Introduction to Fluorescence Detector

An analytical method was developed for determining vitamin K1 and vitamin K2 in foods by high performance liquid chromatography with Fluorescence Detector(HPLC-FLD).

A novel method based on ultrasonic-assisted aqueous two-phase extraction (UAATPE) coupled with solidifying organic drop-dispersible liquid–liquid microextraction (SOD-DLLME) was developed for simultaneous determination of nine mycotoxins (aflatoxins of B1, B2, G1, G2, and M1, ochratoxin A, zearalenone, deoxynivalenol and patulin) in medicinal and edible foods by high-performance liquid chromatography (HPLC) with diode array detector (DAD) and fluorescence detector (FLD) in series.

Urinary 1-AP and atmospheric nitro-PAH levels were measured using a high-pressure liquid chromatography-fluorescence detector (HPLC-FD).

An analytical method was validated for determination of USEPA's listed 16 polycyclic aromatic hydrocarbons' (PAHs) in cooked chicken and roasted coffee using high pressure liquid chromatography-fluorescence detector (HPLC-FLD) with a C18 column.

The proposed measurement setup consists of a UV–VIS diode-array detector and a fluorescence detector.

Based on chemical structure and spectroscopic properties, chromatographic behaviors of common antidiabetics were further studied under different HPLC conditions combining with multi-wavelength monitoring with diode array detector (DAD) and fluorescence detector (FLD) to highlight and discriminate illegal adulterants in BMGSG products.

Abbreviations: MAM: multi-attribute method; PQAs: product quality attributes; CE: capillary electrophoresis; IEX: ion-exchange chromatography; HILIC-FLR: hydrophilic interaction liquid chromatography coupled to a fluorescence detector; RP-LC/UV: reversed-phase liquid chromatography coupled to a UV detector; MS: mass spectrometry; NPD: new peak detection; GdnHCl: guanidine hydrochloride; TIC: total ion current; pAb: polyclonal antibody; IgG: immunoglobulin G; DTT: dithiothreitol; IAA: iodoacetic acid; TFA: trifluoroacetic acid; A280: absorbance at 280 nm wavelength; 96MPH: 96-channel multi-probe head; CPAC: Cold Plate Air Cooled; HHS: Hamilton Heater Shaker; DWP: Deep-Well Plate; PCR: Polymerase Chain Reaction; NTR: Nested Tip Rack; Met: methionine; Trp: tryptophan; N-term pQ: N-terminal glutamine cyclization; Lys: lysine; PAM: peptidylglycine α-amidating monooxygenase; G0F: asialo-, agalacto-, bi-antennary, core substituted with fucose; G1F: asialo-, mono-galactosylated bi-antennary, core substituted with fucose; G2F: asialo-, bi-galactosylated bi-antennary, core substituted with fucose; G0: asialo-, agalacto-, bi-antennary; Man5: oligomannose 5; Man8: oligomannose 8; TriF: asialo-, tri-galactosylated tri-antennary, core substituted with fucose.

The composition profiles of clove buds essential oil and hydrosoluble polyphenols obtained during in-situ microwave assisted extraction (IMWAE) were analysed and quantified by head space gas chromatography mass spectrometry (HS-GC–MS) and liquid chromatography with UV/visible diode array/fluorescence detector (HPLC-DAD-FD).

A micro-capillary electrophoresis (μCE) system is one of the widely adopted techniques in the molecular diagnostics and DNA sequencing due to the benefits of high resolution, rapid analysis, and low reagent consumption, but due to the requirements of bulky high-power suppliers and an expensive laser-induced fluorescence detector module, the conventional set-up of μCE system is not adequate for point-of-care (POC) molecular diagnostics.

A laser-induced fluorescence detector coupled with an interface was applied to detect the targets on the paper-based chip.

The Telescope Array (TA) experiment has three fluorescence detector(FD) stations to detect fluorescence emission by extensive air showers induced by Ultra High Energy Cosmic Rays(UHECRs).

The Telescope Array (TA) is a hybrid cosmic ray detector deployed in 2007 in Millard County, Utah, USA, which consists of a surface detector of 507 plastic scintillation counters spanning a 700 km2 area on the ground that is overlooked by three fluorescence detector stations.

Following the evidence for a hotspot in the arrival directions of the highest energy cosmic rays, the Telescope Array (TA) Experiment undertook the TAx4 upgrade to expand the area of our Surface Detectors (SD) by a factor of 4 and have added new Fluorescence Detector (FD) stations to view over the new SD arrays.

The Cu-MOF-74 fluorescent probe exhibited excellent anti-interference ability, high selectivity, good fluorescence intensity stability in the short term, so it can be used as a reliable fluorescent probe for the highly specific detection of low concentration ATMP, the revealed microscopic mechanism of Cu-MOF-74 fluorescent probe to ATMP from the atomic level can provide a theoretical basis for the research of new fluorescence detectors.

Significantly, the electron-deficient Zr-MOF itself, as a kind of high-sensitivity fluorescence detector, has a unique fluorescence "turn-on" effect with 5-Fu.

High-performance liquid chromatography and a high-sensitivity fluorescence detector were used for detection, in which a fluorescence spectrophotometer was used to determine the maximum excitation wavelength and emission wavelength of2H-isoindole-1-sulfonate.

An improved sensitive procedure was also evolved for tramadol using a fluorescence detector system.

Twenty European students benefited from a week's training with theoretical and practical sessions in six complementary approaches: (1) analytical ultracentrifugation with or without a fluorescence detector system (AUC-FDS), (2) isothermal titration calorimetry (ITC), (3) size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), (4) bio-layer interferometry (BLI), (5) microscale thermophoresis (MST) and, (6) switchSENSE.

Quantification was performed on a fluorescence detector set at excitation 275 nm, emission 313 nm and bisphenol-B was used as internal standard.

2 mL/min and the detection was performed with a fluorescence detector set at 417 nm (λ emission ) after excitation at 254 nm (λ excitation ).