Introduction to Agar Dilution

What is/are Agar Dilution?

Agar Dilution - hydrophila SS332 were measured by the agar dilution method. ^[1] Antibacterial test using broth and agar dilution method was performed in a total of 5 treatment groups with 6 repetitions. ^[2] 25 µg/ml, determined by agar dilution. ^[3] Fosfomycin and amikacin MICs were assessed by agar dilution (AD), gradient strip assay (GSA), and broth microdilution (BMD). ^[4] fragilis in comparison with agar dilution with EUCAST methodology and breakpoints. ^[5] 625% by agar dilution assay. ^[6] Antibiotic susceptibility testing was performed using an agar dilution method. ^[7] The in vitro antibacterial activities against drug-sensitive and drug-resistance bacteria were evaluated by agar dilution method. ^[8] Methods The MICs of ertapenem on 503 Neisseria gonorrhoeae isolates (415 isolates selected randomly and 88 isolates selected with preference) were measured using the agar dilution method. ^[9] difficile using the agar dilution susceptibility testing method. ^[10] Disk diffusion and agar dilution methods were used to determine antibiotic susceptibility patterns. ^[11] MIC of imipenem, colistin and amikacin were done by agar dilution method. ^[12] Then, the MIC of different combinations of honey, ciprofloxacin, and plant alkaloid extract and expression of acrA and soxS genes were carried out using the agar dilution method and quantitative RT- PCR methods. ^[13] Agar dilution was performed to determine MIC of vancomycin and mupirocin. ^[14] Antibacterialactivity of extracts were determined using disk diffusion, agar dilution methods. ^[15] Antimicrobial activities of plant extracts were determined against the test microorganisms using the agar dilution method. ^[16] Their antimicrobial activity was assayed through in vitro models such as agar dilution assay. ^[17] We tested the susceptibility of these strains to sitafloxacin, and other antibiotics for Helicobacter eradication, including clarithromycin (CLR), metronidazole (MTZ), amoxicillin (AMX), tetracycline (TET), levofloxacin (LEV), and ciprofloxacin (CIP) using the agar dilution technique. ^[18] Which were used including gram-positive, gram-negative bacteria and fungus by agar dilution method. ^[19] Their antimicrobial sensitivities to were determined using agar dilution methodology (CLSI; M11-A7). ^[20] The synthesized derivatives were evaluated in vitro for antibacterial and antifungal activities using the agar dilution method. ^[21] globossum were tested against standard bacteria and fungus strains by agar dilution method. ^[22] Among 4,511 isolates, we found ≥95% concordance (within one doubling dilution) between AST performed locally using Etest® compared to agar dilution (reference method) for ceftriaxone, cefixime and azithromycin. ^[23] Antimicrobial activity of plant extract and fractions against strains of diarrhoeagenic Escherichia coli, Salmonella cholerae-suis, Shigella dysenteriae, Proteus mirabilis and Acinetobacter baumannii was determined by agar well diffusion and MIC by agar dilution methods. ^[24] The activity of omadacycline and five comparators was assessed with agar dilution on a 2015-to-2018 collection of 65 C. ^[25] pneumoniae isolates from sputum and blood were analyzed for serotyping by the Quellung reaction and for antimicrobial susceptibility by the agar dilution method and e-test and for multilocus sequence typing. ^[26] faecalis KZN that remained unchanged in agar dilution. ^[27] Methodology We sourced 394 NAS isolates from clinical samples, healthy human volunteers, animals and type cultures and subjected them to agar dilution susceptibility testing against eight antimicrobials. ^[28] fragilis-group isolates- and compared to agar dilution MICs. ^[29] , Asteraceae, leaves were tested by the agar dilution method against sixteen bacterial strains acquired from hospital infection sites and 12 strains from the American Type Culture Collection. ^[30] Agar dilution (AD), as recommended by EUCAST, was used as an alternative reference method to arbitrate discrepancies or address technical issues. ^[31] Ethanolic leaf extracts of Ocimum gratissimum and Moringa oleifera were evaluated for in vitro antifungal activities on Aspergillus and Fusarium species isolated from spoilt tomatoes and banana using the Agar Dilution Method. ^[32] In addition, mcr 1 and 5 positive isolates were phenotypically resistant to colistin using the agar dilution assay (> 8ug/ml). ^[33] OBJECTIVE To compare the performance of agar dilution and broth microdilution by commercial and in-house prepared plates for the Bacteroides fragilis group. ^[34] All the synthesized compounds were screened for their antitubercular, anti-HIV and antibacterial activity against different gram positive and gram negative strains by agar dilution method. ^[35] In this study, using BMD as a reference method, the performance of agar dilution (AD) and MIC test strips (MTS) were evaluated in polymyxin B susceptibility testing. ^[36] MICs were determined using the agar dilution method with Mueller-Hinton agar according to Clinical and Laboratory Standards Institute (CLSI) recommendations. ^[37] pylori isolates and FFPE tissues were tested for susceptibility to amoxicillin, clarithromycin, metronidazole, levofloxacin, tetracycline and rifabutin using agar dilution and NGS targeted to 23S rRNA, gyrA, 16S rRNA, pbp1, rpoB and rdxA. ^[38] Clarithromycin resistance was assessed by the agar dilution method. ^[39] Antimicrobial susceptibility was evaluated using the broth microdilution and agar dilution methods. ^[40] The antimicrobial activity of tea tree oil was evaluated using agar dilution method recording MIC50 value of 12. ^[41] METHODS The antimicrobial susceptibility tests were performed according to the agar dilution method. ^[42] Sensitivity to antibiotics was determined using the agar dilution method. ^[43] The antifungal activity of the target compounds was preliminarily evaluated by agar dilution method. ^[44] A rhamnose-degrading bacterium, strain BoRhaAT, was isolated from profundal sediment of Lake Constance in agar dilution series with l-rhamnose as substrate and with a background lawn of Methanospirillum hungatei. ^[45] The antifungal and anti-conidiogenic activities were evaluated by the agar dilution method. ^[46] aeruginosa was detected by biochemical and molecular (detection of pbo1 gene by colony PCR method) methods and the MIC and MBC of AgNPs were determined by agar dilution method. ^[47] Carbapenem and fosfomycin susceptibility were tested by Etest (bioMerieux, France) and agar dilution methods, respectively and evaluated in accordance with EUCAST criteria. ^[48] Susceptibility was evaluated by agar dilution using standard Mueller-Hinton agar (Pi concentration of 1mM similar to human plasma concentration) and supplemented with Pi (13 and 42mM, minimum and maximum urinary Pi concentrations) and/or glucose-6-phosphate (25mg/L). ^[49] The linezolid and tedizolid susceptibility were determined by agar dilution. ^[50]

