Introduction to Whole Bone

Disruption of the gut microbiome in this manner has been shown to lead to reductions in tissue mechanical properties and whole bone strength in adulthood with only minor changes in bone geometry and density.

RESULTS There were no differences in in vitro bone cell recruitment from whole bone marrow or in bone turnover markers CTX-I or osteocalcin.

Based on the evidence from clinical trials, transplantation of cells, most commonly whole bone marrow aspirates (BMA) or bone marrow aspirate concentrations (BMAC), in combination with biomaterial scaffolds has demonstrated an additional effect in sinus augmentation and horizontal ridge augmentation, and comparable bone regeneration to autogenous bone in alveolar cleft repair.

METHODS Whole bone marrow mononuclear cells (wBMMNC, 5×106) and enriched c-Kit+ hematopoietic stem/progenitor cells (HSPC, 3×105) from the BM of B6-Ly5.

Both clinical and animal studies suggest that pregnancy and lactation lead to adaptations in cortical bone structure to allow for rapid calcium release from the trabecular compartment while maintaining whole bone stiffness and strength.

The aim of this cross-sectional study was to investigate the effects of ADT in men with PCa on BMD, bone structure, estimates of whole bone strength and cortical bone distribution.

02) and MRI measures of trabecular number, trabecular thickness, and whole bone stiffness were lower (all p < 0.

Based on limited and heterogeneous clinical evidence, implantation of cells (mostly whole bone marrow), in combination with biomaterial scaffolds results in bone regeneration which is (a) superior compared to implantation of scaffolds alone in sinus and horizontal ridge augmentation, and (b) comparable to autogenous bone in alveolar cleft repair.

Methods The BMSCs were isolated by the method of whole bone marrow adherent, and sub-cultured.

However, marrow cells are also subjected to mechanical stress during whole bone loading, and may contribute to mechanically regulated bone physiology.

Accurately estimating whole bone strength is critical for identifying individuals that may benefit from prophylactic treatments aimed at reducing fracture risk.

BMSCs were isolated and cultured with whole bone marrow adherence method.

Methods BMMSCs were isolated by whole bone marrow adherence and identified by detecting Cluster of Differentiation 29 (CD29), Cluster of Differentiation 90 (CD90), Classical class Ⅰ major histocompatibility complex (RT1-A), Cluster of Differentiation 34 (CD34), Cluster of Differentiation 45 (CD45) and Classical class Ⅱ major histocompatibility complex (RT1-B) by Flow cytometry.

The bone defects were treated with whole bone marrow loaded on gel foam in group A right tibias and using mononuclear cell layer obtained through centrifuging bone marrow loaded on gel foam in group B right tibias.

There was no relationship between BMSi values with any measurement of mineral content of whole bone tissue (BMD, BMDD) or maturity/crystallinity of bone mineral.

MethodsBM-MSCs were collected from three de novo acute lymphoblastic leukemia (ALL) and three aplastic anemia patients (AA), which were cultivated by the whole bone marrow adherent method.

Transplantation of Eng-deleted whole bone marrow or purified HSCs into lethally irradiated mice results in a profound engraftment defect in tertiary and quaternary recipients.

However, no open-source, time-efficient methods are available to acquire these parameters for whole bones.

1 B6 recipients were lethally irradiated and transplanted with whole bone marrow cells from either wild type CD45.

As the growing skeleton contains non-ossified cartilaginous regions at the epiphyses, which are not well characterised on CT examinations, it is difficult to evaluate the mechanical behaviour of the developing whole bone.

Higher skeletal fragility has been established for the Brtl/+ mouse model of osteogenesis imperfecta at the whole bone level, but previous investigations of mechanical properties at the bone material level were inconclusive.

This study examines the clinical effect of whole bone marrow (WBM) and bone marrow concentrate (BMC) injections in patients who were diagnosed with rotator cuff tears or shoulder osteoarthritis.

Whole bone pairing and each bone portion was analyzed using the Part Comparison tool in 3‐matic.

RATIONALE Whole bone proteomic analyses rely on lengthy sample preparation including demineralization and digestion to break bone down into peptides to recover using mass spectrometry.

Here, we explore near-infrared spectroscopy as a tool for gauging the collagen content of ground and whole bone from about 500 to 45,000 years ago.

Based on HRpQCT images, we have developed a plate rod µFE (PRµFE) method for whole bone segments using individual trabecula segmentation (ITS) and an adaptive cortical meshing technique.

The fracture mechanism at this location is necessary for understanding the comprehensive fracture of whole bone.

In this review we summarise over 15 years of research and development around the prediction of whole bones strength from Computed Tomography data, with particular reference to the prediction of the risk of hip fracture in osteoporotic patients.

In whole bone bending tests, measures of bone bending strength were similar across groups; however, Longshanks tibiae were significantly more rigid, more brittle, and required less than half the energy to fracture.

Microdamage, lacunar-canaliculi architecture, vascular porosity, lamellar organization, and whole bone cross-sectional shape are individually considered.

Vertebral whole bone strength is substantially affected by cortical bone properties.

For the initial diagnosis a conventional radiograph of the whole bone in two planes is sufficient.

Through the combined application of a number of diagenetic parameters (general histological index; infrared splitting factor; carbonate/phosphate ratio; amide/phosphate ratio; col wt%; % C, % N and C/N of whole bone and collagen; % endogenous DNA), we provide new insights into petrous bone micromorphological characteristics and diagenesis, and new evidence to enhance screening practices for aDNA and collagen analysis.

