Introduction to Quantitative Single

Closely watching those quantitative single-cell optical technologies, we are on the track of obtaining better resolution and higher throughput while measuring single cells (Table 1).

Using high-throughput reverse genetic screens based on quantitative single-cell fluorescence measurements, we identified the Hda1 histone deacetylase complex (Hda1C) as a repressor of DNC transcription.

Here, we report results and demonstrate the applicability of quantitative single-cell spatial proteomics analyses in the TiME of primary and recurrent human papillomavirus (HPV)-negative head and neck squamous cell carcinoma (HNSCC) tumors.

High levels of GFP loading into secreted EVs was confirmed by Western blotting for specific EV‐sorting domains, but quantitative single‐vesicle analysis by Nanoflow cytometry detected GFP in less than half of the particles analysed, reflecting EV heterogeneity.

Quantitative single cell analysis showed that invasion can result in host membrane breaching at different stages of invasion and cell death, or in traversal of host cells without membrane breaching.

By creating a multitask deep learning framework referred to as DeepLIIF, we present a single-step solution to stain deconvolution/separation, cell segmentation, and quantitative single-cell IHC scoring.

These require an assessment of: a) the total number of cells, b) the total number of cell types, and c) the complete and quantitative single molecular detection and identification for all classes of biopolymers, and organic and inorganic compounds, in each individual cell.

The approach paves the way for routine, precise, quantitative single-cell proteomics.

By using small-sized carbon dots (CDs), we have now developed a quantitative single-molecule localization microscopy technique (qSMLM) based on spontaneous blinking to count single molecules with a localization precision of 10 nm, which can be accomplished on conventional microscopes without sophisticated laser control.

Methods: A quantitative single-group correlational analysis was used to survey certified NM technologists and students in the Nuclear Medicine Technology Certification Board e-mail database (n = 20,389).

This study aimed to explore brain neurometabolic alterations in patients with subjective cognitive decline plus using quantitative single-voxel and multi-voxel 1H-magnetic resonance spectroscopy.

Here, we redevelop our quantitative single-molecule analysis routines into an optimized and extensible Python program, with GUI and command-line implementations to facilitate use on local machines and remote clusters, by beginners and advanced users alike.

The chapter presents a review of the literature on Native American education and the use of culturally responsive instruction to guide the implementation of a computer-based reading program to increase engagement and reading levels for Native American secondary students for a quantitative single-case study on the effects of using Achieve3000 while using a culturally responsive model for selecting reading samples.

These chemical distribution maps can be used as qualitative multi-element maps, as semiquantitative single-element heat maps, and as a basis for a novel image analysis workflow quantifying the modal abundance, size, shape, and degree of sorting of segmented components.

Altogether, our results reveal that quantitative single-cell live imaging has the potential to uncover microbial behaviors that cannot be predicted from batch culture studies, and thereby contribute to our understanding of interactions between pathogens that co-colonize host-associated surfaces during polymicrobial infections.

Regarding their metrics, the majority of NSOs were measured in their occurrence using quantitative single measures, and a few validated tools were used to a lesser extent.

Across multiple contexts we observed highly 'wrinkled' nuclear envelopes, and subsequently developed a quantitative single-cell imaging metric to better evaluate how wrinkles in the nuclear envelope relate to progenitor cell mechanotransduction.

Altogether, our results reveal that quantitative single-cell live imaging has the potential to uncover microbial behaviors that cannot be predicted from batch culture studies, and thereby contribute to our understanding of interactions between pathogens that co-colonize host-associated surfaces during polymicrobial infections.

Quantitative singleplex PCR was performed to evaluate TS.

The mechanical properties were measured by performing nanohardness tests, allowing the extraction of hardness and elastic modulus out of the load/unload behavior of the samples, and the scratch test according to ASTM C1624-05 (2010), Standard Test Method for Adhesion Strength and Mechanical Failure Modes of Ceramic Coatings by Quantitative Singles Point Scratch Testing (Superseded), coupled with acoustic emission analysis, probed adhesion of the TiN and TiAlN coating on the steel substrates.

In this study, we develop a quantitative single-cell surface-dispersal assay and use it to show that P.

Our approach couples a tethered function assay with quantitative single-cell fluorescence measurements to analyze ∼50,000 protein fragments and determine their effects on a tethered mRNA.

Here, we study this scaling phenomenon in human cells by combining genome-wide perturbations with quantitative single-cell measurements.