minimum inhibitory concentration

Aim: To determine the fosfomycin sensitivity pattern along with Minimum Inhibitory Concentration (MIC) against uropathogens by agar dilution method. ^[1] Minimum inhibitory concentrations (MICs) were determined using agar dilution, following CLSI guidelines (CLSI, 2012; 2017). ^[2] Agar well diffusion and agar dilution methods were used to evaluate antibacterial activities and minimum inhibitory concentrations (MIC). ^[3] The minimum inhibitory concentration (MIC) of the active essential oil was determined using Agar Dilution Method. ^[4] NAAT-positive men were recalled and a urethral swab performed for gonococcal culture using modified Thayer-Martin media with determination of minimum inhibitory concentrations (MICs) by agar dilution. ^[5] flavus, determined by the agar dilution method, with the minimum inhibitory concentration (MIC) of 12. ^[6] The minimum inhibitory concentration was performed by the agar dilution method, while the minimum biofilm eradication concentration was performed by the broth dilution method. ^[7] This study aimed to evaluate the minimum inhibitory concentrations of three natural antimicrobial compounds—chitosan, ethanolic propolis extract, and nisin—against 37 microorganisms (different pathogens and spoilage microorganisms) by the methods of agar dilution and drop diffusion on agar. ^[8] Methods The minimum inhibitory concentration (MIC) levels were determined by the agar dilution method, and polymerase chain reaction was conducted to analyze the distribution of florfenicol resistance genes in 39 CoNS strains isolated from poultry and livestock animals and seafood. ^[9] Methodology: Minimum inhibitory concentrations of antibiotics were determined by agar dilution or broth microdilution method. ^[10] The gentamicin minimum inhibitory concentration (MIC) was determined using agar dilution method with an antibiotic dilution range of 0. ^[11] The minimum inhibitory concentration (MIC) of colistin for each CRAB isolate was determined using the broth dilution method, whereas those of sulbactam and fosfomycin were determined using the agar dilution method. ^[12] The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined using agar dilution technique. ^[13] In vitro antitubercular activity was carried out on Mycobacterium tuberculosis H37Rv (ATCC27294) strain using the agar dilution method, and minimum inhibitory concentration (MIC) was determined. ^[14] For the determination of the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), the agar dilution method was used. ^[15] Minimum inhibitory concentrations (MICs) of amoxicillin, piperacillin, cefotaxime, imipenem, clindamycin, vancomycin, linezolid, moxifloxacin, rifampicin, and metronidazole were determined by the reference agar dilution method. ^[16] The minimum inhibitory concentrations (MIC) were determined using the agar dilution method as per Clinical and Laboratory Standards Institution guidelines. ^[17] pylori were evaluated by agar dilution method, and the corresponding MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration) values were determined for each sample. ^[18] METHODOLOGY Determination of the minimum inhibitory concentration (MIC): The antimicrobial activity of propolis and essential oils was investigated by the agar dilution method. ^[19] Minimum inhibitory concentrations (MICs) were determined by an agar dilution method and bactericidal activity was evaluated by a time-kill assay. ^[20] Colistin resistance was determined by minimum inhibitory concentration (MIC) using agar dilution method. ^[21] Materials and Methods: The minimum inhibitory concentration (MIC) of CHE against CRSM was determined using the agar dilution method. ^[22] The antibacterial activity of CrAgNPs was analyzed by Agar dilution, Minimum Inhibitory Concentration methods. ^[23] The minimum inhibitory concentration of the extract was determined against the isolated microorganism by agar dilution method. ^[24] Minimum inhibitory concentrations (MICs) of the plant extracts against selected pathogens were also assessed using the agar dilution method. ^[25] Minimum inhibitory concentrations (MICs) of seven