Introduction to Fluorescence Colocalization

Using a single-molecule fluorescence colocalization experiment, we measured the thermodynamic and real-time kinetic properties of fluorescence-labeled Gram-positive bacteria Geobacillus stearothermophilus PriA binding on DNA forks.

This was also confirmed by fluorescence colocalization.

Hence, we propose here an optical super-resolution imaging based immunoassay strategy, named super-resolution multicolor fluorescence colocalization (SR-MFC), which can generate a low false-positive rate.

Here, we introduce an approach combining cell-derived nanovesicles, microfluidics, and single-molecule fluorescence colocalization microscopy to track individual binding events at a cyclic nucleotide-gated TAX-4 ion channel critical for sensory transduction.

Fluorescence colocalization and cell uptake assays were performed to elucidate the correlation between the selected binding protein and RRL, and the internalization mechanism of RRL.

Immunofluorescence colocalization exhibited that the regulating action of Gyp-17 may focus on Müller cells.

Immunofluorescence colocalization analysis revealed that S100P was mainly distributed in the epithelium.

Immunofluorescence colocalization of GFP with CD45 pan‐leukocyte and F4/80 macrophage markers corroborated these findings.

Fluorescence colocalization studies demonstrated that these proton-sponge DSSF poly(β-amino esters) targeted mitochondria through electrostatic interactions.

Co-immunoprecipitation (co-IP), yeast two-hybrid (Y2H) and immunofluorescence colocalization assays were used to confirm the PPI.

The fluorescence colocalization imaging proved an accumulated effect of astaxanthin encapsulated in casein emulsion nanocarriers modified by TPP.

To further characterize which cells within the olfactory mucosa were invaded by the virus, they performed immunofluorescence colocalization studies.

Mechanistic studies (Bioinformatics binding site analyses, the Luciferase reporter, Ago2 immunoprecipitation, the RNA pull-down, immunofluorescence colocalization, rescued assays and western blotting) implicated that RP11-51O6.

We discovered an interaction between CIRBP and ELAVL1 using STRING software, which was verified through co‐immunoprecipitation and fluorescence colocalization assays.

The fluorescence colocalization images of mitochondria and lysosomes demonstrated that SeNPs significantly reduced the fusion of mitochondria and lysosomes when compared to the oxidative stress model group.

Multi-wavelength single-molecule fluorescence colocalization (CoSMoS) methods allow elucidation of complex biochemical reaction mechanisms.

Here we demonstrate that the efficient cancer suppression with the FA-displaying exosome was due to the receptor-mediated cytosol delivery of the siRNA payload without endosome trapping, as attested by fluorescence colocalization analysis, gene knockdown assay and animal tumor regression.

With the use of live cell single‐molecule super‐resolution imaging, Förster resonance energy transfer and nanoscale total internal reflection fluorescence colocalization microscopy, we visualized toxin pore formation in the presence of its natural docking ligand.

Immunofluorescence colocalization and gene correlation analysis confirmed that α5 was mainly expressed in cancer‐associated fibroblasts (CAFs).

Immunofluorescence colocalization with AKT was implemented by a click reaction of the SW probe and alkynyl CY5.

In addition, we analyzed the subcellular localization of SVCT2 by immunofluorescence colocalization assays and cellular fractionation experiments.

Quantification of fluorescence colocalization and intensity of strongly overlapping cells, e.

Confocal microscopy was used to determine the distribution of these proteins in the cells, and immunofluorescence colocalization analysis was used to evaluate the relationship between IFITMs and the CSFV endosomal pathway, including early endosomes, late endosomes, and lysosomes.

Immunofluorescence colocalization and microscale thermophoresis analysis showed that TAGLN2 directly binds to actin.

Salmonella infantis infection also resulted in mitochondrial damage and impaired mitophagy in the ileum and IPEC‐J2 cells, as demonstrated by immunofluorescence colocalization of mitochondria with microtubule‐binding protein light chain 3 (LC3) and high expression of autophagy‐related proteins PTEN‐induced putative kinase 1 (PINK1), sequestosome 1 (SQSTM1/p62), optineurin (OPTN), and LC3 by Western blotting analysis.

How overexpression of Sirt3 promoting diabetic corneal epithelial wound healing was investigated with cell migration assay immunofluorescence immunofluorescence colocalization and corneal injury model.