Introduction to Intracellular Recordings
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Intracellular Recordings sentence examples within Performed Intracellular Recordings
In the present study, we performed intracellular recordings in the MGB of anesthetized guinea pigs and examined whether and how the TRN modulates the SSA of MGB neurons with inhibitory inputs.
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We performed intracellular recordings from muscle of both genetic and pharmacologic mouse models of Becker disease to identify the mechanism underlying transient weakness.
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Intracellular Recordings sentence examples within Cell Intracellular Recordings
Here we tested the utility of Magneto in the adult somatosensory cortex by performing whole-cell intracellular recordings in vitro and extracellular recordings in freely moving mice.
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Here we tested the utility of Magneto in the adult somatosensory cortex by performing whole-cell intracellular recordings in vitro and extracellular recordings in freely moving mice.
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Intracellular Recordings sentence examples within Vivo Intracellular Recordings
In vivo intracellular recordings showed that both SO and DO neurons had significantly shorter post-spike hyperpolarization latency and excitatory duration in response to CF-FM in comparison to CF stimuli, suggesting that the FM component had an inhibitory effect on the responses to the CF component.
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Here, we found profound differences in the information rate and signaling bandwidth between in-vitro patch-clamp and in-vivo intracellular recordings of Periplaneta americana photoreceptors, and traced them to the properties of quantum bumps and membrane resistance.
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Intracellular Recordings sentence examples within Performing Intracellular Recordings
We then validated it by performing intracellular recordings from optogenetically-tagged amygdalar neurons followed by histological reconstruction, which revealed that it is accurate and precise to within ~100 μm.
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We then validated it by performing intracellular recordings from optogenetically-tagged amygdalar neurons followed by histological reconstruction, which revealed that it is accurate and precise to within ∼100 μm.
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Intracellular Recordings sentence examples within Using Intracellular Recordings
Using intracellular recordings and neuronal labelings, we found that the Turkestan cockroach, Blatta lateralis, which is phylogenetically close to P.
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Using intracellular recordings, a sharp increase in the spontaneous neurotransmission in the levator auris longus muscle and a lower increase in the diaphragm muscle could be seen.
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Intracellular Recordings sentence examples within intracellular recordings revealed
Intracellular recordings revealed abnormal action potentials with depolarized resting potential, reduced upstroke velocity, prolonged repolarization, and effective refractory period in the ventricular preparations of Sorbs2 KO mice.
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Intracellular recordings revealed increases in respiratory‐related firing frequency and synaptic excitation of inspiratory protrudor and retractor hypoglossal motoneurones after CIH.
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This Cerebellar Classic is devoted to the pioneering work of Eccles, Llinás, and Sasaki on complex spikes using intracellular recordings from Purkinje neurons.
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Shifting the voltage-dependence and density of the T-channel conductance in a compartmental model replicates the burst properties from the intracellular recordings, pointing to molecular mechanisms that can generate burst diversity.
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Intracellular recordings in female flies show that blocking synaptic ACh output from either ACs or L4 neurons leads to slow repolarization of L1 and L2 neurons.
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Using in vitro intracellular recordings, we demonstrate that persistent firing is supported by individual neurons in hippocampal CA1 pyramidal cells through cholinergic receptor activation, but is dramatically attenuated by NA.
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Using a combination of pupillometry, extracellular, and intracellular recordings from identified LGN neurons in behaving mice, we show that thalamocortical (TC) neurons and interneurons are distinctly correlated to arousal forming two complementary coalitions.
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Intracellular recordings from bipolar cells in the salamander retina revealed frequent nonlinear integration of visual signals within bipolar cell receptive field centers, affecting the encoding of artificial and natural stimuli.
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Using a combination of pupillometry, extracellular and intracellular recordings from identified LGN neurons we show that thalamocortical neurons and interneurons are distinctly correlated to arousal forming two complementary coalitions.
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Intracellular recordings using sharp microelectrodes often rely on a technique called discontinuous current-clamp (DCC) to accurately record the membrane potential while injecting current through the same microelectrode.
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Here we use intracellular recordings in SI neurons to show that remote intracortical electrical perturbation can impact such constraints on the responses to given tactile input patterns.
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We performed laminar extracellular and intracellular recordings simultaneously from both barrel cortices in awake mice.
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In the present study, we performed intracellular recordings in the MGB of anesthetized guinea pigs and examined whether and how the TRN modulates the SSA of MGB neurons with inhibitory inputs.
