Introduction to Fluorescence Spectrophotometry

Binding parameters of the interaction between the two full-length proteins, VcFtsA, and VcFtsZ determined by fluorescence spectrophotometry yielded a Kd value of around 38 μM.

In this study, the detection of three antibiotics (enrofloxacin, oxytetracycline and sulfamethoxazole) and a β-agonist (ractopamine) was carried out using fluorescence spectrophotometry, with a semi-quantitative approach and a low environmental impact.

Methods The RADA16-I–MA suspension was prepared by magnetic stirring, followed by fluorescence spectrophotometry, particle size determination, rheological properties analysis, and in vitro release assay to characterize the interaction between RADA16-I and MA.

Moreover, the prepared hybrid frameworks were screened against various anions using UV-Visible absorption and fluorescence spectrophotometry.

Advances in synthesis and tailoring of functional nanomaterials has allowed for their use in fluorescence spectrophotometry, surface-enhanced Raman spectrometry (SERS), and in electrochemical based sensor systems for different analytical applications.

The formation of AGEs and four kinds of dicarbonyl compounds (glyoxal, methylglyoxal, 3-deoxyglucosulose, 2,3-butanedione) were determined by fluorescence spectrophotometry and HPLC, respectively.

The purpose of this study was to observe the interaction between different self-assembling peptides and emodin through fluorescence spectrophotometry, CD, SEM and AFM; to construct a preliminary suspension in-situ hydrogel delivery system for emodin with the self-assembling peptides; and to investigate the drug-loading and drug-releasing properties of the self-assembling peptides on emodin.

Methods: the use of self-assembly technology to prepare polypeptide / Chit2DC (chitosan - deoxycholate) drug micelles, transmission electron microscopic morphology, fluorescence spectrophotometry to calculate the loading rate, drug loading, and drug release rule.

Plasma was collected for vitamin C, measured by spectrophotometry; and alpha- and gamma-tocopherol, retinol, and malondialdehyde—a marker of oxidative stress—using high pressure liquid chromatography and fluorescence spectrophotometry.

Synthesized GQDs were characterized by UV–visible spectroscopy, FTIR, Transmission electron microscopy, fluorescence spectrophotometry.

In the present study, we used behavioral tests, Western blotting, double-immunofluorescence labeling, and fluorescence spectrophotometry to investigate whether acupuncture performed at acupoints “Baihui” (GV20) and “Yintang” (GV29) affected behavioral stereotypies and regulated the dopamine (DA) system in three different brain regions in Balb/c mice injected with 3,3′-iminodipropionitrile (IDPN) as a model for TS.

The luminescent properties of PLA/Tb(SSA)3Phen composite films were investigated by fluorescence spectrophotometry.

The nature of the host–guest interactions was further examined by fluorescence spectrophotometry, whereby sCX[8] quenched the fluorescence of phenylalanine, both as the free amino acid, and as a residue within the KLVFFA peptide, at host–guest ratios of 1.

Three fluorescent dyes (DAPI, Alexa Fluor 488, and Alexa Fluor 594) and a fluorescence spectrophotometry reader were used, which confirmed that the mutual interference of the three fluorescent dyes is very low.

A novel magnetic graphene oxide modified with Au nanoparticles (Fe3O4@CS/GO/Au) as an efficient adsorbent was prepared for the detection of rhodamine B (RB) coupled with fluorescence spectrophotometry.

The present study aimed to develop a fluorescence spectrophotometry approach for the fast screening of MOAH in grains.

Blood samples from common carotid artery were obtained at 6 h after establishing the model for determination of concentrations of epinephrine (E) and norepinephrine (NE) in plasma (by enzyme-linked immunosorbent assay), concentrations of FITC-Dex in serum (using fluorescence spectrophotometry) and activity of diamine oxidase (DAO) in serum (by colorimetry).

We have investigated the reaction of isolated MRPs from an asparagine–glucose model system with hemoglobin (Hb) to elucidate the binding effect of the MRPs in hemoglobin by fluorescence spectrophotometry.

The images obtained by fluorescence microscopy and the data from fluorescence spectrophotometry using propidium iodide (PI) demonstrated alterations in the permeabilization of the cytoplasmic membrane of P.

ABSTRACT We used UV-vis absorption spectroscopy, fluorescence spectrophotometry and molecular docking calculations to investigate intermolecular interaction between the cationic dye, Nile blue (NB), and synthetic polynucleotides, poly(A-T), poly(G-C) and calf thymus DNA (Ct-DNA) at physiological pH.

The optical properties of N, Zn-CDs were investigated using UV-Vis and fluorescence spectrophotometry and also the N, Zn-CDs structural features were studied with other characterization tools like XPS, TEM, EDX, FTIR and XRD.

The samples were characterized by Absorption Spectrophotometry, Fluorescence Spectrophotometry, Particle Size Analysis and SEM Analysis.

The fluorescence DNA probe was released on surface of AuNPs and the fluorescence intensity was read by fluorescence spectrophotometry.

A series of gradient constitutive promoters from Escherichia coli were randomly cloned upstream of pathway genes, and the resulting library was screened using an ultraviolet spectrophotometry–fluorescence spectrophotometry high‐throughput method, which was established based on the interactions between AlCl3 and (2S)‐naringenin.

Fluorescence properties of BC-CQD solution were assessed by fluorescence spectrophotometry.

Fluorescence spectrophotometry and confocal microscopy showed B.

The critical concentration of the hydrophobic association was determined using fluorescence spectrophotometry.

He demonstrated cellular NADH auto-fluorescence in 1958 by fluorescence spectrophotometry (1).

We constructed and validated a novel emulsion PCR method combined with fluorescence spectrophotometry (EPFS) for simultaneous qualitative, quantitative and high-throughput detection of multiple DNA targets.

Fluorescence spectrophotometry analysis indicated that hydroxyl free radicals could be continuously produced when using rutile TiO2 as the photocatalyst.

aureus (101 to 108 CFU mL-1) by fluorescence spectrophotometry.

Fluorescence spectrophotometry was used to assess reagent bio-distribution, while Western blots and immunohistochemistry were used to assess the localization of the apoptotic markers Bcl-2, Bax, and cleaved CASP3 in the LAA.

Two metabolite classes were studied, amines (quantified by high-performance liquid chromatography and fluorescence spectrophotometry) and flavonols (quantified by ultra-high-performance liquid chromatography with triple quadrupole mass spectrometry).

MMIPs and CDs@VPA were characterized by TEM, particle size analysis, FT-IR, VSM, XPS, adsorption experiments, and fluorescence spectrophotometry in turn.

Fluorescence spectrophotometry based on SYBR Green signal and GOX as the fluorescence quencher was used for detection and quantification of targets’ miRNAs and change in fluorescence intensity due to absence and presence of the targets was measured.