Introduction to Fluorescence Identification

We aimed to explore and establish a membrane-preserving method for immunofluorescence identification of CTCs that keeping the isolated cells intact.

3) Immunofluorescence identification of the cells showed that most of the two kinds of cells in the second passage were CK19 positive and were epithelial cells, while most of the cells in the fifth passage expressed positive vimentin antibody and were stroma cells.

In addition, the fluorescence identification mechanism was deduced to be a redox reaction between the sensor and NAD(P)H according to the fluorescence behavior.

A novel assay was developed here using fluorescence identification of β-lactamase activity (FIBA) to permit rapid detection and classification of bacterial carbapenemases.

While we recommend performing standard diagnostics along with fluorescence, our study supports the use of visual UV fluorescence identification as a preliminary, affordable, noninvasive, and field-applicable method to screen populations for ophidiomycosis.

DEGs with a prognostic value considered as candidate genes were evaluated, followed by genetic analysis of tumor infiltrating immune cells based on Wilcoxon test and immunofluorescence identification.

MF-MIPs sensor exhibited a high fluorescence response and selectivity based on the dual-recognition mechanism of imprinting recognition and fluorescence identification.

This makes the MIP@CQD-PCM achieve an enhanced capability for the fluorescence identification of tetracycline in a high-temperature environment through the in situ thermal management of its PCM core.

We successfully demonstrated label-free fluorescence identification of collagen type I and II – isolated in cartilage here for the first time and the co-enzymes free NADH and FAD which together give the optical redox ratio that is an important measure of metabolic activity.

Result shows that they are not significantly different at the confidence level of 95% and 98%, which proves fluorescence identification has higher accuracy.

The synthesized diarylethene displayed excellent selectivity to Al3+ with a distinct fluorescence change, revealing that it could be used as a sensor for fluorescence identification of Al3+, and a logic circuit was constructed by utilizing this diarylethene molecular platform.

Immunofluorescence identification of CTC-3 were DAPI-positive, Cytokeratin-positive, and CD45-negative.

However, many existing methods for CTC detection commonly rely on conventional three-color immunofluorescence identification, which damages CTCs and easily causes loss of cells.