antimicrobials were determined by the agar dilution method. ^[26] gonorrhoeae strains were collected, and the minimum inhibitory concentrations (MICs) of various antibiotics were assessed for each strain using the agar dilution method. ^[27] The minimum inhibitory concentrations (MICs) of NIT were detected by agar dilution method. ^[28] Therefore, we determined the minimum inhibitory concentrations (MICs) of sitafloxacin and comparators by broth microdilution or agar dilution method against 1101 clinical isolates collected from 2017 to 2019 in 31 hospitals across China. ^[29] The minimum inhibitory concentration (MIC) of TGC to clinically isolated AB was determined by broth microdilution method, agar dilution method, and disk diffusion method. ^[30] Colistin resistance was determined by minimum inhibitory concentration (MIC) using agar dilution method. ^[31] Antimicrobial activity was assessed by agar well-diffusion method and the Minimum Inhibitory Concentration (MIC) by agar dilution method against six bacteria, Candida albicans, and Botrytis cinerea. ^[32] Methods 407 isolates from Nanjing, China, obtained in 2016 and 2017, had minimum inhibitory concentrations (MICs) determined for gentamicin using the agar dilution method. ^[33] Minimum inhibitory concentration (MIC) of vancomycin for MRSA was established by agar dilution method and chromosomal DNA was extracted and used in polymerase chain reaction targeting the mecA and vanA genes. ^[34] The minimum inhibitory concentrations (MICs) of clindamycin, erythromycin, azithromycin, tetracycline and doxycycline were determined by agar dilution technique recommended by Clinical and Laboratory Standards Institute (CLSI) for anaerobic bacteria. ^[35] Therefore, we determined the minimum inhibitory concentrations (MICs) of sitafloxacin and comparators by broth microdilution or agar dilution method against 1101 clinical isolates collected from 2017 to 2019 in 31 hospitals across China. ^[36] Objective: To compare the in-vitro efficacy by determining Minimum Inhibitory Concentration of Vancomycinusing the reference Agar Dilution to the E-Strip in Methicillin Resistant Staphylococcus aureus isolates. ^[37] An agar dilution test was used to determine the minimum inhibitory concentration and susceptibility profiles against doxycycline, ciprofloxacin, nalidixic acid, enrofloxacin, chloramphenicol and erythromycin. ^[38] Colistin resistance was determined by minimum inhibitory concentration (MIC) using agar dilution method. ^[39] Agar well diffusion, agar dilution and spread plate methods were employed to determine the zone of inhibition, Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and rate of killing respectively. ^[40] The Minimum Inhibitory Concentration (MIC) of the most potent honey was determined using the agar dilution method. ^[41] Minimum inhibitory concentrations (MICs) for gepotidacin and 6 comparators were determined by agar dilution (AD) for all isolates, and by both AD and broth microdilution (BMD) for Bacteroides spp. ^[42] The minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) were determined using agar dilution method while the mode of action of the active fractions was investigated through time-kill dynamics and leakages of proteins and potassium ions from the cells. ^[43] Minimum inhibitory concentration for Zink O and Silver O nanoparticles was detected by using standard agar dilution method. ^[44] Minimum inhibitory concentrations were determined using agar dilution with multipoint inoculation. ^[45] Minimum inhibitory concentration (MIC) of aqueous and ethanolic solution was obtained by using broth dilution method and agar dilution method. ^[46] Minimum inhibitory concentrations (MIC) were determined by agar dilution and interpreted according to EUCAST breakpoints. ^[47] The minimum inhibitory concentrations were determined by agar dilution test. ^[48] The minimum inhibitory concentrations (MICs) of NG isolates were determined by the agar dilution method. ^[49] An agar dilution method was used to demonstrate the  antimicrobial activities of the pyrazolone derivatives and their minimum inhibitory concentration (MIC) values calculated. ^[50]