We highlight the improved efficiency via quantitative single-molecule RNA imaging of mammalian cells with a 2-fold improvement in the fluorescence intensity compared to a conventional SLM-based microscopy.

Here, we use a quantitative single molecule super-resolution imaging technique named qPAINT to quantify complex formation within an example GPCR.

This diversity is largely unexplored at the level of single-cell proteomes because of the limitations of quantitative single-cell protein analysis.

Methods A quantitative single-site study with a convenience sample of 80 APN Portfolios.

You will find more of the most recent advances in the June 2021 Special Issue: Quantitative single-cell optical technologies [3].

Here, we consider examples of both small- and large-scale studies carried out with isogenic cell populations assessed by fluorescence microscopy, and we illustrate the advantages, challenges, and the promise of quantitative single-cell image analysis.

Quantitative single-vesicle imaging using super-resolution microscopy revealed heterogeneous patterns of tetraspanin expressions, being all MSC EV fractions single, double and triple positive, in variable proportions, for CD63, CD81 and CD9.

Upon measurement, large amounts of quantitative single-cell data are generated, which need to be analyzed appropriately.

We identified the Hda1 histone deacetylase complex (Hda1C) as a repressor of DNC in high-throughput reverse genetic screens based on quantitative single-cell fluorescence measurements.

Here, we present N4-acetylcytidine (ac4C) sequencing (ac4C-seq), a protocol for the quantitative single-nucleotide resolution mapping of cytidine acetylation in RNA.

Quantitative single-cell and single-molecule imaging reveals that the oncogenic TF EWS/FLI1 requires a finely tuned range of LCD-LCD interactions to efficiently activate target genes.

This quantitative single-channel duplexing method is an economical substitute for a conventional rather expensive probe-based qPCR requiring different color probes and hardware capable of processing these fluorescent signals.

Measurement of ATP levels in living cells by quantitative single-cell imaging suggests that ATP levels are not the only determinant of SPB movement.

Using a concept of ambiguity-free orientation determination analysis, we present fast and quantitative single-acquisition Raman-based orientation mapping by simultaneous registration of multiple Raman scattering spectra obtained at different polarizations.

Quantitative single-molecule localization reveals the subcellular distribution of a monoamine transporter protein in the axons of a single, identified serotonergic Dorsal Paired Medial (DPM) neuron.

Inspired after a sabbatical, he approached Dominic Grün, an expert in quantitative single-cell biology and scRNA-seq, to help build the new liver cell atlas using his group’s expertise in the experimental and computational methods needed for the project.

We describe here multiple annealing and dC-tailing-based quantitative single-cell RNA-seq (MATQ-seq) with ~90% capture efficiency.

Here, we develop m6A-Crosslinking-Exonuclease-sequencing (m6ACE-seq) to map transcriptome-wide m6A and m6Am at quantitative single-base-resolution.

Here, we provide an overview of quantitative single cell approaches that evaluate and resolve cell-to-cell heterogeneity and CIN, and discuss considerations when selecting the most appropriate approach to suit both research and clinical contexts.

Altogether, these results reveal the dynamics of how phagocytes influence mAb behavior in vivo, and demonstrate an application of intravital microscopy for quantitative single‐cell measurement of mAb distribution and retention in tumors with heterogeneous target expression.

Herein, quantitative single cell analyses using cytometry technologies are key.

To fill this knowledge gap, we conducted a quantitative single-molecule imaging study of endogenous VEGFR-2 in primary endothelial cells, revealing the dynamic nanoscale organization of VEGFR-2 in its native environment.

However, there is a need for quantitative single-cell mass spectrometry (qSCMS) methods to fully characterize drug efficacy from individual cells within cell populations.

Here authors develop and utilize a quantitative single-molecule assay reporting on the kinetics of extrinsic eukaryotic transcription termination by the Sen1 helicase and a reaction intermediate in which the Pol II transcription bubble appears half-rewound.

We developed a quantitative single-molecule fluorescence in situ hybridization-based method to quantify splicing efficiency at transcription active sites in single cells.

The homogenous illumination profile of FFI will enable quantitative single-molecule analysis based on intensity information.

Here, using a combination of quantitative single-cell structural and chemical imaging techniques (FIB-SEM, nanoSIMS, Synchrotron X-ray fluorescence), we show that the structural organization, physiology, and trophic status of the algal symbionts (the haptophyte Phaeocystis) significantly change within their acantharian hosts compared to their free-living phase in culture.

