, using microorganisms as whole-cell biocatalysts. [45] Because these problems are associated with the mechanism of fatty acid transport through membranes, a whole-cell biocatalyst that can form caveolae-like structures was generated to promote substrate endocytosis. [46] Moreover, a multilayer biofilm with cytosolic-expressed LbADH served as a whole-cell biocatalyst. [47] In the current work we present the application of a novel short chain dehydrogenase from Bacillus megaterium in an in vivo whole-cell biocatalyst system for the conversion of the intermediate nootkatol into the industrially valuable (+)-nootkatone. [48] In addition, the cascade reaction system was also used to stereoinvert a variety of aromatic and aliphatic L-amino acids to the corresponding D-amino acids by combining the PmLAAD whole-cell biocatalyst with the StDAPDH variant. [49] In this study, we have developed a quantitative approach to whole-cell biocatalyst characterization, enabling a comprehensive study of how yeast-surface displayed multi-enzyme assemblies form. [50]

one pot reaction

Concerning the latter, the two whole-cell biocatalysts were coupled with laccase/TEMPO in a one-pot reaction designed to enable catalysis of the three oxidative steps necessary to convert HMF into 2,5-furandicarboxylic acid (FDCA), a compound with immense potential in the production of sustainable and eco-friendly polymers. [1] Finally, an efficient process of recycling and reusing of SO 4 2− /SnO 2 -bentonite catalyst and immobilized whole-cell biocatalyst was developed for the chemoenzymatic synthesis of furfuryl alcohol from biomass in the one-pot reaction system. [2] Finally, efficient recycling and reuse of the Sn-sepiolite catalyst and immobilized TS whole-cell biocatalyst were developed for the synthesis of furoic acid from rice straw in the one-pot reaction system. [3]