antimicrobial susceptibility testing

Antimicrobial susceptibility testing was performed using the reference agar dilution method according to Clinical and Laboratory Standards Institute (CLSI) guidelines with Brucella agar supplemented with 5 μg/mL hemin, 1 μg/mL vitamin K1 and 5% laked sheep blood. ^[1] Objective: In this study it was aimed to determine the antimicrobial susceptibility of Bacteroides fragilis group (BFG) bacteria using recently developed European Committee on Antimicrobial Susceptibility Testing (EUCAST) disc diffusion method and agar dilution method recommended by Clinical Laboratory Standart Institude (CLSI) for anaerobes and to investigate the agreement of the results of two tests. ^[2] Gonococcal isolates were sent to regional laboratories for antimicrobial susceptibility testing by agar dilution. ^[3] The aim of this study was to clarify the antimicrobial susceptibility profiles and genetic backgrounds of non-carbapenemase-producing reduced-carbapenem-susceptible (RCS) ECC blood isolates in Japan using agar dilution antimicrobial susceptibility testing, whole-genome sequencing, and quantitative polymerase chain reaction for assays of ampC, ompC and ompF transcripts. ^[4] Materials and Methods Colistin susceptibility of 200 nonduplicate clinical CRE isolates from December 2017 to June 2019 was determined by BMD, agar dilution (AD), E test, and rapid polymyxin NP test and interpreted as per the European Committee on Antimicrobial Susceptibility Testing. ^[5] β-Lactamase activity measurements and antimicrobial-susceptibility testing using agar dilution were also applied. ^[6] The spectrum of resistance encoded by the cfr(C) gene was further investigated by agar dilution antimicrobial susceptibility testing. ^[7] Methods: Antimicrobial susceptibility testing was performed with agar dilution method by using 360 ESBLs-producing E. ^[8] Antimicrobial susceptibility testing was performed by the agar dilution method except for colistin and tigecycline; for colistin and tigecycline, testing was conducted by the broth microdilution method. ^[9] BackgroundAgar dilution is the reference method for antimicrobial susceptibility testing (AST) of anaerobes, but currently the only verified and published method utilises supplemented brucella agar, which is not readily available in the UK. ^[10] The spectrum of resistance encoded by the cfr(C) gene was further investigated by agar dilution antimicrobial susceptibility testing. ^[11] And antimicrobial susceptibility testing was performed using the agar dilution method. ^[12] Antimicrobial susceptibility testing for 13 antimicrobial agents was performed by the agar dilution method, and the contribution of efflux pumps to carbapenem resistance was analysed. ^[13] We performed PCRs for the toxin gene, PCR ribotyping, multilocus sequence typing (MLST), antimicrobial susceptibility testing by agar dilution according to the recommendations of the CLSI and detection of antimicrobial resistance genes such as ermB, catD, tetM, vanZ and nimR by PCR. ^[14] The antimicrobial activities of crude extracts of petroleum ether, diethyl ether, chloroform, acetone, ethanol and methanol were investigated using agar well disc diffusion and agar dilution antimicrobial susceptibility testing methods. ^[15] Assess performance of broth microdilution (BMD) as well as agar dilution methods for antimicrobial susceptibility testing of Staphylococci using tetrazolium salt. ^[16]