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To study action potential (AP)-induced metaplasticity, we used intracellular recordings from CA1 pyramidal cells of rat hippocampal slices.
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We performed intracellular recordings from muscle of both genetic and pharmacologic mouse models of Becker disease to identify the mechanism underlying transient weakness.
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Over the past few decades, a great deal of information on the function of mammalian motor neurons (MNs) has been obtained from intracellular recordings collected in acute animal experiments.
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Intracellular recordings from CA3 pyramidal cells showed a more depolarised resting membrane potential in hA30P mice.
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In this study, we used extra- and intracellular recordings of extensor tibiae (ExtTi) and retractor coxae (RetCx) motor neurons (MNs) and identified local premotor nonspiking interneurons (NSIs), and mechanical stimulation of the fCO and tibial or trochanterofemoral CS (tiCS, tr/fCS), to investigate the premotor network architecture underlying multimodal proprioceptive integration.
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To further understand the contributions of these cellular mechanisms and to explore the involvement of LTCC subtypes in SCI-induced hyperexcitability, we measured root reflexes with ventral root recordings and motoneuron activities with intracellular recordings in an in vitro preparation using a mouse model of chronic SCI (cSCI).
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Intracellular recordings revealed abnormal action potentials with depolarized resting potential, reduced upstroke velocity, prolonged repolarization, and effective refractory period in the ventricular preparations of Sorbs2 KO mice.
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Fuzzy graphene and cellular optoporation enable intracellular recordings of cardiac action potentials on microelectrode arrays.
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Intracellular recordings revealed increases in respiratory‐related firing frequency and synaptic excitation of inspiratory protrudor and retractor hypoglossal motoneurones after CIH.
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In the past, intracellular recordings from the spinal and brainstem neurons were primarily made using sharp electrodes, although whole-cell patch-clamp technology has been around since the early 1980s.
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Using sharp electrode intracellular recordings in isolated, pressurized small pulmonary arteries from rats and mice, we show exposure to CH leads to PASMC membrane depolarization compared to control animals, and this is independent of intraluminal pressure-induced depolarization.
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We compare visual system evolution in three Heliconius butterfly species using a combination of intracellular recordings, ATAC-seq, and antibody staining.
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Isometric tension and intracellular recordings of endplate potentials from mouse muscles indicated that neither full-length nor truncated emGFP-TetC constructs significantly impaired NMJ function or transmission.
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Intracellular recordings of action potentials (APs) in individual fibers during depolarization revealed that APs do not fail in an all-or-none manner.
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Intracellular recordings in thalamic neurons reveal the 3-5 Hz oscillation to be associated with rhythmic low-threshold Ca2+ spikes.
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Results: The analysis of results indicated that (a) the signals appear more frequently in cells with higher RMP, (b) the depolarization amplitudes seem to be distributed randomly and have no correlation with other features, (c) hyperpolarization amplitudes show two distinct clusters and exhibit strong correlation with the RMP, and (d) time course of hyperpolarization phase shows no distinct groups and is distributed in a window larger than that of any other signals seen in the intracellular recordings.
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The enormous advances made over the last 50 years in materials science, microelectronics, and nanoelectronics, together with the acknowledgment that substrate-integrated planar multielectrode arrays (MEA) are limited to recording of extracellular field potentials (FPs) rather than the entire electrophysiological signaling repertoire of the brain, have prompted a number of laboratories to merge the advantages of planar MEA technologies (non-damaging and durable) with those of the classical sharp and patch electrodes for intracellular recordings.
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A comprehensive discussion of the data from electroencephalographic studies in DS humans and transgenic/trisomic mouse models is provided, as well as data from signals collected from local field potentials (LFP) and intracellular recordings in DS mouse models.
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Our model was initially based on modified Hodgkin-Huxley equations, adapted to represent variable, regenerative depolarisations (action potentials) we have occasionally observed in intracellular recordings and that can be triggered by excitatory junction potentials at neuromuscular synapses.
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We performed intracellular recordings from pairs of CGs and MB output neurons (MBONs) of male American cockroaches, the latter receiving synapses from Kenyon cells, and we found that a CG receives excitatory synapses from an MBON and that odor responses of the MBON are changed by current injection into the CG.
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Intracellular recordings of the neurons' activity in response to acoustic stimuli were obtained, while either the ipsilateral or the contralateral hearing organ was cold-deactivated.
