Introduction to Fluorescence Nanoscopy

The presented methodology is able to image biological samples competing current fluorescence nanoscopy but at less interference due to the low energy of infrared radiation and the absence of labeling.

Fluorescence nanoscopy allows to non-invasively resolve three-dimensional cell structures beyond the diffraction limit [1].

We report a comparison of two photonic techniques for single-molecule sensing: fluorescence nanoscopy and optoplasmonic sensing.

Here, we investigate the parameters of high-professional scientific- and amateur-grade cameras in the view of their use in the fluorescence nanoscopy with the detection of single molecules and, in general, quantum emitters of different nature.

Fluorescence nanoscopy provides imaging techniques that overcome the diffraction-limited resolution barrier in light microscopy, thereby opening up a new area of research in biomedical imaging in fields such as neuroscience.

The classic view of organelle cell biology is undergoing a constant revision fueled by the new insights unraveled by fluorescence nanoscopy, which enable sensitive, faster and gentler observation of specific proteins in situ.

With a 3D precision as high as 2 – 3 nanometers, 4Pi-STORM achieves new levels of image detail, and extends the range of biological questions that can be addressed by fluorescence nanoscopy, as we demonstrate by investigating protein and nucleic acid organization in primary neurons and mammalian mitochondria.

Fluorescence nanoscopy using single-molecule localization microscopy shows that they have very good photophysical properties and a remarkable blinking behaviour at 405 nm.

This chapter discusses the simple yet powerful principles that make it possible to neutralize the resolution-limiting role of diffraction in far-field fluorescence nanoscopy methods such as STED, RESOLFT, PALM/"​"​STORM, or PAINT.

Recent advances in fluorescence nanoscopy have revolutionized direct investigation of presynaptic release organization and dynamics.

Live-cell fluorescence nanoscopy is a powerful tool to study cellular biology on a molecular scale, yet its use is held back by the paucity of suitable fluorescent probes.

Applying super-resolution fluorescence nanoscopy techniques may provide a solution for both rapid inspection and defect identification capable of inspecting hundreds of wafers per hour.

We present a 3D fluorescence nanoscopy method operating at cryogenic temperatures, based on optical saturation of the excited state of individual molecules.

Herein, we took advantage of the latest developments in atomic force imaging and super‐resolution fluorescence nanoscopy to define the organization of the supramolecular complex(es) that surround the fenestrae.

Live-cell fluorescence nanoscopy is a powerful tool to study cellular biology on a molecular scale, yet its use is held back by the paucity of suitable fluorescent probes.

However, the methods of fluorescence nanoscopy are still poorly suitable for studying living cells.

RESOLFT fluorescence nanoscopy can nowadays image details far beyond the diffraction limit.

We analyze their optical properties, including emission wavelength, photon-statistics and photodynamics, and show that they are suitable for far-field super-resolution fluorescence nanoscopy.