After scanning, humeri, femurs, and tibias were cleaned, divided into epiphysis (E) and diaphysis (D), fat extracted, ashed, and digested under nitric acid, and Ca was determined by atomic absorption spectroscopy was used to determine E, D, and whole bone Ca.

36 SD greater whole bone stiffness and 0.

Macroscopic skeletal analyses provide insight into species origin when whole bones are recovered but are frequently rendered inapplicable when bones are fragmented.

Thus, a specific activating mutation in MEK1, expressed only by osteoprogenitors postnatally, can have a significant impact on bone strength through complex alterations in whole bone geometry, bone micro-structure, and bone matrix.

Background We previously reported regenerative therapies for decompensated cirrhosis based on peripheral venous drip infusion using non-cultured whole bone marrow (BM) cells, or the less invasive cultured BM-derived mesenchymal stem cells (BMSCs).

Amplicon-based next-generation sequencing (NGS) targeting entire coding or hotspot regions of a 38-multigene panel associated with myeloid malignancies was performed on whole bone marrow DNA using a Ion Torrent platform (Thermo Fisher Scientific, Inc.

However, evaluation of trabecular bone structure and whole bone shape is often qualitative or semiquantitative.

0 ppm from whole bone, making it amenable to rigorous quantitative analysis.

We have found that in mice, conditional disruption of VEGF in osteocalcin‐expressing cells (OcnVEGFKO) exerts a divergent influence on morphological, cellular, and whole bone properties between sexes.

Whole bone marrow samples were incubated for 48 h in well plates containing IDA, CYT, or their combination.

Dietary treatment and growth classification were the fixed effects to evaluate gait, OC, tibia bending measures, metacarpal mineralization; and using computerized tomography, the carcass composition, bone size, and whole bone density (WBD).

Wethers were harvested on d 41 and a whole bone-in loin and a boneless inside leg roast were fabricated from one side each of carcass.

The validated relationships have also been adapted for use in whole bone scapular models and have the potential to dramatically increase the accuracy of clinical-resolution CT-derived shoulder finite element studies.

Serum tartrate-resistant acid phosphatase 5b and urine N-terminal cross-linked telopeptide of type I collagen were both elevated, and bone scintigraphy revealed increase of whole bone uptake.

We performed a wide phenotyping of immune cells by flow cytometry in fresh whole blood, and fresh whole bone marrow at diagnosis of children diagnosed for neuroblastoma.

It was determined that incorporating four cylindrical HAp columns, fabricated under 44 MPa of pressure and sintered at 1200°C for 5 hr, led to load-bearing properties that match the yield strength of native whole bone.

In this study, various sample mass (1 × 50 mg, 3 × 50 mg, and 1 × 150 mg chip(s)) and incubation times (2, 4, and 16 h) were tested using the PrepFiler® BTA™ Forensic DNA Extraction Kit to digest whole bone chips in lieu of powdering.

Methods Bone marrow mesenchymal stem cells (BMSCs) of Sprague Dawley rats were isolated and cultured using a whole bone marrow adherent method.

The mean degree of mineralization (DMB) and heterogeneity index (HI) of mineralization were assessed by X-ray microradiography on whole bone matrix; intrinsic properties (mineral/organic ratio, mineral maturity, mineral crystallinity, collagen maturity) were assessed by Fourier Transform Infrared microspectroscopy, microhardness by microindentation, both at a global level and calculated by mean of several measurements over the whole tissue area.

1 mice received non-myeloablative busulfan conditioning (25 mg/kg), full intensity head-shielded irradiation, full intensity busulfan conditioning (125 mg/kg) prior to transplant with whole bone marrow from CD45.

By HR-pQCT, 1/3RO had abnormalities at both radius and tibia compared to controls: lower total, cortical and trabecular vBMD, cortical thickness and trabecular number, higher trabecular separation and heterogeneity, and lower whole bone stiffness.

MethodsBMSCs were isolated from the 4-week-old male Sprague Dawley rats by whole bone marrow adherent culturing, and the characteristics were verified by morphology, immunophenotype, adipogenic, and osteogenic differentiation potential.

Conditioned mice were transplanted with 2 × 107 whole bone marrow cells, and chimerism assessed over 12 weeks.

The ability to map cellular and molecular changes to nano-scale chemical composition changes (mineral, collagen cross-links) and structural changes (porosity, lacuno-canalicular network) to whole bone mechanics is at the forefront of an exciting era of discovery.

Disruption of the gut microbiome in this manner has been shown to lead to reductions in tissue mechanical properties and whole bone strength in adulthood with only minor changes in bone geometry and density.

Prior work characterized the response of individual mouse strains but did not establish whether the impact of Scl-Ab on whole bone strength would vary across different inbred mouse strains.

The hierarchical pattern of KIT, SF3B1, JAK2, and additional mutations was studied in whole and fractionated subpopulations of peripheral blood cells and whole bone marrow.

With homogenous material properties applied to the proximal tibia mesh using FEA, there was (1) an increase in whole bone (trabecular + cortical) structural stiffness from IRR compared to NR mice within Sham (+ 10%) and OVX (+ 15%); (2) a decrease in stiffness from OVX versus Sham within NR mice (− 18%) and IRR mice (− 14%).