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A similar decrease in the background noise with increasing C m was found in intracellular recordings in vivo.
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Systematic studies on neurons and cardiomyocytes show that controlling device curvature and sensor size is critical for achieving high-amplitude intracellular recordings.
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SUMMARY Intracellular recordings in vivo remains the best technique to link single-neuron electrical properties to network function.
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However, we have limited knowledge on subthreshold events that give rise to these responses, especially in non‐human primates, as intracellular recordings in awake animals pose substantial technical challenges.
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NEW & NOTEWORTHY To accurately observe the shape of action potentials, it is essential to perform intracellular recordings.
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Here we tested the utility of Magneto in the adult somatosensory cortex by performing whole-cell intracellular recordings in vitro and extracellular recordings in freely moving mice.
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Inhibitory input fields measured with patterned optogenetic stimulation and intracellular recordings revealed that these inputs originate from a wide range of orientation domains, inconsistent with a model of co-tuned inhibition and excitation.
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We validate these predictions with intracellular recordings from macaque retinal ganglion cells.
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Intracellular recordings were performed on Retzius neurons of the leech Haemopis sanguisuga using glass microelectrodes and input membrane resistance (IMR) was measured by injecting hyperpolarizing current pulses through these electrodes.
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Intracellular recordings were stable for several days, and monitoring of both basal activity as well as pharmacologically altered electric signals with high signal-to-noise ratios and excellent electrode coupling was performed.
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In vivo intracellular recordings showed that both SO and DO neurons had significantly shorter post-spike hyperpolarization latency and excitatory duration in response to CF-FM in comparison to CF stimuli, suggesting that the FM component had an inhibitory effect on the responses to the CF component.
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Intracellular recordings were used to measure subthreshold excitatory postsynaptic potentials (EPSPs) and suprathreshold action potentials (APs).
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Further, acquiring successful intracellular recordings is very tedious, requiring a high degree of skill not readily achieved in a typical laboratory.
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To understand the contribution of intrinsic membrane properties to the different in vivo firing patterns of oxytocin (OT) and vasopressin (VP) neurones, in vitro studies are needed, where stable intracellular recordings can be made.
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Intracellular recordings of the neurons’ activity in response to acoustic stimuli were obtained, while either the ipsilateral or the contralateral hearing organ was cold-deactivated.
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We further show through intracellular recordings that synaptic inhibition is likely to play a role in generating respiration-coupled oscillations at the membrane potential level.
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Since little is known about motion processing in these insects, we performed intracellular recordings from hawking, emerald dragonflies (Hemicordulia spp.
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Using visually guided intracellular recordings of somatosensory layer 2/3 pyramidal cells and interneurons in awake male and female mice, we measured the spectrum and size of voltage fluctuation and intrinsic cellular properties at different voltages.
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We then validated it by performing intracellular recordings from optogenetically-tagged amygdalar neurons followed by histological reconstruction, which revealed that it is accurate and precise to within ~100 μm.
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Here, using in vivo long-duration intracellular recordings at the corticostriatal synapses we show that EE promotes asymmetric anti-Hebbian STDP, i.
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OBJECTIVE
Nano neuroelectrodes, often adopting a nanoprotrusion structure, are promising for improved chronic reliability and capability of both extracellular and intracellular recordings.
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The authors also measured synaptic responses from the larval neuromuscular junction using sharp intracellular recordings, and performed Western blots to determine whether the truncated syntaxin1A is associated with presynaptic core complexes.
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We then validated it by performing intracellular recordings from optogenetically-tagged amygdalar neurons followed by histological reconstruction, which revealed that it is accurate and precise to within ∼100 μm.
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Intracellular recordings of postsynaptic potentials from motoneurons upon DCG-IV application demonstrated a decrease in the number of spikes and the overall area of responses evoked by dorsal root stimulation.
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Using intracellular recordings and neuronal labelings, we found that the Turkestan cockroach, Blatta lateralis, which is phylogenetically close to P.
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Effects of 5-HT1 and 5-HT2 receptor agonists and antagonists on membrane properties of motoneurons in the isolated lumbar segment of the frog spinal cord were investigated using intracellular recordings.
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Using intracellular recordings, a sharp increase in the spontaneous neurotransmission in the levator auris longus muscle and a lower increase in the diaphragm muscle could be seen.
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Here we tested the utility of Magneto in the adult somatosensory cortex by performing whole-cell intracellular recordings in vitro and extracellular recordings in freely moving mice.
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