Technologies such as microscopy, sequential hybridization, and mass spectrometry enable quantitative single‐cell phenotypic and molecular measurements in situ.

The study was a repeated measures quasi-experimental, quantitative single group pre-test, mid-test, and post-test design.

The global comparison indices provide a quantitative single value to quickly check the overall similarity of velocity directions and magnitudes between PIV and LES results of a specific individual plane.

Using Bayesian inference, we calibrate this model using quantitative single molecule fluorescence in situ hybridization data.

Compounds 1 and 3 are obtained under hydrothermal conditions, whereas compound 2 is achieved when compound {Zn(bppCOO)2}n (1) undergoes a direct metal exchange process with Cu(II)-salt in a quantitative single crystal to single crystal transformation process (solid−liquid interface reaction), retaining its structural integrity.

The homogenous illumination profile of FFI will enable quantitative single-molecule analysis based on intensity information.

Our strategy uses selective and quantitative single additions of cyclopropene (CPE) derivatives to precisely place functional moieties at desired locations along a polymer chain during the living ring-opening metathesis polymerization (ROMP) of norbornenes (NBEs).

We present multiSLIDE, an open-source tool for query-driven visualization of quantitative single- or multiomics data.

BackgroundFlow cytometry is a popular technology for quantitative single-cell profiling of cell surface markers.

Here, we present Spatial Genomic Analysis (SGA), a quantitative single-cell transcriptional profiling method that takes advantage of single-molecule imaging of individual transcripts for up to a hundred genes.

Here, we evaluate the blinking kinetics of four photoactivatable fluorescent proteins for quantitative single-molecule microscopy.

By employing a live-cell FRET reporter and quantitative single cell imaging, we characterized the dynamic phenotypes and rate-limiting kinetics of a panel of normal and epithelial cancer cells to killing by primary human NK cells.

Our methodology lays the foundation for automated and quantitative single-cell analysis of proteins by mass-spectrometry.

Most recently, new insights have been generated through the use of quantitative single-cell approaches to tackle the problem of cell cycle heterogeneity, through genetic editing in mammalian systems to probe signalling pathways in exquisite detail, and by employing mathematical models to generate nonintuitive insights into the complex systems underpinning cell cycle control.

This permits sub-cellular transcript localization and the addition of quantitative single-cell derived values of mRNA expression levels to our previous analysis.

We first provide a historical context for quantitative single hazard and multi-hazard assessment.

Although optoPlate-96 experiments are compatible with any plate-based readout, we describe analysis using quantitative single-cell immunofluorescence.

Here, the authors use a quantitative single-cell mass imaging strategy to reveal that cellular noise impacts cellular biomass and triacylglycerol accumulation, as well as protein and fatty-acid recycling under starvation, differently.

Here, we report the statistical and quantitative single-molecule measurement of the adsorption at the solid-liquid interface by using the single-molecule break junction technique.

Background We used an ultrasensitive, quantitative single molecule array (Simoa) immunoassay to test whether concentrations of Clostridioides (formerly Clostridium) difficile toxins A and/or B in the stool of adult inpatients with C.

We analyzed CAR-mediated antigen recognition via quantitative single molecule live-cell imaging and found the sensitivity of CAR-T-cells towards antigen approximately 1000-times reduced when compared to T-cell antigen receptor (TCR)-mediated recognition of nominal peptide/MHC complexes.

Quantitative single-molecule dissection methods reveal that catalytic opening of a DHT motif harbouring a toehold triggers successive branch migration, which autonomously propagates to form copolymers with alternate tile units.

The ability to, at least in principle, maintain high imaging contrast independent of the exposure time or the scattering cross section of the object allows for unique applications in single-particle tracking, label-free imaging of nanoscopic (dis)assembly, and quantitative single-molecule characterization.

Using Bayesian inference, we calibrate this model using quantitative single molecule fluorescence in situ hybridization data.

The quantitative single-molecule measurements reveal unusual lower adsorption strength and higher catalytic activity on the confined metal reaction centres within the nanoporous structure.

Here, we robustly assessed heterogeneous EV populations from cultured cell lines via nanoparticle tracking analysis, proteomics, transcriptomics, transmission electron microscopy, and quantitative single molecule localization microscopy (qSMLM).