minimal inhibitory concentration

agalactiae clinical isolates (n = 136) were collected and the minimal inhibitory concentrations of antimicrobials were determined by agar dilution methodology. ^[1] Selected plant extracts and antimicrobial drugs were tested in vitro with agar dilution method on 35 bacteria strains and 1 yeast to evaluate their Minimal Inhibitory Concentration (MIC). ^[2] LVX minimal inhibitory concentration (MIC) was determined by the agar dilution method. ^[3] The minimal inhibitory concentrations (MICs) were tested in a central laboratory using the agar dilution method recommended by the Clinical and Laboratory Standards Institute. ^[4] This study tested the equivalency of minimal inhibitory concentrations (MICs) obtained by 2 reference susceptibility testing methods, agar dilution (AD) and broth microdilution (BMD), for gepotidacin against Gram-positive and Gram-negative organisms. ^[5] MethodsWe used the agar dilution method to determine the minimal inhibitory concentration of 5 alternative antibiotics against 42 strains from Nepal and 56 from Bangladesh and performed whole genome mutation analysis. ^[6] Minimal inhibitory concentration of Vancomycin among vancomycin resistant isolates was assayed by agar dilution method according to CLSI (2013) guidelines. ^[7] Antimicrobial activity was analyzed by the minimal inhibitory concentration (MIC) determination by means the agar dilution method. ^[8] Methods Minimal Inhibitory Concentration (MIC) was determined by agar dilution and the molecular epidemiology was evaluated by NG-MAST. ^[9] The study measured creatinine at enrollment and TOC, serum gentamicin concentration post-dose to establish peak concentration (Cmax), and standard antimicrobial minimal inhibitory concentrations (MIC) by agar dilution. ^[10] Methods: The anti-microbial activity and minimal inhibitory concentration were measured the agar dilution method. ^[11] Minimal inhibitory concentration (MIC), determined by agar dilution, revealed 42% to 79% of isolates resistant to ciprofloxacin, and 4% to 9. ^[12] ESBL detection was confirmed by minimal inhibitory concentration method using agar dilution technique for those who screened positive by ceftazidime (30 μg) disc. ^[13]

