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10.1016/j.yexcr.2019.111782

Here, we assessed the molecular and functional phenotype of vascular smooth muscle cells (VSMCs) cultured as 3D multilayered aggregates.

Assembly of vascular smooth muscle cells in 3D aggregates provokes cellular quiescence.

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10.1111/rda.13481

It is also known to support excellent differentiation of 3D multilayered skin models.

Air-liquid interface cell culture: From airway epithelium to the female reproductive tract.

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10.1186/s12885-019-6038-x

MethodsWe have recently established 3D Multilayered Cell Cultures (MCCs) of human NSCLC (A549) cells grown at the Air-Liquid Interface (ALI) as the first in vitro tool for screening the efficacy of inhaled anti-cancer drugs.

ALI multilayered co-cultures mimic biochemical mechanisms of the cancer cell-fibroblast cross-talk involved in NSCLC MultiDrug Resistance

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10.1021/ACSBIOMATERIALS.8B01647

In order to improve the biological responses of these fibrous structures, we designed and fabricated 3D multilayered composite scaffolds, where an electrospun nanofibrous substrate was coated with a thin layer of cell-laden hydrogel.

Mechanical and Biochemical Stimulation of 3D Multilayered Scaffolds for Tendon Tissue Engineering.

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3d Multilayered
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What is/are 3d Multilayered?

3d Multilayered - Here, we assessed the molecular and functional phenotype of vascular smooth muscle cells (VSMCs) cultured as 3D multilayered aggregates. [1] It is also known to support excellent differentiation of 3D multilayered skin models. [2] MethodsWe have recently established 3D Multilayered Cell Cultures (MCCs) of human NSCLC (A549) cells grown at the Air-Liquid Interface (ALI) as the first in vitro tool for screening the efficacy of inhaled anti-cancer drugs. [3] In order to improve the biological responses of these fibrous structures, we designed and fabricated 3D multilayered composite scaffolds, where an electrospun nanofibrous substrate was coated with a thin layer of cell-laden hydrogel. [4]