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Nature Protocols aims to publish the protocols being used to answer outstanding biological and biomedical science research questions, including methods grounded in physics and chemistry that have a practical application to the study of biological problems. As the main audience for Nature Protocols articles is research scientists, we only publish protocols that have research applications; thus, we do not publish protocols where the main application is to make decisions that influence patient management and treatment. Specific techniques of interest include, but are not limited to, protocols relating to: Biochemistry; Cell biology; Cell culture; Chemical modification; Computational biology; Developmental biology; Epigenomics; Genetic analysis; Genetic modification; Genomics; Imaging; Immunology; Isolation, purification and separation; Lipidomics; Metabolomics; Microbiology; Model organisms; Nanotechnology; Neuroscience; Nucleic-acid based molecular biology; Pharmacology; Plant biology; Protein analysis; Proteomics; Spectroscopy; Structural biology; Synthetic chemistry; Tissue culture; Toxicology; Virology.