disk diffusion method

Antibiotic susceptibility testing was conducted by using disk-diffusion method, agar dilution method, and Epsilometer test (E-test). ^[1] Here we describe three commonly used methods for testing susceptibility to antimicrobial agents in Salmonella, including the disk diffusion method, the broth microdilution method, and the agar dilution method. ^[2] Susceptibility test to Mupirocin was performed by the disk diffusion method and to CHX by the agar dilution technique. ^[3] psychrophilum were tested on Diluted (1:5) Cation-Adjusted Mueller-Hinton (DCAMH) agar using a disk diffusion method and an agar dilution method, respectively. ^[4] Antimicrobial susceptibility of these isolates was determined by agar dilution and disk diffusion methods. ^[5] Methods Seven commonly used drug susceptibility testing methods were compared and evaluated for the ability to determine CRE tigecycline susceptibility: broth microdilution (BMD), agar dilution method (ADM), disk diffusion method, Etest, MicroScan, Vitek2 COMPACT, and BD Phoenix 100. ^[6] Disk diffusion method was performed to differentiate between MDR and non-MDR isolates and tolerance to CHX was determined by a modified agar dilution method. ^[7] In vitro antimicrobial susceptibility was determined by the disk diffusion method; the minimum inhibitory concentration and minimum bactericidal concentration were determined by agar dilution technique. ^[8] aureus strains was determined using the agar disk diffusion method with a cefoxitin disk and the agar dilution method with oxacillin. ^[9] Antimicrobial susceptibility was preliminarily tested by the disk diffusion method, and further was verified with the E-test and agar dilution methods. ^[10] aureus isolates were examined by agar dilution and Kirby-Bauer disk diffusion methods, respectively. ^[11] To evaluate the reliability of routine detection of antimicrobial susceptibility of Neisseria gonorrhoeae by disk diffusion method, six kinds of discs targeted at the specific antibiotics (penicillin, ceftriaxone, cefixime, tetracycline, ciprofloxacin and spectinomycin) from two domestic and one foreign manufacturer were included into the evaluation using agar dilution method as a reference method to validate the concordance between the two methods. ^[12] The antibacterial activities of valonia and shell extracts were evaluated by the agar disk diffusion method and agar dilution method. ^[13]

broth dilution method

The anti-anaerobic bacterial potential was detected utilizing a Brucella-agar dilution assay against four standard bacterial strains, whereas the antifungal potential was estimated via a Sabouraud-dextrose broth dilution method against two standard fungal strains. ^[1] Methods Micro-broth dilution method, agar dilution method, checkerboard assay, scanning electron microscopy (SEM), and transmission electron microscopy (TEM) were used for the assessment of anti-bacterial activity. ^[2] The susceptibility of all the recovered bacterial isolates (n=600) to different antibiotics and different biocides (BAC, TCS, phenol, hydrogen peroxide, chlorohexidine and citric acid) was performed using agar dilution and broth dilution methods, respectively. ^[3] By using the agar dilution method and broth dilution method, we determined the minimum inhibitory concentration (MIC) of candidate antibiotics. ^[4] Antibiotic susceptibility tests were conducted with agar dilution or broth dilution methods as recommended by US Clinical and Laboratory Standards Institute(CLSI)2018. ^[5] Both broth dilution method and agar dilution method were used to obtain the MIC and MBC values of the targeted QACs. ^[6] Antibacterial and anti-yeast activities were determined by Micro broth dilution method, while anti-mold activities were determined by agar dilution method. ^[7]

broth microdilution method

MICs were determined using the broth microdilution method, the agar dilution method (fosfomycin) or MIC gradient strips (plazomicin). ^[1] Antibiotic susceptibilities were determined by an agar dilution and a broth microdilution method. ^[2] The reliability of broth microdilution methods has not been assessed yet using the agar dilution reference method. ^[3] Antibiotic susceptibilities for isolates co-harboring carbapenemase genes and mcr were determined by an agar dilution or a broth microdilution method. ^[4] The susceptibility of the isolates to antifungal agents was then evaluated using the agar dilution and broth microdilution methods. ^[5] In this study, the broth microdilution method for colistin and the agar dilution method for fosfomycin were used for a total of 147 multidrug-resistant (MDR) or extensively drug-resistant (XDR) strains of CRE. ^[6] The MICs of levonadifloxacin and comparator antibiotics were determined using the reference agar dilution method and broth microdilution method. ^[7]

