Introduction to A2b Receptor Knockdown

What is/are Receptor Knockdown?

Receptor Knockdown - Through receptor knockdown and inhibitor experiments, we demonstrated that COL11A1 significantly upregulates HSP27 phosphorylation and expression via DDR2/integrin α1β1 and Src/Akt signaling in ovarian cancer cells. ^[1] Furthermore, these findings advise caution in the interpretation of functional consequences after 7TM receptor knockdown in experimental models. ^[2] Consequently, we observed the effect of damaged astrocytes on neurite outgrowth and regeneration, and the results showed that damaged astrocytes hindered neurite outgrowth and regeneration; however, the inhibitory effect of injured astrocytes on neurite regeneration was reduced following ephrin-B receptor knockdown or inflammatory inhibition at 24 h after astrocyte injury. ^[3] Consistent with a role for mGlu3 in PFC pyramidal cells, mGlu3-dependent amygdala-cortical LTD was eliminated following mGlu3 receptor knockdown. ^[4] Based on endocytosis inhibitors and receptor knockdown, the cellular uptake of P2Ns-GA is clathrin-mediated and dependent on TfR1 expression, but other trafficking mechanisms, particularly those involving caveo-lae/lipid rafts, can also play a role. ^[5] We reported that chow-fed mice, with adult-onset, hepatocyte-specific GH receptor knockdown (aHepGHRkd) develop steatosis, and with age, a mild NASH-like phenotype. ^[6] The primary effect of BNST-mGlu5 receptor knockdown was that it independently enhanced anhedonia-like activity in females. ^[7] Hepatic Klf15 expression is significantly increased by the attenuation of the insulin signaling in STZ-induced insulin deficiency and insulin receptor knockdown models. ^[8] Insulin receptor knockdown inhibited proliferation and induced programmed cell death. ^[9] ETH receptor knockdown either in the mushroom body (MB) γ lobe or in octopaminergic dorsal-anterior-lateral (DAL) neurons impairs memory performance, indicating its direct action in these brain areas. ^[10] NP‐mediated TGFBR2 receptor knockdown protected NK cells against TGF‐β suppression, which was studied in both two‐dimensional and three‐dimensional lung cancer cell culture systems. ^[11] Interestingly, 5-HT1A receptor knockdown did not significantly alter anxiety-like behavior in male or female mice, which is in agreement with previous findings that anxiety and fear are modulated by dissociable circuits in the BNST. ^[12] With the debate on the consequences of this particular receptor knockdown still going on and the after-shocks in the form of queries expected to continue for some time in the future, we enter the arena of this particular genome editing as first responders with concerns regarding the neurological aftermath of CCR5 knockout in the babies born. ^[13] Consistently, chemerin receptor knockdown reduced EC and VSMC dysfunction. ^[14] To understand the biological function of melatonin receptors in hCG-induced testosterone synthesis, we generated melatonin receptor knockdown cells using specific siRNA and performed testosterone detection after hCG treatment. ^[15] A combination of altered social conditions, pharmacological manipulation and 5-HT2 receptor knockdown by siRNA revealed an inhibitory role of this receptor subtype on aggression. ^[16] To understand these differences, we tested impact of type I receptor knockdown and ERFE on hepcidin induction by the heterodimeric BMP2/6 ligand. ^[17] A2B receptor knockdown prevented the effects of 2-chloroadenosine on cyclic AMP production and P-SMC proliferation and migration. ^[18] EP receptor knockdown and subtype‐selective antagonists demonstrated EP2 and EP4 receptor responsiveness in HASM cells to the specific ONO compounds, whereas PGE2 appeared to preferentially signal via the EP4 receptor. ^[19] Lastly, we showed that the GPR171 antagonist or receptor knockdown decreases signaling by the mu-opioid receptor, but not the delta-opioid receptor. ^[20] A2B receptor antagonist treatment or A2B receptor knockdown suppressed EGFR translocation, γH2AX/53BP1 focus formation, and colony formation of lung cancer cell lines A549, calu-6 and NCI-H446, compared with a normal cell line (beas-2b). ^[21]

A2b Receptor Knockdown

A2B receptor knockdown prevented the effects of 2-chloroadenosine on cyclic AMP production and P-SMC proliferation and migration. ^[1] A2B receptor antagonist treatment or A2B receptor knockdown suppressed EGFR translocation, γH2AX/53BP1 focus formation, and colony formation of lung cancer cell lines A549, calu-6 and NCI-H446, compared with a normal cell line (beas-2b). ^[2]

Insulin Receptor Knockdown

Hepatic Klf15 expression is significantly increased by the attenuation of the insulin signaling in STZ-induced insulin deficiency and insulin receptor knockdown models. ^[1] Insulin receptor knockdown inhibited proliferation and induced programmed cell death. ^[2]