Introduction to Fluorescence Tomography

using fluorescence lifetime imaging, fluorescence resonance energy transfer, fluorescence anisotropy, or fluorescence tomography.

The biomedical application’s potential in imaging modalities of functionalized microbubbles using a medical US device with a frequency of 50 MHz, fluorescence tomography, and raster-scanning optoacoustic mesoscopy measurements was evaluated.

Here we propose a label-free, automated, and ready-to-use 3D histological imaging technique, termed microtomy-assisted autofluorescence tomography with ultraviolet excitation (MATE).

The kinetics of all three agents were imaged for 1 h after administration using an MRI-coupled fluorescence tomography system.

Finally, images acquired by fluorescence tomography evidenced that the developed nanoplatform was effective in enabling nose-to-brain transport upon nasal administration.

The potential use of the obtained probes in biomedical applications was evaluated using fluorescence tomography, raster-scanning optoacoustic microscopy and ultrasound response measurements using a medical ultrasound device at the frequency of 33 MHz.

We develop a framework to register ex vivo images to histology using a novel cryo-fluorescence tomography (CFT) device.

We demonstrate its remarkable characteristics by imaging using a commercial photoacoustic/fluorescence tomography system (TriTom, PhotoSound Technologies).

The 3D electron density maps generated by PXCT were correlated with maps from X-ray fluorescence tomography and micro-Raman spectroscopy.

present an intravital mesoscopic fluorescence tomography (IFT) technique that is capable of tracking fluorescently labeled tumor cells inside living mice over several months.

We conclude that X-ray fluorescence tomography is very well suited to non-destructively characterize 3D nanomaterials with photonic and other functionalities.

, diffuse optical fluorescence tomography or positron emission tomography) or lack molecular sensitivity (e.

This work shows that hybrid microbubbles have all the necessary properties for multimodal imaging (ultrasound, raster-scanning microscopy, and fluorescence tomography), which demonstrate superior characteristics for potential theranostic and related biomedical applications.

Fluorous dyes soluble in FC oils are also key enablers for nascent whole-body imaging technologies such as cryo-fluorescence tomography (CFT).

Here, we propose chemical sectioning fluorescence tomography (CSFT) to solve this problem.

Herein, we examined the use of MRI-PAFT to estimate cancer surface receptor availability in response to anti-angiogenesis therapy, using MRI-coupled paired agent fluorescence tomography.

We propose to solve the inverse problem of diffuse fluorescence tomography (DFT) – reconstruction of the spatial distribution of the fluorophore in biological tissues – by a method based on Tikhonov regularisation with the nonnegativity condition (TRNC) of the reconstructed components of the solution vector.

X-ray excited fluorescence tomography is an emerging field in application of bio-imaging and computer tomography.

In this work, we investigate the inverse problem in k-space reflectance fluorescence tomography.

Confocal microscopy and fluorescence tomography imaging showed that the polymer-dye conjugate confers fluorescence properties to the NP suitable for further in vitro and in vivo pre-clinical studies.

Based on fluorescence tomography instead of tissue clearing, this method provides a simple, rapid way to precisely locate mRNAs in the whole brain with cytoarchitectonic landmarks.

By means of cryo soft X-ray tomography (cryo-SXT), which provides the cellular ultrastructure at 50 nm resolution, and cryo hard X-ray fluorescence tomography (cryo-XRF), which provides the elemental sensitivity with a 70 nm step size, we have located the iridium anticancer agent exclusively in the mitochondria.

Although this technique is demonstrated under the context of flame chemiluminescence tomography, it can be easily adapted for other tomographic modalities such as fluorescence tomography used in biomedical imaging to reduce the experimental costs.

A combination of gradient-magnetic-field controllable biodistribution of submicron carriers with fluorescence tomography/MRI imaging in vivo provides a new opportunity to improve drug delivery efficiency.

By combining X-ray fluorescence tomography and phase contrast nanotomography on the same cell with sub-100 nm pixel size, we establish a routine workflow from the data acquisition, data processing, to tomographic reconstruction.

PurposeThe purpose of this study was to show a systematic strategy for assessing the pharmacokinetics of indocyanine green (ICG)-loaded nanoparticles in the tumor tissue based on a dynamic diffuse fluorescence tomography (DFT) system.

Following intranasal administration in rats, brain targeting of the formulation was demonstrated by fluorescence tomography.

Immunofluorescence tomography is a high-resolution 3-D reconstruction method based on methacrylate embedding and serial-sectioning, where 2-D images of immuno-stained serial-sections are computationally aligned into image stacks, and the 3-D volume rendered.

Similarly to the bright field OPT bearing resemblance with the transmission x-ray computed tomography, the fluorescent mode OPT is analogous to x-ray fluorescence tomography (XFCT).

Diffuse optical fluorescence tomography (DOFT) systems in pre-clinical and diagnostic imaging applications have demonstrated improvements in fluorescence quantification with the addition of a priori data from structural imaging modalities (e.

Previously, we showed that MRI-coupled dual-agent fluorescence tomography (FMT) is capable of estimating the concentration of epidermal growth factor receptor (EGFR) in orthotopic glioma models noninvasively.

For example, the In Situ Nanoprobe (ISN, 19-ID), a next-generation hard x-ray nanoprobe, will use x-ray fluorescence tomography and ptychographic 3D imaging as key imaging modes with a spot size of 20 nm.

To examine these questions, we have developed a SWIR small animal fluorescence tomography system.

In this work, we develop a dual-modality imaging system combining three-dimensional (3D) fluorescence tomography with ultrasound (US) imaging.

Significance: Time domain fluorescence tomography, using zero cross-talk estimators, can serve as a powerful tool for quantifying multiple fluorescently labeled biological processes.

A hybrid system for intraoperative cone-beam CT (CBCT) imaging and continuous-wave fluorescence tomography (FT) has been developed using an image-guidance framework.

Here, we combine Intravital Fluorescence Tomography (IFT) and Spectral Domain Optical Coherence Tomography (SD-OCT) for tumor imaging with a Non-invasive Intravital Imaging Window (NIIW) for tissue stabilization.

We have developed the Smart ToolkIt for Fluorescence Tomography (STIFT) software platform for simulating, reconstructing and optimizing FMT.

While generally applicable, we demonstrate our method on x-ray fluorescence tomography from a tissue specimen and compare the reconstruction quality with conventional methods.

As one of the most sensitive in vivo molecular imaging modalities, Fluorescence Tomography plays an essential role in preclinical studies.

Using a novel combination of nanoscale-resolution 3D cryogenic synchrotron-light ptychography, holotomography and fluorescence tomography, we show how soil bacteria interact with Arabidopsis thaliana and promote the uptake of various metals.