polymerase chain reaction

coli isolates causing BSI collected from this study, polymerase chain reaction (PCR) was used to detect ESBLs and carbapenemase genes, and minimum inhibitory concentrations (MICs) were determined with agar dilution method. ^[1] aureus SCVs and corresponding parental strains were investigated by classic microbiological methods, agar dilution method, polymerase chain reaction (PCR). ^[2] METHODS A collection of 761 enterococcal clinical isolates were studied, investigated the susceptibility of fosfomycin using the agar dilution method and analyzed for mechanisms of resistance by polymerase chain reaction and quantitative real-time PCR. ^[3] The presence of class D and B β-lactamases was investigated using polymerase chain reaction, and antimicrobial susceptibility testing was performed by agar dilution and broth microdilution. ^[4] actinomycetemcomitans strains were serotyped using a multiplex polymerase chain reaction, and minimal inhibitory concentration (MIC) values of amoxicillin were determined using the agar dilution method (range 0. ^[5]

agar well diffusion

Methods: The composition of essential oil of Varlangy, Seremna, and Sawney was analyzed using gas chromatography–mass spectrometry and comparative antimicrobial activity of oils was explored using agar well diffusion and agar dilution method. ^[1] 49530 2 bacteriaPseudomonas aeruginosa, Staphylococus aureus and Escherichia coli isolated from cooking wares in homes were investigated using agar well diffusion and agar dilution method to test for the antibacterial activity of fresh Allium cepa (onion) extract. ^[2] officinalis EO were assessed using disk diffusion agar, well diffusion agar, micro-well dilution, agar dilution and minimum bactericidal concentration (MBC) methods. ^[3]

minimum bactericidal concentration

52451 2 Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the extracts were carried out by agar dilution and agar diffusion techniques, respectively. ^[1]

Reference Agar Dilution

Antimicrobial susceptibility testing was performed using the reference agar dilution method according to Clinical and Laboratory Standards Institute (CLSI) guidelines with Brucella agar supplemented with 5 μg/mL hemin, 1 μg/mL vitamin K1 and 5% laked sheep blood. ^[1] Clinical isolates of Enterobacterales other than Escherichia coli (EOTEC), non-fermenting Gram-negative bacilli, and Gram-positive cocci were tested for susceptibility to fosfomycin using Etest® and reference agar dilution. ^[2] Minimum inhibitory concentrations (MICs) of amoxicillin, piperacillin, cefotaxime, imipenem, clindamycin, vancomycin, linezolid, moxifloxacin, rifampicin, and metronidazole were determined by the reference agar dilution method. ^[3] The MICs obtained by Raman-2nd derivative transformation agreed with the reference agar dilution method for all isolates. ^[4] Objective: To compare the in-vitro efficacy by determining Minimum Inhibitory Concentration of Vancomycinusing the reference Agar Dilution to the E-Strip in Methicillin Resistant Staphylococcus aureus isolates. ^[5] Results from the disk diffusion and rapid fosfomycin NP methods were compared with the reference agar dilution method for Escherichia coli and Klebsiella spp. ^[6] The MICs of levonadifloxacin and comparator antibiotics were determined using the reference agar dilution method and broth microdilution method. ^[7] Fosfomycin susceptibility testing with Sensititre, Vitek2, Etest, Mic Strip Test and disk diffusion methodologies was compared versus reference agar dilution method (AD) with 78 clinical isolates of KPC-producing Klebsiella pneumoniae. ^[8]

Standard Agar Dilution

coli as Gram negative by using standard agar dilution (plate count) method to inhibit microbial growth in water treatment. ^[1] using the standard agar dilution susceptibility test. ^[2] Minimum inhibitory concentration for Zink O and Silver O nanoparticles was detected by using standard agar dilution method. ^[3] Antibiotic susceptibility was determined by the automated Vitex 2® system and using standard agar dilution test. ^[4] Standard agar dilution was used to screen chlorhexidine acetate-resistant isolates. ^[5] Results Our assay had 100% categorical agreement with the gold-standard agar dilution AST when N. ^[6]

Modified Agar Dilution

glabrata using a modified agar dilution method. ^[1] Disk diffusion method was performed to differentiate between MDR and non-MDR isolates and tolerance to CHX was determined by a modified agar dilution method. ^[2] parapsilosis) by modified agar dilution method. ^[3] Antimicrobial activity was determined with 9 different bacteria and fungi strain ( Staphylococcus aureus, Staphylococcus aureus MRSA, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii, Candida albicans, Candida krusei and Candida glabrata ) using modified agar dilution method. ^[4]

An Agar Dilution

An agar dilution method was performed to evaluate the in vitro activities of imipenem monotherapy and imipenem-relebactam combination. ^[1] An agar dilution test was used to determine the minimum inhibitory concentration and susceptibility profiles against doxycycline, ciprofloxacin, nalidixic acid, enrofloxacin, chloramphenicol and erythromycin. ^[2] An agar dilution method was used to demonstrate the  antimicrobial activities of the pyrazolone derivatives and their minimum inhibitory concentration (MIC) values calculated. ^[3]

Bsac Agar Dilution

These were categorised by carbapenemase-gene detection and interpretive reading of phenotypes, with MICs determined by BSAC agar dilution. ^[1] coli from bacteraemias, determined MICs by BSAC agar dilution, and undertook genomic sequencing with Illumina methodology. ^[2] Imipenem MICs were determined centrally by BSAC agar dilution, with relebactam at a fixed concentration (4 mg/L). ^[3]

Clsi Agar Dilution

MICs were determined by CLSI agar dilution. ^[1] METHODS Intestinal contents from cattle, pigs and chickens were randomly sampled (five or six countries/host; at least four abattoirs/country; one sample/animal/farm) for isolation of enterococci; antimicrobial susceptibilities were centrally assessed by CLSI agar dilution. ^[2] MICs were determined by CLSI agar dilution. ^[3]

Fold Agar Dilution

Antibiotic susceptibilities of 14 antibacterial agents to the bacteria were assessed by minimum inhibitory concentration (MIC) using two-fold agar dilution test which was recommended by the Clinical and Laboratory Standard Institute (CLSI). ^[1] We used two-fold agar dilution method to determine the minimum inhibitory concentration of five alternative antibiotics in 63 Dominican strains. ^[2] The MICs were determined by using a 2-fold agar dilution method. ^[3]

Employing Agar Dilution

Further the compounds were screened for in-vitro antimicrobial activity employing agar dilution method. ^[1] AgNPs were prepared and checked for their antimicrobial activity by the use of various concentrations employing agar dilution method. ^[2]

7h10 Agar Dilution

tuberculosis isolates from the Democratic Republic of the Congo, Ivory Coast, Peru, Thailand, and Switzerland by quantitative phenotypic DST for 11 antituberculous drugs using the BD Bactec MGIT 960 system and 7H10 agar dilution to generate a cross-validated phenotypic DST readout. ^[1] We measured rifampicin minimum inhibitory concentrations (MICs) using Middlebrook 7H10 agar dilution and BACTEC MGIT 960. ^[2]

agar dilution method

Aim: To determine the fosfomycin sensitivity pattern along with Minimum Inhibitory Concentration (MIC) against uropathogens by agar dilution method. ^[1] hydrophila SS332 were measured by the agar dilution method. ^[2] Antimicrobial susceptibility testing was performed using the reference agar dilution method according to Clinical and Laboratory Standards Institute (CLSI) guidelines with Brucella agar supplemented with 5 μg/mL hemin, 1 μg/mL vitamin K1 and 5% laked sheep blood. ^[3] coli isolates causing BSI collected from this study, polymerase chain reaction (PCR) was used to detect ESBLs and carbapenemase genes, and minimum inhibitory concentrations (MICs) were determined with agar dilution method. ^[4] Antibacterial test using broth and agar dilution method was performed in a total of 5 treatment groups with 6 repetitions. ^[5] Agar well diffusion and agar dilution methods were used to evaluate antibacterial activities and minimum inhibitory concentrations (MIC). ^[6] Antibiotic susceptibility testing was performed using an agar dilution method. ^[7] Antibiotic